FastQCFastQC Report
Sat 18 Jun 2016
SRR3553927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720693
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18420.25558733052770044No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.2263099544466229No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC11720.16262125482001352No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10310.14305675232033613No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9040.12543482453693874No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC8870.1230759838100273No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC8840.12265971779939586No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8770.12168843044125584No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG7480.10378899198410418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG207.0315326E-445.01
CGTGCCG253.889353E-545.012
CACGATA253.889353E-545.019
CCGCTTA207.0315326E-445.018
TACGCAT453.8380676E-1045.016
TCGACAG207.0315326E-445.01
ATTCGCG302.16437E-644.9999961
CGGTCTA650.044.99999631
CGTTTAG302.16437E-644.9999962
ATAACGG1000.042.752
CGACGGT750.042.028
ATACCGG603.6379788E-1241.2499962
TTACGAG556.002665E-1140.9090921
GCGTAAG556.002665E-1140.9090921
GGCCGAT1550.040.6451648
ACGCATT501.0804797E-940.517
ACTAAGG501.0804797E-940.52
AACACGT1400.040.17857441
TAGCACG451.9266736E-840.01
TACGAGG1300.039.807692