FastQCFastQC Report
Sat 18 Jun 2016
SRR3553921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832906
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.2073463271965864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12190.14635505087008618No Hit
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC9360.11237762724725239TruSeq Adapter, Index 20 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9060.10877578022009686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG253.889877E-545.01
CTATCGG207.032167E-445.02
GCACGTA253.889877E-545.033
TAAACCG253.889877E-545.011
TATATCG207.032167E-445.034
CTGCGTA207.032167E-445.041
ACGTCTT207.032167E-445.038
CGTGTAT253.889877E-545.037
ATGCGAA253.889877E-545.012
ACGGGTA1650.042.2727285
ATAAGCG650.041.5384641
TTATACG501.0804797E-940.51
CGCCGTT403.4566983E-739.37526
AACTACG403.4566983E-739.3751
CACGACC4500.039.027
ACAACGT356.2462186E-638.57142613
TCTTACG356.2462186E-638.5714261
CAATGCG356.2462186E-638.5714261
AATAGCG356.2462186E-638.5714261
AAATGCG356.2462186E-638.5714261
Produced by FastQC (version 0.11.5)