##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	812681
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04687571137999	33.0	31.0	34.0	30.0	34.0
2	32.14482681396513	33.0	31.0	34.0	30.0	34.0
3	32.126713925882356	33.0	31.0	34.0	30.0	34.0
4	35.767181710905014	37.0	35.0	37.0	35.0	37.0
5	35.69288441590243	37.0	35.0	37.0	33.0	37.0
6	35.663699532781	37.0	35.0	37.0	33.0	37.0
7	35.965938664740534	37.0	35.0	37.0	35.0	37.0
8	35.91136743691559	37.0	35.0	37.0	35.0	37.0
9	37.60404266864858	39.0	38.0	39.0	35.0	39.0
10	37.03923802820541	39.0	37.0	39.0	33.0	39.0
11	36.96917240590096	39.0	37.0	39.0	33.0	39.0
12	36.958953143976544	39.0	37.0	39.0	33.0	39.0
13	36.94550629336726	39.0	37.0	39.0	33.0	39.0
14	38.041988184785914	40.0	37.0	41.0	33.0	41.0
15	38.17556581241594	40.0	37.0	41.0	33.0	41.0
16	38.21472878041938	40.0	37.0	41.0	33.0	41.0
17	38.162719443422446	40.0	37.0	41.0	33.0	41.0
18	38.12295722429834	40.0	37.0	41.0	33.0	41.0
19	38.05100279199341	40.0	37.0	41.0	34.0	41.0
20	38.017278612395266	40.0	37.0	41.0	33.0	41.0
21	37.88089299491436	40.0	36.0	41.0	33.0	41.0
22	37.9075356751296	40.0	36.0	41.0	33.0	41.0
23	37.83975385175733	40.0	36.0	41.0	33.0	41.0
24	37.783733100687726	40.0	36.0	41.0	33.0	41.0
25	37.65214887514289	40.0	36.0	41.0	33.0	41.0
26	37.64994505839314	40.0	36.0	41.0	33.0	41.0
27	37.623717055031435	40.0	36.0	41.0	33.0	41.0
28	37.50278399519615	40.0	36.0	41.0	33.0	41.0
29	37.469672602164934	40.0	36.0	41.0	33.0	41.0
30	37.31296658836616	40.0	35.0	41.0	32.0	41.0
31	37.224278653001605	39.0	35.0	41.0	32.0	41.0
32	37.093467178388565	39.0	35.0	41.0	31.0	41.0
33	37.00148151611764	39.0	35.0	41.0	31.0	41.0
34	36.84367913117201	39.0	35.0	41.0	31.0	41.0
35	36.76680148791469	39.0	35.0	41.0	31.0	41.0
36	36.62380442018455	39.0	35.0	41.0	30.0	41.0
37	36.57385739299922	39.0	35.0	41.0	30.0	41.0
38	36.474381706967435	39.0	35.0	41.0	30.0	41.0
39	36.362279910567615	39.0	35.0	41.0	30.0	41.0
40	36.23123710287308	39.0	35.0	41.0	30.0	41.0
41	36.138422086895105	39.0	35.0	41.0	29.0	41.0
42	36.030176662183564	39.0	35.0	41.0	29.0	41.0
43	35.99879165379774	39.0	35.0	41.0	29.0	41.0
44	35.8907443387996	38.0	35.0	40.0	29.0	41.0
45	35.8243861982746	38.0	35.0	40.0	29.0	41.0
46	35.66387672407747	38.0	35.0	40.0	28.0	41.0
47	35.57241402223013	38.0	34.0	40.0	28.0	41.0
48	35.49319966875071	38.0	34.0	40.0	28.0	41.0
49	35.41126715156378	38.0	34.0	40.0	27.0	41.0
50	35.27607634483887	38.0	34.0	40.0	27.0	41.0
51	33.813672277314225	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	6.0
11	6.0
12	13.0
13	23.0
14	20.0
15	51.0
16	94.0
17	171.0
18	317.0
19	583.0
20	893.0
21	1453.0
22	2107.0
23	2993.0
24	4258.0
25	5822.0
26	7399.0
27	8966.0
28	10394.0
29	11960.0
30	14605.0
31	18246.0
32	22883.0
33	31264.0
34	52173.0
35	62920.0
36	65650.0
37	93649.0
38	158716.0
39	234971.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24191287848492	17.931390053415793	24.072298970936938	15.754398097162357
2	30.21899121549538	25.19463356470743	27.287951853187163	17.29842336661002
3	32.4753501066224	24.295387735162997	27.02548724530289	16.203774912911708
4	27.600743711247095	27.114452042068166	26.70408192144273	18.580722325242007
5	24.43295708894388	30.278916327562722	24.83163750598328	20.456489077510117
6	24.230171494104084	35.410204003785	25.503980036447267	14.855644465663648
7	83.02950358135604	5.86306312070788	7.52423152503873	3.5832017728973606
8	85.54721963476443	4.68781723702166	6.708413264245134	3.056549863968765
9	79.2810463146056	7.374603319137521	8.683480972238799	4.660869394018071
10	38.84291622420113	29.53323628828532	16.733503059626102	14.89034442788745
11	28.342609215670112	26.787878638727864	25.857378233279725	19.0121339123223
12	26.300233424923185	23.697490159115322	29.896232347009466	20.106044068952027
13	24.137023013950124	25.206692416827757	30.567098283336264	20.08918628588585
14	21.517298915564655	27.849549823362423	30.124366141204234	20.50878511986868
15	20.789830203979175	26.477424721385145	32.45590828381616	20.276836790819523
16	23.222765144010996	25.065185478681055	31.42463032850528	20.287419048802665
17	23.863360900525542	24.398134077208645	28.997847864045056	22.740657158220753
18	24.768143958084416	25.23733174517431	29.248253619809	20.746270676932276
19	24.487960220553944	27.096979011444834	27.780149898914825	20.634910869086394
20	25.134708452640087	26.197364033366107	27.880804399266136	20.787123114727674
21	24.751532274041104	26.42192939172935	28.877136293330345	19.949402040899198
22	23.535064804025197	24.575817571716332	28.821025716117394	23.068091908141078
23	22.641356202495196	26.753793924061224	29.106623632150868	21.49822624129271
24	21.560366244565827	26.464873671219085	29.997255995895067	21.977504088320018
25	23.092824859938894	26.486776484253973	27.98638088007472	22.43401777573242
26	21.093762497216005	27.751110214216894	28.120012649489773	23.035114639077324
27	21.642932466736642	26.628529521423534	28.815488488102957	22.913049523736866
28	20.14701955625885	27.39143649230141	30.380063025959757	22.081480925479983
29	22.09723126294327	26.8022754315654	29.002892894013765	22.097600411477565
30	22.380122089725244	26.301463920037506	29.308301781387776	22.010112208849474
31	23.92919238914162	26.770774756638826	27.788148117157903	21.511884737061653
32	23.418906065233468	27.129833230997157	27.917596203184274	21.5336645005851
33	23.221903797430972	27.265802941129426	26.50486476243446	23.007428499005144
34	21.329894509653847	27.505749488421653	29.169255833469716	21.99510016845478
35	22.13636100757862	27.649717416797	28.073007736122786	22.1409138395016
36	23.346306853488638	28.548717147318563	26.8832420100876	21.221733989105196
37	23.165054923149427	28.80146084379972	26.20794629134925	21.825537941701604
38	22.60013461616551	28.387645336854188	27.30087205188752	21.711347995092787
39	23.633381363659296	26.25322851155619	27.804390652666918	22.3089994721176
40	22.81940884553718	26.74148897291803	28.875905798216028	21.56319638332876
41	20.748977766183778	27.579948343815104	28.555730969470183	23.115342920530935
42	21.977627137831448	26.803628976191153	28.50035868932582	22.718385196651578
43	22.51806059204042	26.41996059954644	27.83306118883055	23.228917619582592
44	22.610839923660084	26.43989462039841	28.390475475617123	22.558789980324384
45	22.639264360800855	26.952518885023768	27.401526552238824	23.006690201936554
46	22.681347293710573	27.456406634337462	27.920057193412912	21.942188878539056
47	21.364963620411945	27.830108000556187	29.107977176776618	21.696951202255253
48	22.207606674697697	27.609111078024462	27.849180674828126	22.334101572449708
49	23.00582885535653	27.31317700303071	28.16160338435376	21.519390757259
50	21.45651245691729	26.77729638074472	28.563975286736127	23.202215875601865
51	21.325833875776596	26.386614181948392	28.68333331282508	23.60421862944993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1748.0
1	2161.0
2	2574.0
3	3432.5
4	4291.0
5	3105.0
6	1919.0
7	1968.5
8	2018.0
9	2009.5
10	2001.0
11	2027.0
12	2053.0
13	2385.0
14	2717.0
15	2835.0
16	2953.0
17	3007.0
18	3061.0
19	2891.5
20	2722.0
21	3211.5
22	3701.0
23	3888.5
24	4076.0
25	5172.0
26	7015.5
27	7763.0
28	9764.5
29	11766.0
30	14897.5
31	18029.0
32	20318.0
33	22607.0
34	25104.0
35	27601.0
36	29857.0
37	32113.0
38	31805.0
39	31497.0
40	35069.0
41	38641.0
42	42040.0
43	45439.0
44	48020.0
45	50601.0
46	55019.5
47	59438.0
48	61987.5
49	64537.0
50	63838.0
51	63139.0
52	58381.5
53	53624.0
54	49182.0
55	44740.0
56	42148.0
57	39556.0
58	36743.5
59	33931.0
60	32544.5
61	31158.0
62	27921.5
63	24685.0
64	22261.0
65	19837.0
66	16948.5
67	14060.0
68	12398.5
69	10737.0
70	9507.0
71	8277.0
72	7448.0
73	6619.0
74	5614.5
75	3638.5
76	2667.0
77	1994.5
78	1322.0
79	977.0
80	632.0
81	511.5
82	391.0
83	361.5
84	332.0
85	213.5
86	95.0
87	76.5
88	58.0
89	41.0
90	24.0
91	17.5
92	11.0
93	12.5
94	14.0
95	19.0
96	24.0
97	14.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	812681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.800977621610986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30971324982417	18.429535353772188
2	8.286870714327048	4.110449902784172
3	3.098072006867035	2.305056435373463
4	1.7320764661031516	1.718287586989732
5	1.1631778876260124	1.4423974380482734
6	0.8937597129168907	1.329966878348956
7	0.7209778837655858	1.2516669452662716
8	0.6406282451294456	1.2710565416981838
9	0.5394133855081389	1.2040181372506293
>10	7.333140028856411	43.58003278460363
>50	1.1948291098749986	19.189612114976274
>100	0.08334856363767586	3.195504266007649
>500	0.002994559172611109	0.5583715426077046
>1k	9.981863908703698E-4	0.414044072272838
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1991	0.24499157726094248	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1354	0.16660903847881273	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	927	0.11406689709738506	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	844	0.10385378764853614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2304951143191485E-4	0.0	0.0	0.058817666464455305	0.0
2	1.2304951143191485E-4	0.0	0.0	0.2969184710852106	0.0
3	1.2304951143191485E-4	0.0	0.0	0.3999109121537233	0.0
4	1.2304951143191485E-4	0.0	0.0	0.5251753147914127	0.0
5	1.2304951143191485E-4	0.0	0.0	0.9531415155516125	0.0
6	1.2304951143191485E-4	0.0	0.0	1.3058014153154804	0.0
7	1.2304951143191485E-4	0.0	0.0	1.6002588961720527	0.0
8	1.2304951143191485E-4	0.0	0.0	2.0651399503618273	0.0
9	1.2304951143191485E-4	0.0	0.0	2.2903205562822313	0.0
10	1.2304951143191485E-4	0.0	0.0	2.794823553153082	0.0
11	1.2304951143191485E-4	0.0	0.0	3.4538767363824183	0.0
12	1.2304951143191485E-4	0.0	0.0	3.9577644856961096	0.0
13	1.2304951143191485E-4	0.0	0.0	4.160057882490178	0.0
14	1.2304951143191485E-4	0.0	0.0	4.240655312478082	0.0
15	1.2304951143191485E-4	0.0	0.0	4.382654448670512	0.0
16	1.2304951143191485E-4	0.0	0.0	4.684494900212998	0.0
17	1.2304951143191485E-4	0.0	0.0	5.069270722460597	0.0
18	1.2304951143191485E-4	0.0	0.0	5.5140947062869685	0.0
19	1.2304951143191485E-4	0.0	0.0	5.75773273892216	0.0
20	1.2304951143191485E-4	0.0	0.0	5.998417583282985	0.0
21	1.2304951143191485E-4	0.0	0.0	6.280570112996366	0.0
22	1.2304951143191485E-4	0.0	0.0	6.582656663561718	0.0
23	1.2304951143191485E-4	0.0	0.0	6.8870811548442745	0.0
24	1.2304951143191485E-4	0.0	0.0	7.13293407868524	0.0
25	1.2304951143191485E-4	0.0	0.0	7.356884189491326	0.0
26	2.460990228638297E-4	0.0	0.0	7.56988289377997	0.0
27	2.460990228638297E-4	0.0	0.0	7.802323420874857	0.0
28	2.460990228638297E-4	0.0	0.0	8.044607908884299	0.0
29	2.460990228638297E-4	0.0	0.0	8.276310138910594	0.0
30	2.460990228638297E-4	0.0	0.0	8.561785005432636	0.0
31	2.460990228638297E-4	0.0	0.0	8.78696561135304	0.0
32	2.460990228638297E-4	0.0	0.0	9.027527406202434	0.0
33	2.460990228638297E-4	0.0	0.0	9.267597003006099	0.0
34	2.460990228638297E-4	0.0	0.0	9.516033966587136	0.0
35	2.460990228638297E-4	0.0	0.0	9.787850337340236	0.0
36	2.460990228638297E-4	0.0	0.0	10.039732687241365	0.0
37	2.460990228638297E-4	0.0	0.0	10.291491987631064	0.0
38	2.460990228638297E-4	0.0	0.0	10.568107289330008	0.0
39	3.6914853429574455E-4	0.0	0.0	10.889512613190169	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGCG	25	3.889793E-5	45.0	24
ACGTTAC	20	7.032066E-4	45.0	43
ATTACGT	20	7.032066E-4	45.0	18
CACGAAC	20	7.032066E-4	45.0	41
ATACTCG	40	6.8102963E-9	45.0	44
ATCGTAA	20	7.032066E-4	45.0	44
CCGTAGT	20	7.032066E-4	45.0	27
GTCACGA	25	3.889793E-5	45.0	8
GTCGTTA	20	7.032066E-4	45.0	19
TTAACGG	40	6.8102963E-9	45.0	2
TCGTAAG	20	7.032066E-4	45.0	45
ATAACGC	35	1.2113378E-7	45.0	11
GTGCGAT	20	7.032066E-4	45.0	8
CACGGGC	200	0.0	41.625	4
GCCGATT	65	0.0	41.53846	9
CGCATCG	40	3.4565528E-7	39.375	21
CGCATAC	40	3.4565528E-7	39.375	36
CGGACGA	40	3.4565528E-7	39.375	32
ACGACCA	290	0.0	38.793102	28
TACGCAT	105	0.0	38.57143	16
>>END_MODULE