##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286524
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90006421800617	33.0	31.0	34.0	30.0	34.0
2	32.00247797741201	33.0	31.0	34.0	30.0	34.0
3	32.02969733774483	33.0	31.0	34.0	30.0	34.0
4	35.62203166226913	37.0	35.0	37.0	33.0	37.0
5	35.522081919839174	37.0	35.0	37.0	33.0	37.0
6	35.514581675531545	37.0	35.0	37.0	33.0	37.0
7	35.723970766846755	37.0	35.0	37.0	35.0	37.0
8	35.56145034970893	37.0	35.0	37.0	35.0	37.0
9	37.28830045650626	39.0	37.0	39.0	34.0	39.0
10	36.892857142857146	39.0	37.0	39.0	33.0	39.0
11	36.86325403805615	39.0	37.0	39.0	33.0	39.0
12	36.620461113205174	39.0	35.0	39.0	33.0	39.0
13	36.39983387081013	39.0	35.0	39.0	32.0	39.0
14	37.37753905432006	39.0	35.0	41.0	32.0	41.0
15	37.59910164593542	39.0	36.0	41.0	33.0	41.0
16	37.73237843950245	39.0	36.0	41.0	33.0	41.0
17	37.68494785777108	39.0	36.0	41.0	33.0	41.0
18	37.62345911686281	39.0	36.0	41.0	33.0	41.0
19	37.55959710181346	39.0	36.0	41.0	33.0	41.0
20	37.450286886962346	39.0	35.0	41.0	33.0	41.0
21	37.33717594337647	39.0	35.0	41.0	33.0	41.0
22	37.32736524689031	39.0	35.0	41.0	33.0	41.0
23	37.272811352626654	39.0	35.0	41.0	33.0	41.0
24	37.282381231589675	39.0	35.0	41.0	33.0	41.0
25	37.204701874886574	39.0	35.0	41.0	32.0	41.0
26	37.18019083916182	39.0	35.0	41.0	33.0	41.0
27	37.119236084935295	39.0	35.0	41.0	32.0	41.0
28	37.01039354469434	39.0	35.0	41.0	32.0	41.0
29	37.03485222878363	39.0	35.0	41.0	32.0	41.0
30	36.87544498890146	39.0	35.0	41.0	32.0	41.0
31	36.765014449051385	39.0	35.0	40.0	31.0	41.0
32	36.64728609121749	38.0	35.0	40.0	31.0	41.0
33	36.55588362580447	38.0	35.0	40.0	31.0	41.0
34	36.49420990911756	38.0	35.0	40.0	31.0	41.0
35	36.398877580935626	38.0	35.0	40.0	31.0	41.0
36	36.252830478424144	38.0	35.0	40.0	30.0	41.0
37	36.201459563596764	38.0	35.0	40.0	30.0	41.0
38	36.09437254819841	38.0	35.0	40.0	30.0	41.0
39	36.073725063170976	38.0	35.0	40.0	30.0	41.0
40	35.90304826122768	38.0	35.0	40.0	30.0	41.0
41	35.77769401516103	38.0	35.0	40.0	30.0	41.0
42	35.69448981586185	38.0	35.0	40.0	29.0	41.0
43	35.61914534210049	38.0	35.0	40.0	29.0	41.0
44	35.533093213832	38.0	35.0	40.0	29.0	41.0
45	35.5172446287222	38.0	34.0	40.0	29.0	41.0
46	35.37244000502576	37.0	34.0	40.0	28.0	41.0
47	35.25406597702112	37.0	34.0	40.0	28.0	41.0
48	35.1741110692298	37.0	34.0	40.0	28.0	41.0
49	35.15405690273764	37.0	34.0	40.0	28.0	41.0
50	35.01440717007999	37.0	34.0	40.0	27.0	41.0
51	33.486496768159036	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	1.0
13	4.0
14	9.0
15	21.0
16	35.0
17	74.0
18	149.0
19	247.0
20	401.0
21	617.0
22	926.0
23	1256.0
24	1667.0
25	2046.0
26	2392.0
27	2906.0
28	3568.0
29	4308.0
30	5628.0
31	6992.0
32	9426.0
33	13331.0
34	23467.0
35	32170.0
36	23312.0
37	33033.0
38	54184.0
39	64322.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.686560288143404	20.337563345478912	23.807080733202106	14.168795633175579
2	27.825243260599464	27.579539584816633	26.056456003685557	18.538761150898353
3	30.277044854881268	25.178344571484413	28.48382683475032	16.060783738884002
4	28.256620736831817	28.017897279110997	24.217517555248428	19.507964428808755
5	22.67000321090031	31.41342435537686	25.02757186134495	20.88900057237788
6	22.235484636540047	35.284304281665754	26.11613686811576	16.364074213678435
7	77.13036255252615	7.959542656112577	8.960505926205133	5.949588865156148
8	77.90551576831261	6.279753179489327	8.423029135430191	7.391701916767881
9	73.17432396588069	7.883458279236644	11.392413899010204	7.549803855872457
10	40.78960226717483	23.58196870070221	18.942566765785763	16.6858622663372
11	32.45277882481049	27.121288269045525	22.273177814074913	18.152755092069075
12	28.75221621923469	21.608312043668242	29.541678882048277	20.09779285504879
13	23.315324370733343	28.982912426184193	29.422317153187866	18.2794460498946
14	17.84178637740643	33.41186078653097	28.534433415699905	20.211919420362694
15	16.846058270860382	26.780653627619326	37.159190853122254	19.21409724839804
16	19.032611578785723	24.81921235219388	33.69072049810836	22.457455570912035
17	18.671734304979687	25.31864695453086	27.368387988440755	28.641230752048696
18	21.678812246094566	25.60832600410437	30.241096731861905	22.47176501793916
19	24.61853108291103	27.313942287557065	24.70543479778308	23.362091831748824
20	22.80751350672195	28.545601764599127	25.962572070751488	22.684312657927432
21	23.032974550124948	29.12705392916475	27.658765059820468	20.18120646088984
22	19.065418603677177	27.269268891960184	25.98490876854993	27.68040373581271
23	18.04142061397998	31.114322011419638	25.657536541441555	25.186720833158827
24	20.852354427552317	25.67952422833689	29.92105373371864	23.54706761039215
25	19.33974117351426	27.462969943181026	28.302341165138	24.894947718166716
26	17.878083511328892	30.005514372268987	28.16832097834736	23.948081138054754
27	20.389914980944006	29.80553112479234	26.35904845667379	23.44550543758987
28	17.94334855020871	27.56872024682051	30.087531934497637	24.400399268473148
29	20.12536471639374	26.211416844662228	28.837025868688137	24.826192570255895
30	21.49872262009465	29.227569069257726	26.701777163518585	22.57193114712904
31	23.46888916809761	29.153927768703497	22.513646326311235	24.86353673688766
32	22.507713140958526	29.159511943153106	24.513478801077746	23.819296114810626
33	21.581089193226397	30.05751699683098	22.527257751532158	25.83413605841047
34	19.06786167999888	27.974968938029626	26.840683502952633	26.116485879018857
35	18.604375200681268	26.474222054696988	28.244754366126397	26.676648378495344
36	23.138026831958232	29.022699669137666	25.451620108612193	22.387653390291913
37	20.788834443188005	28.225907777358966	27.921919280758328	23.063338498694698
38	19.65803911714202	30.56428082813307	25.553182281414472	24.22449777331044
39	23.693303178791307	28.295011936172887	24.21367843531432	23.79800644972149
40	24.3728274071282	25.01221538160852	28.613309879800646	22.001647331462635
41	19.393837863494856	26.91432480350686	26.797755161871255	26.894082171127025
42	21.70882718376122	26.351021205902477	27.397355893398114	24.542795716938198
43	21.2415015845095	25.10819337996119	28.755357317362595	24.894947718166716
44	20.653069201881866	25.55213524870517	27.462271921374825	26.33252362803814
45	21.29315519816839	25.19195599670534	26.175119710739764	27.339769094386508
46	23.386173584062767	26.90420348731694	26.455724476832653	23.253898451787634
47	20.840837067749995	25.36541441554634	29.313425751420475	24.480322765283187
48	19.68177185855286	24.622719213748237	29.126006896455447	26.56950203124346
49	22.145788834443188	22.18767014281526	30.603370049280336	25.06317097346121
50	20.89353771411819	22.695830017729755	30.413159107090504	25.997473161061553
51	19.81125490360319	24.184710530356966	26.067973363487873	29.93606120255197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1373.0
1	1363.5
2	1354.0
3	1238.0
4	1122.0
5	844.5
6	567.0
7	624.0
8	681.0
9	759.0
10	837.0
11	859.5
12	882.0
13	880.0
14	878.0
15	905.5
16	933.0
17	924.5
18	916.0
19	1045.0
20	1174.0
21	985.5
22	797.0
23	897.0
24	997.0
25	991.5
26	1145.5
27	1305.0
28	1501.5
29	1698.0
30	1995.0
31	2292.0
32	2699.5
33	3107.0
34	3601.5
35	4096.0
36	4775.0
37	5454.0
38	6685.5
39	7917.0
40	10131.5
41	12346.0
42	16112.0
43	19878.0
44	24189.0
45	28500.0
46	32118.0
47	35736.0
48	36087.5
49	36439.0
50	31595.0
51	26751.0
52	23033.0
53	19315.0
54	17314.5
55	15314.0
56	13728.5
57	12143.0
58	10939.0
59	9735.0
60	9274.5
61	8814.0
62	7927.0
63	7040.0
64	5729.5
65	4419.0
66	3801.0
67	3183.0
68	2782.5
69	2382.0
70	2035.5
71	1689.0
72	1454.0
73	1219.0
74	1035.5
75	705.0
76	558.0
77	411.5
78	265.0
79	231.5
80	198.0
81	183.0
82	168.0
83	150.0
84	132.0
85	75.0
86	18.0
87	16.0
88	14.0
89	13.5
90	13.0
91	12.0
92	11.0
93	14.0
94	17.0
95	11.5
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	286524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.674665996565736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28138797338474	25.350755957616116
2	10.025288642911926	6.751964931384456
3	3.8461538461538463	3.8855383842191227
4	1.9028667371432124	2.563136072370901
5	1.1058599175009847	1.8619731680417695
6	0.8343179321352321	1.68572266197596
7	0.5845407623903986	1.3778950454412195
8	0.49126298115788825	1.323449344557524
9	0.37000186555562464	1.121372031662269
>10	5.154115622991936	40.67826778908573
>50	0.3420185311858715	6.957532353310718
>100	0.0518209895736169	2.985788276025743
>500	0.0051820989573616895	1.1067135737320435
>1k	0.0051820989573616895	2.3498904105764264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1687	0.5887813935307339	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1380	0.48163504627884574	TruSeq Adapter, Index 21 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1248	0.4355656070695648	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1248	0.4355656070695648	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1170	0.4083427566277171	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	938	0.3273722271083748	TruSeq Adapter, Index 15 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	602	0.21010456366656896	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	568	0.1982381929611481	No Hit
AAAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	541	0.18881489857743156	No Hit
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	522	0.18218369141851992	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	398	0.13890633943404393	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	382	0.1333221649844341	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	380	0.13262414317823287	No Hit
GGACAGGAGGGCAGAGGGAGGGCAGGAGAGGGCAAGGGGATGAACATTGTT	294	0.10260920551158018	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	288	0.1005151400929765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3409836523292988	0.0
2	0.0	0.0	0.0	1.7925199983247477	0.0
3	0.0	0.0	0.0	2.3477963451578225	0.0
4	0.0	0.0	0.0	3.104800993983052	0.0
5	0.0	0.0	0.0	5.2965894654549	0.0
6	0.0	0.0	0.0	6.969049713113038	0.0
7	0.0	0.0	0.0	8.398598372213147	0.0
8	0.0	0.0	0.0	10.874481718808896	0.0
9	0.0	0.0	0.0	11.888009381413076	0.0
10	0.0	0.0	0.0	13.726249808044003	0.0
11	0.0	0.0	0.0	15.901634767070123	0.0
12	0.0	0.0	0.0	17.552805349639122	0.0
13	0.0	0.0	0.0	18.243497926875236	0.0
14	0.0	0.0	0.0	18.504558082394492	0.0
15	0.0	0.0	0.0	18.9059206209602	0.0
16	0.0	0.0	0.0	19.844410939397747	0.0
17	0.0	0.0	0.0	20.98462955982745	0.0
18	0.0	0.0	0.0	22.443495134788012	0.0
19	0.0	0.0	0.0	23.089514316427245	0.0
20	0.0	0.0	0.0	23.750889977802906	0.0
21	0.0	0.0	0.0	24.47648364534908	0.0
22	0.0	0.0	0.0	25.14937666652706	0.0
23	0.0	0.0	0.0	25.79155672823219	0.0
24	0.0	0.0	0.0	26.32065725733272	0.0
25	0.0	0.0	0.0	26.77157934413871	0.0
26	0.0	0.0	0.0	27.195627591405955	0.0
27	0.0	0.0	0.0	27.685987910262316	0.0
28	0.0	0.0	0.0	28.161340760285352	0.0
29	0.0	0.0	0.0	28.607027683544835	0.0
30	0.0	0.0	0.0	29.170331281149224	0.0
31	0.0	0.0	0.0	29.63032765143583	0.0
32	0.0	0.0	0.0	30.084041825466628	0.0
33	0.0	0.0	0.0	30.549622370202844	0.0
34	0.0	0.0	0.0	30.96320029037707	0.0
35	0.0	0.0	0.0	31.42808281330709	0.0
36	0.0	0.0	0.0	31.837123591741005	0.0
37	0.0	0.0	0.0	32.258379751783444	0.0
38	0.0	0.0	0.0	32.649969985062334	0.0
39	0.0	0.0	0.0	33.04365428375983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTC	30	2.159777E-6	45.000004	20
CGGGTCC	35	1.2076634E-7	45.000004	6
CGGGTAT	35	1.2076634E-7	45.000004	6
CGTGGGA	20	7.0244115E-4	45.000004	4
TTAGGAG	20	7.0244115E-4	45.000004	12
ACACGCC	20	7.0244115E-4	45.000004	28
ACGCCTC	25	3.8834507E-5	45.000004	45
CATATTA	25	3.8834507E-5	45.000004	28
CGTATGG	20	7.0244115E-4	45.000004	2
GTCGAGG	20	7.0244115E-4	45.000004	1
CACGGGT	25	3.8834507E-5	45.000004	4
TCTCTCG	20	7.0244115E-4	45.000004	12
ACTTGCG	35	1.2076634E-7	45.000004	1
TTTCGAG	20	7.0244115E-4	45.000004	1
TATGGAC	20	7.0244115E-4	45.000004	39
GGACTAC	30	2.159777E-6	45.000004	9
ATAGCAG	20	7.0244115E-4	45.000004	1
CACGCCC	20	7.0244115E-4	45.000004	12
GTGATTG	20	7.0244115E-4	45.000004	1
TTCGTCT	20	7.0244115E-4	45.000004	44
>>END_MODULE