##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720693
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.909943623706628	33.0	31.0	34.0	30.0	34.0
2	31.99810460209826	33.0	31.0	34.0	30.0	34.0
3	31.991465159228685	33.0	31.0	34.0	30.0	34.0
4	35.671278894064464	37.0	35.0	37.0	33.0	37.0
5	35.57817545057327	37.0	35.0	37.0	33.0	37.0
6	35.547331526738844	37.0	35.0	37.0	33.0	37.0
7	35.86170255573455	37.0	35.0	37.0	35.0	37.0
8	35.773064258984064	37.0	35.0	37.0	35.0	37.0
9	37.49669970431238	39.0	37.0	39.0	35.0	39.0
10	36.94336145904012	39.0	37.0	39.0	33.0	39.0
11	36.88288772056895	39.0	37.0	39.0	33.0	39.0
12	36.82994700933685	39.0	35.0	39.0	33.0	39.0
13	36.80335316147097	39.0	36.0	39.0	33.0	39.0
14	37.88022500565428	40.0	37.0	41.0	33.0	41.0
15	37.99717632889455	40.0	37.0	41.0	33.0	41.0
16	38.02817288359954	40.0	37.0	41.0	33.0	41.0
17	37.9730384504914	40.0	37.0	41.0	33.0	41.0
18	37.94182821256763	40.0	37.0	41.0	33.0	41.0
19	37.84555420962879	40.0	36.0	41.0	33.0	41.0
20	37.791120490971885	40.0	36.0	41.0	33.0	41.0
21	37.66165621145203	40.0	36.0	41.0	33.0	41.0
22	37.659018472498	39.0	36.0	41.0	33.0	41.0
23	37.585111829863756	39.0	36.0	41.0	33.0	41.0
24	37.56858745679506	39.0	35.0	41.0	33.0	41.0
25	37.4320855065888	39.0	35.0	41.0	32.0	41.0
26	37.3882263321553	39.0	35.0	41.0	33.0	41.0
27	37.364765579796114	39.0	35.0	41.0	32.0	41.0
28	37.22384427211031	39.0	35.0	41.0	32.0	41.0
29	37.20279231239932	39.0	35.0	41.0	32.0	41.0
30	37.038777953997055	39.0	35.0	41.0	31.0	41.0
31	36.9208484056318	39.0	35.0	41.0	31.0	41.0
32	36.793536221386915	39.0	35.0	41.0	31.0	41.0
33	36.72983642133335	39.0	35.0	41.0	31.0	41.0
34	36.59169577059858	39.0	35.0	41.0	30.0	41.0
35	36.49083174111584	39.0	35.0	41.0	30.0	41.0
36	36.31994344332469	39.0	35.0	41.0	30.0	41.0
37	36.25039371826839	39.0	35.0	41.0	30.0	41.0
38	36.132226898277075	39.0	35.0	40.0	30.0	41.0
39	36.104534108143135	39.0	35.0	40.0	30.0	41.0
40	35.976773744160134	38.0	35.0	40.0	29.0	41.0
41	35.835741432204834	38.0	35.0	40.0	29.0	41.0
42	35.702605686471216	38.0	35.0	40.0	28.0	41.0
43	35.63256754262911	38.0	35.0	40.0	28.0	41.0
44	35.53308551630167	38.0	34.0	40.0	28.0	41.0
45	35.46861007391497	38.0	34.0	40.0	27.0	41.0
46	35.28079084991806	38.0	34.0	40.0	27.0	41.0
47	35.15932720312255	37.0	34.0	40.0	26.0	41.0
48	35.071453448278255	37.0	34.0	40.0	26.0	41.0
49	34.999099477863666	37.0	34.0	40.0	26.0	41.0
50	34.877860614713896	37.0	34.0	40.0	26.0	41.0
51	33.42116129891646	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	7.0
11	4.0
12	15.0
13	17.0
14	21.0
15	49.0
16	101.0
17	224.0
18	399.0
19	668.0
20	995.0
21	1480.0
22	2184.0
23	3057.0
24	4221.0
25	5457.0
26	6865.0
27	8309.0
28	9812.0
29	11382.0
30	13823.0
31	17366.0
32	22331.0
33	30532.0
34	50268.0
35	61943.0
36	61893.0
37	88175.0
38	139181.0
39	179848.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05176684108213	19.337915034557014	22.42605381209475	14.184264312266109
2	30.15153470340353	26.185490909444102	25.322987735415776	18.33998665173659
3	31.67728838770461	25.246672300133344	26.32313620362623	16.752903108535815
4	28.444705304477775	26.011491717000162	25.990539661131717	19.553263317390346
5	24.336159779545522	31.36161999630911	24.692622239982907	19.609597984162466
6	24.38930307356947	36.78223598675164	24.271916058571403	14.556544881107488
7	81.2216852390685	6.753361001147507	8.03171391979664	3.9932398399873454
8	82.68499902177487	6.224425656971831	7.02532146142671	4.0652538598265835
9	77.6204292257591	7.763916119623751	8.937925024941272	5.6777296296758815
10	40.03549361517317	26.76201933416864	16.68338668476036	16.519100365897824
11	30.797579551903514	27.229763574781497	22.430077716864186	19.542579156450802
12	27.2024287734167	23.94153960146692	29.174974642462185	19.681056982654198
13	24.44882911308976	25.468125817789268	29.282371273205094	20.800673795915873
14	21.627239337693027	29.25351016313465	28.101424601043718	21.017825898128606
15	21.102882919634297	26.635335711599804	31.497738981785588	20.764042386980307
16	23.388877094685252	25.16605544940772	29.934105090517043	21.51096236538998
17	22.336417864472114	25.392920425201854	27.393217361622774	24.87744434870326
18	24.422881865093736	26.079343076733092	27.850832462643595	21.64694259552958
19	24.592995908105113	27.18688817568646	25.912281651133007	22.30783426507542
20	25.738282458689067	26.633115626209776	25.972362712000812	21.65623920310035
21	24.8249948313637	27.560140031885975	26.46314033853527	21.151724798215053
22	23.1943421123835	25.487551564952067	25.84290398269443	25.47520233997
23	22.674564620441714	27.65768503371061	26.748837577165315	22.918912768682365
24	23.056974328875125	26.8320907792916	27.17953414283197	22.931400749001305
25	23.293690933587534	27.30718905275894	26.40486309704687	22.994256916606655
26	22.660966597427752	27.817808692466834	26.035635145616787	23.485589564488624
27	23.520000888034158	26.584273747629016	27.077687725564143	22.81803763877268
28	21.80984136102335	27.24474915116423	28.426112089336236	22.51929739847619
29	22.725904095086257	26.882736477251758	26.821545373688938	23.56981405397305
30	24.01119478057925	25.966396232515095	27.343681706357632	22.678727280548028
31	25.13192163653595	26.88939673342186	24.78517204967996	23.193509580362235
32	23.66111506563821	28.310390138380697	24.60104371764399	23.4274510783371
33	23.025338112067136	26.638804595021735	24.881745764146455	25.454111528764674
34	22.481000925498094	26.82265541638395	26.217682147599604	24.47866151051835
35	23.947783591626393	26.42914528100037	24.78336823030056	24.83970289707268
36	24.71718193461016	28.3152465751714	25.08280224728144	21.884769242937008
37	23.074596256658523	27.91424365159645	26.84555004696868	22.165610044776347
38	21.8065112329383	28.5854032160712	25.780325325762842	23.827760225227664
39	22.386924807095394	25.948635549394815	26.410413310521957	25.254026332987834
40	23.224590775822715	26.482011064350562	27.373097837775585	22.920300322051137
41	21.943601505772918	27.2558495781144	26.934630973243806	23.86591794286888
42	22.90129084089897	27.05784571239071	26.783665166721477	23.257198279988845
43	23.363901134047367	24.96999415840032	27.650747266866755	24.015357440685563
44	23.289667028818094	24.935721590191662	27.469949062915834	24.304662318074406
45	23.858425154677512	25.200744283627007	25.776717687004037	25.16411287469144
46	24.299528370609956	26.312729553360448	25.73855996936282	23.64918210666678
47	22.894769340065743	26.880377636524845	27.638814307895316	22.586038715514096
48	22.344188163337233	27.12056312465918	27.019687994749496	23.51556071725409
49	22.129672412525167	26.118888347743074	28.037319635406476	23.714119604325283
50	21.6723348221781	25.597584547095643	28.618565741584835	24.111514889141425
51	21.509713567358084	25.95501829489117	26.4344179838017	26.100850153949047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2235.0
1	2575.5
2	2916.0
3	3211.5
4	3507.0
5	2614.5
6	1722.0
7	1699.0
8	1676.0
9	1779.0
10	1882.0
11	1855.5
12	1829.0
13	1854.0
14	1879.0
15	1987.5
16	2096.0
17	2087.5
18	2079.0
19	2145.0
20	2211.0
21	2276.5
22	2342.0
23	2909.0
24	3476.0
25	3893.0
26	5203.5
27	6097.0
28	7297.5
29	8498.0
30	9909.5
31	11321.0
32	12920.0
33	14519.0
34	16316.0
35	18113.0
36	19773.5
37	21434.0
38	22243.5
39	23053.0
40	25975.0
41	28897.0
42	32908.5
43	36920.0
44	41291.5
45	45663.0
46	50228.0
47	54793.0
48	58307.5
49	61822.0
50	60903.0
51	59984.0
52	55175.5
53	50367.0
54	45773.5
55	41180.0
56	38573.5
57	35967.0
58	34716.5
59	33466.0
60	31947.0
61	30428.0
62	28252.0
63	26076.0
64	22868.5
65	19661.0
66	17916.5
67	16172.0
68	13990.0
69	11808.0
70	10617.0
71	9426.0
72	8379.0
73	7332.0
74	6322.0
75	4536.5
76	3761.0
77	2845.5
78	1930.0
79	1582.0
80	1234.0
81	950.5
82	667.0
83	464.5
84	262.0
85	212.0
86	162.0
87	115.0
88	68.0
89	60.5
90	53.0
91	54.5
92	56.0
93	41.0
94	26.0
95	15.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.991857372296426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35944652639978	18.583806818706865
2	9.612596288449984	4.80473270836816
3	3.4579360274302813	2.592607320001887
4	1.7410861503237673	1.7405190696708903
5	1.0981155791986028	1.3721973966814083
6	0.7964456129065015	1.19427930975303
7	0.5992338327581879	1.0483176536663013
8	0.5125483279533107	1.0247627766894527
9	0.4181369659125642	0.9405017472754419
>10	5.794795181117682	35.28535736369898
>50	1.4604306573702839	24.20631070981088
>100	0.13972738780810173	5.177867531287377
>500	0.00726582416602129	1.2359052555189165
>1k	0.002235638204929628	0.792834338870408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1842	0.25558733052770044	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1631	0.2263099544466229	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	1172	0.16262125482001352	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1031	0.14305675232033613	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	904	0.12543482453693874	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC	887	0.1230759838100273	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	884	0.12265971779939586	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	877	0.12168843044125584	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	748	0.10378899198410418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7751067375428925E-4	0.0	0.0	0.0879708835801097	0.0
2	2.7751067375428925E-4	0.0	0.0	0.49993547876835215	0.0
3	2.7751067375428925E-4	0.0	0.0	0.67060454312724	0.0
4	2.7751067375428925E-4	0.0	0.0	0.921890458211749	0.0
5	2.7751067375428925E-4	0.0	0.0	1.6928151099011646	0.0
6	2.7751067375428925E-4	0.0	0.0	2.2951520272848493	0.0
7	2.7751067375428925E-4	0.0	0.0	2.8142357425422477	0.0
8	2.7751067375428925E-4	0.0	0.0	3.5757250313240174	0.0
9	2.7751067375428925E-4	0.0	0.0	3.919838266779336	0.0
10	2.7751067375428925E-4	0.0	0.0	4.785810324229596	0.0
11	2.7751067375428925E-4	0.0	0.0	5.831886808946389	0.0
12	2.7751067375428925E-4	0.0	0.0	6.673299171769394	0.0
13	2.7751067375428925E-4	0.0	0.0	6.959828942420698	0.0
14	2.7751067375428925E-4	0.0	0.0	7.075273382702482	0.0
15	4.162660106314339E-4	0.0	0.0	7.292147974241459	0.0
16	4.162660106314339E-4	0.0	0.0	7.751983160652316	0.0
17	4.162660106314339E-4	0.0	0.0	8.31352600899412	0.0
18	4.162660106314339E-4	0.0	0.0	9.007857714727352	0.0
19	4.162660106314339E-4	0.0	0.0	9.335459065094291	0.0
20	6.937766843857232E-4	0.0	0.0	9.627955315231311	0.0
21	6.937766843857232E-4	0.0	0.0	10.003566012157743	0.0
22	6.937766843857232E-4	0.0	0.0	10.40165507365827	0.0
23	8.325320212628679E-4	0.0	0.0	10.814868466878407	0.0
24	8.325320212628679E-4	0.0	0.0	11.152876467511131	0.0
25	8.325320212628679E-4	0.0	0.0	11.43968374883619	0.0
26	8.325320212628679E-4	0.0	0.0	11.703318888902764	0.0
27	8.325320212628679E-4	0.0	0.0	11.995537628366032	0.0
28	8.325320212628679E-4	0.0	0.0	12.303990742243924	0.0
29	8.325320212628679E-4	0.0	0.0	12.601898450519153	0.0
30	8.325320212628679E-4	0.0	0.0	12.95392074017647	0.0
31	8.325320212628679E-4	0.0	0.0	13.257239906589907	0.0
32	8.325320212628679E-4	0.0	0.0	13.563611690414643	0.0
33	8.325320212628679E-4	0.0	0.0	13.866375835480572	0.0
34	8.325320212628679E-4	0.0	0.0	14.174828949358465	0.0
35	8.325320212628679E-4	0.0	0.0	14.520746004193187	0.0
36	8.325320212628679E-4	0.0	0.0	14.830309160766095	0.0
37	8.325320212628679E-4	0.0	0.0	15.133905837853288	0.0
38	8.325320212628679E-4	0.0	0.0	15.450129250596301	0.0
39	8.325320212628679E-4	0.0	0.0	15.762190003233	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	20	7.0315326E-4	45.0	1
CGTGCCG	25	3.889353E-5	45.0	12
CACGATA	25	3.889353E-5	45.0	19
CCGCTTA	20	7.0315326E-4	45.0	18
TACGCAT	45	3.8380676E-10	45.0	16
TCGACAG	20	7.0315326E-4	45.0	1
ATTCGCG	30	2.16437E-6	44.999996	1
CGGTCTA	65	0.0	44.999996	31
CGTTTAG	30	2.16437E-6	44.999996	2
ATAACGG	100	0.0	42.75	2
CGACGGT	75	0.0	42.0	28
ATACCGG	60	3.6379788E-12	41.249996	2
TTACGAG	55	6.002665E-11	40.909092	1
GCGTAAG	55	6.002665E-11	40.909092	1
GGCCGAT	155	0.0	40.645164	8
ACGCATT	50	1.0804797E-9	40.5	17
ACTAAGG	50	1.0804797E-9	40.5	2
AACACGT	140	0.0	40.178574	41
TAGCACG	45	1.9266736E-8	40.0	1
TACGAGG	130	0.0	39.80769	2
>>END_MODULE