FastQCFastQC Report
Sat 18 Jun 2016
SRR3553920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812681
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.24499157726094248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13540.16660903847881273No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC9270.11406689709738506No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT8440.10385378764853614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG253.889793E-545.024
ACGTTAC207.032066E-445.043
ATTACGT207.032066E-445.018
CACGAAC207.032066E-445.041
ATACTCG406.8102963E-945.044
ATCGTAA207.032066E-445.044
CCGTAGT207.032066E-445.027
GTCACGA253.889793E-545.08
GTCGTTA207.032066E-445.019
TTAACGG406.8102963E-945.02
TCGTAAG207.032066E-445.045
ATAACGC351.2113378E-745.011
GTGCGAT207.032066E-445.08
CACGGGC2000.041.6254
GCCGATT650.041.538469
CGCATCG403.4565528E-739.37521
CGCATAC403.4565528E-739.37536
CGGACGA403.4565528E-739.37532
ACGACCA2900.038.79310228
TACGCAT1050.038.5714316