##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835607
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.125907274592	33.0	31.0	34.0	30.0	34.0
2	32.28656413840478	34.0	31.0	34.0	30.0	34.0
3	32.29989337092676	34.0	31.0	34.0	30.0	34.0
4	35.83218785864647	37.0	35.0	37.0	35.0	37.0
5	35.7648272453438	37.0	35.0	37.0	35.0	37.0
6	35.74551194520869	37.0	35.0	37.0	35.0	37.0
7	35.925439829967914	37.0	35.0	37.0	35.0	37.0
8	35.84926765812158	37.0	35.0	37.0	35.0	37.0
9	37.64334310267865	39.0	38.0	39.0	35.0	39.0
10	37.04406377639249	39.0	37.0	39.0	33.0	39.0
11	36.99930589379936	39.0	37.0	39.0	33.0	39.0
12	36.95599725708377	39.0	37.0	39.0	33.0	39.0
13	36.94412923778762	39.0	37.0	39.0	33.0	39.0
14	38.10781144724733	40.0	37.0	41.0	33.0	41.0
15	38.20287288162976	40.0	37.0	41.0	33.0	41.0
16	38.25958494842671	40.0	37.0	41.0	34.0	41.0
17	38.186987423513685	40.0	37.0	41.0	34.0	41.0
18	38.100689678281775	40.0	37.0	41.0	33.0	41.0
19	38.01496636576764	40.0	37.0	41.0	34.0	41.0
20	37.931184157145644	40.0	36.0	41.0	33.0	41.0
21	37.83893504961064	40.0	36.0	41.0	33.0	41.0
22	37.8103594153711	40.0	36.0	41.0	33.0	41.0
23	37.73047616882099	40.0	36.0	41.0	33.0	41.0
24	37.7028244138692	40.0	36.0	41.0	33.0	41.0
25	37.62659240528143	40.0	36.0	41.0	33.0	41.0
26	37.553215805994924	39.0	35.0	41.0	33.0	41.0
27	37.5194355719854	39.0	35.0	41.0	33.0	41.0
28	37.41494267041803	39.0	35.0	41.0	33.0	41.0
29	37.33962855744387	39.0	35.0	41.0	32.0	41.0
30	37.22787985261014	39.0	35.0	41.0	32.0	41.0
31	37.108725752656454	39.0	35.0	41.0	32.0	41.0
32	36.94368764263583	39.0	35.0	41.0	31.0	41.0
33	36.80625341817385	39.0	35.0	41.0	31.0	41.0
34	36.67807833108148	39.0	35.0	41.0	31.0	41.0
35	36.511958372775716	39.0	35.0	41.0	30.0	41.0
36	36.42821326293341	39.0	35.0	41.0	30.0	41.0
37	36.33511208020038	39.0	35.0	41.0	30.0	41.0
38	36.24500153780426	39.0	35.0	41.0	30.0	41.0
39	36.18339123535346	39.0	35.0	41.0	30.0	41.0
40	36.095542521783564	39.0	35.0	41.0	29.0	41.0
41	35.973335551281885	39.0	35.0	41.0	29.0	41.0
42	35.876281553409676	39.0	35.0	40.0	28.0	41.0
43	35.77117233340554	38.0	35.0	40.0	28.0	41.0
44	35.65263215841897	38.0	35.0	40.0	28.0	41.0
45	35.60937976824033	38.0	35.0	40.0	27.0	41.0
46	35.4797674026187	38.0	35.0	40.0	27.0	41.0
47	35.38693548522212	38.0	34.0	40.0	27.0	41.0
48	35.21065883842524	38.0	34.0	40.0	26.0	41.0
49	35.13150799359029	38.0	34.0	40.0	26.0	41.0
50	35.054218071413956	38.0	34.0	40.0	26.0	41.0
51	33.66095664588736	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	19.0
12	10.0
13	17.0
14	23.0
15	44.0
16	91.0
17	180.0
18	369.0
19	648.0
20	1101.0
21	1760.0
22	2606.0
23	3719.0
24	5321.0
25	7146.0
26	8898.0
27	9840.0
28	10630.0
29	12315.0
30	14707.0
31	18074.0
32	23290.0
33	31700.0
34	52472.0
35	66607.0
36	67415.0
37	96784.0
38	163388.0
39	236358.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78138048149429	21.37500044877556	26.028743177115558	12.814875892614591
2	31.97495952044442	25.37257347054297	25.194259981067656	17.458207027944955
3	32.139031865458286	24.722746458562458	28.013168870054944	15.125052805924316
4	27.647805726854852	27.068945090215855	27.427486844892396	17.8557623380369
5	23.667944380552104	31.700189203776418	26.476202329564018	18.155664086107464
6	24.321122250052955	36.54110125932406	25.744758002266614	13.393018488356368
7	81.82423076877049	6.397265700263402	8.146293652398795	3.632209878567317
8	83.37543845372286	5.214412995582852	7.661257026329363	3.748891524364923
9	77.75652908604165	7.758551567902136	9.89520193105132	4.589717415004901
10	37.2591421565401	27.184549674667636	19.721471935969902	15.834836232822367
11	29.237069579359677	27.682750383852696	23.415792352146404	19.664387684641223
12	24.655729308155628	24.99069538670691	30.82585473793302	19.52772056720444
13	24.172368110846367	26.404876933773892	30.823461268275636	18.599293687104105
14	21.783446045808617	29.31784918029648	29.161076917737645	19.73762785615726
15	21.234743126852695	27.86776558836869	32.31219939517022	18.585291889608392
16	23.49214403421704	26.35425505052016	30.997346838884788	19.15625407637801
17	23.67751825918165	25.639924031273075	28.35064809174648	22.331909617798797
18	23.961982127962067	26.003731419195862	29.683810690910917	20.350475761931147
19	23.875936893779013	28.070612141832225	27.98265213192326	20.0707988324655
20	24.769179769915763	27.551827593593643	27.44651492866862	20.232477707821978
21	24.11612157389778	27.92137930869416	28.787097283770958	19.175401833637103
22	22.821015142285788	26.632256551225638	27.56965894254117	22.977069363947404
23	21.710684568224057	27.864773751296962	27.994619480210194	22.429922200268788
24	21.499101850511064	27.138954077694414	29.979523867081053	21.38242020471346
25	22.09842665272072	28.51962704955799	27.876741099583896	21.505205198137403
26	20.960810524564778	28.724627725713166	29.080177643317974	21.234384106404086
27	21.97504329188243	27.750365901673874	28.983122448710937	21.29146835773276
28	21.06588384252406	28.179395337760454	29.93392827010784	20.82079254960765
29	21.898212915880315	27.55864898211719	29.12948311826014	21.413654983742358
30	23.006389367250392	26.62064822338731	29.05456751798393	21.318394891378365
31	23.618519232127063	27.406184964941655	27.115976768983508	21.859319033947777
32	22.19787531698514	27.5082664458292	28.57371946381493	21.720138773370735
33	23.706718589001767	27.508386119312068	26.958007771595977	21.826887520090185
34	22.402516972691707	26.50420592455544	28.371590951248614	22.721686151504237
35	21.669397216634135	26.74307419636264	28.541646970405942	23.045881616597274
36	24.11588222693204	27.53710775520071	27.05937121158631	21.287638806280942
37	22.32975549510715	28.29416220783215	27.516404242664315	21.859678054396383
38	22.508547678513942	28.44088189782996	27.31152323999201	21.73904718366409
39	23.229101718870236	26.756956320375487	27.421144150300318	22.59279781045396
40	22.551031764932556	26.05925991524724	28.12697835226368	23.26272996755652
41	20.535610639930017	26.798483018931147	27.982293111474654	24.683613229664182
42	21.731148733794715	27.202740044063777	27.73432965496938	23.33178156717213
43	22.465943918612457	26.47368918642376	28.941715423638147	22.118651471325634
44	22.0020894990109	26.53424396875565	28.68872568085236	22.774940851381093
45	22.460079917951862	26.04238595416266	28.733483563445496	22.764050564439984
46	22.796601751780443	26.71219843778235	28.02609360620483	22.46510620423237
47	22.126190900746405	25.941142187655203	29.039368985659525	22.893297925938867
48	21.582633941553865	26.797405957585323	28.65282363599156	22.96713646486925
49	22.329157127692802	26.121250779373558	29.15976050942608	22.389831583507558
50	21.31911293227558	26.19832050234141	28.975343672324428	23.50722289305858
51	21.76106710451205	25.58463488218744	27.582344331725324	25.071953681575188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3301.0
1	3144.5
2	2988.0
3	4432.5
4	5877.0
5	4391.5
6	2906.0
7	2987.0
8	3068.0
9	3223.5
10	3379.0
11	3502.0
12	3625.0
13	3581.5
14	3538.0
15	3488.5
16	3439.0
17	3241.5
18	3044.0
19	3309.0
20	3574.0
21	3542.0
22	3510.0
23	4168.5
24	4827.0
25	5409.0
26	7269.0
27	8547.0
28	10249.5
29	11952.0
30	13150.0
31	14348.0
32	16230.0
33	18112.0
34	20367.0
35	22622.0
36	25905.0
37	29188.0
38	30827.5
39	32467.0
40	35028.5
41	37590.0
42	42405.0
43	47220.0
44	51711.5
45	56203.0
46	61857.5
47	67512.0
48	71485.0
49	75458.0
50	74064.0
51	72670.0
52	64905.0
53	57140.0
54	52649.5
55	48159.0
56	44159.0
57	40159.0
58	36489.5
59	32820.0
60	30134.5
61	27449.0
62	24579.5
63	21710.0
64	19204.5
65	16699.0
66	14162.0
67	11625.0
68	10540.0
69	9455.0
70	8539.0
71	7623.0
72	6764.0
73	5905.0
74	4734.5
75	3162.0
76	2760.0
77	2289.5
78	1819.0
79	1347.0
80	875.0
81	619.5
82	364.0
83	268.5
84	173.0
85	177.5
86	182.0
87	119.5
88	57.0
89	39.5
90	22.0
91	23.0
92	24.0
93	31.0
94	38.0
95	32.0
96	26.0
97	14.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	835607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.973328660827157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91206189094328	20.955397279355818
2	9.263459613454996	5.18259600606951
3	3.469792617274161	2.9118494780376527
4	1.828279369247841	2.0457223871911854
5	1.1655656034714572	1.630237485083121
6	0.8795890916226722	1.4763020847863653
7	0.6752154786864261	1.3221617151521192
8	0.5447158680828648	1.2190012803719796
9	0.4712948695536604	1.1865317653967584
>10	5.585106173169432	36.090381169660276
>50	1.1101795126642977	20.57523728768233
>100	0.09000291623746381	3.7727873749053216
>500	0.0021531798142933926	0.3966853611406247
>1k	0.0025838157771520716	1.2351093251669676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2879	0.3445399571808278	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2780	0.33269228237676324	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1202	0.14384752640894582	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	1137	0.13606875002243876	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	1134	0.13570972957383076	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	1121	0.13415397429652937	No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	886	0.10603070582223462	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.196734828693393E-4	0.0	0.0	0.10974058379118413	0.0
2	1.196734828693393E-4	0.0	0.0	0.5132795680265962	0.0
3	1.196734828693393E-4	0.0	0.0	0.7126555904869155	0.0
4	1.196734828693393E-4	0.0	0.0	0.970671619553211	0.0
5	1.196734828693393E-4	0.0	0.0	1.7540542384159061	0.0
6	1.196734828693393E-4	0.0	0.0	2.3551741428685973	0.0
7	1.196734828693393E-4	0.0	0.0	2.9023213065472167	0.0
8	1.196734828693393E-4	0.0	0.0	3.7640900566893287	0.0
9	1.196734828693393E-4	0.0	0.0	4.15518299870633	0.0
10	1.196734828693393E-4	0.0	0.0	4.986554684199629	0.0
11	1.196734828693393E-4	0.0	0.0	6.0558372536371765	0.0
12	1.196734828693393E-4	0.0	0.0	6.824380360624073	0.0
13	1.196734828693393E-4	0.0	0.0	7.14558398864538	0.0
14	1.196734828693393E-4	0.0	0.0	7.275788738007221	0.0
15	1.196734828693393E-4	0.0	0.0	7.4782762710221435	0.0
16	1.196734828693393E-4	0.0	0.0	7.941292976243617	0.0
17	1.196734828693393E-4	0.0	0.0	8.492628711822663	0.0
18	1.196734828693393E-4	0.0	0.0	9.143891805597608	0.0
19	1.196734828693393E-4	0.0	0.0	9.496449886130682	0.0
20	1.196734828693393E-4	0.0	0.0	9.822919147398238	0.0
21	1.196734828693393E-4	0.0	0.0	10.220833477938791	0.0
22	1.196734828693393E-4	0.0	0.0	10.658958098723444	0.0
23	1.196734828693393E-4	0.0	0.0	11.140045499858187	0.0
24	1.196734828693393E-4	0.0	0.0	11.525513788180328	0.0
25	1.196734828693393E-4	0.0	0.0	11.855573253933967	0.0
26	1.196734828693393E-4	0.0	0.0	12.158466839076265	0.0
27	1.196734828693393E-4	0.0	0.0	12.477276997440184	0.0
28	1.196734828693393E-4	0.0	0.0	12.812961116888681	0.0
29	1.196734828693393E-4	0.0	0.0	13.149482950717262	0.0
30	1.196734828693393E-4	0.0	0.0	13.540695566217133	0.0
31	2.393469657386786E-4	0.0	0.0	13.882483033291965	0.0
32	2.393469657386786E-4	0.0	0.0	14.238032950896773	0.0
33	2.393469657386786E-4	0.0	0.0	14.582094214146125	0.0
34	2.393469657386786E-4	0.0	0.0	14.913350414728455	0.0
35	2.393469657386786E-4	0.0	0.0	15.25657396359772	0.0
36	2.393469657386786E-4	0.0	0.0	15.589505592940222	0.0
37	3.590204486080179E-4	0.0	0.0	15.929378284289145	0.0
38	3.590204486080179E-4	0.0	0.0	16.2691313021552	0.0
39	4.786939314773572E-4	0.0	0.0	16.587223419621903	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.03218E-4	45.0	1
ACCGGAT	20	7.03218E-4	45.0	35
CACGGAT	20	7.03218E-4	45.0	4
ATAATCG	20	7.03218E-4	45.0	38
TATATCG	20	7.03218E-4	45.0	33
TTCGTGC	20	7.03218E-4	45.0	36
CGACATA	20	7.03218E-4	45.0	32
TGACGGA	20	7.03218E-4	45.0	29
ATATCGC	20	7.03218E-4	45.0	34
AACGCCG	20	7.03218E-4	45.0	13
CGTACTC	25	3.8898892E-5	45.0	45
ACGTCGC	20	7.03218E-4	45.0	20
CGCGTAC	20	7.03218E-4	45.0	43
ATACCGG	20	7.03218E-4	45.0	2
GCCCGTA	20	7.03218E-4	45.0	9
GTCGGTT	20	7.03218E-4	45.0	12
CAATGCG	30	2.1647884E-6	44.999996	1
GTACGAT	30	2.1647884E-6	44.999996	10
CCCGCTA	80	0.0	42.1875	23
GTTACGG	70	0.0	41.785717	2
>>END_MODULE