##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695187
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0402395326725	33.0	31.0	34.0	30.0	34.0
2	32.11899244375974	33.0	31.0	34.0	30.0	34.0
3	32.120417959484286	33.0	31.0	34.0	30.0	34.0
4	35.77667160059092	37.0	35.0	37.0	35.0	37.0
5	35.68329384755469	37.0	35.0	37.0	33.0	37.0
6	35.65106367063826	37.0	35.0	37.0	33.0	37.0
7	35.93314173021072	37.0	35.0	37.0	35.0	37.0
8	35.87708774761323	37.0	35.0	37.0	35.0	37.0
9	37.610297660917134	39.0	38.0	39.0	35.0	39.0
10	37.066920123650185	39.0	37.0	39.0	33.0	39.0
11	36.96408448374322	39.0	37.0	39.0	33.0	39.0
12	36.84146999296592	39.0	35.0	39.0	33.0	39.0
13	36.76001565046527	39.0	35.0	39.0	33.0	39.0
14	37.86019445127714	40.0	37.0	41.0	33.0	41.0
15	38.052411797113585	40.0	37.0	41.0	33.0	41.0
16	38.08648176677642	40.0	37.0	41.0	33.0	41.0
17	38.02606924467805	40.0	36.0	41.0	33.0	41.0
18	37.99105420555908	40.0	36.0	41.0	33.0	41.0
19	37.9170036263624	40.0	36.0	41.0	33.0	41.0
20	37.834523660540256	40.0	36.0	41.0	33.0	41.0
21	37.71177826973174	40.0	35.0	41.0	33.0	41.0
22	37.6875488178001	40.0	35.0	41.0	33.0	41.0
23	37.61016100703839	40.0	35.0	41.0	33.0	41.0
24	37.557060186683586	39.0	35.0	41.0	33.0	41.0
25	37.39059130852562	39.0	35.0	41.0	33.0	41.0
26	37.33768611898669	39.0	35.0	41.0	33.0	41.0
27	37.2994259098631	39.0	35.0	41.0	32.0	41.0
28	37.224027491883476	39.0	35.0	41.0	32.0	41.0
29	37.167698763066625	39.0	35.0	41.0	32.0	41.0
30	37.012764910736244	39.0	35.0	41.0	32.0	41.0
31	36.92905649846732	39.0	35.0	41.0	31.0	41.0
32	36.77590633886997	39.0	35.0	41.0	31.0	41.0
33	36.684041847733056	39.0	35.0	41.0	31.0	41.0
34	36.53052056497029	39.0	35.0	41.0	31.0	41.0
35	36.43939256631669	39.0	35.0	41.0	30.0	41.0
36	36.259117331020285	39.0	35.0	41.0	30.0	41.0
37	36.214405620358264	39.0	35.0	41.0	30.0	41.0
38	36.03904992469652	38.0	35.0	41.0	30.0	41.0
39	35.95617294339509	38.0	35.0	40.0	29.0	41.0
40	35.773496915218495	38.0	35.0	40.0	29.0	41.0
41	35.654662702265725	38.0	35.0	40.0	28.0	41.0
42	35.5162366384872	38.0	34.0	40.0	28.0	41.0
43	35.44443581367315	38.0	34.0	40.0	28.0	41.0
44	35.309539735351784	38.0	34.0	40.0	27.0	41.0
45	35.23785974133578	37.0	34.0	40.0	27.0	41.0
46	35.115791866073444	37.0	34.0	40.0	27.0	41.0
47	34.99012352072176	37.0	34.0	40.0	26.0	41.0
48	34.87665477058691	37.0	34.0	40.0	26.0	41.0
49	34.769338321919136	37.0	34.0	40.0	26.0	41.0
50	34.63329291255447	36.0	34.0	40.0	26.0	41.0
51	33.13674162491531	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	3.0
11	6.0
12	8.0
13	15.0
14	25.0
15	51.0
16	89.0
17	188.0
18	321.0
19	599.0
20	944.0
21	1511.0
22	2101.0
23	2928.0
24	4042.0
25	5377.0
26	7019.0
27	8312.0
28	9506.0
29	11003.0
30	13439.0
31	16747.0
32	21060.0
33	29653.0
34	50879.0
35	63983.0
36	59017.0
37	82346.0
38	129029.0
39	174929.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3951052019097	18.285008206425037	23.172470141127498	14.14741645053777
2	30.191013353241647	25.208325241985253	26.209926250059333	18.390735154713767
3	34.00336887772642	23.92938878316194	25.87951155588353	16.187730783228112
4	28.113442857820992	26.923691035649398	25.133956762712767	19.828909343816843
5	24.623302794787588	31.266982840588213	24.496574303029256	19.61314006159494
6	25.40899067445162	37.14971655108626	23.277765550851782	14.163527223610336
7	83.66871072100025	6.5199723239934	6.2610491853271135	3.5502677696792375
8	86.35273674565262	5.052309666319997	5.670992121544275	2.923961466483119
9	81.24648475877714	7.75143954072789	6.8690870226284435	4.132988677866531
10	44.662802958053014	25.47458453624708	14.625992718505957	15.23661978719395
11	33.91260193300508	27.551579646915147	19.70865393052517	18.827164489554608
12	29.863331736640646	23.804242599473234	26.846445632613957	19.485980031272163
13	24.881506702513136	25.608793029789105	28.16004902278092	21.349651244916835
14	21.76277749727771	29.320887761134774	27.049844142655143	21.866490598932373
15	21.69243671127337	26.865721021825784	29.876709432138405	21.565132834762444
16	24.638838183107566	24.08977728294689	30.53164112677596	20.73974340716958
17	23.962041867871523	24.478018144758174	27.063941069093637	24.495998918276666
18	24.535125081452904	26.108802379791335	26.293788577749584	23.062283961006173
19	26.43504553451086	27.077462610779545	24.669621267371227	21.81787058733837
20	27.9943382140345	25.899362329847943	24.502328150555176	21.60397130556239
21	27.83783356132954	26.66419251223052	24.11926575151722	21.37870817492272
22	25.38885220811091	25.330594501910998	23.878898771122014	25.401654518856077
23	25.36195297092725	27.789213549735535	24.487511993175936	22.36132148616128
24	24.089345744382445	27.254537268389655	26.32457166201324	22.331545325214655
25	25.267877563878493	26.67555636109421	25.27550141185034	22.78106466317696
26	23.41067942870048	28.1128674730684	24.452125830891543	24.02432726733958
27	23.900044160779764	27.043083371812187	26.162888546534962	22.89398392087309
28	22.552636916398033	28.98155460329379	26.86989256128207	21.595915919026105
29	23.60933101453278	27.323439592512518	26.049106211709944	23.01812318124476
30	24.6782520386601	26.749205609425953	25.86800386083169	22.704538491082253
31	26.780564078442204	28.2800167436963	22.77948235510733	22.159936822754165
32	25.76112614303777	28.283037513647407	23.395575578944946	22.560260764369875
33	25.648782270094234	27.85682125816507	22.927356236523412	23.567040235217288
34	24.10099728562243	28.810377639397743	23.984769565598896	23.10385550938093
35	25.022619813086262	26.242291642392622	24.34999503730651	24.385093507214606
36	25.493140694518168	29.654754763826137	23.096663199973534	21.755441341682165
37	25.509539159967034	28.717740694230475	24.095674976660955	21.67704516914154
38	24.35948888572427	29.30319467999258	23.788994903529552	22.548321530753597
39	25.021756735957375	27.077462610779545	24.249878090355544	23.650902562907532
40	25.487674539368545	27.122630313857996	26.462088617882674	20.927606528890788
41	23.865089537059813	26.831485629046576	25.668633044058648	23.634791789834967
42	23.78568644120215	27.133850316533536	25.2081813957971	23.872281846467207
43	24.47686737525299	26.439936304907892	25.14690291964608	23.936293400193044
44	23.933416476430082	26.60766096028838	25.72286305699042	23.736059506291113
45	24.63581741315646	26.99777182254559	24.675662807273437	23.690747957024513
46	24.814905917400644	27.553161954984773	24.19305884603711	23.438873281577475
47	24.964793645450793	27.599048889003964	25.261548331599986	22.174609133945257
48	24.87143746934278	26.940521039662706	25.160568307520137	23.027473183474374
49	24.34078888126504	27.784466625526655	25.428985294604185	22.44575919860412
50	23.050488573578047	27.687514294714948	26.03616005477663	23.225837076930382
51	23.70987950004819	27.756704311214104	24.51599353842923	24.01742265030848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2110.0
1	2455.5
2	2801.0
3	3023.0
4	3245.0
5	2485.0
6	1725.0
7	1755.5
8	1786.0
9	1875.5
10	1965.0
11	2005.0
12	2045.0
13	2113.5
14	2182.0
15	2182.0
16	2182.0
17	2180.5
18	2179.0
19	2106.0
20	2033.0
21	2295.0
22	2557.0
23	2636.0
24	2715.0
25	3547.5
26	5067.5
27	5755.0
28	6831.5
29	7908.0
30	9186.5
31	10465.0
32	12239.0
33	14013.0
34	15353.0
35	16693.0
36	18159.5
37	19626.0
38	20349.5
39	21073.0
40	23870.5
41	26668.0
42	30405.5
43	34143.0
44	37404.5
45	40666.0
46	41804.0
47	42942.0
48	45649.5
49	48357.0
50	49532.0
51	50707.0
52	46986.0
53	43265.0
54	41790.0
55	40315.0
56	37954.0
57	35593.0
58	35724.5
59	35856.0
60	34746.0
61	33636.0
62	31911.0
63	30186.0
64	27188.0
65	24190.0
66	21853.5
67	19517.0
68	18190.5
69	16864.0
70	15130.0
71	13396.0
72	11730.0
73	10064.0
74	9081.0
75	6633.0
76	5168.0
77	4156.5
78	3145.0
79	2303.0
80	1461.0
81	1114.5
82	768.0
83	605.0
84	442.0
85	284.0
86	126.0
87	89.5
88	53.0
89	48.0
90	43.0
91	50.0
92	57.0
93	35.5
94	14.0
95	9.5
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.52288539320774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48942531501847	16.247502494542577
2	7.825747518347034	3.368653339071262
3	2.7905278537480522	1.801806335483199
4	1.4952666805685606	1.287298135926372
5	0.9956685008684012	1.071482951690878
6	0.7626004988306091	0.9848017882280553
7	0.5902428789461303	0.8892610886400185
8	0.4867847396764798	0.8381609730575471
9	0.4409016035580341	0.8540527214754974
>10	6.997614958838564	38.282323595468085
>50	1.9642179779936757	28.099583073330802
>100	0.15228056045237853	4.643245410499829
>500	0.006708394733584957	1.0245489422216731
>1k	0.0020125184200754874	0.6072791503641992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1697	0.24410698128704938	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1403	0.20181620197155586	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1106	0.1590938840916185	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	887	0.12759156888722026	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	885	0.1273038765109244	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	799	0.11493310433020179	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	796	0.114501565765758	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	721	0.10371310165466271	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.051065396792517696	0.0
2	0.0	0.0	0.0	0.22972236247225566	0.0
3	0.0	0.0	0.0	0.30639238075510616	0.0
4	0.0	0.0	0.0	0.4063654815179225	0.0
5	0.0	0.0	0.0	0.7251286344537513	0.0
6	0.0	0.0	0.0	0.9980048533703881	0.0
7	0.0	0.0	0.0	1.2052872104915655	0.0
8	0.0	0.0	0.0	1.5594365257117868	0.0
9	0.0	0.0	0.0	1.7088927151974935	0.0
10	0.0	0.0	0.0	2.0702343398251117	0.0
11	0.0	0.0	0.0	2.5770044606702944	0.0
12	0.0	0.0	0.0	2.923529927918675	0.0
13	0.0	0.0	0.0	3.049539188736268	0.0
14	0.0	0.0	0.0	3.104919971173224	0.0
15	0.0	0.0	0.0	3.214818458918248	0.0
16	0.0	0.0	0.0	3.4567677473830782	0.0
17	0.0	0.0	0.0	3.745898585560432	0.0
18	0.0	0.0	0.0	4.0881086671643745	0.0
19	0.0	0.0	0.0	4.255545630168573	0.0
20	1.4384618814793716E-4	0.0	0.0	4.4162218223298195	0.0
21	1.4384618814793716E-4	0.0	0.0	4.618757255242115	0.0
22	1.4384618814793716E-4	0.0	0.0	4.838122692167719	0.0
23	1.4384618814793716E-4	0.0	0.0	5.081798134890325	0.0
24	1.4384618814793716E-4	0.0	0.0	5.27944279740559	0.0
25	1.4384618814793716E-4	0.0	0.0	5.453928223629038	0.0
26	2.876923762958743E-4	0.0	0.0	5.611008261086585	0.0
27	4.3153856444381154E-4	0.0	0.0	5.774849069387086	0.0
28	5.753847525917487E-4	0.0	0.0	5.934230645855	0.0
29	5.753847525917487E-4	0.0	0.0	6.113894534851774	0.0
30	5.753847525917487E-4	0.0	0.0	6.33656843410478	0.0
31	5.753847525917487E-4	0.0	0.0	6.524719248202282	0.0
32	5.753847525917487E-4	0.0	0.0	6.718623909825702	0.0
33	7.192309407396859E-4	0.0	0.0	6.902603184466913	0.0
34	8.630771288876231E-4	0.0	0.0	7.09391861470367	0.0
35	8.630771288876231E-4	0.0	0.0	7.315154052075197	0.0
36	8.630771288876231E-4	0.0	0.0	7.515387945977126	0.0
37	0.0010069233170355602	0.0	0.0	7.717347994136829	0.0
38	0.0010069233170355602	0.0	0.0	7.918876503732089	0.0
39	0.0010069233170355602	0.0	0.0	8.125439629912528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	25	3.889209E-5	45.000004	24
GATCGGA	20	7.03136E-4	45.000004	8
CACGCGT	30	2.164259E-6	45.000004	42
CACGATT	20	7.03136E-4	45.000004	24
AAGCGAT	20	7.03136E-4	45.000004	23
GCGCATT	25	3.889209E-5	45.000004	23
TTATCCG	35	1.2109922E-7	45.000004	1
ATCGTGT	20	7.03136E-4	45.000004	10
AATGCGT	20	7.03136E-4	45.000004	31
GTTCGCA	20	7.03136E-4	45.000004	17
CGTAGCA	20	7.03136E-4	45.000004	29
CCGACCG	35	1.2109922E-7	45.000004	15
CAATGCG	25	3.889209E-5	45.000004	1
TCGAGTA	20	7.03136E-4	45.000004	38
CAGTACG	20	7.03136E-4	45.000004	1
CGAATAT	65	0.0	44.999996	14
TATTAGG	85	0.0	42.35294	2
ACGCATT	225	0.0	42.0	17
CGCATGG	70	0.0	41.785717	2
GCGTTAG	70	0.0	41.785717	1
>>END_MODULE