##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832906
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.943533844155283	33.0	31.0	34.0	30.0	34.0
2	32.03493191308503	33.0	31.0	34.0	30.0	34.0
3	32.02287052800676	33.0	31.0	34.0	30.0	34.0
4	35.68784952923859	37.0	35.0	37.0	33.0	37.0
5	35.588555011009646	37.0	35.0	37.0	33.0	37.0
6	35.555300358023594	37.0	35.0	37.0	33.0	37.0
7	35.869126888268305	37.0	35.0	37.0	35.0	37.0
8	35.78516303160261	37.0	35.0	37.0	35.0	37.0
9	37.505789368788314	39.0	37.0	39.0	35.0	39.0
10	36.94304039111256	39.0	37.0	39.0	33.0	39.0
11	36.90094200305917	39.0	37.0	39.0	33.0	39.0
12	36.775191918415764	39.0	35.0	39.0	33.0	39.0
13	36.73500851236514	39.0	35.0	39.0	33.0	39.0
14	37.64912126938694	40.0	36.0	41.0	33.0	41.0
15	37.80730958835691	40.0	37.0	41.0	33.0	41.0
16	37.89981942740237	40.0	37.0	41.0	33.0	41.0
17	37.846154307929105	40.0	36.0	41.0	33.0	41.0
18	37.81794224078107	40.0	36.0	41.0	33.0	41.0
19	37.766585905252214	40.0	36.0	41.0	33.0	41.0
20	37.701184767548796	40.0	36.0	41.0	33.0	41.0
21	37.53869944507544	39.0	35.0	41.0	32.0	41.0
22	37.589460275229136	39.0	35.0	41.0	33.0	41.0
23	37.54299044550045	39.0	35.0	41.0	33.0	41.0
24	37.48769609055524	39.0	35.0	41.0	33.0	41.0
25	37.358147257913856	39.0	35.0	41.0	32.0	41.0
26	37.329693867015	39.0	35.0	41.0	32.0	41.0
27	37.275633744984425	39.0	35.0	41.0	32.0	41.0
28	37.168179842623296	39.0	35.0	41.0	32.0	41.0
29	37.105152322110776	39.0	35.0	41.0	32.0	41.0
30	36.92466496819569	39.0	35.0	41.0	31.0	41.0
31	36.84623594979505	39.0	35.0	41.0	31.0	41.0
32	36.71051355134913	39.0	35.0	41.0	31.0	41.0
33	36.60565177823188	39.0	35.0	41.0	30.0	41.0
34	36.43582228967014	39.0	35.0	41.0	30.0	41.0
35	36.33483490333843	39.0	35.0	41.0	30.0	41.0
36	36.180033521189664	39.0	35.0	41.0	30.0	41.0
37	36.05934163038806	39.0	35.0	41.0	29.0	41.0
38	35.91325071496664	38.0	35.0	40.0	29.0	41.0
39	35.803582877299476	38.0	35.0	40.0	28.0	41.0
40	35.67068912938555	38.0	35.0	40.0	27.0	41.0
41	35.52288733662622	38.0	34.0	40.0	27.0	41.0
42	35.41838574821168	38.0	34.0	40.0	27.0	41.0
43	35.37465212160796	38.0	34.0	40.0	27.0	41.0
44	35.2609250023412	38.0	34.0	40.0	26.0	41.0
45	35.15551454786014	38.0	34.0	40.0	26.0	41.0
46	35.017518183324405	37.0	34.0	40.0	26.0	41.0
47	34.87806307074268	37.0	34.0	40.0	26.0	41.0
48	34.76901595137987	37.0	34.0	40.0	25.0	41.0
49	34.689508780102436	37.0	34.0	40.0	25.0	41.0
50	34.546186484429214	36.0	33.0	40.0	25.0	41.0
51	33.02919176953942	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	11.0
12	10.0
13	16.0
14	28.0
15	46.0
16	109.0
17	213.0
18	415.0
19	701.0
20	1336.0
21	1952.0
22	2716.0
23	3932.0
24	5161.0
25	6912.0
26	8663.0
27	10916.0
28	12879.0
29	15135.0
30	17665.0
31	21303.0
32	26845.0
33	36720.0
34	59147.0
35	67804.0
36	71232.0
37	100150.0
38	157931.0
39	202899.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.11252650359104	17.844150480366334	22.92575632784492	13.117566688197707
2	31.820517561405488	24.31210724859708	25.29036890117252	18.57700628882491
3	33.49141439730294	24.843619808237662	25.63698664675245	16.027979147706944
4	28.737696690863075	26.527243170297726	24.860428427697723	19.874631711141475
5	24.784789640127457	30.774901369422242	24.717915346989937	19.722393643460368
6	24.886001541590527	36.49715574146422	24.09263470307574	14.524208013869513
7	82.58675048564903	6.101288740866316	7.287136843773487	4.024823929711156
8	84.61194900745103	5.512386751926388	6.388716133633328	3.486948106989264
9	79.42492910364435	7.186885434850991	8.324108602891563	5.064076858613096
10	41.744086367489246	26.17702357769064	15.796860630131132	16.282029424688982
11	33.24372738340221	26.294083606073194	21.21439874367576	19.247790266848842
12	27.731580754610963	24.313788110543086	28.096447858461822	19.858183276384132
13	26.69232782570902	24.79907696666851	29.07278852595611	19.43580668166636
14	22.384518781231016	27.029580769018352	29.9719296055017	20.61397084424893
15	21.757797398505954	26.09214004941734	31.3282651343609	20.821797417715803
16	26.959464813556394	24.93690764624099	27.777804458126127	20.32582308207649
17	26.243297562990303	24.043169337236133	26.677200068194974	23.036333031578593
18	27.959217486727194	23.992623417288385	27.141358088427747	20.906801007556673
19	26.81395019365931	26.942175947826048	24.817686509642144	21.426187348872503
20	27.07820570388495	26.762323719603415	25.460256019286692	20.699214557224945
21	26.40406000196901	26.37608565672477	26.93221083771758	20.28764350358864
22	25.07053617094846	24.41007748773571	26.641661844193703	23.877724497122124
23	23.855513107121332	27.07436373372265	27.148321659346912	21.921801499809103
24	23.279097521208875	26.751878363224662	27.275586920973076	22.69343719459339
25	25.385697785824572	27.07820570388495	25.0946685460304	22.441427964260072
26	23.476598799864572	26.174262161636484	25.714186234701153	24.634952803797788
27	23.618871757437212	25.43816469085347	28.022249809702416	22.9207137420069
28	21.931886671485138	27.08276804345268	27.306322682271468	23.67902260279071
29	24.125531572590425	27.641054332661792	25.6905341058895	22.542879988858285
30	24.87171421504948	25.39614314220332	27.32085013194766	22.411292510799537
31	26.189750103853253	26.077132353470862	25.20860697365609	22.524510569019792
32	25.648872741942068	27.132833717130143	24.91073422451033	22.307559316417457
33	25.38437710858128	26.359397098832282	23.834862517498973	24.421363275087465
34	22.896341243789816	26.95442222771837	27.148441720914484	23.000794807577325
35	23.325561347859182	28.067392959109434	26.050838870172626	22.556206822858762
36	25.19888198668277	29.05201787476618	24.017356100208186	21.731744038342864
37	24.360852245031253	28.50393681880068	24.04749155366872	23.087719382499344
38	23.78731813674052	29.52566075883713	24.41512007357373	22.27190103084862
39	24.508768096279773	26.050958931740197	24.75765572585622	24.68261724612381
40	25.111717288625606	26.126957904013175	26.7218629713317	22.039461836029517
41	21.316090891409115	26.78621597155021	26.48702254516116	25.410670591879516
42	23.029369460659428	26.73687066727818	26.43359514759169	23.80016472447071
43	23.641203209005578	24.311146756056505	27.045428895937835	25.00222113900008
44	23.77867370387535	25.4103104071768	26.61885014635505	24.192165742592803
45	24.517172406009802	25.027073883487454	25.891397108437207	24.56435660206554
46	23.563043128516302	26.24846021039589	25.922613116005888	24.265883545081916
47	22.18173479360216	27.605756231795663	27.63877316287792	22.57373581172425
48	22.260615243496865	28.201381668519616	26.100784482282513	23.437218605701005
49	23.969931781017305	26.594357586570393	26.789937880144937	22.645772752267362
50	21.75899801418167	26.964987645664696	27.08661001361498	24.18940432653865
51	22.427500822421738	25.946025121682396	26.515957382945977	25.11051667294989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1573.0
1	1880.0
2	2187.0
3	2902.5
4	3618.0
5	2567.5
6	1517.0
7	1535.5
8	1554.0
9	1634.0
10	1714.0
11	1735.5
12	1757.0
13	1841.0
14	1925.0
15	2058.0
16	2191.0
17	2106.0
18	2021.0
19	2073.5
20	2126.0
21	2335.0
22	2544.0
23	3118.5
24	3693.0
25	4517.0
26	6173.5
27	7006.0
28	9210.5
29	11415.0
30	12802.5
31	14190.0
32	14814.5
33	15439.0
34	18631.0
35	21823.0
36	23482.5
37	25142.0
38	26274.0
39	27406.0
40	30279.0
41	33152.0
42	36209.0
43	39266.0
44	42140.0
45	45014.0
46	50090.0
47	55166.0
48	58849.5
49	62533.0
50	62690.5
51	62848.0
52	61126.0
53	59404.0
54	56721.5
55	54039.0
56	51673.0
57	49307.0
58	47046.0
59	44785.0
60	42602.5
61	40420.0
62	36720.5
63	33021.0
64	29604.5
65	26188.0
66	23399.5
67	20611.0
68	17986.0
69	15361.0
70	13571.5
71	11782.0
72	10105.0
73	8428.0
74	7161.0
75	4939.5
76	3985.0
77	3143.0
78	2301.0
79	1964.0
80	1627.0
81	1193.0
82	759.0
83	582.5
84	406.0
85	294.5
86	183.0
87	145.5
88	108.0
89	100.0
90	92.0
91	53.5
92	15.0
93	12.5
94	10.0
95	10.0
96	10.0
97	9.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	832906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.269118300967154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40178024548908	18.80067386825824
2	8.810261483419184	4.452550793739475
3	3.205884079430552	2.4302959218695337
4	1.7258228064815868	1.744400826539614
5	1.1392200766343281	1.439354344365485
6	0.8561415297681618	1.2980364958849602
7	0.6705130845553698	1.1860292119183222
8	0.559313402442811	1.1306685226915072
9	0.4808526639229138	1.0935650565002977
>10	6.823415228984672	41.525109042599546
>50	1.2436317103264067	20.413757849733162
>100	0.07647235728266162	3.1575236961466446
>500	0.005735426796199621	0.972233237223856
>1k	9.559044660332703E-4	0.3558011325293764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1727	0.2073463271965864	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1219	0.14635505087008618	No Hit
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	936	0.11237762724725239	TruSeq Adapter, Index 20 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	906	0.10877578022009686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.401231351437017E-4	0.0	0.0	0.04358234902858186	0.0
2	2.401231351437017E-4	0.0	0.0	0.2712190811448111	0.0
3	2.401231351437017E-4	0.0	0.0	0.3583837792019748	0.0
4	2.401231351437017E-4	0.0	0.0	0.507140061423498	0.0
5	2.401231351437017E-4	0.0	0.0	0.9773011600348659	0.0
6	2.401231351437017E-4	0.0	0.0	1.3715833479408241	0.0
7	2.401231351437017E-4	0.0	0.0	1.7138788770881708	0.0
8	2.401231351437017E-4	0.0	0.0	2.2356664497554344	0.0
9	2.401231351437017E-4	0.0	0.0	2.474348846088274	0.0
10	2.401231351437017E-4	0.0	0.0	3.066132312649927	0.0
11	2.401231351437017E-4	0.0	0.0	3.6765253221852165	0.0
12	2.401231351437017E-4	0.0	0.0	4.18342526047357	0.0
13	2.401231351437017E-4	0.0	0.0	4.379605861885975	0.0
14	2.401231351437017E-4	0.0	0.0	4.467250806213427	0.0
15	2.401231351437017E-4	0.0	0.0	4.621289797408111	0.0
16	2.401231351437017E-4	0.0	0.0	4.920002977526876	0.0
17	2.401231351437017E-4	0.0	0.0	5.273224109323261	0.0
18	2.401231351437017E-4	0.0	0.0	5.72789726571786	0.0
19	2.401231351437017E-4	0.0	0.0	5.964058369131691	0.0
20	2.401231351437017E-4	0.0	0.0	6.221470370005739	0.0
21	2.401231351437017E-4	0.0	0.0	6.508537578070034	0.0
22	2.401231351437017E-4	0.0	0.0	6.824299500784002	0.0
23	2.401231351437017E-4	0.0	0.0	7.116169171551172	0.0
24	2.401231351437017E-4	0.0	0.0	7.376822834749659	0.0
25	2.401231351437017E-4	0.0	0.0	7.606020367244323	0.0
26	2.401231351437017E-4	0.0	0.0	7.824172235522376	0.0
27	2.401231351437017E-4	0.0	0.0	8.1034354416945	0.0
28	2.401231351437017E-4	0.0	0.0	8.346560116027499	0.0
29	2.401231351437017E-4	0.0	0.0	8.629185046091635	0.0
30	2.401231351437017E-4	0.0	0.0	8.968959282319974	0.0
31	4.802462702874034E-4	0.0	0.0	9.225290729085875	0.0
32	4.802462702874034E-4	0.0	0.0	9.487865377365512	0.0
33	4.802462702874034E-4	0.0	0.0	9.763526736510483	0.0
34	4.802462702874034E-4	0.0	0.0	10.049753513601775	0.0
35	7.20369405431105E-4	0.0	0.0	10.345945400801531	0.0
36	7.20369405431105E-4	0.0	0.0	10.596633953891555	0.0
37	7.20369405431105E-4	0.0	0.0	10.859208602171194	0.0
38	7.20369405431105E-4	0.0	0.0	11.190818651804646	0.0
39	8.40430973002956E-4	0.0	0.0	11.748024386905605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	25	3.889877E-5	45.0	1
CTATCGG	20	7.032167E-4	45.0	2
GCACGTA	25	3.889877E-5	45.0	33
TAAACCG	25	3.889877E-5	45.0	11
TATATCG	20	7.032167E-4	45.0	34
CTGCGTA	20	7.032167E-4	45.0	41
ACGTCTT	20	7.032167E-4	45.0	38
CGTGTAT	25	3.889877E-5	45.0	37
ATGCGAA	25	3.889877E-5	45.0	12
ACGGGTA	165	0.0	42.272728	5
ATAAGCG	65	0.0	41.538464	1
TTATACG	50	1.0804797E-9	40.5	1
CGCCGTT	40	3.4566983E-7	39.375	26
AACTACG	40	3.4566983E-7	39.375	1
CACGACC	450	0.0	39.0	27
ACAACGT	35	6.2462186E-6	38.571426	13
TCTTACG	35	6.2462186E-6	38.571426	1
CAATGCG	35	6.2462186E-6	38.571426	1
AATAGCG	35	6.2462186E-6	38.571426	1
AAATGCG	35	6.2462186E-6	38.571426	1
>>END_MODULE