##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594544
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955929249979818	33.0	31.0	34.0	30.0	34.0
2	32.112454250652604	33.0	31.0	34.0	30.0	34.0
3	32.14290447805377	34.0	31.0	34.0	30.0	34.0
4	35.70301272908313	37.0	35.0	37.0	33.0	37.0
5	35.616272302806856	37.0	35.0	37.0	33.0	37.0
6	35.58629470653139	37.0	35.0	37.0	33.0	37.0
7	35.772317944508735	37.0	35.0	37.0	35.0	37.0
8	35.6466905729433	37.0	35.0	37.0	35.0	37.0
9	37.44391331844237	39.0	37.0	39.0	35.0	39.0
10	36.90986032993353	39.0	37.0	39.0	33.0	39.0
11	36.810873543421515	39.0	37.0	39.0	32.0	39.0
12	36.69088074221588	39.0	35.0	39.0	33.0	39.0
13	36.59373738529024	39.0	35.0	39.0	32.0	39.0
14	37.64166150865201	40.0	36.0	41.0	33.0	41.0
15	37.75736362657768	40.0	36.0	41.0	33.0	41.0
16	37.85579368389892	40.0	36.0	41.0	33.0	41.0
17	37.79164199790091	40.0	36.0	41.0	33.0	41.0
18	37.74321160418741	39.0	36.0	41.0	33.0	41.0
19	37.668858822896205	39.0	36.0	41.0	33.0	41.0
20	37.58778997012837	39.0	35.0	41.0	33.0	41.0
21	37.49925825506607	39.0	35.0	41.0	33.0	41.0
22	37.45170248122931	39.0	35.0	41.0	33.0	41.0
23	37.368063591592886	39.0	35.0	41.0	32.0	41.0
24	37.33749899082322	39.0	35.0	41.0	32.0	41.0
25	37.269376194192525	39.0	35.0	41.0	32.0	41.0
26	37.19930064049086	39.0	35.0	41.0	32.0	41.0
27	37.12088424069539	39.0	35.0	41.0	32.0	41.0
28	37.06064143276191	39.0	35.0	41.0	32.0	41.0
29	36.9750228746737	39.0	35.0	41.0	32.0	41.0
30	36.80725564466213	39.0	35.0	41.0	31.0	41.0
31	36.71883998492962	39.0	35.0	41.0	31.0	41.0
32	36.593222705132	39.0	35.0	41.0	31.0	41.0
33	36.4514585968406	39.0	35.0	40.0	30.0	41.0
34	36.34864030248392	39.0	35.0	40.0	30.0	41.0
35	36.178286215990745	39.0	35.0	40.0	30.0	41.0
36	36.085645469469036	38.0	35.0	40.0	30.0	41.0
37	36.004147716569335	38.0	35.0	40.0	30.0	41.0
38	35.96330465028661	38.0	35.0	40.0	29.0	41.0
39	35.869801057617266	38.0	35.0	40.0	29.0	41.0
40	35.73161784493662	38.0	35.0	40.0	29.0	41.0
41	35.618450442692215	38.0	35.0	40.0	28.0	41.0
42	35.53446675098899	38.0	34.0	40.0	28.0	41.0
43	35.430926895233995	38.0	34.0	40.0	27.0	41.0
44	35.32038503458112	38.0	34.0	40.0	27.0	41.0
45	35.232605156220565	38.0	34.0	40.0	27.0	41.0
46	35.10218251298474	37.0	34.0	40.0	26.0	41.0
47	34.98903697623725	37.0	34.0	40.0	26.0	41.0
48	34.87431880567292	37.0	34.0	40.0	26.0	41.0
49	34.81684955192551	37.0	34.0	40.0	26.0	41.0
50	34.71962377889609	37.0	34.0	40.0	26.0	41.0
51	33.3246942194354	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	1.0
11	3.0
12	7.0
13	13.0
14	19.0
15	51.0
16	99.0
17	144.0
18	333.0
19	534.0
20	902.0
21	1339.0
22	2043.0
23	2772.0
24	3741.0
25	4762.0
26	5905.0
27	6935.0
28	8113.0
29	9775.0
30	12227.0
31	15320.0
32	19832.0
33	27401.0
34	45153.0
35	54499.0
36	51501.0
37	71420.0
38	112771.0
39	136881.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10808956107538	20.90492881939772	23.92993622002745	11.057045399499449
2	32.77082940875696	25.38247799994618	23.411387550795233	18.43530504050163
3	32.15169945369897	25.976714927742943	25.695490998143118	16.176094620414975
4	28.53968755886865	26.656731881912858	26.16509459350359	18.638485965714903
5	23.95348367824753	31.66796738340644	24.067184262224494	20.31136467612153
6	24.47590085847305	37.03476950402325	24.301986059904735	14.187343577598966
7	80.21660297639872	6.835827121289594	8.283491213434163	4.6640786888775265
8	80.84986140638875	6.317951236577948	7.559911461557093	5.272275895476197
9	75.497187760704	7.666211415807746	10.221110632686562	6.61549019080169
10	40.60725530826987	24.596161091525605	17.55765763341317	17.238925966791356
11	31.176161898867033	25.931806560994648	22.561492505180443	20.330539034957884
12	28.04182701364407	22.25217982184666	28.862792324874185	20.84320083963508
13	25.476836028956644	24.688668963104497	30.24418714174224	19.590307866196614
14	20.680555181786378	29.33054576280309	28.92586587367798	21.063033181732553
15	20.288658198552167	26.51797007454452	33.32688581501117	19.86648591189214
16	22.22106353777012	24.531405581420383	30.95296563416669	22.294565246642804
17	22.115099975779756	24.2720471487392	27.4041954842703	26.208657391210743
18	23.380775855109125	24.781513226943673	28.946217605425336	22.891493312521867
19	23.995700906913534	27.5323609354396	25.53553647837671	22.936401679270162
20	24.406940445114238	26.91894964880648	26.329422212653732	22.34468769342555
21	24.075089480341237	28.044686347856505	27.301595844882804	20.578628326919453
22	22.431476896579564	25.14027557253963	26.5019914421809	25.926256088699912
23	21.349471191366828	27.67616862671224	26.438581501116822	24.535778680804114
24	23.033955434753356	25.57657633413171	28.13517586587368	23.254292365241263
25	22.575284587852202	26.424284830054628	26.653199763179845	24.347230818913317
26	20.11306143868242	26.23556877203369	27.594257111332382	26.057112677951505
27	21.848677305632553	26.994133318980595	26.45506472187088	24.702124653515973
28	20.66743588363519	26.32403993648914	28.26401410156355	24.744510078312118
29	21.66315697408434	26.19133318980597	26.955280012917466	25.19022982319223
30	22.4459417637719	27.612254097257733	26.69003471568126	23.251769423289108
31	23.45394117172152	27.406382033962164	23.891082913964315	25.248593880352
32	23.40398692106892	26.728215237223825	24.96484700880002	24.902950832907237
33	22.65299119997847	27.0530019645308	24.16759735192013	26.1264094835706
34	20.832604483436047	26.374498775532174	26.729392610134827	26.06350413089696
35	21.08977636642536	26.200920369224146	25.703396216259854	27.005907048090638
36	22.068509647730025	28.48165989396916	24.17163405904357	25.278196399257247
37	20.761793912645658	28.621094485858066	26.186623698161952	24.43048790333432
38	21.396902500067277	28.73159934336231	24.17937108103017	25.692127075540245
39	22.514733981000564	26.425462202965633	25.56900750827525	25.49079630775855
40	22.79192120347695	25.26423612045534	26.742511908286016	25.201330767781695
41	19.98388681073226	26.071745741273983	25.889589332328644	28.054778115665112
42	21.66130681665276	25.36734034823327	26.119177049974436	26.852175785139536
43	21.6692120347695	24.25287278990285	27.385021125433944	26.692894049893702
44	21.300862509755376	24.936253666675636	27.71115342178207	26.051730401786916
45	22.350069969590137	25.186193116068782	26.387281681423076	26.076455232918
46	23.84348340913372	26.256761484431767	25.858641244382248	24.041113862052264
47	20.808552436825533	25.529313221561402	28.214396243171237	25.44773809844183
48	21.254440377835788	25.233792620899376	27.34953174197368	26.162235259291155
49	22.306338975752844	23.21022497914368	28.37754648941037	26.1058895556931
50	20.703429855485886	23.619446163782666	28.983893538577465	26.693230442153986
51	20.446762560887	24.023285072257057	26.61014155386313	28.919810812992814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2483.0
1	2417.0
2	2351.0
3	2433.5
4	2516.0
5	1925.0
6	1334.0
7	1434.0
8	1534.0
9	1667.0
10	1800.0
11	1873.5
12	1947.0
13	1948.0
14	1949.0
15	1944.0
16	1939.0
17	2069.0
18	2199.0
19	2122.5
20	2046.0
21	1935.5
22	1825.0
23	1948.5
24	2072.0
25	2514.5
26	3604.5
27	4252.0
28	4643.5
29	5035.0
30	5401.0
31	5767.0
32	6991.0
33	8215.0
34	9636.0
35	11057.0
36	12508.5
37	13960.0
38	15454.0
39	16948.0
40	19230.0
41	21512.0
42	24839.0
43	28166.0
44	34083.0
45	40000.0
46	45478.5
47	50957.0
48	54227.5
49	57498.0
50	56861.0
51	56224.0
52	50894.0
53	45564.0
54	41122.0
55	36680.0
56	33995.5
57	31311.0
58	29339.5
59	27368.0
60	25966.5
61	24565.0
62	22718.5
63	20872.0
64	17990.0
65	15108.0
66	13730.0
67	12352.0
68	10916.0
69	9480.0
70	8234.5
71	6989.0
72	6240.0
73	5491.0
74	4697.5
75	3340.0
76	2776.0
77	2215.5
78	1655.0
79	1314.0
80	973.0
81	749.5
82	526.0
83	350.5
84	175.0
85	132.5
86	90.0
87	65.0
88	40.0
89	35.0
90	30.0
91	29.0
92	28.0
93	18.0
94	8.0
95	11.0
96	14.0
97	8.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	594544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.103171308605223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75805763876583	21.009385005208195
2	9.909721053457307	5.569891767716052
3	3.740354632125293	3.153474809446566
4	1.8569172874123803	2.0874105853624263
5	1.0902530634871666	1.5319784306455748
6	0.7617876185325876	1.284518876663743
7	0.6079514945087393	1.195975549825117
8	0.48881222840330035	1.0989739034047212
9	0.38324967324543746	0.9693478099065154
>10	5.1620593523470575	34.85831610530486
>50	1.159154597478024	21.180762058707813
>100	0.0726723866812647	3.396335061084077
>500	0.00540538413331721	1.0728111344921176
>1k	0.0036035894222114727	1.5908189022322543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2169	0.36481740628111625	No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1887	0.3173860975806669	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	1505	0.2531351758658737	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1390	0.23379262089937833	RNA PCR Primer, Index 29 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	1255	0.21108614333001427	RNA PCR Primer, Index 29 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	1219	0.2050310826448505	RNA PCR Primer, Index 29 (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	960	0.16146828493770016	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	875	0.14717161387550795	No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	734	0.12345595952528324	Illumina PCR Primer Index 8 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	690	0.11605532979897198	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	689	0.11588713366882855	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	684	0.11504615301811136	No Hit
AAAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	612	0.10293603164778385	RNA PCR Primer, Index 29 (95% over 22bp)
TGTTGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCT	596	0.10024489356548884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6819613014343765E-4	0.0	0.0	0.21394547754245272	0.0
2	1.6819613014343765E-4	0.0	0.0	1.007326623429048	0.0
3	1.6819613014343765E-4	0.0	0.0	1.2981377324470518	0.0
4	1.6819613014343765E-4	0.0	0.0	1.742511908286014	0.0
5	1.6819613014343765E-4	0.0	0.0	3.2677144164267067	0.0
6	1.6819613014343765E-4	0.0	0.0	4.179673834064426	0.0
7	1.6819613014343765E-4	0.0	0.0	5.066572028310772	0.0
8	1.6819613014343765E-4	0.0	0.0	6.404235851341532	0.0
9	1.6819613014343765E-4	0.0	0.0	6.915215694717296	0.0
10	1.6819613014343765E-4	0.0	0.0	8.228491078877257	0.0
11	1.6819613014343765E-4	0.0	0.0	9.61173605317689	0.0
12	1.6819613014343765E-4	0.0	0.0	10.73713635996663	0.0
13	1.6819613014343765E-4	0.0	0.0	11.147030329126187	0.0
14	1.6819613014343765E-4	0.0	0.0	11.321786108345218	0.0
15	1.6819613014343765E-4	0.0	0.0	11.625211927123981	0.0
16	1.6819613014343765E-4	0.0	0.0	12.27007589009392	0.0
17	1.6819613014343765E-4	0.0	0.0	13.034359105465702	0.0
18	1.6819613014343765E-4	0.0	0.0	13.956746683172314	0.0
19	1.6819613014343765E-4	0.0	0.0	14.436442046341398	0.0
20	1.6819613014343765E-4	0.0	0.0	14.873751984714335	0.0
21	1.6819613014343765E-4	0.0	0.0	15.389441319734116	0.0
22	1.6819613014343765E-4	0.0	0.0	15.945329529858178	0.0
23	1.6819613014343765E-4	0.0	0.0	16.487257461180334	0.0
24	1.6819613014343765E-4	0.0	0.0	16.91817594660782	0.0
25	1.6819613014343765E-4	0.0	0.0	17.297121827820984	0.0
26	3.363922602868753E-4	0.0	0.0	17.66917166769827	0.0
27	3.363922602868753E-4	0.0	0.0	18.023056325520063	0.0
28	3.363922602868753E-4	0.0	0.0	18.401329422212655	0.0
29	3.363922602868753E-4	0.0	0.0	18.783639226028686	0.0
30	5.04588390430313E-4	0.0	0.0	19.185291584811218	0.0
31	5.04588390430313E-4	0.0	0.0	19.560368955031084	0.0
32	5.04588390430313E-4	0.0	0.0	19.953275115046154	0.0
33	0.001009176780860626	0.0	0.0	20.361318926774132	0.0
34	0.0011773729110040635	0.0	0.0	20.742955946069593	0.0
35	0.0011773729110040635	0.0	0.0	21.155541053311445	0.0
36	0.0011773729110040635	0.0	0.0	21.51682634085955	0.0
37	0.0011773729110040635	0.0	0.0	21.879793589709088	0.0
38	0.0011773729110040635	0.0	0.0	22.222072714550983	0.0
39	0.0011773729110040635	0.0	0.0	22.596813692510562	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAAC	25	3.888525E-5	45.000004	42
CGTTCTA	45	3.8380676E-10	45.000004	27
CGTTAGG	30	2.163726E-6	45.000004	2
ATAACGC	45	3.8380676E-10	45.000004	11
CACCGTA	30	2.163726E-6	45.000004	25
TCGACAA	30	2.163726E-6	45.000004	19
CGGGTTA	20	7.030536E-4	45.0	6
TTAGGCG	20	7.030536E-4	45.0	1
TTAGCGA	20	7.030536E-4	45.0	26
TTGTCGC	20	7.030536E-4	45.0	38
TGACGGA	20	7.030536E-4	45.0	34
TCGATCT	20	7.030536E-4	45.0	16
TAGCCGT	20	7.030536E-4	45.0	44
CCGTACA	20	7.030536E-4	45.0	28
CTACGCA	80	0.0	45.0	15
CCGAACA	20	7.030536E-4	45.0	11
GCCGTAC	20	7.030536E-4	45.0	43
TACGTAG	35	1.2106102E-7	45.0	1
GTACGAC	20	7.030536E-4	45.0	35
AGCACGT	20	7.030536E-4	45.0	34
>>END_MODULE