##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553897_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741702
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19195849546044	33.0	31.0	34.0	30.0	34.0
2	32.34584644506823	34.0	31.0	34.0	30.0	34.0
3	32.36698296620476	34.0	31.0	34.0	30.0	34.0
4	35.89039263747435	37.0	35.0	37.0	35.0	37.0
5	35.807637029426914	37.0	35.0	37.0	35.0	37.0
6	35.77000601319668	37.0	35.0	37.0	35.0	37.0
7	35.92438068119002	37.0	35.0	37.0	35.0	37.0
8	35.80925762637825	37.0	35.0	37.0	35.0	37.0
9	37.624059258300505	39.0	37.0	39.0	35.0	39.0
10	37.11467813218786	39.0	37.0	39.0	33.0	39.0
11	37.00550625453349	39.0	37.0	39.0	33.0	39.0
12	36.85880313117667	39.0	37.0	39.0	33.0	39.0
13	36.79114657908432	39.0	37.0	39.0	33.0	39.0
14	38.001959007795584	40.0	37.0	41.0	33.0	41.0
15	38.10354023583596	40.0	37.0	41.0	33.0	41.0
16	38.15015194781732	40.0	37.0	41.0	33.0	41.0
17	38.08430609597925	40.0	37.0	41.0	33.0	41.0
18	38.03373457264508	40.0	37.0	41.0	33.0	41.0
19	37.98052047857495	40.0	37.0	41.0	33.0	41.0
20	37.8985414627438	40.0	36.0	41.0	33.0	41.0
21	37.81220624994944	40.0	36.0	41.0	33.0	41.0
22	37.76593834181383	40.0	36.0	41.0	33.0	41.0
23	37.66773448096405	40.0	36.0	41.0	33.0	41.0
24	37.627696837813566	40.0	36.0	41.0	33.0	41.0
25	37.55482390501846	40.0	35.0	41.0	33.0	41.0
26	37.47503175129634	39.0	35.0	41.0	33.0	41.0
27	37.40817875642778	39.0	35.0	41.0	33.0	41.0
28	37.31375269313013	39.0	35.0	41.0	32.0	41.0
29	37.23115483037662	39.0	35.0	41.0	32.0	41.0
30	37.11828739844304	39.0	35.0	41.0	32.0	41.0
31	37.00628554325053	39.0	35.0	41.0	31.0	41.0
32	36.880678493518964	39.0	35.0	41.0	31.0	41.0
33	36.7765719925253	39.0	35.0	41.0	31.0	41.0
34	36.65159187921834	39.0	35.0	41.0	31.0	41.0
35	36.503617355757434	39.0	35.0	41.0	30.0	41.0
36	36.39749522045242	39.0	35.0	41.0	30.0	41.0
37	36.315357111076956	39.0	35.0	41.0	30.0	41.0
38	36.18324070853254	39.0	35.0	41.0	30.0	41.0
39	36.108393937187714	39.0	35.0	41.0	29.0	41.0
40	36.00448428074887	38.0	35.0	40.0	29.0	41.0
41	35.856458523773696	38.0	35.0	40.0	29.0	41.0
42	35.76067881709905	38.0	35.0	40.0	28.0	41.0
43	35.65693769195715	38.0	35.0	40.0	28.0	41.0
44	35.52919771013156	38.0	34.0	40.0	28.0	41.0
45	35.4541163432214	38.0	34.0	40.0	27.0	41.0
46	35.3010319508374	38.0	34.0	40.0	27.0	41.0
47	35.20098772822508	37.0	34.0	40.0	27.0	41.0
48	35.07994046126342	37.0	34.0	40.0	26.0	41.0
49	34.98695972236828	37.0	34.0	40.0	26.0	41.0
50	34.88484728367997	37.0	34.0	40.0	26.0	41.0
51	33.527935747780106	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	7.0
12	15.0
13	11.0
14	29.0
15	61.0
16	89.0
17	196.0
18	349.0
19	597.0
20	1045.0
21	1579.0
22	2398.0
23	3156.0
24	4423.0
25	5536.0
26	6821.0
27	8252.0
28	9506.0
29	11543.0
30	13658.0
31	17101.0
32	22189.0
33	30163.0
34	51628.0
35	61808.0
36	61306.0
37	87393.0
38	139322.0
39	201427.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.085632774348724	20.986865344842	25.218483973347787	10.709017907461488
2	32.27751846428889	24.425982402636098	25.148105303747325	18.14839382932768
3	32.741046943381576	24.682149973978767	25.62228496080636	16.9545181218333
4	27.996284221965155	26.64762937136478	25.68888852935546	19.66719787731461
5	24.888836756541036	31.836910241579503	23.83261741238395	19.44163558949551
6	25.315558000382904	36.16452429682002	23.98429557962632	14.53562212317076
7	83.26929144049767	6.0548576112778445	6.904524997910213	3.771325950314277
8	84.38726065185209	5.5860709557207615	6.305901831193659	3.720766561233487
9	79.1350164891021	7.544539451154238	7.914634179225619	5.405809880518052
10	41.67374498113798	26.51051230817768	15.672601664819563	16.14314104586478
11	32.555123216601814	26.23776125721651	21.554748402997433	19.652367123184245
12	28.081223995620885	23.716533055054455	28.00127274835446	20.2009702009702
13	24.895308358343378	25.55136699105571	28.315819560955745	21.23750508964517
14	21.660046757323023	28.1946118521994	27.340360414290377	22.804980976187203
15	21.3332308663048	25.53330043602417	31.10332721227663	22.030141485394402
16	23.943443593249043	23.8709077230478	30.607036249059593	21.578612434643563
17	24.408994447904952	23.292373486926017	27.615538315927424	24.683093749241607
18	24.470070189914548	24.71140700712685	27.78460891301358	23.03391388994502
19	25.84151047186067	26.8270814963422	25.03916667340792	22.292241358389216
20	27.35182054248202	25.051840226937504	25.165767383666214	22.430571846914262
21	25.532221835723785	26.366788818150688	26.09835216839108	22.002637177734456
22	24.273899760281083	24.81468298588921	25.234662977853638	25.676754275976066
23	23.986857255339746	27.004241595681282	25.76749152624639	23.241409622732583
24	23.18100800591073	26.02676007345268	27.60906671412508	23.18316520651151
25	24.062494101404607	26.226166303987313	26.177224815357107	23.534114779250967
26	22.95005271658968	26.85108035302588	26.344003386804943	23.854863543579498
27	22.455379653823236	25.75373937241642	28.358693922896254	23.432187050864094
28	22.30194876109273	26.08379106433581	28.80779612297122	22.80646405160024
29	23.55892258615994	25.848386548775654	26.860652930691842	23.732037934372567
30	23.52899142782411	25.31191772436909	26.804430890034002	24.3546599577728
31	24.874545302560865	26.418291982494317	25.399553998775787	23.307608716169028
32	25.33591658105277	26.548802618841528	25.64749724282798	22.46778355727772
33	24.849467845576793	26.450110691355828	24.486114369382854	24.214307093684525
34	23.070451475120734	25.975526559184146	26.513478459003753	24.440543506691366
35	23.02299306190357	25.902855863945355	26.37717034604194	24.696980728109132
36	24.53155040703679	27.066800413103913	25.22455110003748	23.177098079821814
37	23.425580624024203	27.606235388336554	25.829645868556373	23.138538119082867
38	24.292775265537912	27.319597358507863	25.886811684477056	22.500815691477168
39	24.290483239899583	25.91472046724965	26.08729651531208	23.707499777538686
40	24.363693235288565	24.843131068812003	28.260406470523204	22.532769225376228
41	21.60638639237861	25.868071004257775	27.644121223887762	24.881421379475853
42	23.07786685218592	26.11965452432379	26.462379769772763	24.34009885371753
43	23.08784390496453	25.261627985363393	26.62443946490639	25.026088644765686
44	23.2060854628948	25.45658498965892	26.755219751328703	24.582109796117578
45	24.00964268668549	25.881283857937554	25.977818584822476	24.131254870554482
46	22.71599645140501	26.752927725690373	27.07097998926793	23.46009583363669
47	23.014229434462898	25.270661262879162	28.482328482328484	23.232780820329456
48	23.787855499917757	25.477348045441428	26.744298923287253	23.990497531353565
49	22.97459087342356	25.809422112924057	27.960555587014728	23.255431426637653
50	21.94371863632564	25.687000978829772	28.33091996516121	24.03836041968338
51	22.562970033787156	25.211607896432795	26.577924826952064	25.64749724282798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1679.0
1	1963.0
2	2247.0
3	2764.0
4	3281.0
5	2511.0
6	1741.0
7	1745.5
8	1750.0
9	1816.0
10	1882.0
11	1992.0
12	2102.0
13	2144.5
14	2187.0
15	2108.0
16	2029.0
17	1875.0
18	1721.0
19	1861.0
20	2001.0
21	2141.5
22	2282.0
23	2791.0
24	3300.0
25	4391.0
26	5680.0
27	5878.0
28	7017.5
29	8157.0
30	9744.0
31	11331.0
32	13100.5
33	14870.0
34	17288.0
35	19706.0
36	21223.5
37	22741.0
38	23272.5
39	23804.0
40	26902.0
41	30000.0
42	33459.5
43	36919.0
44	39698.0
45	42477.0
46	47405.5
47	52334.0
48	52977.5
49	53621.0
50	53095.0
51	52569.0
52	49415.0
53	46261.0
54	45058.0
55	43855.0
56	42652.0
57	41449.0
58	41015.5
59	40582.0
60	38150.0
61	35718.0
62	33618.5
63	31519.0
64	28196.5
65	24874.0
66	21644.0
67	18414.0
68	16561.5
69	14709.0
70	13102.5
71	11496.0
72	10229.5
73	8963.0
74	7759.0
75	5144.0
76	3733.0
77	2995.0
78	2257.0
79	1825.0
80	1393.0
81	1175.5
82	958.0
83	719.0
84	480.0
85	332.0
86	184.0
87	148.5
88	113.0
89	70.0
90	27.0
91	23.5
92	20.0
93	29.5
94	39.0
95	25.5
96	12.0
97	6.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	741702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.325087001583608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35309795216205	17.576175655730314
2	8.537207889947988	3.982622335672857
3	2.951646528795408	2.0654223624622547
4	1.5252964750241473	1.4231069193258816
5	0.9645655514830558	1.1249287703536375
6	0.7691472921278945	1.076425650354935
7	0.6087212487167335	0.9938933260221297
8	0.5041331440716944	0.9407159556683327
9	0.42653974021387103	0.8954168895109271
>10	6.4656888516937645	37.31107269045666
>50	1.7643597327455005	27.35293783671865
>100	0.12320298529052899	4.097762556762566
>500	0.005230315413277174	0.7673646653600029
>1k	0.0011622923140615943	0.3921543856008635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1520	0.2049340570741349	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.18511477655446526	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	760	0.10246702853706745	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	754	0.10165807831177481	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6965007509754593E-4	0.0	0.0	0.053525539906862867	0.0
2	2.6965007509754593E-4	0.0	0.0	0.25751582171815635	0.0
3	2.6965007509754593E-4	0.0	0.0	0.36267935100619925	0.0
4	2.6965007509754593E-4	0.0	0.0	0.5239300959145317	0.0
5	2.6965007509754593E-4	0.0	0.0	1.0590506699456115	0.0
6	2.6965007509754593E-4	0.0	0.0	1.4144494689241771	0.0
7	2.6965007509754593E-4	0.0	0.0	1.7377599089661346	0.0
8	2.6965007509754593E-4	0.0	0.0	2.2402528239104114	0.0
9	2.6965007509754593E-4	0.0	0.0	2.4643320363164722	0.0
10	2.6965007509754593E-4	0.0	0.0	3.0283051683829894	0.0
11	2.6965007509754593E-4	0.0	0.0	3.6606345944867344	0.0
12	2.6965007509754593E-4	0.0	0.0	4.148431580338195	0.0
13	4.044751126463189E-4	0.0	0.0	4.342309984333331	0.0
14	4.044751126463189E-4	0.0	0.0	4.422261231599753	0.0
15	4.044751126463189E-4	0.0	0.0	4.56207479553783	0.0
16	5.393001501950919E-4	0.0	0.0	4.8578809279198385	0.0
17	5.393001501950919E-4	0.0	0.0	5.219346853588099	0.0
18	5.393001501950919E-4	0.0	0.0	5.642158171341051	0.0
19	5.393001501950919E-4	0.0	0.0	5.851541454654295	0.0
20	5.393001501950919E-4	0.0	0.0	6.081957443825148	0.0
21	5.393001501950919E-4	0.0	0.0	6.342709066444475	0.0
22	5.393001501950919E-4	0.0	0.0	6.61572976748074	0.0
23	5.393001501950919E-4	0.0	0.0	6.902098147234334	0.0
24	5.393001501950919E-4	0.0	0.0	7.152198591887307	0.0
25	5.393001501950919E-4	0.0	0.0	7.361716700238101	0.0
26	5.393001501950919E-4	0.0	0.0	7.570021383250955	0.0
27	5.393001501950919E-4	0.0	0.0	7.761203286495116	0.0
28	6.741251877438648E-4	0.0	0.0	7.965193568306408	0.0
29	6.741251877438648E-4	0.0	0.0	8.18590215477375	0.0
30	8.089502252926377E-4	0.0	0.0	8.42980064769948	0.0
31	8.089502252926377E-4	0.0	0.0	8.65387986010554	0.0
32	8.089502252926377E-4	0.0	0.0	8.884295849276395	0.0
33	9.437752628414107E-4	0.0	0.0	9.1198351898741	0.0
34	9.437752628414107E-4	0.0	0.0	9.364138157912477	0.0
35	9.437752628414107E-4	0.0	0.0	9.621114679480439	0.0
36	9.437752628414107E-4	0.0	0.0	9.850047593238255	0.0
37	9.437752628414107E-4	0.0	0.0	10.082755608047437	0.0
38	9.437752628414107E-4	0.0	0.0	10.306969645491046	0.0
39	9.437752628414107E-4	0.0	0.0	10.559092465707252	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	20	7.031666E-4	45.0	35
GCGAAGT	20	7.031666E-4	45.0	44
CGTTAAC	20	7.031666E-4	45.0	42
TACGAAT	90	0.0	45.0	12
GCGATAT	40	6.8102963E-9	45.0	9
CGGTCTA	20	7.031666E-4	45.0	31
TCGACGA	25	3.889463E-5	45.0	22
TACAACG	20	7.031666E-4	45.0	42
AAATGCG	20	7.031666E-4	45.0	1
TATACGG	65	0.0	41.53846	2
TATCACG	50	1.0804797E-9	40.5	1
CTACGAA	100	0.0	40.5	11
CGAATAT	100	0.0	40.5	14
AGCTACG	100	0.0	40.5	9
GCTTGCG	45	1.9266736E-8	40.0	1
GCGAACA	40	3.456007E-7	39.375	9
TAGGTTG	40	3.456007E-7	39.375	1
CGCAAAC	40	3.456007E-7	39.375	20
ACGGGTA	115	0.0	39.130432	5
AGGCGAT	35	6.2452928E-6	38.571426	6
>>END_MODULE