##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317923
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08014519238935	33.0	31.0	34.0	30.0	34.0
2	32.230065141559436	34.0	31.0	34.0	30.0	34.0
3	32.25370923148058	34.0	31.0	34.0	30.0	34.0
4	35.79725908474693	37.0	35.0	37.0	35.0	37.0
5	35.710495937695605	37.0	35.0	37.0	33.0	37.0
6	35.690088480544034	37.0	35.0	37.0	33.0	37.0
7	35.80841901969974	37.0	35.0	37.0	35.0	37.0
8	35.700314227029814	37.0	35.0	37.0	35.0	37.0
9	37.54457840420479	39.0	37.0	39.0	35.0	39.0
10	37.04321801190854	39.0	37.0	39.0	33.0	39.0
11	36.92122935427761	39.0	37.0	39.0	33.0	39.0
12	36.76450587091843	39.0	35.0	39.0	33.0	39.0
13	36.64081868880201	39.0	35.0	39.0	33.0	39.0
14	37.74485016812247	40.0	36.0	41.0	33.0	41.0
15	37.87091213910286	40.0	36.0	41.0	33.0	41.0
16	37.96641954183875	40.0	36.0	41.0	33.0	41.0
17	37.920115877114895	40.0	36.0	41.0	33.0	41.0
18	37.836243367104615	39.0	36.0	41.0	33.0	41.0
19	37.75501929712541	39.0	36.0	41.0	33.0	41.0
20	37.68465005677476	39.0	35.0	41.0	33.0	41.0
21	37.57953969986443	39.0	35.0	41.0	33.0	41.0
22	37.52174897695354	39.0	35.0	41.0	33.0	41.0
23	37.43156047218981	39.0	35.0	41.0	33.0	41.0
24	37.402427631847964	39.0	35.0	41.0	33.0	41.0
25	37.30964730453601	39.0	35.0	41.0	33.0	41.0
26	37.21074599824486	39.0	35.0	41.0	32.0	41.0
27	37.17246314359137	39.0	35.0	41.0	32.0	41.0
28	37.04598283232103	39.0	35.0	41.0	32.0	41.0
29	36.992211950692464	39.0	35.0	41.0	32.0	41.0
30	36.81117440386509	39.0	35.0	41.0	31.0	41.0
31	36.687424313434384	39.0	35.0	41.0	31.0	41.0
32	36.57404780402802	39.0	35.0	41.0	31.0	41.0
33	36.47855927378642	39.0	35.0	41.0	31.0	41.0
34	36.28131654520119	39.0	35.0	41.0	30.0	41.0
35	36.16175614850137	38.0	35.0	41.0	30.0	41.0
36	35.99457415789358	38.0	35.0	40.0	30.0	41.0
37	35.92420491754293	38.0	35.0	40.0	30.0	41.0
38	35.81397696926614	38.0	35.0	40.0	29.0	41.0
39	35.76705994847809	38.0	35.0	40.0	29.0	41.0
40	35.617942080315046	38.0	35.0	40.0	28.0	41.0
41	35.48522440968411	38.0	34.0	40.0	28.0	41.0
42	35.38212711883066	38.0	34.0	40.0	27.0	41.0
43	35.24520402739028	38.0	34.0	40.0	27.0	41.0
44	35.081069944609226	37.0	34.0	40.0	27.0	41.0
45	34.96228961100644	37.0	34.0	40.0	26.0	41.0
46	34.80959225976101	37.0	34.0	40.0	26.0	41.0
47	34.71868030938309	37.0	34.0	40.0	26.0	41.0
48	34.55978334376563	36.0	34.0	40.0	25.0	41.0
49	34.52714021948711	36.0	34.0	40.0	25.0	41.0
50	34.398939365821285	36.0	33.0	40.0	24.0	41.0
51	32.98635204121753	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	2.0
12	3.0
13	6.0
14	14.0
15	20.0
16	54.0
17	91.0
18	163.0
19	294.0
20	459.0
21	727.0
22	1089.0
23	1573.0
24	2048.0
25	2677.0
26	3237.0
27	3724.0
28	4429.0
29	5272.0
30	6281.0
31	7986.0
32	10247.0
33	14091.0
34	24638.0
35	31552.0
36	27639.0
37	38228.0
38	58416.0
39	72936.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.581621965067015	20.095117371187364	24.353066623050236	11.970194040695388
2	32.06845682759662	25.481956322757398	25.042227205958678	17.407359643687307
3	31.692579649789415	25.40017551419683	26.80523271358159	16.10201212243216
4	27.831581861016662	27.807676701591266	25.163011169371202	19.197730268020873
5	23.92151558710757	31.513605495670333	24.414402229470657	20.150476687751436
6	23.918999254536477	37.50310609801745	23.94982432853238	14.6280703189137
7	80.69784193027871	8.154490238202332	7.253957719321974	3.8937101121969784
8	82.08685750952274	6.690299223396861	6.690928306539634	4.53191496054076
9	77.09948635361393	8.822576535827857	8.594219355001053	5.483717755557163
10	41.13039949924982	26.397272295492936	16.060492635009105	16.411835570248144
11	31.863690264623823	27.44658297763924	20.742129383529974	19.947597374206964
12	27.908959087577813	23.146799696781926	28.487086495786716	20.45715471985355
13	25.129984304375586	26.519943508333775	29.146994712556186	19.20307747473445
14	20.661921282826345	29.78299776990026	28.14140530883264	21.413675638440754
15	19.69344778452644	27.4475266023534	31.436228269109183	21.42279734401097
16	21.640145569839238	24.910434287547613	32.06877136916801	21.380648773445145
17	21.589818918417354	24.25304240334924	27.819314739732576	26.337823938500833
18	22.787278680686832	25.30895845849467	28.575158135775013	23.328604725043487
19	24.57733476344901	27.102159956970713	25.665648600447277	22.654856679132997
20	25.45742208018923	26.46112423448445	25.773536359432946	22.307917325893374
21	24.701578684146792	27.096183667114364	26.64198563803185	21.560252010706996
22	23.369495129323766	26.08776338924834	25.00133680167839	25.541404679749498
23	22.00155383536265	28.98123130443535	24.579851096020107	24.437363764181892
24	22.495698644011284	27.029500853980366	27.51704028962988	22.95776021237847
25	22.639444142135044	27.510120375059373	25.80247418400053	24.047961298805056
26	21.03087854606304	28.60220871091428	26.311402446504346	24.05551029651834
27	22.53218546629215	27.307555603086282	26.700175828738402	23.46008310188316
28	20.44866209742611	27.795095038735795	30.314573025543922	21.441669838294178
29	23.32514476775823	26.311402446504346	27.622411716044454	22.741041069692976
30	23.581496148438458	27.067874925689555	26.979174202558482	22.37145472331351
31	24.895650833692436	27.331775304083067	24.82865347898705	22.94392038323745
32	24.09262620194198	27.929718831289335	25.213337820793086	22.7643171459756
33	26.500756472479186	25.328774577492037	24.66635002815147	23.50411892187731
34	23.29777965104758	26.25478496365472	26.877577275000554	23.569858110297147
35	23.50978067016227	25.598022162599122	25.78674710543119	25.105450061807417
36	26.46395510862693	26.872544609858362	26.016991535686312	20.646508745828392
37	22.96247833594927	27.031388103408688	28.412540143368048	21.593593417273993
38	23.359744340610778	27.741622971600044	26.00881345483026	22.889819232958924
39	24.54996964673836	24.646533909154105	28.017790471277635	22.7857059728299
40	25.37406856377173	24.140751062364156	28.53143685735225	21.95374351651186
41	20.648081453685325	24.795626613991438	28.098313113552653	26.457978818770584
42	22.88227023524564	24.844380557556388	27.430541357498512	24.842807849699454
43	23.339613680042024	23.36729333832406	29.128122218272978	24.16497076336094
44	22.944864007951608	24.7846176589929	28.27414185195786	23.996376481097627
45	24.428242058611676	25.359285109916552	27.021637314695695	23.190835516776076
46	24.428556600183065	25.805619599714397	27.32800080522642	22.437822994876118
47	23.026644816512174	25.72132245858274	29.54048621836105	21.71154650654404
48	22.77029343583195	25.7864325638598	27.634678837328536	23.808595162979714
49	24.008958143953095	24.549340563595585	29.011427295288485	22.430273997162836
50	22.329935235890453	24.948493817685414	29.08691727242131	23.634653674002827
51	23.17479389663535	24.22693545292414	25.683892011587712	26.914378638852803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2052.0
1	2057.5
2	2063.0
3	1893.0
4	1723.0
5	1318.0
6	913.0
7	945.5
8	978.0
9	1016.0
10	1054.0
11	1108.0
12	1162.0
13	1182.0
14	1202.0
15	1219.0
16	1236.0
17	1220.5
18	1205.0
19	1196.0
20	1187.0
21	1280.5
22	1374.0
23	1310.5
24	1247.0
25	1622.5
26	2585.0
27	3172.0
28	3228.5
29	3285.0
30	3850.0
31	4415.0
32	4781.5
33	5148.0
34	5766.0
35	6384.0
36	6424.5
37	6465.0
38	7690.0
39	8915.0
40	9722.5
41	10530.0
42	12480.5
43	14431.0
44	16625.0
45	18819.0
46	21342.5
47	23866.0
48	25802.5
49	27739.0
50	28266.5
51	28794.0
52	26706.0
53	24618.0
54	22197.5
55	19777.0
56	18644.0
57	17511.0
58	16266.0
59	15021.0
60	14480.5
61	13940.0
62	12873.5
63	11807.0
64	10181.0
65	8555.0
66	7897.5
67	7240.0
68	6608.0
69	5976.0
70	4933.0
71	3890.0
72	3342.5
73	2795.0
74	2647.5
75	1858.0
76	1216.0
77	1075.0
78	934.0
79	667.5
80	401.0
81	293.5
82	186.0
83	129.5
84	73.0
85	70.5
86	68.0
87	47.0
88	26.0
89	20.0
90	14.0
91	11.5
92	9.0
93	8.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	317923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.658197110621124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.21570392159015	22.12862863020291
2	8.551107989156085	4.901186765348842
3	2.8492717674045944	2.4496497579602607
4	1.4334163822151003	1.643165168924551
5	1.0536598215363677	1.5097995426565551
6	0.704635005652446	1.2116141329818855
7	0.5586592178770949	1.1207116188511055
8	0.5037811021720758	1.1549966501322646
9	0.4313419894414506	1.1125335379950492
>10	5.626104422078564	39.58600038374072
>50	1.0042695174018506	18.278010713285923
>100	0.06146348958962145	3.0925727298748438
>500	0.004390249256401532	0.8807163998829906
>1k	0.002195124628200766	0.9304139681621021
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1844	0.5800146576372266	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1114	0.35039931052487555	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	951	0.29912903438883	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	773	0.24314063468198274	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.17362694740550383	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	524	0.16481978340667394	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	483	0.15192357897981587	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	482	0.15160903740842907	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	454	0.14280187340959918	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	436	0.13714012512463708	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	420	0.13210745998244858	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	341	0.10725867584289277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17362694740550386	0.0
2	0.0	0.0	0.0	0.8917253548815279	0.0
3	0.0	0.0	0.0	1.08799929542688	0.0
4	0.0	0.0	0.0	1.333656262679957	0.0
5	0.0	0.0	0.0	2.1753695077109865	0.0
6	0.0	0.0	0.0	2.677692397215678	0.0
7	0.0	0.0	0.0	3.1655463744365773	0.0
8	0.0	0.0	0.0	3.975490920757542	0.0
9	0.0	0.0	0.0	4.386596754560067	0.0
10	0.0	0.0	0.0	5.471136092701692	0.0
11	0.0	0.0	0.0	6.985339217357662	0.0
12	0.0	0.0	0.0	8.025213652362364	0.0
13	0.0	0.0	0.0	8.397001789741541	0.0
14	3.1454157138678234E-4	0.0	0.0	8.526592917152895	0.0
15	3.1454157138678234E-4	0.0	0.0	8.762813637264369	0.0
16	3.1454157138678234E-4	0.0	0.0	9.312946845619852	0.0
17	3.1454157138678234E-4	0.0	0.0	9.911833997540285	0.0
18	3.1454157138678234E-4	0.0	0.0	10.608543578162008	0.0
19	6.290831427735647E-4	0.0	0.0	10.944788517974478	0.0
20	6.290831427735647E-4	0.0	0.0	11.265620920788995	0.0
21	6.290831427735647E-4	0.0	0.0	11.660685134450794	0.0
22	6.290831427735647E-4	0.0	0.0	12.029013314544716	0.0
23	6.290831427735647E-4	0.0	0.0	12.424706611349288	0.0
24	6.290831427735647E-4	0.0	0.0	12.764411508447013	0.0
25	9.43624714160347E-4	0.0	0.0	13.013528432985346	0.0
26	9.43624714160347E-4	0.0	0.0	13.293155889948196	0.0
27	9.43624714160347E-4	0.0	0.0	13.597946672621987	0.0
28	9.43624714160347E-4	0.0	0.0	13.897075707010817	0.0
29	9.43624714160347E-4	0.0	0.0	14.188970285257751	0.0
30	9.43624714160347E-4	0.0	0.0	14.516093519500005	0.0
31	9.43624714160347E-4	0.0	0.0	14.788801061892345	0.0
32	9.43624714160347E-4	0.0	0.0	15.06874306042658	0.0
33	9.43624714160347E-4	0.0	0.0	15.354975890388554	0.0
34	9.43624714160347E-4	0.0	0.0	15.622336226067318	0.0
35	9.43624714160347E-4	0.0	0.0	15.940337754739355	0.0
36	0.0012581662855471294	0.0	0.0	16.212730755560308	0.0
37	0.0012581662855471294	0.0	0.0	16.506198041664177	0.0
38	0.0012581662855471294	0.0	0.0	16.7745020020571	0.0
39	0.0012581662855471294	0.0	0.0	17.062936623018782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	45	3.8380676E-10	45.000004	17
TTCAACG	25	3.8844177E-5	45.000004	1
TCTAGTC	25	3.8844177E-5	45.000004	29
GCCGATG	50	2.1827873E-11	45.000004	9
TACGCAT	45	3.8380676E-10	45.000004	16
TTCGCGT	25	3.8844177E-5	45.000004	40
CTCTAGT	25	3.8844177E-5	45.000004	28
TAGTCGG	25	3.8844177E-5	45.000004	31
TACCCGA	20	7.02558E-4	45.0	37
CGTATTA	20	7.02558E-4	45.0	17
CTCGTCT	20	7.02558E-4	45.0	35
GATCGCT	20	7.02558E-4	45.0	10
CAACGGT	20	7.02558E-4	45.0	44
CGTGCGG	35	1.2082273E-7	45.0	2
CGCATCG	20	7.02558E-4	45.0	21
GGCCGAT	75	0.0	45.0	8
GGATCGC	40	6.7884685E-9	45.0	9
TATATCG	20	7.02558E-4	45.0	21
ATTCGGG	20	7.02558E-4	45.0	3
TCGATAG	35	1.2082273E-7	45.0	1
>>END_MODULE