SRR3553943_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639961
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2196	0.3431459104539183	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1322	0.20657508816943532	TruSeq Adapter, Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1320	0.20626256912530608	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1098	0.17157295522695915	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	952	0.14875906500552377	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	831	0.12985166283570404	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	776	0.12125738912214963	TruSeq Adapter, Index 13 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	754	0.11781967963672786	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	736	0.11500700823956461	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	25	3.8888596E-5	45.000004	12
ACGCATA	25	3.8888596E-5	45.000004	12
CGACAAT	35	1.2107921E-7	45.000004	20
CATAGAC	35	1.2107921E-7	45.000004	11
ACGTATA	25	3.8888596E-5	45.000004	11
CTCACGT	20	7.0309406E-4	45.0	45
GTACCGA	20	7.0309406E-4	45.0	9
ACGCATT	90	0.0	45.0	17
ACCGATC	20	7.0309406E-4	45.0	10
ATATGCG	45	3.8380676E-10	45.0	1
TCTAGCG	20	7.0309406E-4	45.0	1
ACTAGCA	20	7.0309406E-4	45.0	26
GAACCGA	20	7.0309406E-4	45.0	8
CCGCATT	20	7.0309406E-4	45.0	13
GCGATAT	30	2.163988E-6	44.999996	9
TCTACGC	105	0.0	42.857147	14
GTACGAG	60	3.6379788E-12	41.249996	1
TAAGGGT	160	0.0	40.78125	4
TACGCAT	105	0.0	40.714287	16
CTACGCA	105	0.0	40.714287	15