FastQCFastQC Report
Sat 18 Jun 2016
SRR3553900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784249
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.19738628930352475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14090.17966232663350543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGA207.0319144E-445.016
CGTTGTC207.0319144E-445.036
CCGTCGA253.8896687E-545.041
GTATGCG253.8896687E-545.01
CGCAAAC207.0319144E-445.08
CATGCGG650.041.538462
TATAGCG650.041.538461
CACGACC2500.040.49999627
TTACCGG451.9270374E-840.02
TATGCGG1100.038.863642
CGACGGG1750.038.571433
CGCATCG356.245753E-638.57142621
TAACGCC356.245753E-638.57142612
CGACACC356.245753E-638.57142635
CTACGAA356.245753E-638.57142611
ACGACTA356.245753E-638.57142642
CGTTAGG659.094947E-1238.076922
TAATGCG659.094947E-1238.076921
TCGTTGA601.546141E-1037.50000424
GTCGAAT301.13959104E-437.50000443