##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693838
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.012154133962106	33.0	31.0	34.0	30.0	34.0
2	32.11388969759511	33.0	31.0	34.0	30.0	34.0
3	32.135750708378616	34.0	31.0	34.0	30.0	34.0
4	35.722962708874405	37.0	35.0	37.0	33.0	37.0
5	35.59656432769609	37.0	35.0	37.0	33.0	37.0
6	35.58412194200952	37.0	35.0	37.0	33.0	37.0
7	35.80178081915375	37.0	35.0	37.0	35.0	37.0
8	35.626564125919884	37.0	35.0	37.0	35.0	37.0
9	37.317669254206315	39.0	37.0	39.0	34.0	39.0
10	36.96394115052793	39.0	37.0	39.0	33.0	39.0
11	36.92718905565852	39.0	37.0	39.0	33.0	39.0
12	36.77850449240312	39.0	35.0	39.0	33.0	39.0
13	36.68968837106068	39.0	35.0	39.0	33.0	39.0
14	37.777024319797995	40.0	36.0	41.0	33.0	41.0
15	37.911417362554374	40.0	36.0	41.0	33.0	41.0
16	37.97919975556254	40.0	36.0	41.0	33.0	41.0
17	37.939382103603435	40.0	36.0	41.0	33.0	41.0
18	37.90130116828424	40.0	36.0	41.0	33.0	41.0
19	37.86003793392694	40.0	36.0	41.0	33.0	41.0
20	37.77141205872264	40.0	36.0	41.0	33.0	41.0
21	37.66026075250995	39.0	36.0	41.0	33.0	41.0
22	37.6477808940992	39.0	36.0	41.0	33.0	41.0
23	37.595445911005164	39.0	35.0	41.0	33.0	41.0
24	37.57512128191307	39.0	35.0	41.0	33.0	41.0
25	37.47930640870059	39.0	35.0	41.0	33.0	41.0
26	37.43190917764665	39.0	35.0	41.0	33.0	41.0
27	37.36185680230832	39.0	35.0	41.0	33.0	41.0
28	37.23430109045627	39.0	35.0	41.0	32.0	41.0
29	37.198351776639505	39.0	35.0	41.0	32.0	41.0
30	37.067707447559805	39.0	35.0	41.0	32.0	41.0
31	36.97911616256244	39.0	35.0	41.0	31.0	41.0
32	36.848448773344785	39.0	35.0	41.0	31.0	41.0
33	36.74412182670883	39.0	35.0	41.0	31.0	41.0
34	36.65552477667698	39.0	35.0	41.0	31.0	41.0
35	36.52622225937467	39.0	35.0	41.0	30.0	41.0
36	36.40430186873593	39.0	35.0	40.0	30.0	41.0
37	36.33941928807589	39.0	35.0	40.0	30.0	41.0
38	36.221153929303384	38.0	35.0	40.0	30.0	41.0
39	36.17466036740565	38.0	35.0	40.0	30.0	41.0
40	36.03015257163776	38.0	35.0	40.0	29.0	41.0
41	35.8747517432023	38.0	35.0	40.0	29.0	41.0
42	35.748795828421045	38.0	35.0	40.0	29.0	41.0
43	35.640730256918765	38.0	34.0	40.0	28.0	41.0
44	35.523133642147016	38.0	34.0	40.0	28.0	41.0
45	35.43898287496505	37.0	34.0	40.0	28.0	41.0
46	35.23362369890378	37.0	34.0	40.0	28.0	41.0
47	35.06057898241376	37.0	34.0	40.0	27.0	41.0
48	34.8895967070123	36.0	34.0	40.0	27.0	41.0
49	34.77959840769748	36.0	34.0	40.0	27.0	41.0
50	34.6116874544202	36.0	33.0	40.0	26.0	41.0
51	33.047241575122726	35.0	31.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	8.0
12	6.0
13	9.0
14	19.0
15	48.0
16	63.0
17	155.0
18	259.0
19	472.0
20	852.0
21	1389.0
22	1843.0
23	2541.0
24	3423.0
25	4575.0
26	5948.0
27	7153.0
28	8773.0
29	10906.0
30	13615.0
31	17338.0
32	22450.0
33	30583.0
34	51519.0
35	66589.0
36	61312.0
37	87924.0
38	135072.0
39	158928.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68272997443207	18.638356503967785	24.17106010336707	14.507853418233074
2	29.557043575013186	27.039885391114353	25.92175695191096	17.481314081961496
3	32.02217232264592	25.44815936861342	25.94236695021028	16.587301358530375
4	29.287672338499764	26.99664763244446	24.950348640460742	18.765331388595033
5	25.0721349940476	30.288626451707746	24.273677717276943	20.36556083696771
6	24.882465359349013	35.64708188366737	23.840002997817937	15.630449759165685
7	80.60339733482455	6.412447862469338	8.07897520746918	4.905179595236929
8	81.78883255169073	5.810866513508917	7.068220535629354	5.332080399170988
9	75.64503529642366	8.056347447098602	9.408824538292802	6.889792718184936
10	42.13807834105368	24.349920298398185	16.790230572554403	16.72177078799374
11	32.58498381466567	25.54573257734514	21.476050605472746	20.393233002516435
12	27.65069079525768	22.55656219463333	29.243281572932013	20.549465437176977
13	24.945160109420357	25.007854859491697	29.54666651293241	20.500318518155535
14	20.95618862039842	28.746767977539427	28.570646173890736	21.726397228171418
15	20.17848546778931	25.18167064934466	33.30446011893266	21.33538376393337
16	22.1839968407611	24.037455429077102	30.667821595242696	23.1107261349191
17	22.112942790680247	24.18965233959512	27.081681891161914	26.615722978562715
18	23.636353154482748	24.430342529524182	28.6653368653778	23.267967450615274
19	25.19046232694087	25.978542541630755	25.64590005159706	23.185095079831314
20	25.09577163545381	25.780513606922657	25.61520124294144	23.508513514682104
21	24.936368431824143	26.914063513384967	26.680147238980854	21.469420815810032
22	23.28396542132313	24.712973345363036	25.87390716564962	26.129154067664214
23	21.516549972760213	26.976325885869613	26.257426085051556	25.24969805631862
24	22.377413747877746	25.547173835967474	27.908531962792466	24.166880453362314
25	22.562615480847114	26.510655224994885	26.611543328557964	24.31518596560004
26	21.775400021330626	27.62705415385148	25.59545599981552	25.002089825002376
27	23.82040188055425	26.72194373902842	25.98603708646687	23.471617293950462
28	22.376981370291045	26.553316480215845	27.87696839896345	23.192733750529662
29	24.311582819044215	25.50090943419069	26.026536453754336	24.16097129301076
30	24.94285409562463	26.434124392149176	25.129641213078557	23.49338029914764
31	28.12054110613717	26.468714599085093	22.38433178926493	23.026412505512813
32	27.44790570709589	26.970272599655825	23.062876348657756	22.518945344590524
33	24.96202283530161	27.396164522554255	22.59778219123196	25.044030450912175
34	25.941358069174647	24.944583605971424	24.556740910702498	24.55731741415143
35	25.806744513849054	25.05599289747751	24.752752083339338	24.3845105053341
36	26.83450603743237	27.353503267333295	23.76649880807912	22.045491887155215
37	25.911668141554657	27.130540558458893	25.535932018713304	21.42185928127315
38	24.777829983367873	27.274378168967395	24.421839103652438	23.52595274401229
39	25.974651143350464	25.063487442313626	24.479921826132326	24.481939588203584
40	25.97320988472814	23.176015150510636	27.265442365508953	23.585332599252276
41	22.74147567587823	25.664780539549575	25.8528647897636	25.740878994808586
42	23.759004263243007	25.94279932779698	26.283801117840188	24.01439529111983
43	24.124363324003586	24.73199795917779	26.93741190306671	24.206226813751915
44	24.716720617781096	23.98744375488226	25.741743749981982	25.554091877354658
45	25.34510937711685	23.775434611537563	25.207180926959893	25.672275084385692
46	25.094762754418177	24.831012426531842	26.188678048766427	23.885546770283554
47	24.300773379376743	24.386240015680894	28.230509139020928	23.08247746592144
48	22.315583752979805	25.06723471473168	27.390831865651638	25.22634966663688
49	23.652351125190606	24.22986345515812	28.42219653579078	23.695588883860495
50	23.200948924676943	23.5893681233948	28.212637532104033	24.997045419824225
51	22.233287885644774	24.042932211841958	26.051181976196176	27.672597926317096
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1341.0
1	1537.0
2	1733.0
3	1744.0
4	1755.0
5	1377.5
6	1000.0
7	1022.5
8	1045.0
9	1203.0
10	1361.0
11	1390.5
12	1420.0
13	1459.0
14	1498.0
15	1455.5
16	1413.0
17	1433.0
18	1453.0
19	1618.0
20	1783.0
21	1714.5
22	1646.0
23	1907.5
24	2169.0
25	2985.5
26	4351.5
27	4901.0
28	5867.0
29	6833.0
30	7961.0
31	9089.0
32	10251.5
33	11414.0
34	11731.5
35	12049.0
36	13434.0
37	14819.0
38	16174.5
39	17530.0
40	21481.5
41	25433.0
42	28014.0
43	30595.0
44	35707.0
45	40819.0
46	43238.5
47	45658.0
48	53517.5
49	61377.0
50	64114.5
51	66852.0
52	66250.5
53	65649.0
54	59016.5
55	52384.0
56	46734.5
57	41085.0
58	38012.5
59	34940.0
60	33250.5
61	31561.0
62	28412.5
63	25264.0
64	22585.0
65	19906.0
66	17143.0
67	14380.0
68	12505.0
69	10630.0
70	9617.5
71	8605.0
72	7637.0
73	6669.0
74	5957.0
75	3989.5
76	2734.0
77	2337.5
78	1941.0
79	1613.0
80	1285.0
81	866.0
82	447.0
83	309.0
84	171.0
85	124.0
86	77.0
87	63.0
88	49.0
89	34.0
90	19.0
91	12.5
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	693838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.235981899648927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8594649915128	20.116350516265957
2	10.23457365620948	5.5749732570229025
3	3.8169541741809825	3.118754843993479
4	1.8899653989196321	2.0590025358375144
5	1.1470728094162674	1.562082713742045
6	0.8040912578508065	1.3140128966694302
7	0.593601967891103	1.1317132717154643
8	0.4566645257916048	0.9950165406937526
9	0.39285927255424646	0.962991723275703
>10	5.6783409513293295	39.10875966167844
>50	1.0457255696782406	17.896994350849464
>100	0.07166139690687875	3.673806635830078
>500	0.006369901947278111	1.37057521969498
>1k	0.0026541258113658797	1.1149658327307685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	2487	0.3584410193733984	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1725	0.24861711235187467	TruSeq Adapter, Index 14 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1235	0.17799543985771896	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1180	0.17006851743490556	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	1085	0.15637656052277332	Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	962	0.13864907946811791	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	930	0.13403705187666287	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	840	0.12106572427569547	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	839	0.12092159841346252	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	827	0.11919208806666687	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	800	0.11530068978637664	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	792	0.11414768288851287	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	789	0.11371530530181397	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	752	0.10838264839919405	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	733	0.10564425701676759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.882517244659416E-4	0.0	0.0	0.16170921742539324	0.0
2	2.882517244659416E-4	0.0	0.0	0.9953332045808964	0.0
3	2.882517244659416E-4	0.0	0.0	1.3550713567143915	0.0
4	2.882517244659416E-4	0.0	0.0	1.8396225055416393	0.0
5	2.882517244659416E-4	0.0	0.0	3.2607035071587314	0.0
6	2.882517244659416E-4	0.0	0.0	4.285150135910688	0.0
7	2.882517244659416E-4	0.0	0.0	5.197322717983161	0.0
8	2.882517244659416E-4	0.0	0.0	6.566518409196383	0.0
9	2.882517244659416E-4	0.0	0.0	7.113764308094973	0.0
10	2.882517244659416E-4	0.0	0.0	8.309864838766398	0.0
11	2.882517244659416E-4	0.0	0.0	9.781966395613962	0.0
12	2.882517244659416E-4	0.0	0.0	11.030384614275954	0.0
13	2.882517244659416E-4	0.0	0.0	11.49346100963049	0.0
14	2.882517244659416E-4	0.0	0.0	11.680968756395584	0.0
15	2.882517244659416E-4	0.0	0.0	12.015340756776077	0.0
16	2.882517244659416E-4	0.0	0.0	12.718530838610741	0.0
17	2.882517244659416E-4	0.0	0.0	13.538462868854113	0.0
18	2.882517244659416E-4	0.0	0.0	14.509006425130938	0.0
19	2.882517244659416E-4	0.0	0.0	14.990963308437992	0.0
20	2.882517244659416E-4	0.0	0.0	15.479982358994462	0.0
21	2.882517244659416E-4	0.0	0.0	16.043658606187613	0.0
22	2.882517244659416E-4	0.0	0.0	16.586724855081446	0.0
23	2.882517244659416E-4	0.0	0.0	17.14636557813207	0.0
24	2.882517244659416E-4	0.0	0.0	17.58638183552933	0.0
25	2.882517244659416E-4	0.0	0.0	17.992096137715144	0.0
26	2.882517244659416E-4	0.0	0.0	18.35918470882252	0.0
27	2.882517244659416E-4	0.0	0.0	18.729588174761254	0.0
28	2.882517244659416E-4	0.0	0.0	19.125934295901924	0.0
29	2.882517244659416E-4	0.0	0.0	19.502391048054445	0.0
30	2.882517244659416E-4	0.0	0.0	19.942695557176172	0.0
31	2.882517244659416E-4	0.0	0.0	20.323187833471213	0.0
32	2.882517244659416E-4	0.0	0.0	20.697338571828006	0.0
33	2.882517244659416E-4	0.0	0.0	21.07797497398528	0.0
34	2.882517244659416E-4	0.0	0.0	21.47417696926372	0.0
35	2.882517244659416E-4	0.0	0.0	21.878161761102735	0.0
36	2.882517244659416E-4	0.0	0.0	22.243809073587784	0.0
37	2.882517244659416E-4	0.0	0.0	22.616374427460013	0.0
38	2.882517244659416E-4	0.0	0.0	22.99038103995457	0.0
39	2.882517244659416E-4	0.0	0.0	23.360928631755538	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATACG	30	2.1642536E-6	45.000004	8
TCTAGCC	20	7.0313504E-4	45.000004	35
CCGTACT	20	7.0313504E-4	45.000004	44
TATTACG	30	2.1642536E-6	45.000004	1
ACCACGT	20	7.0313504E-4	45.000004	12
CGTGTAC	160	0.0	45.000004	18
TATAGCG	40	6.8084773E-9	45.000004	1
CTCGAAC	20	7.0313504E-4	45.000004	11
TATACGG	65	0.0	45.000004	2
TATAACG	25	3.8892005E-5	45.000004	1
CGTCAAA	20	7.0313504E-4	45.000004	26
TAGTACG	50	2.1827873E-11	45.000004	1
TTTAGCG	45	3.8380676E-10	45.0	1
GTATACG	35	1.2109922E-7	45.0	1
CGCTACA	180	0.0	43.75	28
ACGCATT	190	0.0	42.63158	17
TACGCAT	190	0.0	42.63158	16
AGCTACG	90	0.0	42.5	9
TCAAGCG	75	0.0	42.0	17
ATAGCGG	125	0.0	41.4	2
>>END_MODULE