FastQCFastQC Report
Sat 18 Jun 2016
SRR3553942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693838
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC24870.3584410193733984TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC17250.24861711235187467TruSeq Adapter, Index 14 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC12350.17799543985771896No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC11800.17006851743490556No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT10850.15637656052277332Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9620.13864907946811791No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA9300.13403705187666287No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8400.12106572427569547No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC8390.12092159841346252No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG8270.11919208806666687No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC8000.11530068978637664TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC7920.11414768288851287No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7890.11371530530181397No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7520.10838264839919405No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC7330.10564425701676759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG302.1642536E-645.0000048
TCTAGCC207.0313504E-445.00000435
CCGTACT207.0313504E-445.00000444
TATTACG302.1642536E-645.0000041
ACCACGT207.0313504E-445.00000412
CGTGTAC1600.045.00000418
TATAGCG406.8084773E-945.0000041
CTCGAAC207.0313504E-445.00000411
TATACGG650.045.0000042
TATAACG253.8892005E-545.0000041
CGTCAAA207.0313504E-445.00000426
TAGTACG502.1827873E-1145.0000041
TTTAGCG453.8380676E-1045.01
GTATACG351.2109922E-745.01
CGCTACA1800.043.7528
ACGCATT1900.042.6315817
TACGCAT1900.042.6315816
AGCTACG900.042.59
TCAAGCG750.042.017
ATAGCGG1250.041.42
Produced by FastQC (version 0.11.5)