SRR3553949_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613369
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	12554	2.0467288043575724	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	9666	1.5758866196367929	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	9588	1.5631699678333923	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3936	0.64170181407929	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT	2951	0.48111332656198796	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2748	0.4480174250736506	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	2613	0.4260078354139189	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	1969	0.32101394103712444	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1810	0.29509153543788486	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA	1802	0.2937872634580489	No Hit
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	1790	0.29183085548829496	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1167	0.19026067505856994	No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	926	0.15096948166601182	TruSeq Adapter, Index 20 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	832	0.13564428590293934	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	709	0.11559110421296152	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	697	0.1136346962432076	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAAGGTCC	640	0.10434175838687641	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	639	0.10417872438939692	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	638	0.10401569039191744	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	631	0.10287445240956096	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAGT	35	1.210683E-7	45.000004	32
CGTTCTA	35	1.210683E-7	45.000004	27
ATCGTGA	35	1.210683E-7	45.000004	30
CGCATCG	20	7.0307107E-4	45.0	21
GATCACG	25	3.8886712E-5	45.0	20
GTCGTTT	25	3.8886712E-5	45.0	9
ACGTCGC	20	7.0307107E-4	45.0	20
GTATACG	20	7.0307107E-4	45.0	1
TATAGCG	20	7.0307107E-4	45.0	1
CGATGTT	20	7.0307107E-4	45.0	10
ATAACGC	25	3.8886712E-5	45.0	11
TAGGCCG	20	7.0307107E-4	45.0	4
CACGTTG	25	3.8886712E-5	45.0	1
CACGTAG	25	3.8886712E-5	45.0	1
ATCGGCA	25	3.8886712E-5	45.0	27
ATCGCAA	20	7.0307107E-4	45.0	43
CCGCACG	25	3.8886712E-5	45.0	1
GCATCGT	20	7.0307107E-4	45.0	35
ACGACAG	20	7.0307107E-4	45.0	1
TGCGTAG	20	7.0307107E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content