SRR3553894_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594544
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2169	0.36481740628111625	No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1887	0.3173860975806669	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	1505	0.2531351758658737	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1390	0.23379262089937833	RNA PCR Primer, Index 29 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	1255	0.21108614333001427	RNA PCR Primer, Index 29 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	1219	0.2050310826448505	RNA PCR Primer, Index 29 (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	960	0.16146828493770016	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	875	0.14717161387550795	No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	734	0.12345595952528324	Illumina PCR Primer Index 8 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	690	0.11605532979897198	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	689	0.11588713366882855	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	684	0.11504615301811136	No Hit
AAAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	612	0.10293603164778385	RNA PCR Primer, Index 29 (95% over 22bp)
TGTTGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCT	596	0.10024489356548884	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAAC	25	3.888525E-5	45.000004	42
CGTTCTA	45	3.8380676E-10	45.000004	27
CGTTAGG	30	2.163726E-6	45.000004	2
ATAACGC	45	3.8380676E-10	45.000004	11
CACCGTA	30	2.163726E-6	45.000004	25
TCGACAA	30	2.163726E-6	45.000004	19
CGGGTTA	20	7.030536E-4	45.0	6
TTAGGCG	20	7.030536E-4	45.0	1
TTAGCGA	20	7.030536E-4	45.0	26
TTGTCGC	20	7.030536E-4	45.0	38
TGACGGA	20	7.030536E-4	45.0	34
TCGATCT	20	7.030536E-4	45.0	16
TAGCCGT	20	7.030536E-4	45.0	44
CCGTACA	20	7.030536E-4	45.0	28
CTACGCA	80	0.0	45.0	15
CCGAACA	20	7.030536E-4	45.0	11
GCCGTAC	20	7.030536E-4	45.0	43
TACGTAG	35	1.2106102E-7	45.0	1
GTACGAC	20	7.030536E-4	45.0	35
AGCACGT	20	7.030536E-4	45.0	34