##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1172881
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973283734667028	33.0	31.0	34.0	30.0	34.0
2	32.07595911264655	33.0	31.0	34.0	30.0	34.0
3	32.05510192423613	33.0	31.0	34.0	30.0	34.0
4	35.71865943774347	37.0	35.0	37.0	33.0	37.0
5	35.62490823877273	37.0	35.0	37.0	33.0	37.0
6	35.58463134793726	37.0	35.0	37.0	33.0	37.0
7	35.8836326959001	37.0	35.0	37.0	35.0	37.0
8	35.80201827806913	37.0	35.0	37.0	35.0	37.0
9	37.50399145352342	39.0	37.0	39.0	35.0	39.0
10	36.986149490016466	39.0	37.0	39.0	33.0	39.0
11	36.91675114525685	39.0	37.0	39.0	33.0	39.0
12	36.8442467735431	39.0	35.0	39.0	33.0	39.0
13	36.83398742071873	39.0	37.0	39.0	33.0	39.0
14	37.86787321134881	40.0	37.0	41.0	33.0	41.0
15	37.99096242500305	40.0	37.0	41.0	33.0	41.0
16	38.03133224939273	40.0	37.0	41.0	33.0	41.0
17	37.96893035184303	40.0	37.0	41.0	33.0	41.0
18	37.950217455990845	40.0	37.0	41.0	33.0	41.0
19	37.90488549136698	40.0	37.0	41.0	33.0	41.0
20	37.85432537486753	40.0	36.0	41.0	33.0	41.0
21	37.68770318557467	40.0	36.0	41.0	33.0	41.0
22	37.71512455227768	40.0	36.0	41.0	33.0	41.0
23	37.664642875108385	40.0	36.0	41.0	33.0	41.0
24	37.60504347840915	40.0	36.0	41.0	33.0	41.0
25	37.469909564567935	39.0	35.0	41.0	32.0	41.0
26	37.4549881872074	39.0	35.0	41.0	33.0	41.0
27	37.42022506972148	39.0	35.0	41.0	32.0	41.0
28	37.28594631509932	39.0	35.0	41.0	32.0	41.0
29	37.23331522976329	39.0	35.0	41.0	32.0	41.0
30	37.0714471459594	39.0	35.0	41.0	31.0	41.0
31	37.0077851035186	39.0	35.0	41.0	31.0	41.0
32	36.86147614293351	39.0	35.0	41.0	31.0	41.0
33	36.74983992408437	39.0	35.0	41.0	31.0	41.0
34	36.58935049676821	39.0	35.0	41.0	30.0	41.0
35	36.49431442746536	39.0	35.0	41.0	30.0	41.0
36	36.34449189644985	39.0	35.0	41.0	30.0	41.0
37	36.263076987349955	39.0	35.0	41.0	30.0	41.0
38	36.11424858958411	39.0	35.0	41.0	29.0	41.0
39	36.02343630769021	39.0	35.0	40.0	29.0	41.0
40	35.912053311461264	38.0	35.0	40.0	29.0	41.0
41	35.77446987375531	38.0	35.0	40.0	28.0	41.0
42	35.667401040685284	38.0	35.0	40.0	28.0	41.0
43	35.60109422865576	38.0	34.0	40.0	28.0	41.0
44	35.478234364782104	38.0	34.0	40.0	27.0	41.0
45	35.40907389581722	38.0	34.0	40.0	27.0	41.0
46	35.23959037617627	38.0	34.0	40.0	27.0	41.0
47	35.10595107261521	37.0	34.0	40.0	26.0	41.0
48	35.02866957517429	37.0	34.0	40.0	26.0	41.0
49	34.94051911489742	37.0	34.0	40.0	26.0	41.0
50	34.816968643877765	37.0	34.0	40.0	26.0	41.0
51	33.34771046679075	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	10.0
11	11.0
12	18.0
13	34.0
14	53.0
15	80.0
16	191.0
17	345.0
18	568.0
19	957.0
20	1489.0
21	2445.0
22	3562.0
23	4962.0
24	6809.0
25	8921.0
26	11481.0
27	14047.0
28	16445.0
29	19266.0
30	23287.0
31	28641.0
32	36220.0
33	49705.0
34	79470.0
35	93605.0
36	101979.0
37	144050.0
38	224860.0
39	299289.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33211894471818	18.35036973060353	22.745103723225117	14.572407601453175
2	31.594424327787728	24.55313028346439	25.36668255347303	18.485762835274848
3	33.46869801795749	23.991862772097083	25.97816828817246	16.561270921772966
4	29.004647530312113	26.332935736873566	24.887776338776057	19.774640394038272
5	25.48996871805409	29.90738190830954	24.632081174475502	19.97056819916087
6	25.091292296490437	35.57385617125693	24.323695242739884	15.011156289512748
7	82.94396447721465	5.94263186120331	7.266892378681213	3.846511282900823
8	84.4674779453329	5.146813700622655	6.708608972265728	3.6770993817787136
9	79.23267577870219	7.002330159666667	8.318235183279462	5.446758878351683
10	41.60567014044903	26.324580243008455	15.8797866109179	16.189963005624612
11	31.41478120968794	26.917394006723615	22.071122304820353	19.596702478768094
12	26.826847736471134	24.108583905784133	28.3198380739393	20.74473028380543
13	25.765785275744086	24.582203991709303	29.15351173733738	20.498498995209232
14	22.849206355973024	26.471653987062627	28.860728411492726	21.81841124547162
15	22.361603606845026	25.572841575573314	30.571387890161066	21.4941669274206
16	25.434208585525724	24.768497400844584	28.619186430677964	21.17810758295172
17	24.699095645679314	24.337422125518273	27.312318982062116	23.651163246740293
18	26.643197391721753	23.553455124603435	27.151774135654	22.651573348020815
19	25.99351511363898	26.29542127462206	25.362675326823435	22.348388284915522
20	27.48087828176942	25.846867670292212	25.56883434892372	21.103419699014648
21	26.757360721164382	26.167616322542525	26.129931340008067	20.945091616285026
22	25.401042390489742	23.86201157662201	26.58249217098751	24.154453861900738
23	24.49873431319972	26.612162700222786	26.316224749143345	22.572878237434146
24	23.47365163217752	25.897938495039142	27.822089367975096	22.806320504808244
25	24.87822720293022	26.343081693709763	26.209734832433977	22.568956270926037
26	23.597960918456348	25.999824364108548	25.80662488351333	24.59558983392177
27	23.70777598068346	25.194201287257616	27.19542732809211	23.902595403966814
28	22.675019886928	27.33414557828117	26.74602112234745	23.244813412443378
29	23.78314594575238	26.556658348118862	26.060103284135387	23.600092421993367
30	24.368883117724646	24.953597167999142	27.30669181272439	23.37082790155182
31	25.412808290014077	26.489558616773568	24.879335584769468	23.218297508442888
32	25.13298450567449	26.14578972632347	25.113204152850972	23.608021615151067
33	24.466847020285947	25.94329689030686	24.606588392172778	24.983267697234417
34	23.207810511040762	26.597071655180706	26.752415632958503	23.44270220082003
35	23.424200750118725	26.587181478768944	26.372240662096154	23.616377109016174
36	24.591412086989216	28.08673684713112	24.898774897027064	22.423076168852596
37	23.495819268962496	27.923463676195624	25.113715713699857	23.467001341142023
38	23.12476713323858	28.045811979220396	26.009544020237346	22.819876867303673
39	23.60546381090665	25.899558437727272	25.689051148411473	24.805926602954607
40	24.47776031839547	25.93545295729064	27.245219250716822	22.341567473597067
41	22.053558715675333	26.191148121591194	26.579422805894204	25.17587035683927
42	23.244813412443378	25.798013609223787	26.665876589355612	24.291296388977226
43	22.967973733055615	24.539403400686002	27.4483089077238	25.04431395853458
44	23.38199698008579	25.575996200808092	26.632625134178145	24.40938168492797
45	24.195549250094427	25.0892460530949	25.682912418224866	25.032292278585803
46	23.14608216860875	26.620688714370853	25.641475989465256	24.59175312755514
47	22.15979285196026	27.216827623603756	27.647050297515264	22.97632922692072
48	22.249657041080894	27.19380738540397	26.72479134711876	23.83174422639637
49	23.462908854350953	26.725558688392088	26.951071762608482	22.860460694648477
50	21.890967625871678	26.621967616493063	27.295778514614867	24.19128624302039
51	22.39238251791955	25.970153834873273	26.09301369874693	25.544449948460247
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1740.0
1	2176.0
2	2612.0
3	4342.5
4	6073.0
5	4234.0
6	2395.0
7	2364.5
8	2334.0
9	2490.0
10	2646.0
11	2675.5
12	2705.0
13	2776.0
14	2847.0
15	2985.5
16	3124.0
17	2990.5
18	2857.0
19	3144.5
20	3432.0
21	3508.0
22	3584.0
23	3894.0
24	4204.0
25	5294.0
26	8446.0
27	10508.0
28	12659.0
29	14810.0
30	17167.5
31	19525.0
32	21403.5
33	23282.0
34	26042.0
35	28802.0
36	31928.0
37	35054.0
38	36528.0
39	38002.0
40	41068.5
41	44135.0
42	48468.0
43	52801.0
44	58889.0
45	64977.0
46	70987.5
47	76998.0
48	83432.5
49	89867.0
50	89143.0
51	88419.0
52	84957.0
53	81495.0
54	77562.0
55	73629.0
56	71076.0
57	68523.0
58	64380.0
59	60237.0
60	58693.5
61	57150.0
62	53783.5
63	50417.0
64	45437.5
65	40458.0
66	35239.0
67	30020.0
68	26482.5
69	22945.0
70	19915.5
71	16886.0
72	14972.0
73	13058.0
74	11473.0
75	8275.5
76	6663.0
77	5318.5
78	3974.0
79	2818.0
80	1662.0
81	1230.5
82	799.0
83	616.5
84	434.0
85	333.0
86	232.0
87	172.0
88	112.0
89	80.5
90	49.0
91	48.0
92	47.0
93	43.5
94	40.0
95	38.0
96	36.0
97	23.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1172881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.165997220290432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.83571753298718	17.843137446603176
2	8.431588844291712	4.075155051475707
3	3.161252945219084	2.2918448966039797
4	1.7138070085285477	1.6566342161686058
5	1.1553119929202775	1.3959633204739816
6	0.8851896869376464	1.2834894908378955
7	0.7094256362771096	1.2000784567992782
8	0.5964766822243752	1.1531563075681848
9	0.5307641260341336	1.1543799954933678
>10	7.682561548134171	44.94525704920681
>50	1.2250479598694681	18.88770804552112
>100	0.06752510707080489	2.750718964006348
>500	0.003553953003725631	0.5953535612117178
>1k	0.0017769765018628154	0.7671231980298706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3214	0.27402609471890155	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1789	0.15253039310893432	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1479	0.1260997492499239	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1368	0.11663587354556856	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7052028296135754E-4	0.0	0.0	0.045358395267721105	0.0
2	1.7052028296135754E-4	0.0	0.0	0.23199284496892694	0.0
3	1.7052028296135754E-4	0.0	0.0	0.3237327572021373	0.0
4	1.7052028296135754E-4	0.0	0.0	0.45656805762903485	0.0
5	1.7052028296135754E-4	0.0	0.0	0.8711881256495757	0.0
6	1.7052028296135754E-4	0.0	0.0	1.298426694609257	0.0
7	1.7052028296135754E-4	0.0	0.0	1.6392967402490108	0.0
8	1.7052028296135754E-4	0.0	0.0	2.172428404927695	0.0
9	1.7052028296135754E-4	0.0	0.0	2.427526748237886	0.0
10	1.7052028296135754E-4	0.0	0.0	2.9413043608004563	0.0
11	1.7052028296135754E-4	0.0	0.0	3.4986499056596534	0.0
12	1.7052028296135754E-4	0.0	0.0	3.948141371545792	0.0
13	1.7052028296135754E-4	0.0	0.0	4.130512814172964	0.0
14	1.7052028296135754E-4	0.0	0.0	4.211765729004051	0.0
15	1.7052028296135754E-4	0.0	0.0	4.340508542639876	0.0
16	2.557804244420363E-4	0.0	0.0	4.5996993727411395	0.0
17	2.557804244420363E-4	0.0	0.0	4.913456693390037	0.0
18	2.557804244420363E-4	0.0	0.0	5.308125888304099	0.0
19	2.557804244420363E-4	0.0	0.0	5.512494447433286	0.0
20	2.557804244420363E-4	0.0	0.0	5.723342777315005	0.0
21	2.557804244420363E-4	0.0	0.0	5.969233025345282	0.0
22	3.410405659227151E-4	0.0	0.0	6.240019234687918	0.0
23	3.410405659227151E-4	0.0	0.0	6.524276546384501	0.0
24	3.410405659227151E-4	0.0	0.0	6.7607029187104235	0.0
25	4.2630070740339385E-4	0.0	0.0	6.974109052836562	0.0
26	4.2630070740339385E-4	0.0	0.0	7.167052753007338	0.0
27	4.2630070740339385E-4	0.0	0.0	7.390775364252639	0.0
28	4.2630070740339385E-4	0.0	0.0	7.598895369606977	0.0
29	4.2630070740339385E-4	0.0	0.0	7.833531278961805	0.0
30	5.115608488840726E-4	0.0	0.0	8.104488008587401	0.0
31	5.115608488840726E-4	0.0	0.0	8.31908778469427	0.0
32	5.968209903647514E-4	0.0	0.0	8.554491035322425	0.0
33	5.968209903647514E-4	0.0	0.0	8.782988214490643	0.0
34	5.968209903647514E-4	0.0	0.0	9.018220944835836	0.0
35	5.968209903647514E-4	0.0	0.0	9.278946457483752	0.0
36	5.968209903647514E-4	0.0	0.0	9.511365603160082	0.0
37	5.968209903647514E-4	0.0	0.0	9.754101225955575	0.0
38	5.968209903647514E-4	0.0	0.0	10.015594079876816	0.0
39	5.968209903647514E-4	0.0	0.0	10.380081184706718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	20	7.0333446E-4	45.000004	20
ACGCTAG	45	3.8562575E-10	45.000004	1
ACGTCAC	20	7.0333446E-4	45.000004	17
ATCGATT	25	3.8908554E-5	45.0	32
CGTGTAC	230	0.0	40.108692	18
ACACGAC	855	0.0	39.73684	26
AACGGGC	250	0.0	39.6	4
TGGTCAA	900	0.0	39.25	14
CACGACC	880	0.0	38.863636	27
GCGAGAC	905	0.0	38.78453	21
TCAAGCG	930	0.0	38.70968	17
GTCAAGC	925	0.0	38.675674	16
CAACGTT	35	6.2483996E-6	38.571426	31
CTGCGTA	35	6.2483996E-6	38.571426	21
TTACGCG	70	0.0	38.571426	1
AACACGT	825	0.0	38.18182	41
ACGACCA	890	0.0	38.17416	28
AGACACG	900	0.0	38.0	24
CATGGTC	930	0.0	37.741936	12
ATGGTCA	935	0.0	37.540104	13
>>END_MODULE