SRR3553906_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498490
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1678	0.3366165820778752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.28004573812915	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	743	0.14905013139681839	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	731	0.14664286144155347	Illumina PCR Primer Index 6 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	607	0.12176773857048287	RNA PCR Primer, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	575	0.11534835202310978	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	558	0.11193805291981784	RNA PCR Primer, Index 27 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2100827E-7	45.0	1
ACGCATC	20	7.0294394E-4	45.0	38
TTTCGAC	20	7.0294394E-4	45.0	24
ACTTACG	20	7.0294394E-4	45.0	1
CCCGTCA	20	7.0294394E-4	45.0	23
CGCTACA	35	1.2100827E-7	45.0	28
CTACCGT	25	3.8876162E-5	45.0	2
TTGCCCG	20	7.0294394E-4	45.0	1
ACCCGAT	20	7.0294394E-4	45.0	40
TTTGACG	25	3.8876162E-5	45.0	1
CGGTATT	20	7.0294394E-4	45.0	29
TCGACGT	20	7.0294394E-4	45.0	26
TAATGCG	35	1.2100827E-7	45.0	1
CTAGCGG	30	2.1630185E-6	44.999996	2
CGCAAGG	70	0.0	41.785713	2
TTTCGCG	50	1.0786607E-9	40.5	1
CGTTTTT	1155	0.0	39.545456	1
CGGGTGA	105	0.0	38.57143	6
AATCGAG	35	6.241171E-6	38.571426	1
TTCGCGG	100	0.0	38.25	2