##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613369
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.857403944444535	31.0	31.0	34.0	30.0	34.0
2	31.948419303877436	33.0	31.0	34.0	30.0	34.0
3	31.993486791800695	33.0	31.0	34.0	30.0	34.0
4	35.625025066477114	37.0	35.0	37.0	33.0	37.0
5	35.46259429478829	37.0	35.0	37.0	33.0	37.0
6	35.43697839310431	37.0	35.0	37.0	33.0	37.0
7	35.64860141285262	37.0	35.0	37.0	35.0	37.0
8	35.19080520861015	37.0	35.0	37.0	32.0	37.0
9	37.04340128046901	39.0	37.0	39.0	33.0	39.0
10	36.75790103510285	39.0	37.0	39.0	32.0	39.0
11	36.73735386040051	39.0	35.0	39.0	32.0	39.0
12	36.62917591205294	39.0	35.0	39.0	32.0	39.0
13	36.46040148752219	39.0	35.0	39.0	32.0	39.0
14	37.53947460663972	39.0	36.0	41.0	32.0	41.0
15	37.586441440633614	39.0	36.0	41.0	32.0	41.0
16	37.797117232856564	39.0	36.0	41.0	33.0	41.0
17	37.60308232075635	39.0	36.0	41.0	32.0	41.0
18	37.6535185182166	39.0	36.0	41.0	33.0	41.0
19	37.60452843231399	39.0	36.0	41.0	33.0	41.0
20	37.37457876090901	39.0	35.0	41.0	32.0	41.0
21	37.432225952077786	39.0	35.0	41.0	32.0	41.0
22	37.42209501947441	39.0	35.0	41.0	32.0	41.0
23	37.23504122314626	39.0	35.0	41.0	32.0	41.0
24	37.269182172558445	39.0	35.0	41.0	32.0	41.0
25	37.24026972344543	39.0	35.0	41.0	32.0	41.0
26	37.1313972502686	39.0	35.0	41.0	32.0	41.0
27	37.022167732637286	39.0	35.0	41.0	31.0	41.0
28	36.91595923497927	39.0	35.0	41.0	31.0	41.0
29	36.89433277521361	39.0	35.0	41.0	31.0	41.0
30	36.810388200251396	39.0	35.0	40.0	31.0	41.0
31	36.64235068938926	39.0	35.0	40.0	30.0	41.0
32	36.46565607326096	39.0	35.0	40.0	30.0	41.0
33	36.33669944193463	38.0	35.0	40.0	30.0	41.0
34	36.1403364043504	38.0	35.0	40.0	30.0	41.0
35	36.09270765232674	38.0	35.0	40.0	30.0	41.0
36	36.056219013350855	38.0	35.0	40.0	30.0	41.0
37	35.980675580278756	38.0	35.0	40.0	30.0	41.0
38	35.9357629746531	38.0	35.0	40.0	29.0	41.0
39	35.80594226314013	38.0	35.0	40.0	29.0	41.0
40	35.72820276212199	38.0	35.0	40.0	29.0	41.0
41	35.54632529521381	38.0	34.0	40.0	27.0	41.0
42	35.621602982870016	38.0	35.0	40.0	28.0	41.0
43	35.58511434389413	38.0	35.0	40.0	28.0	41.0
44	35.54364338595527	38.0	34.0	40.0	28.0	41.0
45	35.49783083266354	38.0	34.0	40.0	28.0	41.0
46	35.34300233627718	38.0	34.0	40.0	27.0	41.0
47	35.13569808712211	37.0	34.0	40.0	27.0	41.0
48	35.169659699137064	37.0	34.0	40.0	27.0	41.0
49	35.15963962965197	37.0	34.0	40.0	27.0	41.0
50	35.0484732681306	37.0	34.0	40.0	27.0	41.0
51	33.57971302755764	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	3.0
13	9.0
14	12.0
15	26.0
16	49.0
17	111.0
18	209.0
19	467.0
20	908.0
21	1722.0
22	2779.0
23	3621.0
24	4255.0
25	4867.0
26	5699.0
27	6438.0
28	7644.0
29	9514.0
30	12454.0
31	16396.0
32	21287.0
33	29082.0
34	46357.0
35	57968.0
36	54711.0
37	75746.0
38	116266.0
39	134720.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.8777554783499	16.911516558547955	20.954107560049497	13.256620403052649
2	26.856427370799636	32.88819617554849	23.508198164563257	16.747178289088623
3	28.583283472102437	31.344101185420197	24.65579447282142	15.41682086965595
4	25.426619212904466	23.901925268476234	32.567182234511364	18.104273284107936
5	29.78924595145826	27.62904548485496	22.447335942964187	20.134372620722598
6	22.191861668913816	39.75404691140243	23.50102466867416	14.553066751009588
7	73.39497105331375	5.6419545167753835	15.626645624412058	5.3364288054988105
8	72.24183158914128	11.846539358852501	9.15794570641816	6.753683345588056
9	67.52069309013008	7.395874261659784	10.673020644995102	14.410412003215031
10	40.60752988820759	22.81465153928549	18.174703971019078	18.40311460148785
11	32.60712556389384	24.23924260926131	23.30342746372901	19.85020436311584
12	26.629810114303137	20.109428419108237	29.805386317208725	23.4553751493799
13	24.622372503338124	24.118271383131525	32.7163583422051	18.542997771325254
14	19.47995415483991	31.950098554051475	26.1451100397966	22.424837251312017
15	16.84907453751331	24.934745642508833	36.980675580278756	21.235504239699104
16	18.604298554377543	24.160497188478715	28.88245085747731	28.35275339966643
17	18.51022793783188	26.062288769077014	29.46285188850431	25.964631404586797
18	20.25387654087507	24.496836325278913	29.177705426912677	26.071581706933344
19	21.457067442273736	27.430470075924934	25.53471075323337	25.57775172856796
20	23.512926150490163	25.698396886702785	28.9501099664313	21.838566996375754
21	22.222838128434923	31.24579168494006	25.84626872241669	20.68510146420833
22	19.267520856124126	23.918717770216624	27.968482267607264	28.845279106051986
23	20.881883499166083	29.61528867614764	26.3700969563183	23.132730868367982
24	22.161863413377592	25.68665843888426	26.362597392434246	25.788880755303907
25	19.367623730576536	31.986618169486885	24.101804949386096	24.543953150550486
26	18.397408411576066	28.439324452328048	28.19265401414157	24.970613121954322
27	22.662703853634596	28.52638460698209	24.222124039525962	24.588787499857347
28	17.734349143826964	29.574041074785324	29.56931308885842	23.122296692529293
29	23.144795384181464	27.662141386343293	25.055390800643657	24.137672428831582
30	22.08849811451182	32.39697474114277	24.665739546667666	20.84878759767774
31	24.033656738439667	29.395355813547795	21.44337258648546	25.127614861527075
32	27.789633972372258	30.055154401347313	20.551413586275146	21.60379804000528
33	25.101692455927832	26.251897308145665	20.825962838030616	27.82044739789588
34	21.849979376199318	28.271236400926686	25.997890340072615	23.88089388280138
35	22.630912224126096	26.586769138968545	22.928286235528695	27.85403240137666
36	22.260662015850166	31.5682729319545	22.341689912597474	23.82937513959786
37	23.23560532077754	27.986579041327488	26.619538972461925	22.158276665433043
38	23.7527491607825	28.751045455508837	21.583744858315303	25.91246052539336
39	26.27700454375751	23.740684644969015	26.36471683440148	23.617593976871998
40	23.860025531124005	23.34597933707116	26.898327108151864	25.895668023652973
41	18.98302653052241	25.62291214586978	25.339559058250416	30.054502265357396
42	20.473483335479948	24.55161574843202	27.306401203843038	27.668499712244994
43	23.296090933842432	23.826929629635668	24.68024957244334	28.196729864078556
44	23.38853121041331	24.298423950346365	27.081251253323856	25.231793585916474
45	21.86644580994475	23.50004646468928	26.712957453017676	27.920550272348294
46	26.929303567672967	26.09408039858552	24.373419589186934	22.603196444554584
47	20.30229763812648	23.904207744440946	32.211115984016146	23.582378633416425
48	22.050674227096575	24.118760485123964	26.554162339472647	27.27640294830681
49	21.538584441013484	21.263546087265578	31.434911122016274	25.76295834970466
50	22.602707342562145	21.43407964862913	28.397424714975816	27.565788293832917
51	21.934267952896217	22.10757309221692	25.958273078685096	29.999885876201766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1096.0
1	1214.0
2	1332.0
3	1411.5
4	1491.0
5	1136.5
6	782.0
7	824.5
8	867.0
9	959.0
10	1051.0
11	1078.5
12	1106.0
13	1128.5
14	1151.0
15	1151.5
16	1152.0
17	1120.0
18	1088.0
19	1293.0
20	1498.0
21	1675.0
22	1852.0
23	1802.0
24	1752.0
25	2681.5
26	3840.5
27	4070.0
28	4587.0
29	5104.0
30	5448.5
31	5793.0
32	7056.0
33	8319.0
34	9630.5
35	10942.0
36	12336.0
37	13730.0
38	14782.0
39	15834.0
40	18789.5
41	21745.0
42	25935.5
43	30126.0
44	34869.5
45	39613.0
46	65794.0
47	91975.0
48	80899.0
49	69823.0
50	66514.0
51	63205.0
52	53633.5
53	44062.0
54	38561.5
55	33061.0
56	29859.5
57	26658.0
58	25058.5
59	23459.0
60	21836.5
61	20214.0
62	18894.0
63	17574.0
64	15585.0
65	13596.0
66	11543.0
67	9490.0
68	8420.0
69	7350.0
70	6722.0
71	6094.0
72	5143.0
73	4192.0
74	3571.5
75	2607.5
76	2264.0
77	1736.5
78	1209.0
79	877.0
80	545.0
81	411.5
82	278.0
83	187.5
84	97.0
85	90.5
86	84.0
87	53.0
88	22.0
89	17.0
90	12.0
91	23.5
92	35.0
93	23.0
94	11.0
95	6.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	613369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.713873227816627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3688003765047	20.610475057392524
2	10.410440198288121	5.770272398022463
3	3.8882099277195215	3.232720710598706
4	1.9047483701010282	2.1115181943948094
5	1.1823377733907303	1.638357958210484
6	0.8175063182793527	1.3593759880639855
7	0.5850878991263877	1.135053630446279
8	0.4889766647297145	1.084114983814392
9	0.37497189257735236	0.9352731145394896
>10	5.038217201392293	33.76794275943516
>50	0.8586721548501844	15.050581521062432
>100	0.06727740594702476	3.3252197616300485
>500	0.007671984888695806	1.3771260829730465
>1k	0.005311374153712481	3.3996369074439126
>5k	0.0011803053674916625	3.1490719241761327
>10k+	5.901526837458313E-4	2.053259007796155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	12554	2.0467288043575724	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	9666	1.5758866196367929	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	9588	1.5631699678333923	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3936	0.64170181407929	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT	2951	0.48111332656198796	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2748	0.4480174250736506	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	2613	0.4260078354139189	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	1969	0.32101394103712444	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1810	0.29509153543788486	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA	1802	0.2937872634580489	No Hit
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	1790	0.29183085548829496	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1167	0.19026067505856994	No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	926	0.15096948166601182	TruSeq Adapter, Index 20 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	832	0.13564428590293934	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	709	0.11559110421296152	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	697	0.1136346962432076	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAAGGTCC	640	0.10434175838687641	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	639	0.10417872438939692	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	638	0.10401569039191744	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	631	0.10287445240956096	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30715605125136747	0.0
2	0.0	0.0	0.0	1.704194375653155	0.0
3	0.0	0.0	0.0	2.139495148923405	0.0
4	0.0	0.0	0.0	2.965262346157044	0.0
5	0.0	0.0	0.0	5.979923993550375	0.0
6	0.0	0.0	0.0	7.18767984687847	0.0
7	0.0	0.0	0.0	8.614227324824046	0.0
8	0.0	0.0	0.0	10.464500162218828	0.0
9	0.0	0.0	0.0	11.115820982149408	0.0
10	0.0	0.0	0.0	14.590727604427352	0.0
11	0.0	0.0	0.0	16.176559297910394	0.0
12	0.0	0.0	0.0	19.266216584144292	0.0
13	0.0	0.0	0.0	19.911016044175692	0.0
14	0.0	0.0	0.0	20.219313333409417	0.0
15	0.0	0.0	0.0	20.890198233037534	0.0
16	1.630339974794944E-4	0.0	0.0	21.737485917938468	0.0
17	1.630339974794944E-4	0.0	0.0	22.718461480772586	0.0
18	1.630339974794944E-4	0.0	0.0	23.914641920279635	0.0
19	1.630339974794944E-4	0.0	0.0	25.010556451336797	0.0
20	1.630339974794944E-4	0.0	0.0	25.659757829300144	0.0
21	1.630339974794944E-4	0.0	0.0	26.227931310516183	0.0
22	1.630339974794944E-4	0.0	0.0	26.88642562633586	0.0
23	1.630339974794944E-4	0.0	0.0	27.477260833201548	0.0
24	1.630339974794944E-4	0.0	0.0	27.929517142209665	0.0
25	1.630339974794944E-4	0.0	0.0	28.312647036286478	0.0
26	3.260679949589888E-4	0.0	0.0	28.687299162494355	0.0
27	3.260679949589888E-4	0.0	0.0	29.1377620975302	0.0
28	3.260679949589888E-4	0.0	0.0	29.516327039677584	0.0
29	3.260679949589888E-4	0.0	0.0	29.905652225658617	0.0
30	3.260679949589888E-4	0.0	0.0	30.360354044628927	0.0
31	3.260679949589888E-4	0.0	0.0	30.749842264607437	0.0
32	3.260679949589888E-4	0.0	0.0	31.12058157487581	0.0
33	3.260679949589888E-4	0.0	0.0	31.496864041058483	0.0
34	3.260679949589888E-4	0.0	0.0	31.854234563533534	0.0
35	3.260679949589888E-4	0.0	0.0	32.2520375173835	0.0
36	3.260679949589888E-4	0.0	0.0	32.62668964359138	0.0
37	3.260679949589888E-4	0.0	0.0	32.9747672282101	0.0
38	3.260679949589888E-4	0.0	0.0	33.32577942478345	0.0
39	8.15169987397472E-4	0.0	0.0	33.67499824738453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAGT	35	1.210683E-7	45.000004	32
CGTTCTA	35	1.210683E-7	45.000004	27
ATCGTGA	35	1.210683E-7	45.000004	30
CGCATCG	20	7.0307107E-4	45.0	21
GATCACG	25	3.8886712E-5	45.0	20
GTCGTTT	25	3.8886712E-5	45.0	9
ACGTCGC	20	7.0307107E-4	45.0	20
GTATACG	20	7.0307107E-4	45.0	1
TATAGCG	20	7.0307107E-4	45.0	1
CGATGTT	20	7.0307107E-4	45.0	10
ATAACGC	25	3.8886712E-5	45.0	11
TAGGCCG	20	7.0307107E-4	45.0	4
CACGTTG	25	3.8886712E-5	45.0	1
CACGTAG	25	3.8886712E-5	45.0	1
ATCGGCA	25	3.8886712E-5	45.0	27
ATCGCAA	20	7.0307107E-4	45.0	43
CCGCACG	25	3.8886712E-5	45.0	1
GCATCGT	20	7.0307107E-4	45.0	35
ACGACAG	20	7.0307107E-4	45.0	1
TGCGTAG	20	7.0307107E-4	45.0	1
>>END_MODULE