FastQCFastQC Report
Sat 18 Jun 2016
SRR3553947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587759
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC29020.49373978110075734TruSeq Adapter, Index 13 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC20520.3491226846377512No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC18510.31492499476826386TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT16280.2769842741667929TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC11580.1970195267107777TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.1759224444032333No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC7560.12862414697180308No Hit
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC7280.12386028967655112TruSeq Adapter, Index 16 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.11212078419896591No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTT207.0304703E-445.017
AACGTAT253.8884726E-545.010
TCGTTTG253.8884726E-545.01
CGTATTA253.8884726E-545.017
CACGGAT207.0304703E-445.040
TCCGATC207.0304703E-445.015
TAGACCG406.8048394E-945.01
TCCCTAT253.8884726E-545.026
TAGCCGT253.8884726E-545.044
TAGCCCG207.0304703E-445.016
ATCGTTC253.8884726E-545.014
ATCGTAA207.0304703E-445.016
TATTGCC207.0304703E-445.013
CCGTATT253.8884726E-545.016
CTCGACG207.0304703E-445.022
TATACGT207.0304703E-445.01
CGTCCAA207.0304703E-445.023
CCACGCG207.0304703E-445.01
ATACACT207.0304703E-445.020
ATACACG253.8884726E-545.01
Produced by FastQC (version 0.11.5)