FastQCFastQC Report
Sat 18 Jun 2016
SRR3553941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG65680.6846066690779387No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC62080.6470825520152015No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC51590.5377414442407256No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC31430.32760638868939723No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT15000.15635048776140498No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC14310.14915836532438034No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT13840.14425938337452301No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT13710.14290434581392417No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC12030.1253930911846468No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT11600.12091104386881986No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC11580.12070257655180465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT207.0327026E-445.019
CCGTCGA351.211647E-745.041
ATATCGT207.0327026E-445.02
TCGCTAA207.0327026E-445.045
AACGCAC207.0327026E-445.042
ACTACGC207.0327026E-445.028
TCGAGTA207.0327026E-445.017
TATACGC253.8903214E-545.045
CCGCATT253.8903214E-545.029
TAACACG302.165125E-644.99999626
GCTACGA1100.042.95454810
CTAACGG800.042.18752
GTAAGCG556.002665E-1140.9090921
CGACTAA900.040.017
GTAATCG403.457426E-739.3751
TACGATG403.457426E-739.37529
CGTCGAA403.457426E-739.37542
TTAACGG1200.039.3749962
GGACCGT750.039.0000048
TAAGGGT2950.038.8983044