SRR3553946_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286524
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1687	0.5887813935307339	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1380	0.48163504627884574	TruSeq Adapter, Index 21 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1248	0.4355656070695648	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1248	0.4355656070695648	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1170	0.4083427566277171	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	938	0.3273722271083748	TruSeq Adapter, Index 15 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	602	0.21010456366656896	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	568	0.1982381929611481	No Hit
AAAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	541	0.18881489857743156	No Hit
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	522	0.18218369141851992	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	398	0.13890633943404393	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	382	0.1333221649844341	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	380	0.13262414317823287	No Hit
GGACAGGAGGGCAGAGGGAGGGCAGGAGAGGGCAAGGGGATGAACATTGTT	294	0.10260920551158018	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	288	0.1005151400929765	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTC	30	2.159777E-6	45.000004	20
CGGGTCC	35	1.2076634E-7	45.000004	6
CGGGTAT	35	1.2076634E-7	45.000004	6
CGTGGGA	20	7.0244115E-4	45.000004	4
TTAGGAG	20	7.0244115E-4	45.000004	12
ACACGCC	20	7.0244115E-4	45.000004	28
ACGCCTC	25	3.8834507E-5	45.000004	45
CATATTA	25	3.8834507E-5	45.000004	28
CGTATGG	20	7.0244115E-4	45.000004	2
GTCGAGG	20	7.0244115E-4	45.000004	1
CACGGGT	25	3.8834507E-5	45.000004	4
TCTCTCG	20	7.0244115E-4	45.000004	12
ACTTGCG	35	1.2076634E-7	45.000004	1
TTTCGAG	20	7.0244115E-4	45.000004	1
TATGGAC	20	7.0244115E-4	45.000004	39
GGACTAC	30	2.159777E-6	45.000004	9
ATAGCAG	20	7.0244115E-4	45.000004	1
CACGCCC	20	7.0244115E-4	45.000004	12
GTGATTG	20	7.0244115E-4	45.000004	1
TTCGTCT	20	7.0244115E-4	45.000004	44