##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902059
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.904903116093294	33.0	31.0	34.0	30.0	34.0
2	32.00562380066049	33.0	31.0	34.0	30.0	34.0
3	31.983630782465447	33.0	31.0	34.0	30.0	34.0
4	35.67859419394962	37.0	35.0	37.0	33.0	37.0
5	35.56627227265622	37.0	35.0	37.0	33.0	37.0
6	35.522746294865414	37.0	35.0	37.0	33.0	37.0
7	35.77728507780533	37.0	35.0	37.0	35.0	37.0
8	35.631653805349764	37.0	35.0	37.0	35.0	37.0
9	37.3209923075985	39.0	37.0	39.0	34.0	39.0
10	36.85027697744826	39.0	37.0	39.0	32.0	39.0
11	36.778239560826954	39.0	37.0	39.0	32.0	39.0
12	36.70226670317574	39.0	35.0	39.0	32.0	39.0
13	36.663088556291775	39.0	35.0	39.0	32.0	39.0
14	37.73909134546631	40.0	36.0	41.0	33.0	41.0
15	37.87416122448754	40.0	36.0	41.0	33.0	41.0
16	37.935616184750664	40.0	37.0	41.0	33.0	41.0
17	37.75216698686006	40.0	36.0	41.0	33.0	41.0
18	37.769584916285964	40.0	36.0	41.0	33.0	41.0
19	37.67408007680207	40.0	36.0	41.0	33.0	41.0
20	37.41760683059534	39.0	35.0	41.0	32.0	41.0
21	37.42059776577807	39.0	35.0	41.0	32.0	41.0
22	37.38510008768828	39.0	35.0	41.0	32.0	41.0
23	37.1783674903748	39.0	35.0	41.0	31.0	41.0
24	37.24281338582066	39.0	35.0	41.0	32.0	41.0
25	37.207102861342776	39.0	35.0	41.0	32.0	41.0
26	37.04865757117882	39.0	35.0	41.0	31.0	41.0
27	36.91638573530113	39.0	35.0	41.0	31.0	41.0
28	36.81690332893968	39.0	35.0	41.0	31.0	41.0
29	36.73529891060341	39.0	35.0	41.0	30.0	41.0
30	36.59521938143735	39.0	35.0	41.0	30.0	41.0
31	36.57834687088095	39.0	35.0	41.0	30.0	41.0
32	36.268108848756015	39.0	35.0	41.0	30.0	41.0
33	36.11276756841848	39.0	35.0	41.0	29.0	41.0
34	35.86828688589105	39.0	35.0	41.0	28.0	41.0
35	35.74287602030466	39.0	35.0	41.0	27.0	41.0
36	35.571975890712245	38.0	35.0	40.0	26.0	41.0
37	35.43989140399907	38.0	35.0	40.0	26.0	41.0
38	35.426936597273574	38.0	35.0	40.0	26.0	41.0
39	35.30320854844306	38.0	34.0	40.0	26.0	41.0
40	35.15340570849578	38.0	34.0	40.0	25.0	41.0
41	35.072183748513126	38.0	34.0	40.0	24.0	41.0
42	34.86009008279946	38.0	34.0	40.0	24.0	41.0
43	34.782243733503016	38.0	34.0	40.0	23.0	41.0
44	34.75344517376358	38.0	34.0	40.0	23.0	41.0
45	34.74107791175522	37.0	34.0	40.0	23.0	41.0
46	34.60366450531506	37.0	34.0	40.0	23.0	41.0
47	34.43655681058556	37.0	33.0	40.0	23.0	41.0
48	34.4316048063375	37.0	33.0	40.0	23.0	41.0
49	34.29693068856915	37.0	33.0	40.0	23.0	41.0
50	34.12396639244218	36.0	33.0	40.0	23.0	41.0
51	32.68462927591211	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	2.0
11	14.0
12	14.0
13	20.0
14	29.0
15	59.0
16	130.0
17	245.0
18	513.0
19	1126.0
20	2456.0
21	4425.0
22	6443.0
23	7949.0
24	8838.0
25	10086.0
26	11119.0
27	12186.0
28	13545.0
29	15416.0
30	18365.0
31	23235.0
32	29110.0
33	39242.0
34	62288.0
35	73779.0
36	75342.0
37	104467.0
38	161510.0
39	220046.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61974327621586	18.165108934116283	21.39993060320888	14.815217186458979
2	30.121422212959466	28.530617176925233	23.98823136845816	17.359729241657142
3	30.232501421747358	27.637770921857662	26.224116160916306	15.905611495478677
4	27.157203686233384	24.561364611405683	29.445967503234264	18.835464199126665
5	27.902942047027967	28.97626430200242	23.37895858253174	19.741835068437872
6	23.511322430129294	39.44575687399605	23.507996705315286	13.534923990559374
7	78.26627748295843	6.018009908442796	11.934363495070722	3.7813491135280506
8	79.86639454847189	9.169245027209971	7.534318708643226	3.430041715674917
9	74.77881158549496	7.195538207589526	8.752420850520865	9.273229356394648
10	43.46201301688693	24.870435304120907	15.991747768161508	15.675803910830666
11	33.702119262708976	25.882453365023792	21.624527885648277	18.790899486618944
12	26.771973895277362	23.15181157773494	27.77201934684982	22.304195180137885
13	27.0871417501516	23.482166909259817	28.939681329048323	20.491010011540265
14	22.075607027921677	29.29974646891168	27.383796403561185	21.240850099605456
15	21.70024355391388	24.859460412234675	30.127297660130882	23.312998373720568
16	23.970272454462513	23.438599914196303	28.500242223623957	24.090885407717234
17	23.42285815007666	26.44450085859129	27.26983489993448	22.86280609139757
18	24.19930403665392	24.832965471216404	27.011093509404592	23.956636982725076
19	25.846757252020105	25.80529654933879	25.089267996882686	23.25867820175842
20	28.218996761852605	25.49744528905537	25.85307612916672	20.4304818199253
21	25.61639537990309	28.199929272918954	25.964266195448417	20.219409151729543
22	23.75487634400854	23.890455058926303	28.304689604560235	24.049978992504926
23	25.625818266876117	26.924624664240365	26.043751018503226	21.405806050380296
24	22.949718366537	27.083150880374784	27.00898721702239	22.958143536065823
25	23.219656363940718	28.99455578847947	25.526157379949648	22.259630467630164
26	22.41117266165517	25.48225781240473	28.252143152498892	23.854426373441203
27	25.197797483313177	25.060888478469813	26.84990671341897	22.891407324798045
28	22.159082720753297	28.84645017676227	27.587441619672326	21.4070254828121
29	24.278012857252133	25.52017107528443	28.173212616913084	22.028603450550353
30	22.65683286791662	28.736036112937178	26.52797655142291	22.079154467723285
31	25.105785763458933	26.231100183025724	23.78502958232222	24.878084471193127
32	27.240346806583602	26.741488084482278	24.621781945526845	21.396383163407272
33	26.221677296052697	26.672423865844696	23.52617733429853	23.579721503804073
34	23.868283560166244	26.868419914883617	25.134497854353206	24.128798670596936
35	26.062375077461674	25.74122091792222	25.573826102283775	22.62257790233233
36	25.326281318627718	29.263717783426586	23.444918791342918	21.965082106602782
37	23.3254144130262	29.293871021740266	25.242251338327094	22.138463226906442
38	23.70920305656282	28.670519334101208	23.97559361416493	23.644683995171047
39	24.373682874401787	28.319987938704678	24.72155368994711	22.58477549694643
40	23.955860980268476	26.472215232041364	26.69282164470395	22.879102142986213
41	22.164847310430915	27.09556691968042	25.731908888442995	25.007676881445672
42	23.46132570042536	26.630298018200584	25.873806480507373	24.034569800866684
43	24.697386756298645	25.221299271998838	25.787781065318345	24.293532906384172
44	23.302134339328138	25.598325608413642	26.51921880941269	24.580321242845535
45	23.822721130214322	25.559636343077337	25.90650944117846	24.711133085529884
46	24.95834529670454	27.977659997849365	24.667011802997365	22.39698290244873
47	22.99339621909432	27.725015769478496	27.342224843386077	21.939363168041115
48	22.781436690948155	27.53378659267299	26.40326187089758	23.281514845481283
49	22.764032064421507	26.33319993481579	28.264780906792126	22.637987093970573
50	22.87899128549241	26.958547057343257	27.00289005486337	23.159571602300957
51	23.183405963468022	25.72969173856699	26.194295495084024	24.892606802880966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1617.0
1	2231.0
2	2845.0
3	4164.5
4	5484.0
5	3952.0
6	2420.0
7	2324.0
8	2228.0
9	2349.5
10	2471.0
11	2486.0
12	2501.0
13	2419.5
14	2338.0
15	2340.0
16	2342.0
17	2403.5
18	2465.0
19	2538.0
20	2611.0
21	3060.0
22	3509.0
23	3740.0
24	3971.0
25	5168.0
26	6678.0
27	6991.0
28	9427.5
29	11864.0
30	13541.5
31	15219.0
32	17084.0
33	18949.0
34	20842.5
35	22736.0
36	25719.5
37	28703.0
38	29462.5
39	30222.0
40	31135.0
41	32048.0
42	37315.0
43	42582.0
44	46287.5
45	49993.0
46	60073.5
47	70154.0
48	68040.0
49	65926.0
50	64462.5
51	62999.0
52	60620.0
53	58241.0
54	56915.0
55	55589.0
56	51909.5
57	48230.0
58	47416.5
59	46603.0
60	45657.0
61	44711.0
62	41807.0
63	38903.0
64	34141.0
65	29379.0
66	25062.0
67	20745.0
68	18786.0
69	16827.0
70	15411.0
71	13995.0
72	12169.5
73	10344.0
74	8508.0
75	5539.5
76	4407.0
77	3660.0
78	2913.0
79	2147.5
80	1382.0
81	1030.5
82	679.0
83	574.0
84	469.0
85	332.5
86	196.0
87	138.5
88	81.0
89	76.0
90	71.0
91	49.0
92	27.0
93	19.0
94	11.0
95	18.0
96	25.0
97	15.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	902059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.5657042060525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99649832911518	17.673452961136395
2	7.858523865839949	3.7038329783717683
3	2.9291703158417524	2.0708388369682833
4	1.697519196932415	1.600129411160203
5	1.1379268545980001	1.3408023831790092
6	0.8531304610573559	1.2062772056670505
7	0.6741684829692715	1.1121078538287894
8	0.5874797954546342	1.1075500069372908
9	0.5461201329665207	1.158273496301318
>10	7.247479882530994	40.93445571047181
>50	1.364843202409628	20.882696867761787
>100	0.095761836360294	3.622330804120715
>500	0.007585095949330218	1.3191611810713486
>1k	0.0033184794778319702	1.6331992976017993
>5k	4.740684968331386E-4	0.6348910054224656
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	5683	0.6300031372670745	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	3988	0.44209968527557514	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	3182	0.35274854527253763	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2661	0.2949917910025841	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1288	0.14278445201477952	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1251	0.13868272474416862	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	1153	0.12781869035173973	Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1096	0.12149981320512294	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	972	0.10775348397388641	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	915	0.10143460682726961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.217149876005893E-4	0.0	0.0	0.037358975410699304	0.0
2	2.217149876005893E-4	0.0	0.0	0.27625687455033426	0.0
3	2.217149876005893E-4	0.0	0.0	0.35186168532213524	0.0
4	2.217149876005893E-4	0.0	0.0	0.5386565623756318	0.0
5	2.217149876005893E-4	0.0	0.0	1.2841732081826134	0.0
6	2.217149876005893E-4	0.0	0.0	1.6335960286411422	0.0
7	2.217149876005893E-4	0.0	0.0	1.9467684486269745	0.0
8	2.217149876005893E-4	0.0	0.0	2.335989109359809	0.0
9	2.217149876005893E-4	0.0	0.0	2.5070422222936637	0.0
10	2.217149876005893E-4	0.0	0.0	3.494006489597687	0.0
11	2.217149876005893E-4	0.0	0.0	3.989539486885004	0.0
12	2.217149876005893E-4	0.0	0.0	4.728626398051569	0.0
13	2.217149876005893E-4	0.0	0.0	4.911762977809656	0.0
14	2.217149876005893E-4	0.0	0.0	5.039027380692394	0.0
15	2.217149876005893E-4	0.0	0.0	5.211965071020853	0.0
16	2.217149876005893E-4	0.0	0.0	5.468821884156136	0.0
17	2.217149876005893E-4	0.0	0.0	5.738094736597052	0.0
18	2.217149876005893E-4	0.0	0.0	6.072551795392541	0.0
19	2.217149876005893E-4	0.0	0.0	6.407895714138432	0.0
20	3.32572481400884E-4	0.0	0.0	6.607771775460363	0.0
21	3.32572481400884E-4	0.0	0.0	6.851990834302413	0.0
22	3.32572481400884E-4	0.0	0.0	7.0953230331940595	0.0
23	4.434299752011786E-4	0.0	0.0	7.3239111854102665	0.0
24	4.434299752011786E-4	0.0	0.0	7.529884408891214	0.0
25	4.434299752011786E-4	0.0	0.0	7.6990529444304645	0.0
26	5.542874690014733E-4	0.0	0.0	7.863676322723902	0.0
27	6.65144962801768E-4	0.0	0.0	8.038055160471766	0.0
28	6.65144962801768E-4	0.0	0.0	8.243695811471312	0.0
29	6.65144962801768E-4	0.0	0.0	8.421178659045584	0.0
30	6.65144962801768E-4	0.0	0.0	8.63058846483434	0.0
31	7.760024566020626E-4	0.0	0.0	8.80740616744581	0.0
32	7.760024566020626E-4	0.0	0.0	8.985443302489083	0.0
33	7.760024566020626E-4	0.0	0.0	9.180774206565202	0.0
34	7.760024566020626E-4	0.0	0.0	9.353268466918461	0.0
35	7.760024566020626E-4	0.0	0.0	9.557578827992405	0.0
36	7.760024566020626E-4	0.0	0.0	9.74093712273809	0.0
37	8.868599504023572E-4	0.0	0.0	9.930503437136595	0.0
38	8.868599504023572E-4	0.0	0.0	10.126499486175517	0.0
39	8.868599504023572E-4	0.0	0.0	10.326708119978848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACCG	25	3.8901362E-5	45.0	1
CGATAGA	20	7.0324785E-4	45.0	40
ATCGATA	20	7.0324785E-4	45.0	28
TAATCGA	20	7.0324785E-4	45.0	26
CGGACGT	20	7.0324785E-4	45.0	44
CCGATGA	1920	0.0	43.47656	18
GTCGAAT	435	0.0	43.448273	43
CGATGAA	2030	0.0	42.89409	19
GATGAAT	2105	0.0	42.755344	20
ATGAATG	2115	0.0	42.127663	21
AGTACGG	75	0.0	42.0	2
GACGTAG	65	0.0	41.53846	1
TCGAATG	455	0.0	41.53846	44
GGTCGAA	465	0.0	41.129032	42
CGTAAGG	115	0.0	41.08696	2
TATTACG	50	1.0804797E-9	40.5	1
CGGTCGA	275	0.0	40.090908	41
CGAATGC	395	0.0	39.873417	45
CCACCGA	2330	0.0	39.399143	15
AACCCGT	40	3.4571167E-7	39.375	38
>>END_MODULE