##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382825
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81187487755502	31.0	31.0	34.0	30.0	34.0
2	31.888604453732125	33.0	31.0	34.0	30.0	34.0
3	31.895416965976622	33.0	31.0	34.0	30.0	34.0
4	35.580871155227584	37.0	35.0	37.0	33.0	37.0
5	35.50000391823941	37.0	35.0	37.0	33.0	37.0
6	35.46622085809443	37.0	35.0	37.0	33.0	37.0
7	35.76236139228107	37.0	35.0	37.0	35.0	37.0
8	35.68302749297982	37.0	35.0	37.0	35.0	37.0
9	37.376830144321815	39.0	37.0	39.0	35.0	39.0
10	36.83408084633971	39.0	37.0	39.0	32.0	39.0
11	36.771391628028475	39.0	37.0	39.0	32.0	39.0
12	36.716886305753285	39.0	35.0	39.0	33.0	39.0
13	36.67206425912623	39.0	35.0	39.0	33.0	39.0
14	37.74005354927186	40.0	36.0	41.0	33.0	41.0
15	37.898914647684975	40.0	37.0	41.0	33.0	41.0
16	37.93507738522824	40.0	36.0	41.0	33.0	41.0
17	37.850730751648925	40.0	36.0	41.0	33.0	41.0
18	37.81096062169399	39.0	36.0	41.0	33.0	41.0
19	37.72529484751518	39.0	36.0	41.0	33.0	41.0
20	37.65499379612094	39.0	36.0	41.0	33.0	41.0
21	37.52479070071181	39.0	35.0	41.0	33.0	41.0
22	37.51995559328675	39.0	35.0	41.0	33.0	41.0
23	37.45526807287925	39.0	35.0	41.0	33.0	41.0
24	37.40167178214589	39.0	35.0	41.0	33.0	41.0
25	37.28471756024293	39.0	35.0	41.0	32.0	41.0
26	37.25278390909685	39.0	35.0	41.0	32.0	41.0
27	37.23189969307125	39.0	35.0	41.0	32.0	41.0
28	37.10692875334683	39.0	35.0	41.0	32.0	41.0
29	37.05405080650428	39.0	35.0	41.0	32.0	41.0
30	36.861038333442174	39.0	35.0	41.0	31.0	41.0
31	36.802609547443346	39.0	35.0	41.0	31.0	41.0
32	36.651067720237705	39.0	35.0	41.0	31.0	41.0
33	36.52541761901652	39.0	35.0	41.0	30.0	41.0
34	36.41779664337491	39.0	35.0	41.0	30.0	41.0
35	36.322959576830144	39.0	35.0	40.0	30.0	41.0
36	36.182934761313916	39.0	35.0	40.0	30.0	41.0
37	36.15560112322863	38.0	35.0	40.0	30.0	41.0
38	36.03826552602364	38.0	35.0	40.0	30.0	41.0
39	35.95762815908052	38.0	35.0	40.0	29.0	41.0
40	35.832800888134265	38.0	35.0	40.0	29.0	41.0
41	35.708676288121204	38.0	35.0	40.0	28.0	41.0
42	35.598511069026316	38.0	35.0	40.0	28.0	41.0
43	35.49016391301509	38.0	34.0	40.0	27.0	41.0
44	35.377214131783454	38.0	34.0	40.0	27.0	41.0
45	35.302864233004634	38.0	34.0	40.0	27.0	41.0
46	35.18121334813557	38.0	34.0	40.0	26.0	41.0
47	35.08118853261934	37.0	34.0	40.0	26.0	41.0
48	34.993905831646316	37.0	34.0	40.0	26.0	41.0
49	34.928844772415594	37.0	34.0	40.0	26.0	41.0
50	34.79416443544701	37.0	34.0	40.0	26.0	41.0
51	33.33214131783452	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	2.0
12	3.0
13	5.0
14	12.0
15	35.0
16	48.0
17	94.0
18	190.0
19	325.0
20	646.0
21	918.0
22	1407.0
23	1796.0
24	2460.0
25	3158.0
26	3759.0
27	4523.0
28	5218.0
29	6289.0
30	7570.0
31	9594.0
32	12296.0
33	16742.0
34	27853.0
35	34604.0
36	33235.0
37	47043.0
38	73400.0
39	89561.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99784496832756	19.19754456997323	21.62058381767126	14.18402664402795
2	30.267876967282703	26.19891595376477	24.344805067589633	19.188402011362896
3	31.923986155554108	25.936393913668127	25.667602690524394	16.47201724025338
4	27.43290015019918	27.77143603474172	25.132109971919288	19.663553843139816
5	23.050218768366747	32.363873832691176	24.398093123489843	20.18781427545223
6	24.08071573173121	36.86175145301378	24.28054594135702	14.776986873897997
7	79.3668125122445	7.803304381897734	8.179455364722784	4.650427741134983
8	80.82518121857245	6.7738522823744525	7.627244824658787	4.773721674394305
9	74.70175667733298	8.783125448964931	10.074577156664272	6.440540717037811
10	39.632207927904396	26.184810291908832	17.715927643178997	16.46705413700777
11	29.883105857767912	27.59067459021746	23.438646901325672	19.08757265068896
12	26.14667276170574	24.504669235290276	29.449487363677925	19.899170639326062
13	23.314569320185463	27.00816299875922	29.931692026382812	19.7455756546725
14	19.884542545549532	30.450728139489325	28.138183242996146	21.526546071964997
15	19.178475804871677	27.853980278194996	32.15986416770065	20.807679749232676
16	22.138836282896886	25.62006138575067	30.228955789198718	22.012146542153726
17	21.154313328544376	25.369555279827598	28.063736694312023	25.412394697316003
18	22.882779337817542	25.612224906941815	28.613596290733362	22.89139946450728
19	23.325801606478155	28.11937569385489	25.590805198197607	22.96401750146934
20	23.64552994187945	27.45066283549925	27.19728335401293	21.70652386860837
21	22.82426696271142	28.830666753738655	27.20877685626592	21.13628942728401
22	21.64487690197871	26.863710572715995	26.38855874093907	25.102853784366225
23	21.303990073793507	28.148370665447658	25.71697250702018	24.830666753738655
24	21.444001828511723	27.045255665121136	28.927055443087575	22.583687063279566
25	21.265852543590412	27.807745053222753	26.807287925292233	24.1191144778946
26	19.71736433096062	29.857767909619277	26.0675243257363	24.3573434336838
27	21.483967870436885	27.755240645203422	26.179585972702935	24.581205511656762
28	19.63351400770587	28.1948671063802	28.62352249722458	23.54809638868935
29	21.177039117090054	28.23169855678182	26.781427545223014	23.809834780905113
30	20.83249526546072	28.84503363155489	26.275191014170968	24.047280088813427
31	22.77620322601711	28.861490237053484	23.347743747142953	25.014562789786453
32	22.281721413178346	30.242539019134068	23.98067001893816	23.495069548749427
33	21.546137268987135	29.339254228433358	23.42845947887416	25.686149023705347
34	20.248677594201006	28.314765232155686	25.629203944361002	25.80735322928231
35	20.977992555345132	28.082544243453274	25.09397244171619	25.845490759485408
36	21.380526350159997	28.926794227127278	24.966499053092146	24.726180369620586
37	20.047802520734017	28.82596486645334	25.82145889113825	25.304773721674394
38	20.3356625089793	30.256383465029714	25.563899954287205	23.844054071703784
39	21.62763664859923	26.82870763403644	25.42623914321165	26.11741657415268
40	22.39848494743029	26.22921700515902	27.845882583425848	23.526415463984847
41	19.886109841311303	26.27937046953569	26.439495853196632	27.39502383595638
42	20.83641350486515	25.843401031803044	27.06719780578593	26.25298765754588
43	21.88467315352968	25.282047933128716	26.81852021158493	26.01475870175668
44	21.396721739698297	26.623653105204724	26.401358322993534	25.57826683210344
45	21.51217919414876	26.875204074968977	25.996473584536016	25.616143146346243
46	22.83419316920264	26.59988245281787	26.09416835368641	24.471756024293086
47	20.433096062169398	26.696793574087373	27.700385293541434	25.169725070201785
48	20.443805916541503	26.20518513681186	28.160909031541827	25.190099915104813
49	21.587931822634363	24.903807222621303	28.352641546398484	25.155619408345846
50	20.58669104682296	25.510873114347287	28.281852021158493	25.62058381767126
51	20.143668778162347	25.514007705870828	26.94364265656632	27.39868085940051
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2033.0
1	2127.5
2	2222.0
3	2116.0
4	2010.0
5	1489.5
6	969.0
7	1001.0
8	1033.0
9	1130.0
10	1227.0
11	1198.5
12	1170.0
13	1272.0
14	1374.0
15	1392.0
16	1410.0
17	1369.0
18	1328.0
19	1282.5
20	1237.0
21	1364.5
22	1492.0
23	1482.5
24	1473.0
25	1857.0
26	2685.0
27	3129.0
28	3744.0
29	4359.0
30	5456.5
31	6554.0
32	6616.5
33	6679.0
34	7626.0
35	8573.0
36	9184.5
37	9796.0
38	11229.0
39	12662.0
40	14419.5
41	16177.0
42	18018.5
43	19860.0
44	22525.5
45	25191.0
46	28496.5
47	31802.0
48	34305.0
49	36808.0
50	35358.5
51	33909.0
52	30474.5
53	27040.0
54	24522.0
55	22004.0
56	20674.0
57	19344.0
58	17525.5
59	15707.0
60	14633.0
61	13559.0
62	12878.0
63	12197.0
64	10706.0
65	9215.0
66	8213.0
67	7211.0
68	6334.0
69	5457.0
70	4793.5
71	4130.0
72	3641.0
73	3152.0
74	2981.5
75	2377.5
76	1944.0
77	1519.5
78	1095.0
79	857.5
80	620.0
81	514.5
82	409.0
83	261.5
84	114.0
85	87.0
86	60.0
87	40.0
88	20.0
89	13.0
90	6.0
91	7.5
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.47111349347079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55082270494944	19.655807710788725
2	10.619908747401166	5.834814369803212
3	3.812859740954324	3.1423050803532555
4	2.034328522390555	2.2354107888638266
5	1.2482625822765678	1.7145581533686256
6	0.9581825716336617	1.5793405303688415
7	0.7649254402802305	1.4709347508786925
8	0.6115379126367215	1.343970192288288
9	0.5507818352246732	1.3617531275039212
>10	7.143230036420147	45.89793495886311
>50	0.6374326832384587	10.727941153583918
>100	0.060755302621165595	3.132003937490758
>500	0.004979942837800459	1.0922477259074959
>1k	0.0019919771351201835	0.810977519937329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1808	0.47227845621367465	No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	1156	0.3019656501012212	TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	923	0.24110233135244563	TruSeq Adapter, Index 13 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.24057989943185532	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	838	0.21889897472735584	No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	749	0.19565075426108536	TruSeq Adapter, Index 13 (100% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	561	0.14654215372559262	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	441	0.11519623849017176	No Hit
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	434	0.11336772676810553	TruSeq Adapter, Index 13 (96% over 25bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	428	0.1118004310063345	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	424	0.11075556716515378	TruSeq Adapter, Index 13 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	416	0.10866583948279239	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.61215960295174E-4	0.0	0.0	0.21054006399791028	0.0
2	2.61215960295174E-4	0.0	0.0	1.0119506301835042	0.0
3	2.61215960295174E-4	0.0	0.0	1.2924965715405212	0.0
4	2.61215960295174E-4	0.0	0.0	1.6921569907921374	0.0
5	2.61215960295174E-4	0.0	0.0	2.967413308953177	0.0
6	2.61215960295174E-4	0.0	0.0	3.8680859400509373	0.0
7	2.61215960295174E-4	0.0	0.0	4.6603539476262	0.0
8	2.61215960295174E-4	0.0	0.0	5.896166655782668	0.0
9	2.61215960295174E-4	0.0	0.0	6.42434532749951	0.0
10	2.61215960295174E-4	0.0	0.0	7.628028472539672	0.0
11	2.61215960295174E-4	0.0	0.0	8.891791288447724	0.0
12	2.61215960295174E-4	0.0	0.0	9.940573369032847	0.0
13	2.61215960295174E-4	0.0	0.0	10.37027362371841	0.0
14	2.61215960295174E-4	0.0	0.0	10.530660223339646	0.0
15	2.61215960295174E-4	0.0	0.0	10.817475347743747	0.0
16	2.61215960295174E-4	0.0	0.0	11.419578136224123	0.0
17	2.61215960295174E-4	0.0	0.0	12.14392999412264	0.0
18	2.61215960295174E-4	0.0	0.0	13.063148958401358	0.0
19	5.22431920590348E-4	0.0	0.0	13.477437471429504	0.0
20	7.836478808855221E-4	0.0	0.0	13.871612355514921	0.0
21	7.836478808855221E-4	0.0	0.0	14.374191863122837	0.0
22	7.836478808855221E-4	0.0	0.0	14.877555018611638	0.0
23	7.836478808855221E-4	0.0	0.0	15.360804545157709	0.0
24	7.836478808855221E-4	0.0	0.0	15.76673414745641	0.0
25	7.836478808855221E-4	0.0	0.0	16.135832299353492	0.0
26	7.836478808855221E-4	0.0	0.0	16.511722066218248	0.0
27	0.001044863841180696	0.0	0.0	16.86279631685496	0.0
28	0.001044863841180696	0.0	0.0	17.196369098151898	0.0
29	0.001044863841180696	0.0	0.0	17.544831189185658	0.0
30	0.001044863841180696	0.0	0.0	17.956507542610854	0.0
31	0.001044863841180696	0.0	0.0	18.33448703715797	0.0
32	0.001044863841180696	0.0	0.0	18.71560112322863	0.0
33	0.001044863841180696	0.0	0.0	19.082609547443347	0.0
34	0.001044863841180696	0.0	0.0	19.41644354470058	0.0
35	0.001044863841180696	0.0	0.0	19.823940442761053	0.0
36	0.0013060798014758702	0.0	0.0	20.182067524325735	0.0
37	0.0013060798014758702	0.0	0.0	20.52243192059035	0.0
38	0.0013060798014758702	0.0	0.0	20.86775942010057	0.0
39	0.0013060798014758702	0.0	0.0	21.20812381636518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACTA	30	2.1616943E-6	45.000004	6
CACCGTA	30	2.1616943E-6	45.000004	25
GTAGTGC	30	2.1616943E-6	45.000004	29
TAATACG	20	7.027385E-4	45.0	1
CTGTCGA	20	7.027385E-4	45.0	12
ACAGTCG	20	7.027385E-4	45.0	1
CTCGTCA	25	3.8859143E-5	45.0	36
GTCGAGT	20	7.027385E-4	45.0	14
CGAGTAC	25	3.8859143E-5	45.0	17
GCGAATG	20	7.027385E-4	45.0	1
TATGGTC	50	2.1827873E-11	45.0	43
ATAATGC	20	7.027385E-4	45.0	33
GACCGAA	20	7.027385E-4	45.0	9
ACCGAAC	20	7.027385E-4	45.0	10
CGATTAC	20	7.027385E-4	45.0	13
TAGCACG	20	7.027385E-4	45.0	1
CGGATAT	35	1.2090823E-7	45.0	6
TACGAGC	20	7.027385E-4	45.0	4
TATTAGT	45	3.8380676E-10	45.0	30
TTGCGAG	20	7.027385E-4	45.0	1
>>END_MODULE