FastQCFastQC Report
Sat 18 Jun 2016
SRR3553926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902059
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG56830.6300031372670745No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC39880.44209968527557514No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC31820.35274854527253763No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.2949917910025841No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12880.14278445201477952No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC12510.13868272474416862No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC11530.12781869035173973Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10960.12149981320512294No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9720.10775348397388641No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT9150.10143460682726961No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG253.8901362E-545.01
CGATAGA207.0324785E-445.040
ATCGATA207.0324785E-445.028
TAATCGA207.0324785E-445.026
CGGACGT207.0324785E-445.044
CCGATGA19200.043.4765618
GTCGAAT4350.043.44827343
CGATGAA20300.042.8940919
GATGAAT21050.042.75534420
ATGAATG21150.042.12766321
AGTACGG750.042.02
GACGTAG650.041.538461
TCGAATG4550.041.5384644
GGTCGAA4650.041.12903242
CGTAAGG1150.041.086962
TATTACG501.0804797E-940.51
CGGTCGA2750.040.09090841
CGAATGC3950.039.87341745
CCACCGA23300.039.39914315
AACCCGT403.4571167E-739.37538