##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752996
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07879191921338	33.0	31.0	34.0	30.0	34.0
2	32.218135288899276	34.0	31.0	34.0	30.0	34.0
3	32.25319523609687	34.0	31.0	34.0	30.0	34.0
4	35.8020507413054	37.0	35.0	37.0	35.0	37.0
5	35.71241945508343	37.0	35.0	37.0	33.0	37.0
6	35.67230768822145	37.0	35.0	37.0	33.0	37.0
7	35.83320362923575	37.0	35.0	37.0	35.0	37.0
8	35.717497835313864	37.0	35.0	37.0	35.0	37.0
9	37.52944105944786	39.0	37.0	39.0	35.0	39.0
10	37.03669607806682	39.0	37.0	39.0	33.0	39.0
11	36.90295167570611	39.0	37.0	39.0	33.0	39.0
12	36.7243358530457	39.0	35.0	39.0	33.0	39.0
13	36.62374434923957	39.0	35.0	39.0	33.0	39.0
14	37.634543875399075	40.0	36.0	41.0	33.0	41.0
15	37.75717932100569	40.0	36.0	41.0	33.0	41.0
16	37.8818466499158	40.0	36.0	41.0	33.0	41.0
17	37.82678659647594	40.0	36.0	41.0	33.0	41.0
18	37.784142279640264	40.0	36.0	41.0	33.0	41.0
19	37.75947415391317	40.0	36.0	41.0	33.0	41.0
20	37.653069870225075	40.0	36.0	41.0	33.0	41.0
21	37.56703355661916	39.0	35.0	41.0	33.0	41.0
22	37.54976122051113	39.0	35.0	41.0	33.0	41.0
23	37.47107421553368	39.0	35.0	41.0	33.0	41.0
24	37.43346711005105	39.0	35.0	41.0	32.0	41.0
25	37.34620237026491	39.0	35.0	41.0	32.0	41.0
26	37.24984594871686	39.0	35.0	41.0	32.0	41.0
27	37.19316968483232	39.0	35.0	41.0	32.0	41.0
28	37.07062720120691	39.0	35.0	41.0	32.0	41.0
29	36.96723222965328	39.0	35.0	41.0	31.0	41.0
30	36.84767648168118	39.0	35.0	41.0	31.0	41.0
31	36.74658962331805	39.0	35.0	41.0	31.0	41.0
32	36.59366185212139	39.0	35.0	41.0	31.0	41.0
33	36.491231029115696	39.0	35.0	41.0	30.0	41.0
34	36.32880387146811	39.0	35.0	41.0	30.0	41.0
35	36.147432390079096	39.0	35.0	41.0	29.0	41.0
36	36.07349707036956	38.0	35.0	40.0	29.0	41.0
37	35.95981651960967	38.0	35.0	40.0	29.0	41.0
38	35.84520634903771	38.0	35.0	40.0	29.0	41.0
39	35.76857645990151	38.0	35.0	40.0	28.0	41.0
40	35.62911489569666	38.0	35.0	40.0	28.0	41.0
41	35.49337579482494	38.0	34.0	40.0	27.0	41.0
42	35.38331146513394	38.0	34.0	40.0	27.0	41.0
43	35.316637804184886	38.0	34.0	40.0	27.0	41.0
44	35.18477256187284	38.0	34.0	40.0	26.0	41.0
45	35.10059814394764	37.0	34.0	40.0	26.0	41.0
46	34.98856700434	37.0	34.0	40.0	26.0	41.0
47	34.842353744243	37.0	34.0	40.0	26.0	41.0
48	34.671703435343616	37.0	34.0	40.0	24.0	41.0
49	34.63066603275449	37.0	34.0	40.0	25.0	41.0
50	34.54208123283524	36.0	33.0	40.0	24.0	41.0
51	33.090433415316944	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	8.0
12	13.0
13	26.0
14	38.0
15	68.0
16	108.0
17	207.0
18	351.0
19	661.0
20	1152.0
21	1741.0
22	2538.0
23	3543.0
24	4845.0
25	6290.0
26	7723.0
27	9496.0
28	11125.0
29	13384.0
30	15948.0
31	19354.0
32	24950.0
33	34297.0
34	56372.0
35	62916.0
36	64319.0
37	90896.0
38	140433.0
39	180135.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55295911266461	19.233302700147146	24.33890751079687	11.874830676391374
2	32.046119766904475	24.15231953423391	25.10717188404719	18.694388814814424
3	32.11902852073583	25.370918305010914	25.546350843829185	16.963702330424066
4	27.965088791972338	25.915144303555397	26.496023883261	19.62374302121127
5	24.297074619254285	30.20507413053987	25.08977471327869	20.408076536927155
6	24.84209743478053	35.44295056016234	24.652720598781404	15.06223140627573
7	81.50308899383263	6.143193323736115	8.057413319592667	4.296304362838581
8	82.97879404405867	5.6396049912615736	7.010927016876583	4.3706739478031755
9	77.87836854379042	6.770819499705177	8.708811202184341	6.642000754320076
10	43.36450658436433	24.19083235501915	16.028770405154873	16.41589065546165
11	32.54373197201579	25.160293016164758	21.624948870910337	20.671026140909117
12	28.57996589623318	21.404628975452724	28.97903840126641	21.036366727047685
13	26.501203193642464	22.812073370907683	30.0296415917216	20.657081843728253
14	22.179267884557156	25.975835196999718	29.340793310987046	22.504103607456084
15	20.848583525012085	23.939835005763644	33.1964578829104	22.015123586313873
16	25.0108898320841	22.54845975277425	29.96656024733199	22.474090167809656
17	24.976759504698563	22.4054311045477	27.70944334365654	24.908366047097196
18	25.775834134577075	23.460549591232887	27.663493564374843	23.100122709815192
19	25.764545894002094	26.015277637597013	24.58658478929503	23.633591679105866
20	26.800806378785545	25.65803802410637	25.417000887122903	22.12415470998518
21	25.71049514207247	26.117004605602155	26.84303767881901	21.329462573506365
22	24.036648269047912	23.056563381478785	26.846888960897537	26.05989938857577
23	24.148999463476564	25.767998767589734	26.724843159857425	23.358158609076277
24	24.246343938082006	25.59641751084999	26.643833433378134	23.513405117689867
25	25.31792997572364	25.20796923224028	25.038512820785236	24.435587971250843
26	22.62362615472061	25.043028117015233	26.266009381191928	26.067336347072228
27	23.637044552693506	24.034789029423795	26.9196649118986	25.408501505984095
28	21.25801465080824	26.078093376326034	27.252867213106047	25.41102475975968
29	23.229339863691177	26.687525564544835	25.745687892100356	24.33744667966364
30	24.567328378902413	24.26666277111698	26.960966592120013	24.205042257860597
31	25.263082406812252	24.648072499721113	24.495614850543696	25.59323024292294
32	25.945556151692706	24.8711812546149	24.763478159246528	24.41978443444587
33	24.92948169711393	24.17396639557182	24.086183724747542	26.81036818256671
34	22.503439593304613	25.328554202147156	26.531615041779773	25.63639116276846
35	22.240622792152948	25.497745007941607	26.482610797401314	25.77902140250413
36	23.965333149180076	28.088728226975974	24.07595790681491	23.86998071702904
37	22.57754357260862	26.79589267406467	24.84581591402876	25.780747839297952
38	23.151783010799527	26.994964116675252	25.48725358434839	24.36599928817683
39	23.92031298971044	24.448204240128764	24.927356851829227	26.704125918331574
40	23.672104499891102	23.690165684811074	28.068409393941003	24.569320421356817
41	20.722819244723745	25.515806192861586	26.566940594637956	27.19443396777672
42	21.57049971048983	25.692832365643376	26.85764599015134	25.87902193371545
43	22.7876376501336	24.060287172840226	26.20598250189908	26.946092675127094
44	23.147666123060414	23.75032536693422	26.20465447359614	26.897354036409222
45	23.067586016393182	23.6875096282052	25.710362339242177	27.534542016159445
46	24.038773114332614	24.87901662160224	26.301467736880408	24.780742527184742
47	22.145004754341326	25.064143767031965	28.060308421293072	24.730543057333637
48	22.53969476597485	25.249270912461686	26.899478881693927	25.311555439869533
49	23.103973991893714	24.81261520645528	27.13693034225945	24.94648045939155
50	22.01578760046534	24.12443093987219	28.213695690282552	25.646085769379916
51	21.70463056908669	24.371444204218882	26.250869858538422	27.673055368156007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1371.0
1	1540.0
2	1709.0
3	2445.0
4	3181.0
5	2304.5
6	1428.0
7	1471.0
8	1514.0
9	1606.0
10	1698.0
11	1751.0
12	1804.0
13	1808.0
14	1812.0
15	1824.5
16	1837.0
17	1855.0
18	1873.0
19	1891.0
20	1909.0
21	2063.0
22	2217.0
23	2463.5
24	2710.0
25	3070.0
26	4119.0
27	4808.0
28	6205.5
29	7603.0
30	8795.0
31	9987.0
32	11322.0
33	12657.0
34	14106.0
35	15555.0
36	17090.0
37	18625.0
38	19577.5
39	20530.0
40	22354.0
41	24178.0
42	26992.5
43	29807.0
44	34455.5
45	39104.0
46	47149.0
47	55194.0
48	58114.0
49	61034.0
50	61698.5
51	62363.0
52	58192.5
53	54022.0
54	50211.5
55	46401.0
56	45807.5
57	45214.0
58	44659.5
59	44105.0
60	41936.5
61	39768.0
62	36722.5
63	33677.0
64	30678.0
65	27679.0
66	24268.0
67	20857.0
68	18568.0
69	16279.0
70	13743.5
71	11208.0
72	10000.5
73	8793.0
74	7195.0
75	4748.5
76	3900.0
77	3255.0
78	2610.0
79	2025.5
80	1441.0
81	1048.0
82	655.0
83	554.5
84	454.0
85	338.0
86	222.0
87	142.0
88	62.0
89	50.5
90	39.0
91	32.0
92	25.0
93	22.5
94	20.0
95	18.0
96	16.0
97	15.0
98	14.0
99	7.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	752996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.267350415135848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78412987193013	20.391650747089475
2	8.655445928154661	4.720221542445078
3	3.3131292980010656	2.7102077261774435
4	1.8129092365176407	1.9773292569185166
5	1.165284953498714	1.5887116580267362
6	0.8358036586783835	1.367409074366165
7	0.6778392757292261	1.293801673951549
8	0.5552810909077244	1.2112835267743862
9	0.5014009873067852	1.2304688777450274
>10	6.782640257018231	44.55601557059366
>50	0.8533931540773905	14.510793443367973
>100	0.05538967628392182	2.49551912072813
>500	0.004901741264063878	0.9648734686163767
>1k	0.002450870632031939	0.9817143131995134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	1662	0.22071830394849376	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	1597	0.212086119979389	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1482	0.1968137944955883	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1447	0.19216569543530113	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1157	0.15365287465006455	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	903	0.11992095575540906	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	892	0.11846012462217595	No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	858	0.1139448283921827	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	780	0.103586207629257	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9840849088175766E-4	0.0	0.0	0.10757029253807457	0.0
2	3.9840849088175766E-4	0.0	0.0	0.5569750702526972	0.0
3	3.9840849088175766E-4	0.0	0.0	0.7306811722771436	0.0
4	3.9840849088175766E-4	0.0	0.0	1.0429006262981477	0.0
5	3.9840849088175766E-4	0.0	0.0	2.089254126183937	0.0
6	3.9840849088175766E-4	0.0	0.0	2.7021391879903747	0.0
7	3.9840849088175766E-4	0.0	0.0	3.3206019686691564	0.0
8	3.9840849088175766E-4	0.0	0.0	4.205068818426659	0.0
9	3.9840849088175766E-4	0.0	0.0	4.591923463072845	0.0
10	3.9840849088175766E-4	0.0	0.0	5.588608704428709	0.0
11	3.9840849088175766E-4	0.0	0.0	6.545984308017572	0.0
12	3.9840849088175766E-4	0.0	0.0	7.3876886464204325	0.0
13	3.9840849088175766E-4	0.0	0.0	7.691010310811744	0.0
14	3.9840849088175766E-4	0.0	0.0	7.820360267518021	0.0
15	3.9840849088175766E-4	0.0	0.0	8.029790330891531	0.0
16	3.9840849088175766E-4	0.0	0.0	8.446658415184144	0.0
17	3.9840849088175766E-4	0.0	0.0	8.90124250328023	0.0
18	3.9840849088175766E-4	0.0	0.0	9.481458068834362	0.0
19	3.9840849088175766E-4	0.0	0.0	9.796333579461246	0.0
20	3.9840849088175766E-4	0.0	0.0	10.108951441973131	0.0
21	3.9840849088175766E-4	0.0	0.0	10.467917492257595	0.0
22	3.9840849088175766E-4	0.0	0.0	10.873762941635812	0.0
23	3.9840849088175766E-4	0.0	0.0	11.2562350928823	0.0
24	3.9840849088175766E-4	0.0	0.0	11.549862150662156	0.0
25	3.9840849088175766E-4	0.0	0.0	11.827951277297622	0.0
26	3.9840849088175766E-4	0.0	0.0	12.110954108653964	0.0
27	3.9840849088175766E-4	0.0	0.0	12.441633156085823	0.0
28	3.9840849088175766E-4	0.0	0.0	12.73871308745332	0.0
29	3.9840849088175766E-4	0.0	0.0	13.045354822601979	0.0
30	3.9840849088175766E-4	0.0	0.0	13.401133604959389	0.0
31	3.9840849088175766E-4	0.0	0.0	13.688386126885137	0.0
32	3.9840849088175766E-4	0.0	0.0	13.970326535599126	0.0
33	3.9840849088175766E-4	0.0	0.0	14.277897890559844	0.0
34	5.31211321175677E-4	0.0	0.0	14.579625920987628	0.0
35	5.31211321175677E-4	0.0	0.0	14.907516108983314	0.0
36	5.31211321175677E-4	0.0	0.0	15.196760673363471	0.0
37	6.640141514695961E-4	0.0	0.0	15.491848562276559	0.0
38	6.640141514695961E-4	0.0	0.0	15.811637777624316	0.0
39	6.640141514695961E-4	0.0	0.0	16.317616561044147	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAC	20	7.0317334E-4	45.000004	14
ATAGACG	20	7.0317334E-4	45.000004	1
TAACGTT	20	7.0317334E-4	45.000004	18
CGACATA	20	7.0317334E-4	45.000004	14
ATATGCG	35	1.2111741E-7	45.000004	1
TGGCACG	20	7.0317334E-4	45.000004	1
TACGCAT	35	1.2111741E-7	45.000004	16
TACGAAG	30	2.164501E-6	45.000004	1
CGAATGC	30	2.164501E-6	45.000004	45
CGCCGAT	20	7.0317334E-4	45.000004	10
TCGACAT	20	7.0317334E-4	45.000004	13
GCCGTAC	40	6.8102963E-9	45.000004	43
ACGGTAA	20	7.0317334E-4	45.000004	5
TAGACGA	25	3.8895196E-5	45.0	18
GTGTACG	45	3.8380676E-10	45.0	1
TTGCGAT	25	3.8895196E-5	45.0	28
CGTTAGG	70	0.0	41.785717	2
TTTACGG	50	1.0804797E-9	40.5	2
CCGTACT	45	1.9268555E-8	40.0	44
CACGACC	265	0.0	39.905663	27
>>END_MODULE