##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639961
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.941694884532026	33.0	31.0	34.0	30.0	34.0
2	32.02947523364705	33.0	31.0	34.0	30.0	34.0
3	32.07484206068807	33.0	31.0	34.0	30.0	34.0
4	35.666532804342765	37.0	35.0	37.0	33.0	37.0
5	35.54304402924553	37.0	35.0	37.0	33.0	37.0
6	35.52188180217232	37.0	35.0	37.0	33.0	37.0
7	35.74769868788879	37.0	35.0	37.0	35.0	37.0
8	35.594464350171336	37.0	35.0	37.0	35.0	37.0
9	37.25245600903805	39.0	37.0	39.0	34.0	39.0
10	36.859336740832646	39.0	37.0	39.0	32.0	39.0
11	36.82801451963479	39.0	37.0	39.0	33.0	39.0
12	36.66224348046209	39.0	35.0	39.0	33.0	39.0
13	36.588481173071486	39.0	35.0	39.0	32.0	39.0
14	37.64233289216062	40.0	36.0	41.0	33.0	41.0
15	37.79782205478146	40.0	36.0	41.0	33.0	41.0
16	37.85581152601487	40.0	36.0	41.0	33.0	41.0
17	37.793840562159254	40.0	36.0	41.0	33.0	41.0
18	37.75988068022895	39.0	36.0	41.0	33.0	41.0
19	37.70846192189837	39.0	36.0	41.0	33.0	41.0
20	37.650191183525244	39.0	36.0	41.0	33.0	41.0
21	37.49880227076338	39.0	35.0	41.0	33.0	41.0
22	37.48981109786378	39.0	35.0	41.0	33.0	41.0
23	37.433907691249935	39.0	35.0	41.0	32.0	41.0
24	37.4079826739442	39.0	35.0	41.0	32.0	41.0
25	37.285825229974954	39.0	35.0	41.0	32.0	41.0
26	37.22947023334235	39.0	35.0	41.0	32.0	41.0
27	37.15544228476423	39.0	35.0	41.0	32.0	41.0
28	37.038957061445934	39.0	35.0	41.0	32.0	41.0
29	36.99366055118984	39.0	35.0	41.0	31.0	41.0
30	36.8154215647516	39.0	35.0	41.0	31.0	41.0
31	36.73394784994711	39.0	35.0	41.0	31.0	41.0
32	36.61727824039277	39.0	35.0	40.0	31.0	41.0
33	36.51747997143576	39.0	35.0	40.0	30.0	41.0
34	36.43203726477082	39.0	35.0	40.0	30.0	41.0
35	36.358848429826196	39.0	35.0	40.0	30.0	41.0
36	36.26360200074692	38.0	35.0	40.0	30.0	41.0
37	36.198155825120594	38.0	35.0	40.0	30.0	41.0
38	36.054364250321505	38.0	35.0	40.0	30.0	41.0
39	36.00007812976103	38.0	35.0	40.0	30.0	41.0
40	35.85976645451832	38.0	35.0	40.0	29.0	41.0
41	35.77352057390997	38.0	35.0	40.0	29.0	41.0
42	35.686071494981725	38.0	35.0	40.0	29.0	41.0
43	35.56752208337696	38.0	34.0	40.0	28.0	41.0
44	35.41486121810548	38.0	34.0	40.0	28.0	41.0
45	35.34256618762706	38.0	34.0	40.0	28.0	41.0
46	35.177271739996655	37.0	34.0	40.0	27.0	41.0
47	35.100640507780945	37.0	34.0	40.0	27.0	41.0
48	35.02115285150189	37.0	34.0	40.0	27.0	41.0
49	34.93972288936357	37.0	34.0	40.0	26.0	41.0
50	34.82696914343218	37.0	34.0	40.0	26.0	41.0
51	33.24896204612469	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	6.0
12	13.0
13	10.0
14	19.0
15	48.0
16	96.0
17	152.0
18	299.0
19	570.0
20	914.0
21	1362.0
22	2004.0
23	2708.0
24	3802.0
25	4580.0
26	5810.0
27	7084.0
28	8532.0
29	10491.0
30	13052.0
31	16405.0
32	21399.0
33	29253.0
34	48718.0
35	60690.0
36	56697.0
37	78279.0
38	121510.0
39	145404.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99992968321507	18.249393322405584	22.823578311803374	13.92709868257597
2	29.287409701528688	25.444675534915405	26.616778209922167	18.651136553633737
3	32.56089042926053	25.237787927701845	24.856670953386224	17.3446506896514
4	28.79847365698847	27.27697469064521	24.357578039911807	19.566973612454508
5	24.792292030295595	31.466917515286084	23.990680682104067	19.75010977231425
6	24.716037383528057	35.51857066289977	24.025839074568605	15.739552879003565
7	80.62788201156008	6.74009822473557	7.729220999404651	4.9027987642997
8	81.59387837696359	5.451425946268601	7.183093969788784	5.771601706979019
9	75.54944754445974	7.762348018082352	10.105928330007611	6.582276107450298
10	40.68654183614314	25.58327773098673	16.94337623698944	16.786804195880688
11	32.58151668617306	27.072430976262616	21.398803989618116	18.947248347946203
12	27.16290523953803	23.70097552819625	28.05936611762279	21.076753114642923
13	24.747601806985113	26.216441314392597	29.02942523059999	20.006531648022303
14	20.548127151498292	29.511954634735556	27.809194622797328	22.130723590968827
15	20.640007750472293	25.350607302632504	33.52141771139179	20.48796723550341
16	22.05181253232619	25.722036186580123	30.430448105431424	21.79570317566227
17	21.708979140916398	24.603061749075334	27.45526680532095	26.232692304687315
18	24.86573400566597	23.401113505354232	28.51095613638956	23.222196352590235
19	24.96699017596385	27.38541879895806	25.015586887325945	22.632004137752144
20	25.582965211942604	26.376763584030904	25.272165022556063	22.768106181470433
21	24.831669429855882	28.336726769287502	26.053618892401254	20.77798490845536
22	23.042029123649723	24.920581097910656	25.230756249208934	26.806633529230687
23	21.596003506463678	28.44376454190177	24.499774204990615	25.46045774664394
24	22.56168735282306	24.931675523977244	28.364853483259132	24.14178363994056
25	22.375738521566156	26.899139166292947	26.372075798368964	24.353046513771933
26	21.429743374986913	27.7320024188974	25.703441303454426	25.134812902661256
27	22.976868902948773	27.812944851326876	24.856514693864156	24.35367155186019
28	21.199104320419526	27.69168746220473	27.248066679063253	23.86114153831249
29	23.07374980662884	26.476300899586068	25.338106540867333	25.111842752917756
30	24.30523110002016	27.046335636077824	24.96777147357417	23.680661790327846
31	25.86688876353403	27.922170257250052	21.871332784341547	24.339608194874376
32	25.714223210476888	26.975862591626676	22.79092007169187	24.518994126204564
33	24.819324927612776	27.286662781013217	21.901022093533825	25.992990197840182
34	23.25751100457684	26.79053879845803	24.894173238681734	25.057776958283394
35	23.169849412698586	25.731724276948125	25.34232554796308	25.75610076239021
36	24.034589607804225	29.053645456520005	23.16250521516155	23.749259720514218
37	24.139596006631653	27.181031344097526	24.24694629829005	24.432426350980762
38	23.762854298933842	28.38969874726741	24.27663560748233	23.570811346316418
39	23.923957866182473	24.640251515326717	24.343670942448057	27.09211967604276
40	23.640971871723433	23.89051832846064	27.7704422613253	24.698067538490626
41	20.531876161203574	25.122468400418153	25.671876879997374	28.673778558380903
42	23.84082780044409	24.33273277590353	25.70609771532953	26.120341708322854
43	24.399611851347192	23.950834503977585	26.076745301666822	25.5728083430084
44	24.338358118697858	24.377110480169886	25.431393475539917	25.85313792559234
45	23.97802366081683	24.31507544991023	25.61452963539966	26.09237125387328
46	23.916457409123367	25.512804686535585	25.79938465000211	24.771353254338937
47	22.478401027562615	24.8072929444138	27.40729513204711	25.30701089597647
48	22.007434828059836	24.171785468176967	27.139622570750404	26.681157133012796
49	23.73722773731524	23.33798465844012	27.621058158231516	25.30372944601312
50	22.227291975604764	24.017244800855053	27.773098673200398	25.98236455033979
51	21.666007772348628	24.37054758024317	25.209973732774344	28.753470914633862
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1353.0
1	1498.0
2	1643.0
3	1670.5
4	1698.0
5	1353.0
6	1008.0
7	1130.0
8	1252.0
9	1355.5
10	1459.0
11	1529.5
12	1600.0
13	1732.0
14	1864.0
15	1745.0
16	1626.0
17	1595.0
18	1564.0
19	1688.5
20	1813.0
21	1846.0
22	1879.0
23	2147.0
24	2415.0
25	2925.5
26	3598.5
27	3761.0
28	4534.0
29	5307.0
30	6580.5
31	7854.0
32	8874.5
33	9895.0
34	10811.0
35	11727.0
36	12177.5
37	12628.0
38	14636.0
39	16644.0
40	18970.0
41	21296.0
42	25606.5
43	29917.0
44	32996.0
45	36075.0
46	39756.0
47	43437.0
48	49640.0
49	55843.0
50	60242.5
51	64642.0
52	63303.0
53	61964.0
54	54775.0
55	47586.0
56	43080.0
57	38574.0
58	34586.5
59	30599.0
60	29039.5
61	27480.0
62	25091.0
63	22702.0
64	19934.0
65	17166.0
66	15553.5
67	13941.0
68	11742.0
69	9543.0
70	8553.5
71	7564.0
72	6791.0
73	6018.0
74	5279.5
75	4075.5
76	3610.0
77	2854.0
78	2098.0
79	1840.0
80	1582.0
81	1121.5
82	661.0
83	476.5
84	292.0
85	226.0
86	160.0
87	132.5
88	105.0
89	99.5
90	94.0
91	61.0
92	28.0
93	18.0
94	8.0
95	7.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639961.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.24383151750115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47604608748021	21.779649438719222
2	9.927069873797478	5.806111177035897
3	3.6729079949631678	3.222297077519575
4	1.8339875614061745	2.145312930038198
5	1.1778301475390174	1.7222133195432272
6	0.7757962810978404	1.3612353441797522
7	0.6130055694171435	1.254864211392736
8	0.5027652187077815	1.1762225078999982
9	0.4005700783332804	1.054278349355762
>10	5.742120689195711	40.94658739930066
>50	0.8124335905049919	14.58725908311543
>100	0.05902753960075655	3.111067723683821
>500	0.004292911970964114	0.9013857757800691
>1k	0.002146455985482057	0.9315156624356703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2196	0.3431459104539183	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1322	0.20657508816943532	TruSeq Adapter, Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1320	0.20626256912530608	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1098	0.17157295522695915	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	952	0.14875906500552377	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	831	0.12985166283570404	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	776	0.12125738912214963	TruSeq Adapter, Index 13 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	754	0.11781967963672786	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	736	0.11500700823956461	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.687785661938774E-4	0.0	0.0	0.2136067666623435	0.0
2	4.687785661938774E-4	0.0	0.0	1.0020923150004453	0.0
3	4.687785661938774E-4	0.0	0.0	1.3443006683219758	0.0
4	4.687785661938774E-4	0.0	0.0	1.8226110653617955	0.0
5	4.687785661938774E-4	0.0	0.0	3.1494106672125333	0.0
6	4.687785661938774E-4	0.0	0.0	4.253852969165308	0.0
7	4.687785661938774E-4	0.0	0.0	5.223443303576311	0.0
8	4.687785661938774E-4	0.0	0.0	6.994176207612651	0.0
9	4.687785661938774E-4	0.0	0.0	7.78391183212727	0.0
10	4.687785661938774E-4	0.0	0.0	9.192903942584001	0.0
11	4.687785661938774E-4	0.0	0.0	10.631585362233011	0.0
12	4.687785661938774E-4	0.0	0.0	11.793531168305568	0.0
13	4.687785661938774E-4	0.0	0.0	12.291374005603467	0.0
14	4.687785661938774E-4	0.0	0.0	12.500136727081806	0.0
15	4.687785661938774E-4	0.0	0.0	12.819218671137772	0.0
16	6.250380882585032E-4	0.0	0.0	13.49207217314805	0.0
17	6.250380882585032E-4	0.0	0.0	14.328998173326188	0.0
18	6.250380882585032E-4	0.0	0.0	15.406876356527976	0.0
19	6.250380882585032E-4	0.0	0.0	15.876123701288048	0.0
20	6.250380882585032E-4	0.0	0.0	16.385529743218726	0.0
21	6.250380882585032E-4	0.0	0.0	16.9338444061435	0.0
22	6.250380882585032E-4	0.0	0.0	17.470439604913423	0.0
23	6.250380882585032E-4	0.0	0.0	18.02656724394143	0.0
24	6.250380882585032E-4	0.0	0.0	18.465969019987156	0.0
25	6.250380882585032E-4	0.0	0.0	18.8350540111038	0.0
26	6.250380882585032E-4	0.0	0.0	19.19304457615386	0.0
27	6.250380882585032E-4	0.0	0.0	19.567129871976572	0.0
28	6.250380882585032E-4	0.0	0.0	19.96121638662356	0.0
29	6.250380882585032E-4	0.0	0.0	20.34295839902744	0.0
30	6.250380882585032E-4	0.0	0.0	20.811268186655123	0.0
31	6.250380882585032E-4	0.0	0.0	21.20051065611811	0.0
32	6.250380882585032E-4	0.0	0.0	21.589909385103155	0.0
33	6.250380882585032E-4	0.0	0.0	21.973370252249747	0.0
34	6.250380882585032E-4	0.0	0.0	22.355424783697757	0.0
35	6.250380882585032E-4	0.0	0.0	22.754667862572877	0.0
36	6.250380882585032E-4	0.0	0.0	23.127190563174942	0.0
37	9.375571323877548E-4	0.0	0.0	23.5081512779685	0.0
38	9.375571323877548E-4	0.0	0.0	23.886299321364895	0.0
39	9.375571323877548E-4	0.0	0.0	24.26241599097445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	25	3.8888596E-5	45.000004	12
ACGCATA	25	3.8888596E-5	45.000004	12
CGACAAT	35	1.2107921E-7	45.000004	20
CATAGAC	35	1.2107921E-7	45.000004	11
ACGTATA	25	3.8888596E-5	45.000004	11
CTCACGT	20	7.0309406E-4	45.0	45
GTACCGA	20	7.0309406E-4	45.0	9
ACGCATT	90	0.0	45.0	17
ACCGATC	20	7.0309406E-4	45.0	10
ATATGCG	45	3.8380676E-10	45.0	1
TCTAGCG	20	7.0309406E-4	45.0	1
ACTAGCA	20	7.0309406E-4	45.0	26
GAACCGA	20	7.0309406E-4	45.0	8
CCGCATT	20	7.0309406E-4	45.0	13
GCGATAT	30	2.163988E-6	44.999996	9
TCTACGC	105	0.0	42.857147	14
GTACGAG	60	3.6379788E-12	41.249996	1
TAAGGGT	160	0.0	40.78125	4
TACGCAT	105	0.0	40.714287	16
CTACGCA	105	0.0	40.714287	15
>>END_MODULE