FastQCFastQC Report
Sat 18 Jun 2016
SRR3553892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752996
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC16620.22071830394849376No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG15970.212086119979389No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.1968137944955883No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC14470.19216569543530113TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.15365287465006455No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9030.11992095575540906No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC8920.11846012462217595No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC8580.1139448283921827TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT7800.103586207629257TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC207.0317334E-445.00000414
ATAGACG207.0317334E-445.0000041
TAACGTT207.0317334E-445.00000418
CGACATA207.0317334E-445.00000414
ATATGCG351.2111741E-745.0000041
TGGCACG207.0317334E-445.0000041
TACGCAT351.2111741E-745.00000416
TACGAAG302.164501E-645.0000041
CGAATGC302.164501E-645.00000445
CGCCGAT207.0317334E-445.00000410
TCGACAT207.0317334E-445.00000413
GCCGTAC406.8102963E-945.00000443
ACGGTAA207.0317334E-445.0000045
TAGACGA253.8895196E-545.018
GTGTACG453.8380676E-1045.01
TTGCGAT253.8895196E-545.028
CGTTAGG700.041.7857172
TTTACGG501.0804797E-940.52
CCGTACT451.9268555E-840.044
CACGACC2650.039.90566327
Produced by FastQC (version 0.11.5)