FastQCFastQC Report
Sat 18 Jun 2016
SRR3553897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741702
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15200.2049340570741349No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.18511477655446526No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.10246702853706745No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7540.10165807831177481No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA207.031666E-445.035
GCGAAGT207.031666E-445.044
CGTTAAC207.031666E-445.042
TACGAAT900.045.012
GCGATAT406.8102963E-945.09
CGGTCTA207.031666E-445.031
TCGACGA253.889463E-545.022
TACAACG207.031666E-445.042
AAATGCG207.031666E-445.01
TATACGG650.041.538462
TATCACG501.0804797E-940.51
CTACGAA1000.040.511
CGAATAT1000.040.514
AGCTACG1000.040.59
GCTTGCG451.9266736E-840.01
GCGAACA403.456007E-739.3759
TAGGTTG403.456007E-739.3751
CGCAAAC403.456007E-739.37520
ACGGGTA1150.039.1304325
AGGCGAT356.2452928E-638.5714266