##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810720
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14715808170515	33.0	31.0	34.0	30.0	34.0
2	32.32159315176633	34.0	31.0	34.0	30.0	34.0
3	32.324024324057625	34.0	31.0	34.0	30.0	34.0
4	35.85949526346951	37.0	35.0	37.0	35.0	37.0
5	35.77725478586935	37.0	35.0	37.0	35.0	37.0
6	35.74955965068088	37.0	35.0	37.0	35.0	37.0
7	35.92558219853957	37.0	35.0	37.0	35.0	37.0
8	35.856742155121374	37.0	35.0	37.0	35.0	37.0
9	37.63935020722321	39.0	38.0	39.0	35.0	39.0
10	37.05690990724295	39.0	37.0	39.0	33.0	39.0
11	36.981777925794354	39.0	37.0	39.0	33.0	39.0
12	36.916919528320506	39.0	37.0	39.0	33.0	39.0
13	36.908295095717385	39.0	37.0	39.0	33.0	39.0
14	38.1027210380896	40.0	37.0	41.0	33.0	41.0
15	38.18210109532268	40.0	37.0	41.0	33.0	41.0
16	38.24897868561279	40.0	37.0	41.0	34.0	41.0
17	38.17712527136373	40.0	37.0	41.0	33.0	41.0
18	38.12427348529702	40.0	37.0	41.0	33.0	41.0
19	38.03395623643181	40.0	37.0	41.0	34.0	41.0
20	37.96189066508782	40.0	36.0	41.0	33.0	41.0
21	37.85924240181567	40.0	36.0	41.0	33.0	41.0
22	37.80785474639826	40.0	36.0	41.0	33.0	41.0
23	37.75567150187488	40.0	36.0	41.0	33.0	41.0
24	37.723440892046575	40.0	36.0	41.0	33.0	41.0
25	37.661293664890465	40.0	36.0	41.0	33.0	41.0
26	37.58393896783107	40.0	36.0	41.0	33.0	41.0
27	37.53601366686402	40.0	36.0	41.0	33.0	41.0
28	37.43295465758832	40.0	35.0	41.0	33.0	41.0
29	37.35274447404776	39.0	35.0	41.0	32.0	41.0
30	37.25441336096309	39.0	35.0	41.0	32.0	41.0
31	37.14831137754095	39.0	35.0	41.0	32.0	41.0
32	37.029091424906255	39.0	35.0	41.0	31.0	41.0
33	36.899052693901716	39.0	35.0	41.0	31.0	41.0
34	36.775398411288734	39.0	35.0	41.0	31.0	41.0
35	36.62544158279061	39.0	35.0	41.0	30.0	41.0
36	36.51841449575686	39.0	35.0	41.0	30.0	41.0
37	36.43444592461022	39.0	35.0	41.0	30.0	41.0
38	36.33958333333333	39.0	35.0	41.0	30.0	41.0
39	36.29485642391948	39.0	35.0	41.0	30.0	41.0
40	36.18756167357411	39.0	35.0	41.0	30.0	41.0
41	36.0524583086639	39.0	35.0	41.0	29.0	41.0
42	35.96488676731794	39.0	35.0	40.0	29.0	41.0
43	35.83255871324255	38.0	35.0	40.0	28.0	41.0
44	35.739320603907636	38.0	35.0	40.0	28.0	41.0
45	35.672742747187684	38.0	35.0	40.0	28.0	41.0
46	35.56394069469114	38.0	35.0	40.0	28.0	41.0
47	35.44830767712651	38.0	34.0	40.0	27.0	41.0
48	35.31120732188672	38.0	34.0	40.0	27.0	41.0
49	35.23809453325439	38.0	34.0	40.0	27.0	41.0
50	35.14048623445826	38.0	34.0	40.0	26.0	41.0
51	33.7697515788435	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	6.0
11	5.0
12	10.0
13	14.0
14	24.0
15	51.0
16	74.0
17	182.0
18	327.0
19	582.0
20	978.0
21	1565.0
22	2331.0
23	3435.0
24	4685.0
25	6152.0
26	7927.0
27	9132.0
28	10236.0
29	11935.0
30	14388.0
31	18236.0
32	22859.0
33	31185.0
34	51150.0
35	63745.0
36	65603.0
37	94928.0
38	159561.0
39	229350.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74069962502467	21.297858693507006	25.193655022695875	12.767786658772447
2	33.49134103019538	23.742105782514308	25.04835208209986	17.71820110519045
3	30.96173771462404	25.282711663706337	27.687364318136964	16.068186303532663
4	28.146215709492793	27.077659364515494	26.579953621472274	18.19617130451944
5	24.60578251430827	31.762260706532462	25.51374087231103	18.118215906848235
6	24.428409315176633	36.84737023880008	24.598134991119007	14.126085454904283
7	83.0914495756858	6.107287349516479	7.3160894020130245	3.4851736727846854
8	84.89786856127887	5.028739885533846	6.738701401223604	3.3346901519636867
9	79.1058565225972	7.481744622064338	8.519710874284586	4.892687981053879
10	38.31261101243339	27.357534043812908	17.975503256364714	16.354351687388988
11	30.411239392145255	28.057159068482335	22.477920860469705	19.053680678902705
12	26.40998125123347	25.09436056838366	28.764308269192817	19.73134991119005
13	24.582346556147623	26.42465956187093	29.205150976909415	19.787842905072033
14	22.342855733175448	28.721630155910795	28.468645154924015	20.466868955989735
15	21.775828892835996	27.068655022695875	31.343003749753308	19.81251233471482
16	23.874950661140716	25.20105585158871	30.663977698835605	20.26001578843497
17	23.576943951055853	25.366587724491808	27.969952634695083	23.086515689757253
18	24.343423130057232	25.504119794750345	28.24822380106572	21.904233274126703
19	24.865921649891455	27.982904085257548	26.379021117031776	20.77215314781922
20	26.644834221432802	26.250986777185716	26.757450167752122	20.346728833629367
21	25.77708703374778	26.600182553779355	27.622360371028222	20.000370041444643
22	23.87359384251036	25.82161535425301	26.40998125123347	23.894809552003156
23	23.20122853759621	27.73867673179396	26.94160746003552	22.118487270574306
24	22.198539569765146	27.230363134004342	28.6800621669627	21.89103512926781
25	23.04124728636274	27.57018452733373	27.217658377738307	22.170909808565227
26	21.994276692322874	28.071837379119795	27.395154924018154	22.538731004539176
27	22.926411091375567	27.890393724097102	27.447824156305504	21.735371028221827
28	21.837009078350107	28.30335997631735	28.528221827511345	21.331409117821195
29	22.75113479376357	26.819000394710873	27.95317742253799	22.476687388987568
30	22.641232484704954	26.684058614564833	27.956631142687982	22.718077758042234
31	23.102057430432207	28.61838859285573	25.980733175448982	22.298820801263076
32	23.14880599960529	27.30671501874877	27.19792283402408	22.346556147621868
33	23.251307479771068	27.270574304322082	25.84961515689757	23.628503059009276
34	22.156478192224196	27.785301953818824	27.618043220840732	22.440176633116245
35	22.83735445036511	26.360149003355044	27.38960430234853	23.41289224393132
36	23.49319123741859	28.713859285573317	25.592189658575094	22.200759818432996
37	22.978463587921848	28.20936944937833	26.97602131438721	21.83614564831261
38	22.436229524373395	28.157070258535622	26.8449032958358	22.56179692125518
39	22.76803335306888	26.205471679494767	27.75693210972962	23.26956285770673
40	23.509596408131046	26.234581606473256	28.82857213341228	21.427249851983422
41	21.814929938819812	26.57452634695086	28.237985987763963	23.372557726465367
42	23.327412670219065	27.71820110519045	26.84354647720545	22.110839747385043
43	22.754835208209986	26.39295934477995	28.18173968817841	22.670465758831657
44	22.410449970396684	26.90262976119992	27.103068877047566	23.583851391355832
45	22.839204657588315	26.37532070258536	27.146857114663508	23.638617525162818
46	22.612739293467534	27.032514308269196	27.42192125518058	22.93282514308269
47	22.502960331557134	26.956162423524766	28.466671600552594	22.074205644365502
48	22.26884744424709	27.14315670021709	27.18361456483126	23.40438129070456
49	22.537004144464177	26.733520820998617	28.308910597986976	22.42056443655023
50	21.457223208999405	26.831581803828698	28.455693704361558	23.255501282810343
51	21.22138346161437	26.869942766923227	27.04645253601737	24.862221235445038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2118.0
1	2175.0
2	2232.0
3	3592.0
4	4952.0
5	3549.0
6	2146.0
7	2208.0
8	2270.0
9	2358.0
10	2446.0
11	2504.5
12	2563.0
13	2637.5
14	2712.0
15	2661.0
16	2610.0
17	2661.5
18	2713.0
19	2774.5
20	2836.0
21	3216.5
22	3597.0
23	3695.0
24	3793.0
25	4980.0
26	7310.5
27	8454.0
28	10085.5
29	11717.0
30	13472.5
31	15228.0
32	16040.5
33	16853.0
34	19091.0
35	21329.0
36	24027.0
37	26725.0
38	28768.5
39	30812.0
40	34251.5
41	37691.0
42	41214.0
43	44737.0
44	48174.5
45	51612.0
46	56091.5
47	60571.0
48	63171.0
49	65771.0
50	65243.0
51	64715.0
52	59821.0
53	54927.0
54	51065.0
55	47203.0
56	43681.5
57	40160.0
58	38919.0
59	37678.0
60	35196.0
61	32714.0
62	30441.0
63	28168.0
64	24296.5
65	20425.0
66	17520.0
67	14615.0
68	12310.0
69	10005.0
70	8610.5
71	7216.0
72	6791.5
73	6367.0
74	5177.0
75	3794.0
76	3601.0
77	2642.0
78	1683.0
79	1478.0
80	1273.0
81	1054.5
82	836.0
83	583.0
84	330.0
85	201.0
86	72.0
87	53.0
88	34.0
89	27.5
90	21.0
91	21.0
92	21.0
93	12.5
94	4.0
95	6.0
96	8.0
97	4.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	810720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.96856426194102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86569920703351	20.190204200800302
2	8.57696022960134	4.626166062482324
3	3.2221565147069837	2.606908050867216
4	1.7772682901435837	1.9172149637378912
5	1.1970585496195219	1.6141475210359997
6	0.8925785989716631	1.444293798312035
7	0.7075607350286682	1.3357328006292806
8	0.6148725602167995	1.3265784122488766
9	0.5320322930758398	1.2913332376719262
>10	6.477802998460641	41.00861607193728
>50	1.064721944769251	18.61189583348713
>100	0.06668884750943094	2.805663852596083
>500	0.0036793846901755	0.6944700225767229
>1k	9.19846172543875E-4	0.5267751716169569
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2359	0.2909759226366686	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1888	0.23287941582790608	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.10990230905861456	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCC	868	0.1070653246496941	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4669429642786657E-4	0.0	0.0	0.06969113874087231	0.0
2	2.4669429642786657E-4	0.0	0.0	0.33007696862048547	0.0
3	2.4669429642786657E-4	0.0	0.0	0.46871916321294654	0.0
4	2.4669429642786657E-4	0.0	0.0	0.6464624037892244	0.0
5	2.4669429642786657E-4	0.0	0.0	1.2375419380303927	0.0
6	2.4669429642786657E-4	0.0	0.0	1.6675300967041642	0.0
7	2.4669429642786657E-4	0.0	0.0	2.0444789816459443	0.0
8	2.4669429642786657E-4	0.0	0.0	2.5933737911979473	0.0
9	2.4669429642786657E-4	0.0	0.0	2.8522794552989934	0.0
10	2.4669429642786657E-4	0.0	0.0	3.4438523781330175	0.0
11	2.4669429642786657E-4	0.0	0.0	4.229820406552201	0.0
12	2.4669429642786657E-4	0.0	0.0	4.8191730807183735	0.0
13	2.4669429642786657E-4	0.0	0.0	5.061303532662325	0.0
14	2.4669429642786657E-4	0.0	0.0	5.172809354647721	0.0
15	3.700414446417999E-4	0.0	0.0	5.346358792184724	0.0
16	3.700414446417999E-4	0.0	0.0	5.74069962502467	0.0
17	3.700414446417999E-4	0.0	0.0	6.215586145648313	0.0
18	3.700414446417999E-4	0.0	0.0	6.765097690941386	0.0
19	3.700414446417999E-4	0.0	0.0	7.066434774028025	0.0
20	3.700414446417999E-4	0.0	0.0	7.3380451943951055	0.0
21	3.700414446417999E-4	0.0	0.0	7.6797167949477005	0.0
22	4.933885928557331E-4	0.0	0.0	8.053705348332347	0.0
23	4.933885928557331E-4	0.0	0.0	8.436574896388395	0.0
24	4.933885928557331E-4	0.0	0.0	8.743585948292875	0.0
25	4.933885928557331E-4	0.0	0.0	9.018280047365305	0.0
26	6.167357410696664E-4	0.0	0.0	9.266947898164595	0.0
27	6.167357410696664E-4	0.0	0.0	9.528567199526346	0.0
28	7.400828892835998E-4	0.0	0.0	9.81621274916124	0.0
29	7.400828892835998E-4	0.0	0.0	10.102378133017565	0.0
30	8.634300374975331E-4	0.0	0.0	10.419873692520229	0.0
31	8.634300374975331E-4	0.0	0.0	10.7347789619104	0.0
32	8.634300374975331E-4	0.0	0.0	11.021684428656009	0.0
33	8.634300374975331E-4	0.0	0.0	11.311673574106967	0.0
34	8.634300374975331E-4	0.0	0.0	11.584764160252615	0.0
35	8.634300374975331E-4	0.0	0.0	11.880180580224986	0.0
36	8.634300374975331E-4	0.0	0.0	12.163632326820604	0.0
37	8.634300374975331E-4	0.0	0.0	12.459172093941188	0.0
38	8.634300374975331E-4	0.0	0.0	12.74620090783501	0.0
39	8.634300374975331E-4	0.0	0.0	13.037916913360963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTGA	20	7.032056E-4	45.0	18
GGTCGAT	20	7.032056E-4	45.0	8
ACGTTAG	20	7.032056E-4	45.0	1
CTATCGC	25	3.889785E-5	45.0	25
AAGCGAT	20	7.032056E-4	45.0	34
CGACACG	20	7.032056E-4	45.0	18
CATTCGC	25	3.889785E-5	45.0	26
ATCGTAG	35	1.2113378E-7	45.0	1
CCCGTCA	20	7.032056E-4	45.0	12
CCCGTAG	20	7.032056E-4	45.0	30
TGACCGG	20	7.032056E-4	45.0	2
CATACGA	105	0.0	45.0	18
GCGATAT	40	6.8102963E-9	45.0	9
GTATACG	25	3.889785E-5	45.0	1
CGCGTTA	20	7.032056E-4	45.0	30
ATACGCA	20	7.032056E-4	45.0	12
CGATACG	20	7.032056E-4	45.0	10
AATACGC	25	3.889785E-5	45.0	11
GCGTACT	20	7.032056E-4	45.0	28
GCGTACG	40	6.8102963E-9	45.0	1
>>END_MODULE