##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498490
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.968681417882003	33.0	31.0	34.0	30.0	34.0
2	32.113635178238276	33.0	31.0	34.0	30.0	34.0
3	32.13824550141427	34.0	31.0	34.0	30.0	34.0
4	35.69777327529138	37.0	35.0	37.0	33.0	37.0
5	35.62685309635098	37.0	35.0	37.0	33.0	37.0
6	35.602668057533755	37.0	35.0	37.0	33.0	37.0
7	35.83958153623944	37.0	35.0	37.0	35.0	37.0
8	35.77492226524103	37.0	35.0	37.0	35.0	37.0
9	37.556195711047366	39.0	37.0	39.0	35.0	39.0
10	36.81802242773175	39.0	37.0	39.0	32.0	39.0
11	36.806419386547375	39.0	37.0	39.0	32.0	39.0
12	36.79147425224177	39.0	35.0	39.0	33.0	39.0
13	36.812088507292025	39.0	37.0	39.0	33.0	39.0
14	37.9418403578808	40.0	37.0	41.0	33.0	41.0
15	38.034923468876	40.0	37.0	41.0	33.0	41.0
16	38.087480190174325	40.0	37.0	41.0	33.0	41.0
17	38.04934301590804	40.0	37.0	41.0	33.0	41.0
18	37.98342394029971	40.0	37.0	41.0	33.0	41.0
19	37.88313306184678	40.0	37.0	41.0	33.0	41.0
20	37.814935104014125	40.0	36.0	41.0	33.0	41.0
21	37.7182531244358	39.0	36.0	41.0	33.0	41.0
22	37.70886276555197	39.0	36.0	41.0	33.0	41.0
23	37.652771369536	39.0	36.0	41.0	33.0	41.0
24	37.621577163032356	39.0	36.0	41.0	33.0	41.0
25	37.580246343958756	39.0	36.0	41.0	33.0	41.0
26	37.518754639009806	39.0	36.0	41.0	33.0	41.0
27	37.48427049690064	39.0	36.0	41.0	33.0	41.0
28	37.408938995767215	39.0	36.0	41.0	33.0	41.0
29	37.33119019438705	39.0	36.0	41.0	32.0	41.0
30	37.217853918834884	39.0	35.0	41.0	32.0	41.0
31	37.091343858452525	39.0	35.0	41.0	31.0	41.0
32	36.985931513169774	39.0	35.0	41.0	31.0	41.0
33	36.85808742402054	39.0	35.0	41.0	31.0	41.0
34	36.74050632911393	39.0	35.0	41.0	31.0	41.0
35	36.619982346686996	39.0	35.0	41.0	30.0	41.0
36	36.540510341230515	39.0	35.0	41.0	30.0	41.0
37	36.46733535276535	39.0	35.0	41.0	30.0	41.0
38	36.42995045036009	39.0	35.0	41.0	30.0	41.0
39	36.349559670204016	39.0	35.0	41.0	30.0	41.0
40	36.27647896647876	39.0	35.0	41.0	30.0	41.0
41	36.145391081064815	39.0	35.0	41.0	29.0	41.0
42	36.04969407610985	39.0	35.0	40.0	29.0	41.0
43	35.99793777207166	39.0	35.0	40.0	29.0	41.0
44	35.91173744709021	39.0	35.0	40.0	28.0	41.0
45	35.85740737025818	39.0	35.0	40.0	28.0	41.0
46	35.73010692290718	38.0	35.0	40.0	28.0	41.0
47	35.63551124395675	38.0	35.0	40.0	28.0	41.0
48	35.49671608256936	38.0	34.0	40.0	27.0	41.0
49	35.45197095227587	38.0	34.0	40.0	27.0	41.0
50	35.38182511183775	38.0	34.0	40.0	27.0	41.0
51	34.00596401131417	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	2.0
11	6.0
12	7.0
13	4.0
14	13.0
15	20.0
16	56.0
17	91.0
18	195.0
19	352.0
20	582.0
21	925.0
22	1390.0
23	2128.0
24	3042.0
25	4104.0
26	5015.0
27	5974.0
28	6377.0
29	7189.0
30	8852.0
31	11208.0
32	14382.0
33	19436.0
34	30669.0
35	39073.0
36	40631.0
37	59581.0
38	100272.0
39	136883.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81510160685269	20.297297839475213	25.147144376015568	11.740456177656524
2	33.47489418042488	24.984854259864793	24.438002768360448	17.10224879134988
3	31.495315853878715	24.672310377339564	27.547794338903486	16.28457942987823
4	26.40975746755201	27.111476659511723	27.922726634435996	18.556039238500272
5	23.08531765933118	31.828722742682903	26.605147545587677	18.480812052398242
6	23.401873658448512	37.59012216895023	26.14435595498405	12.863648217617204
7	82.35330698710105	6.080763906999138	7.999157455515657	3.5667716503841604
8	83.67991333828161	4.9916748580713755	7.486208349214627	3.8422034544323855
9	77.75421773756746	8.098457341170334	9.322152901763324	4.825172019498886
10	35.44624766795723	26.84968605187667	21.610664205901823	16.09340207426428
11	28.385925494994886	28.788742000842543	23.55593893558547	19.269393568577105
12	23.462055407330137	25.379646532528234	31.469638307689223	19.688659752452406
13	23.57379285442035	27.077574274308414	31.60564906016169	17.74298381110955
14	21.484683744909628	29.766494814339307	30.08024233184216	18.668579108908904
15	21.055587875383658	28.440490280647555	32.35571425705631	18.148207586912477
16	22.530642540472225	26.555397299845534	30.99961884892375	19.91434131075849
17	22.819514935104014	27.080382755922887	28.584324660474635	21.515777648498467
18	23.464262071455796	26.86693815322273	29.58193745110233	20.086862324219144
19	23.158137575477944	29.11312162731449	28.14138698870589	19.587353808501675
20	22.990431101927822	28.25412746494413	28.368472787819215	20.386968645308833
21	22.554514634195268	28.191939657766458	29.655760396397117	19.597785311641154
22	21.662019298280807	26.580272422716604	28.648719131777973	23.10898914722462
23	20.372725631406848	28.70328391743064	28.78152019097675	22.14247026018576
24	20.357278982527234	27.726935344741115	30.361090493289733	21.554695179441914
25	20.413248008987143	29.10429497081185	28.641497321912173	21.84095969828883
26	20.39017833858252	29.095668920138817	28.583121025497	21.93103171578166
27	21.22249192561536	27.798150414251037	28.732772974382637	22.246584685750967
28	20.465204918854944	28.154426367630244	29.029268390539432	22.351100322975388
29	22.032137053902787	26.829224257256918	28.30147044073101	22.83716824810929
30	21.91698930770928	27.383096952797448	28.123332464041408	22.576581275451865
31	21.129611426508056	28.30969527974483	26.872555116451686	23.688138177295432
32	21.08126542157315	27.344179421853998	27.695039017833857	23.87951613873899
33	21.205641035928505	26.857509679231278	27.97668960259985	23.960159682240363
34	20.558085417962246	27.539770105719274	28.789143212501756	23.113001263816727
35	21.046961824710625	27.004353146502435	28.059941021886097	23.888744006900843
36	21.887700856586893	27.063130654576824	28.25813958153624	22.791028907300046
37	20.037312684306606	28.884631587393926	27.838070974342514	23.23998475395695
38	20.236915484763987	28.11651186583482	28.709903909807622	22.93666873959357
39	21.18939196373047	27.178880218259145	28.870388573491944	22.761339244518446
40	21.352083291540453	26.75600312945094	29.73158939998796	22.160324179020645
41	19.88906497622821	27.67838873397661	29.198579710726392	23.233966579068788
42	20.421472848000963	26.624806916889003	29.448133362755524	23.505586872354513
43	20.544845433208287	26.094806315071516	29.264980240325784	24.09536801139441
44	20.616662320207023	26.425204116431622	28.029047724126865	24.92908583923449
45	21.140042929647535	26.96704046219583	27.737567453710206	24.15534915444643
46	21.917189913538888	26.803546711067423	28.398563662260024	22.880699713133666
47	20.386968645308833	27.276775863106582	29.69247126321491	22.643784228369675
48	20.063592047984915	26.104435394892576	29.65696403137475	24.175008525747756
49	20.769323356536738	25.62578988545407	29.951252783405884	23.653633974603302
50	20.755682160123573	24.787056911873858	29.579530181147064	24.8777307468555
51	19.80822082689723	25.995506429416835	27.887219402595843	26.309053341090095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1874.0
1	1694.0
2	1514.0
3	2249.5
4	2985.0
5	2214.5
6	1444.0
7	1480.0
8	1516.0
9	1622.0
10	1728.0
11	1769.5
12	1811.0
13	1796.0
14	1781.0
15	1811.5
16	1842.0
17	1781.0
18	1720.0
19	1610.5
20	1501.0
21	1660.0
22	1819.0
23	2159.5
24	2500.0
25	2921.0
26	3677.0
27	4012.0
28	5573.5
29	7135.0
30	7770.0
31	8405.0
32	10117.5
33	11830.0
34	12933.5
35	14037.0
36	16404.5
37	18772.0
38	19210.5
39	19649.0
40	21361.5
41	23074.0
42	27275.0
43	31476.0
44	34082.0
45	36688.0
46	39619.5
47	42551.0
48	45962.0
49	49373.0
50	48649.5
51	47926.0
52	43094.0
53	38262.0
54	34078.5
55	29895.0
56	26785.5
57	23676.0
58	20775.5
59	17875.0
60	15999.5
61	14124.0
62	12231.0
63	10338.0
64	8961.0
65	7584.0
66	6306.5
67	5029.0
68	3920.0
69	2811.0
70	2417.0
71	2023.0
72	1844.5
73	1666.0
74	1421.5
75	893.0
76	609.0
77	491.5
78	374.0
79	351.0
80	328.0
81	272.0
82	216.0
83	157.0
84	98.0
85	66.5
86	35.0
87	26.5
88	18.0
89	18.0
90	18.0
91	16.0
92	14.0
93	10.5
94	7.0
95	5.5
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.09834677737305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88664455216981	26.28397419485931
2	9.361410984619264	6.571400981273524
3	3.3976315491671705	3.5775375100323776
4	1.9614161980888876	2.7536986358112165
5	1.3335397736788792	2.340252070900044
6	1.0051551787435629	2.116757101716838
7	0.7674159155264995	1.8854520947937017
8	0.7119963360080293	1.999191544434307
9	0.6103556732497694	1.9280227569531658
>10	5.67424337336508	41.93953379272896
>50	0.25686165945786454	5.522398299734939
>100	0.029306363830763044	1.8135716559372022
>500	0.0028731729245846122	0.648222787164387
>1k	0.001149269169833845	0.6199865736600266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1678	0.3366165820778752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.28004573812915	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	743	0.14905013139681839	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	731	0.14664286144155347	Illumina PCR Primer Index 6 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	607	0.12176773857048287	RNA PCR Primer, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	575	0.11534835202310978	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	558	0.11193805291981784	RNA PCR Primer, Index 27 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11635138117113683	0.0
2	0.0	0.0	0.0	0.5247848502477482	0.0
3	0.0	0.0	0.0	0.7562839776123894	0.0
4	0.0	0.0	0.0	1.0926999538606592	0.0
5	0.0	0.0	0.0	2.085096992918614	0.0
6	0.0	0.0	0.0	2.860639130173123	0.0
7	0.0	0.0	0.0	3.5019759674216133	0.0
8	0.0	0.0	0.0	4.536901442355915	0.0
9	0.0	0.0	0.0	4.992076069730587	0.0
10	0.0	0.0	0.0	5.97664948143393	0.0
11	0.0	0.0	0.0	7.190916568035467	0.0
12	0.0	0.0	0.0	8.10387369856968	0.0
13	0.0	0.0	0.0	8.49886657706273	0.0
14	0.0	0.0	0.0	8.672992437160223	0.0
15	2.0060582960540834E-4	0.0	0.0	8.931573351521596	0.0
16	2.0060582960540834E-4	0.0	0.0	9.554855664105599	0.0
17	2.0060582960540834E-4	0.0	0.0	10.275833015707436	0.0
18	2.0060582960540834E-4	0.0	0.0	11.086681778972498	0.0
19	4.012116592108167E-4	0.0	0.0	11.509358261951093	0.0
20	4.012116592108167E-4	0.0	0.0	11.92621717587113	0.0
21	4.012116592108167E-4	0.0	0.0	12.415494794278722	0.0
22	6.01817488816225E-4	0.0	0.0	13.040181347669963	0.0
23	6.01817488816225E-4	0.0	0.0	13.643804288952637	0.0
24	6.01817488816225E-4	0.0	0.0	14.116632229332584	0.0
25	6.01817488816225E-4	0.0	0.0	14.556962025316455	0.0
26	6.01817488816225E-4	0.0	0.0	14.968203976007542	0.0
27	8.024233184216334E-4	0.0	0.0	15.37021805853678	0.0
28	8.024233184216334E-4	0.0	0.0	15.783466067523923	0.0
29	8.024233184216334E-4	0.0	0.0	16.213564966197918	0.0
30	8.024233184216334E-4	0.0	0.0	16.682982607474575	0.0
31	8.024233184216334E-4	0.0	0.0	17.159421452787416	0.0
32	8.024233184216334E-4	0.0	0.0	17.61379365684367	0.0
33	8.024233184216334E-4	0.0	0.0	18.055728299464384	0.0
34	8.024233184216334E-4	0.0	0.0	18.469778731769946	0.0
35	8.024233184216334E-4	0.0	0.0	18.88804188649722	0.0
36	8.024233184216334E-4	0.0	0.0	19.317338361852794	0.0
37	0.0010030291480270417	0.0	0.0	19.759473610303115	0.0
38	0.0010030291480270417	0.0	0.0	20.193785231398824	0.0
39	0.0010030291480270417	0.0	0.0	20.65317258119521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2100827E-7	45.0	1
ACGCATC	20	7.0294394E-4	45.0	38
TTTCGAC	20	7.0294394E-4	45.0	24
ACTTACG	20	7.0294394E-4	45.0	1
CCCGTCA	20	7.0294394E-4	45.0	23
CGCTACA	35	1.2100827E-7	45.0	28
CTACCGT	25	3.8876162E-5	45.0	2
TTGCCCG	20	7.0294394E-4	45.0	1
ACCCGAT	20	7.0294394E-4	45.0	40
TTTGACG	25	3.8876162E-5	45.0	1
CGGTATT	20	7.0294394E-4	45.0	29
TCGACGT	20	7.0294394E-4	45.0	26
TAATGCG	35	1.2100827E-7	45.0	1
CTAGCGG	30	2.1630185E-6	44.999996	2
CGCAAGG	70	0.0	41.785713	2
TTTCGCG	50	1.0786607E-9	40.5	1
CGTTTTT	1155	0.0	39.545456	1
CGGGTGA	105	0.0	38.57143	6
AATCGAG	35	6.241171E-6	38.571426	1
TTCGCGG	100	0.0	38.25	2
>>END_MODULE