FastQCFastQC Report
Sat 18 Jun 2016
SRR3553909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835607
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28790.3445399571808278No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.33269228237676324No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12020.14384752640894582No Hit
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC11370.13606875002243876No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC11340.13570972957383076No Hit
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC11210.13415397429652937No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT8860.10603070582223462No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.03218E-445.01
ACCGGAT207.03218E-445.035
CACGGAT207.03218E-445.04
ATAATCG207.03218E-445.038
TATATCG207.03218E-445.033
TTCGTGC207.03218E-445.036
CGACATA207.03218E-445.032
TGACGGA207.03218E-445.029
ATATCGC207.03218E-445.034
AACGCCG207.03218E-445.013
CGTACTC253.8898892E-545.045
ACGTCGC207.03218E-445.020
CGCGTAC207.03218E-445.043
ATACCGG207.03218E-445.02
GCCCGTA207.03218E-445.09
GTCGGTT207.03218E-445.012
CAATGCG302.1647884E-644.9999961
GTACGAT302.1647884E-644.99999610
CCCGCTA800.042.187523
GTTACGG700.041.7857172