FastQCFastQC Report
Sat 18 Jun 2016
SRR3553891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958062
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.2554114451883072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13130.13704749796986No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10830.1130407009149721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG302.1651213E-645.0000041
ATTACGT207.0326985E-445.011
GTATACG207.0326985E-445.01
CACGTTA207.0326985E-445.037
TAGTGCG253.8903177E-545.01
AATACCG253.8903177E-545.01
CACGACC4050.041.66666427
CGTAAGG1050.040.7142872
CGAATAT1500.040.50000414
GCGATCA501.0804797E-940.59
TAATGCG451.927765E-840.01
TTGCGAG800.039.3751
GCGTAAG800.039.3751
AAATGCG403.457426E-739.3751
AGACACG4300.038.72093224
AACACGT4300.038.72093241
TATACGG356.2472027E-638.5714262
CGAGACA4400.038.35227222
ACGACCA4400.038.35227228
TTAGCGG2700.038.3333362
Produced by FastQC (version 0.11.5)