FastQCFastQC Report
Sat 18 Jun 2016
SRR3553937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382825
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18080.47227845621367465No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC11560.3019656501012212TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC9230.24110233135244563TruSeq Adapter, Index 13 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9210.24057989943185532No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.21889897472735584No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT7490.19565075426108536TruSeq Adapter, Index 13 (100% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC5610.14654215372559262No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG4410.11519623849017176No Hit
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC4340.11336772676810553TruSeq Adapter, Index 13 (96% over 25bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC4280.1118004310063345No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC4240.11075556716515378TruSeq Adapter, Index 13 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC4160.10866583948279239TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTA302.1616943E-645.0000046
CACCGTA302.1616943E-645.00000425
GTAGTGC302.1616943E-645.00000429
TAATACG207.027385E-445.01
CTGTCGA207.027385E-445.012
ACAGTCG207.027385E-445.01
CTCGTCA253.8859143E-545.036
GTCGAGT207.027385E-445.014
CGAGTAC253.8859143E-545.017
GCGAATG207.027385E-445.01
TATGGTC502.1827873E-1145.043
ATAATGC207.027385E-445.033
GACCGAA207.027385E-445.09
ACCGAAC207.027385E-445.010
CGATTAC207.027385E-445.013
TAGCACG207.027385E-445.01
CGGATAT351.2090823E-745.06
TACGAGC207.027385E-445.04
TATTAGT453.8380676E-1045.030
TTGCGAG207.027385E-445.01
Produced by FastQC (version 0.11.5)