##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	601470
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.999750611003044	33.0	31.0	34.0	30.0	34.0
2	32.09593495934959	33.0	31.0	34.0	30.0	34.0
3	32.071084177099436	33.0	31.0	34.0	30.0	34.0
4	35.726510050376575	37.0	35.0	37.0	33.0	37.0
5	35.64772141586447	37.0	35.0	37.0	33.0	37.0
6	35.61336226245698	37.0	35.0	37.0	33.0	37.0
7	35.899369877134355	37.0	35.0	37.0	35.0	37.0
8	35.81481370641927	37.0	35.0	37.0	35.0	37.0
9	37.52120804030126	39.0	37.0	39.0	35.0	39.0
10	36.99036194656425	39.0	37.0	39.0	33.0	39.0
11	36.94756346949973	39.0	37.0	39.0	33.0	39.0
12	36.878125259780205	39.0	37.0	39.0	33.0	39.0
13	36.857711939082584	39.0	37.0	39.0	33.0	39.0
14	37.919917867890334	40.0	37.0	41.0	33.0	41.0
15	38.05839360234093	40.0	37.0	41.0	33.0	41.0
16	38.097309924019484	40.0	37.0	41.0	33.0	41.0
17	38.0402846359752	40.0	37.0	41.0	33.0	41.0
18	37.97749181172793	40.0	37.0	41.0	33.0	41.0
19	37.88995959898249	40.0	36.0	41.0	33.0	41.0
20	37.84461070377575	40.0	36.0	41.0	33.0	41.0
21	37.65823066819625	40.0	36.0	41.0	33.0	41.0
22	37.67501953547143	40.0	36.0	41.0	33.0	41.0
23	37.60740685320964	39.0	35.0	41.0	33.0	41.0
24	37.567772291219846	39.0	35.0	41.0	33.0	41.0
25	37.43707250569438	39.0	35.0	41.0	33.0	41.0
26	37.401970173075966	39.0	35.0	41.0	33.0	41.0
27	37.37247909288909	39.0	35.0	41.0	33.0	41.0
28	37.24373119191315	39.0	35.0	41.0	32.0	41.0
29	37.20516235223702	39.0	35.0	41.0	32.0	41.0
30	37.03607993748649	39.0	35.0	41.0	31.0	41.0
31	36.94640464196053	39.0	35.0	41.0	31.0	41.0
32	36.784918616057325	39.0	35.0	41.0	31.0	41.0
33	36.674815036493925	39.0	35.0	41.0	31.0	41.0
34	36.50999218581143	39.0	35.0	41.0	30.0	41.0
35	36.423035230352305	39.0	35.0	41.0	30.0	41.0
36	36.224343691289675	39.0	35.0	41.0	30.0	41.0
37	36.19917867890335	39.0	35.0	41.0	30.0	41.0
38	36.05911184265217	39.0	35.0	41.0	29.0	41.0
39	35.965745589971235	39.0	35.0	41.0	29.0	41.0
40	35.836227908291356	38.0	35.0	40.0	28.0	41.0
41	35.69414102116481	38.0	35.0	40.0	28.0	41.0
42	35.5785708347881	38.0	35.0	40.0	27.0	41.0
43	35.483473822468284	38.0	34.0	40.0	27.0	41.0
44	35.36445042978037	38.0	34.0	40.0	27.0	41.0
45	35.2726968926131	38.0	34.0	40.0	26.0	41.0
46	35.13276971420021	38.0	34.0	40.0	26.0	41.0
47	35.033940179892596	38.0	34.0	40.0	26.0	41.0
48	34.952346750461366	37.0	34.0	40.0	26.0	41.0
49	34.84271534739887	37.0	34.0	40.0	26.0	41.0
50	34.708731940080135	37.0	34.0	40.0	25.0	41.0
51	33.22616423096746	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	2.0
11	5.0
12	9.0
13	18.0
14	20.0
15	42.0
16	93.0
17	143.0
18	307.0
19	551.0
20	895.0
21	1342.0
22	1979.0
23	2680.0
24	3739.0
25	5053.0
26	6348.0
27	7677.0
28	8416.0
29	9883.0
30	11697.0
31	14511.0
32	18496.0
33	25080.0
34	40844.0
35	50276.0
36	50524.0
37	70215.0
38	113792.0
39	156775.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88220526377043	18.858297171928776	22.960413653216285	13.299083911084509
2	32.794486840573924	25.005070909604804	24.624835818910338	17.575606430910934
3	32.95459457662061	24.20070826475136	26.361082015728133	16.483615142899897
4	27.606696925864966	26.842236520524715	25.70585399105525	19.845212562555073
5	24.452591151678387	30.51274377774453	24.822850682494554	20.21181438808253
6	24.611036294412024	35.80760470181389	24.774801735747417	14.806557268026669
7	81.69484762332286	6.398490365271751	7.856085922822419	4.050576088582972
8	83.26317189552263	5.66611801087336	7.284652601127238	3.786057492476765
9	77.98709827589073	7.8595773687798225	8.752722496550119	5.400601858779324
10	39.50454719271119	26.739820772440854	17.324388581309126	16.43124345353883
11	31.14153656873992	27.926746138627028	22.46645717990922	18.46526011272383
12	26.93002144745374	24.99958435167174	28.140721898016523	19.929672302858
13	25.506342793489285	26.31519444028796	28.18710825145061	19.99135451477214
14	22.286730842768552	28.732438858130916	28.540243071142367	20.44058722795817
15	21.0136831429664	27.37343508404409	30.742347914276692	20.870533858712818
16	24.424493324687848	26.220426621444126	29.614777129366388	19.740302924501638
17	24.46705571350192	24.536385854656093	28.38146540974612	22.615093022095863
18	25.795467770628626	24.586429913378886	28.114951701664253	21.503150614328227
19	25.72863151944403	26.508387783264336	26.307380251716626	21.45560044557501
20	26.874158312135272	26.420104078341396	25.83088100819658	20.874856601326748
21	26.078939930503598	27.272515669941978	26.835586147272515	19.81295825228191
22	24.815867790579745	24.857266364074686	27.185562039669474	23.141303805676095
23	22.488735930304088	27.92209087735049	27.588408399421414	22.000764792924002
24	22.268109797662394	26.514040600528705	28.31180274992934	22.906046851879562
25	23.161088666101385	27.6613962458643	26.94614860259032	22.231366485443996
26	22.604618684223652	27.942540775101005	26.733669177182566	22.719171363492777
27	22.765890235589474	27.27201689194806	27.56047683176218	22.401616040700283
28	21.934261060402015	27.481004871398408	27.64327397875206	22.94146008944752
29	23.25635526293913	27.580594210850084	27.376760270670193	21.786290255540592
30	23.96312368031656	26.41062729645701	28.103978585798128	21.5222704374283
31	25.346567576105212	27.547674863251697	25.602773205646166	21.502984354996922
32	24.491994613197665	28.118276888290357	25.80427951518779	21.58544898332419
33	24.434136365903537	27.628144379603302	24.8913495269922	23.046369727500956
34	23.065822069263636	28.042130114552677	27.21615375662959	21.675894059554093
35	23.38869769065789	27.981112939963754	26.299067285151377	22.331122084226976
36	24.36297737210501	28.618717475518313	25.646166891116764	21.372138261259913
37	24.391906495752075	28.019186326832596	25.538098325768534	22.0508088516468
38	23.996375546577553	28.76003790712754	25.061432822917183	22.182153723377724
39	24.202869636058324	26.676309707882357	26.18534590253878	22.93547475352054
40	24.535887076662178	26.128983989226395	27.465210234924438	21.86991869918699
41	22.12911699669144	27.198197748848653	27.73804179759589	22.934643456864016
42	24.423662028031323	27.010158445142736	26.07162451992618	22.494555006899763
43	24.388248790463365	26.11485194606547	26.633248541074366	22.863650722396795
44	23.655876436064975	27.294960679668144	26.50173741001214	22.54742547425474
45	23.701431492842538	26.87099938484047	26.707732721498996	22.719836400817996
46	23.396345619897918	27.55033501255258	26.24237285317638	22.81094651437312
47	22.733469665985005	27.697142002094864	27.258716145443664	22.310672186476467
48	23.096580045555058	26.6678304819858	27.30277486823948	22.93281460421966
49	24.10328029660665	26.780221789947962	26.893111875904037	22.223386037541356
50	21.8266912730477	27.453239563070476	27.53370907942208	23.18636008445974
51	22.623904766655027	26.482617586912067	26.73832443845911	24.155153207973797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1728.0
1	2007.0
2	2286.0
3	2891.5
4	3497.0
5	2560.0
6	1623.0
7	1629.0
8	1635.0
9	1820.0
10	2005.0
11	1987.5
12	1970.0
13	2091.0
14	2212.0
15	2202.0
16	2192.0
17	2180.5
18	2169.0
19	2187.5
20	2206.0
21	2467.0
22	2728.0
23	2967.5
24	3207.0
25	3611.0
26	4752.5
27	5490.0
28	6762.5
29	8035.0
30	9167.0
31	10299.0
32	11868.0
33	13437.0
34	15366.5
35	17296.0
36	18103.0
37	18910.0
38	20278.5
39	21647.0
40	23341.5
41	25036.0
42	28068.5
43	31101.0
44	32816.5
45	34532.0
46	37632.0
47	40732.0
48	43046.0
49	45360.0
50	45965.5
51	46571.0
52	43878.0
53	41185.0
54	38204.5
55	35224.0
56	32870.0
57	30516.0
58	29306.0
59	28096.0
60	26726.0
61	25356.0
62	23106.5
63	20857.0
64	18833.0
65	16809.0
66	15070.5
67	13332.0
68	12102.0
69	10872.0
70	9829.5
71	8787.0
72	7668.0
73	6549.0
74	5646.0
75	3962.0
76	3181.0
77	2482.5
78	1784.0
79	1484.5
80	1185.0
81	864.5
82	544.0
83	418.0
84	292.0
85	215.0
86	138.0
87	85.5
88	33.0
89	34.0
90	35.0
91	30.5
92	26.0
93	15.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	601470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.785507247835078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15410684226751	20.130408735281282
2	8.093228227636862	4.335624466995012
3	2.9280334143850544	2.352865807287425
4	1.6513574784264924	1.7692979082863787
5	1.113506473065056	1.4912917852397667
6	0.8981004232653075	1.4433645237993988
7	0.6907086175775031	1.2950686477585052
8	0.6201550730137697	1.3288934562393617
9	0.5115468689222179	1.2331838128611068
>10	7.474820712842681	47.81217416921075
>50	0.8152706896060463	13.820264998966753
>100	0.0454311147643064	1.9690368630472497
>500	0.0024893761514688437	0.5050949623292555
>1k	0.0012446880757344219	0.5134298626977252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1578	0.26235722479924184	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1502	0.2497215156200642	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	949	0.15778010540841605	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	802	0.13333998370658554	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	748	0.12436197981611717	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.32518662609939E-4	0.0	0.0	0.04272864814537716	0.0
2	3.32518662609939E-4	0.0	0.0	0.23725206577219146	0.0
3	3.32518662609939E-4	0.0	0.0	0.3255357706951303	0.0
4	3.32518662609939E-4	0.0	0.0	0.4518928624869071	0.0
5	3.32518662609939E-4	0.0	0.0	0.8349543618135568	0.0
6	3.32518662609939E-4	0.0	0.0	1.1693018770678505	0.0
7	3.32518662609939E-4	0.0	0.0	1.4670723394350509	0.0
8	3.32518662609939E-4	0.0	0.0	1.9191314612532628	0.0
9	3.32518662609939E-4	0.0	0.0	2.141918965201922	0.0
10	3.32518662609939E-4	0.0	0.0	2.6671321927943206	0.0
11	3.32518662609939E-4	0.0	0.0	3.3960131012353068	0.0
12	3.32518662609939E-4	0.0	0.0	3.92372021879728	0.0
13	3.32518662609939E-4	0.0	0.0	4.132874457578931	0.0
14	3.32518662609939E-4	0.0	0.0	4.225647164447104	0.0
15	4.987779939149084E-4	0.0	0.0	4.379437045904202	0.0
16	4.987779939149084E-4	0.0	0.0	4.702312667298452	0.0
17	4.987779939149084E-4	0.0	0.0	5.077061200059854	0.0
18	4.987779939149084E-4	0.0	0.0	5.515985834704972	0.0
19	4.987779939149084E-4	0.0	0.0	5.7379420419971074	0.0
20	4.987779939149084E-4	0.0	0.0	5.971037624486675	0.0
21	4.987779939149084E-4	0.0	0.0	6.258500008312967	0.0
22	6.65037325219878E-4	0.0	0.0	6.531331570984421	0.0
23	6.65037325219878E-4	0.0	0.0	6.821620363442898	0.0
24	6.65037325219878E-4	0.0	0.0	7.053718389944636	0.0
25	6.65037325219878E-4	0.0	0.0	7.261542554075848	0.0
26	6.65037325219878E-4	0.0	0.0	7.457063527690492	0.0
27	6.65037325219878E-4	0.0	0.0	7.6748632517000015	0.0
28	6.65037325219878E-4	0.0	0.0	7.886843899113837	0.0
29	6.65037325219878E-4	0.0	0.0	8.112956589688597	0.0
30	6.65037325219878E-4	0.0	0.0	8.375480073819142	0.0
31	8.312966565248474E-4	0.0	0.0	8.606081766339136	0.0
32	8.312966565248474E-4	0.0	0.0	8.828869270287795	0.0
33	8.312966565248474E-4	0.0	0.0	9.056810813506909	0.0
34	8.312966565248474E-4	0.0	0.0	9.288576321346035	0.0
35	8.312966565248474E-4	0.0	0.0	9.536136465659135	0.0
36	8.312966565248474E-4	0.0	0.0	9.75343741167473	0.0
37	8.312966565248474E-4	0.0	0.0	9.988195587477348	0.0
38	8.312966565248474E-4	0.0	0.0	10.202670124860758	0.0
39	8.312966565248474E-4	0.0	0.0	10.459873310389545	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	3.8885795E-5	45.000004	1
AAGCGTA	25	3.8885795E-5	45.000004	22
ATATCGT	25	3.8885795E-5	45.000004	26
AATTGCG	30	2.1637698E-6	45.000004	1
TACGAAT	50	2.1827873E-11	45.000004	12
ATGCGAG	25	3.8885795E-5	45.000004	1
TTACGCG	25	3.8885795E-5	45.000004	1
CCAACCG	25	3.8885795E-5	45.000004	33
GCTAACG	25	3.8885795E-5	45.000004	1
AACGTAG	55	1.8189894E-12	45.0	1
ATTCGAT	20	7.030602E-4	45.0	31
AACGCCC	20	7.030602E-4	45.0	24
GTCGTAC	20	7.030602E-4	45.0	27
TTGCGCA	20	7.030602E-4	45.0	1
TCGTACT	20	7.030602E-4	45.0	28
TTACGGA	20	7.030602E-4	45.0	44
TATACGG	35	1.2106284E-7	45.0	2
TATCGTT	20	7.030602E-4	45.0	12
TAATGCG	20	7.030602E-4	45.0	1
TACACGT	20	7.030602E-4	45.0	19
>>END_MODULE