##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958062
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20973590435692	33.0	31.0	34.0	30.0	34.0
2	32.35517012468922	34.0	31.0	34.0	30.0	34.0
3	32.3856326626043	34.0	31.0	34.0	30.0	34.0
4	35.90657598360023	37.0	35.0	37.0	35.0	37.0
5	35.8264486014475	37.0	35.0	37.0	35.0	37.0
6	35.79491515162902	37.0	35.0	37.0	35.0	37.0
7	35.96151919186858	37.0	35.0	37.0	35.0	37.0
8	35.888163814032914	37.0	35.0	37.0	35.0	37.0
9	37.67893727128307	39.0	38.0	39.0	35.0	39.0
10	37.158180785794656	39.0	37.0	39.0	34.0	39.0
11	37.05205404243149	39.0	37.0	39.0	33.0	39.0
12	36.890703315651805	39.0	37.0	39.0	33.0	39.0
13	36.82648930862512	39.0	37.0	39.0	33.0	39.0
14	37.86500247374387	40.0	37.0	41.0	33.0	41.0
15	38.007738538841956	40.0	37.0	41.0	33.0	41.0
16	38.10149760662671	40.0	37.0	41.0	33.0	41.0
17	38.072579853913425	40.0	37.0	41.0	33.0	41.0
18	38.02704522254301	40.0	37.0	41.0	33.0	41.0
19	37.9713609348873	40.0	37.0	41.0	33.0	41.0
20	37.87370754711073	40.0	36.0	41.0	33.0	41.0
21	37.77956541434688	40.0	36.0	41.0	33.0	41.0
22	37.77086555984894	40.0	36.0	41.0	33.0	41.0
23	37.69051376633245	40.0	36.0	41.0	33.0	41.0
24	37.63085270055591	40.0	35.0	41.0	33.0	41.0
25	37.54428627792356	40.0	35.0	41.0	33.0	41.0
26	37.47486801480489	39.0	35.0	41.0	33.0	41.0
27	37.40213785746643	39.0	35.0	41.0	33.0	41.0
28	37.29894620598667	39.0	35.0	41.0	32.0	41.0
29	37.21251756149393	39.0	35.0	41.0	32.0	41.0
30	37.0545507493252	39.0	35.0	41.0	31.0	41.0
31	36.92149777363052	39.0	35.0	41.0	31.0	41.0
32	36.77810517482167	39.0	35.0	41.0	31.0	41.0
33	36.678810974655086	39.0	35.0	41.0	31.0	41.0
34	36.50759971692855	39.0	35.0	41.0	30.0	41.0
35	36.34574589118449	39.0	35.0	41.0	30.0	41.0
36	36.23561940667723	39.0	35.0	41.0	30.0	41.0
37	36.08073903359073	39.0	35.0	41.0	29.0	41.0
38	35.91919938375596	38.0	35.0	41.0	29.0	41.0
39	35.86147451835059	38.0	35.0	41.0	29.0	41.0
40	35.74384956297192	38.0	35.0	40.0	28.0	41.0
41	35.60068868194334	38.0	35.0	40.0	27.0	41.0
42	35.47542434623229	38.0	34.0	40.0	27.0	41.0
43	35.41846456701132	38.0	34.0	40.0	27.0	41.0
44	35.257987478889675	38.0	34.0	40.0	26.0	41.0
45	35.20140763332645	38.0	34.0	40.0	26.0	41.0
46	35.10902947825924	37.0	34.0	40.0	26.0	41.0
47	34.95504257553269	37.0	34.0	40.0	26.0	41.0
48	34.78458805380028	37.0	34.0	40.0	25.0	41.0
49	34.718564143030406	37.0	34.0	40.0	25.0	41.0
50	34.637667499598145	37.0	34.0	40.0	24.0	41.0
51	33.204974208349775	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	13.0
12	12.0
13	23.0
14	38.0
15	60.0
16	130.0
17	253.0
18	411.0
19	795.0
20	1293.0
21	1968.0
22	2830.0
23	4110.0
24	5688.0
25	7520.0
26	10037.0
27	12189.0
28	14344.0
29	16320.0
30	19172.0
31	23218.0
32	28868.0
33	40063.0
34	66153.0
35	77476.0
36	79431.0
37	112641.0
38	180416.0
39	252493.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66533689886458	19.776068772167147	25.914502401723478	10.644091927244792
2	32.98575666292996	23.763180253470026	25.224776684598698	18.026286399001318
3	33.60659331024506	23.697422505015332	26.218971214806558	16.477012969933053
4	28.127615958048647	26.749938939233576	25.301494057795843	19.820951044921937
5	25.724744327611365	30.801451263070657	24.412511925115492	19.061292484202482
6	25.432278913055733	36.19838799576645	23.778314973352455	14.59101811782536
7	83.50492974358653	5.780941108195503	6.9255434408211585	3.788585707396807
8	85.45146347522395	4.712325507117494	6.317753965818495	3.518457051840069
9	79.9591258185796	6.947775822441554	8.272846642492865	4.8202517164859895
10	42.30070705236196	26.18369166087372	15.608384426060109	15.907216860704212
11	33.05589826128163	25.754909389997728	21.92446835382261	19.264723994898034
12	28.004555028797718	23.89542639202891	28.336892601940168	19.763125977233205
13	25.89540134145807	24.99378954597928	28.76473547640967	20.346073636152983
14	22.25951973880605	27.03353227661675	29.583367255981347	21.123580728595854
15	22.114852692205726	24.821775626212084	31.660581465500144	21.40279021608205
16	25.94017923683436	24.159709914389673	28.938210679475855	20.961900169300108
17	25.590515018860994	23.51382269623469	27.46419334030574	23.431468944598574
18	26.95263980827963	23.27845170771829	28.238360356636626	21.530548127365453
19	26.815070423417275	26.112923798251053	25.304729756529326	21.76727602180235
20	27.03635046583624	26.029004385937444	25.941014255862356	20.99363089236396
21	26.298924286737186	26.287651529859236	26.601201174871775	20.81222300853181
22	25.41390849443982	23.688028540950377	26.942515202565176	23.955547762044628
23	24.405101131242027	26.431692312188567	27.003993478501393	22.159213078068017
24	23.692516768225854	26.726349651692686	28.001110575307237	21.580023004774223
25	25.6955186616315	26.161563656631827	25.96982241232822	22.173095269408453
26	23.22699366011803	26.409773062703668	25.5887406034265	24.7744926737518
27	23.62968158636915	24.964668257377916	28.362047550158547	23.04360260609439
28	22.024357505046645	26.91443768774881	27.884834175658778	23.17637063154577
29	23.89313008970192	27.09855938342195	25.703138210261965	23.305172316614165
30	25.483215073763493	25.328840930962716	26.825716916024223	22.362227079249568
31	26.711423686567258	25.575171544221565	25.36641678722254	22.34698798198864
32	25.495740359183433	26.451941523617467	25.639885518891266	22.412432598307834
33	25.253689218443064	26.120856479017018	24.431926117516404	24.193528185023517
34	23.423118754318615	26.49442311666677	27.268903265133154	22.81355486388146
35	24.18434297571556	27.274957153086127	25.757831956595712	22.782867914602605
36	25.797704115182523	28.59460034945546	24.272646237926146	21.335049297435866
37	24.67011529525229	27.771793474743806	24.96352010621442	22.594571123789482
38	24.040510948143233	28.734674791401808	25.077708958292888	22.147105302162075
39	24.429629815189415	26.167304412449298	25.073742617909904	24.329323154451384
40	25.34888138763462	25.64677442587223	26.701820967745306	22.302523218747847
41	22.480277894332517	26.401214117666704	25.984644000075154	25.133863987925626
42	23.116249261530047	26.694514551250336	26.537844106122567	23.651392081097047
43	23.45818955349445	24.87385993808334	27.209095027252932	24.458855481169277
44	23.419569923449632	25.44636985915317	27.289987495590058	23.844072721807148
45	23.926843982957262	25.37132252401201	25.691865453384015	25.0099680396467
46	23.92726149247126	25.651680162661705	26.733134181295153	23.68792416357188
47	23.122198772104518	26.77488513269496	27.600614574004606	22.502301521195914
48	22.47725095035603	27.65102884781987	26.508931572278204	23.362788629545896
49	23.7900052397444	27.584331702958682	26.233270915660988	22.392392141635927
50	22.98076742423768	26.275021867060794	27.01672751867833	23.727483190023193
51	23.060824873546807	25.774323582398633	26.039546501165894	25.125305042888662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1623.0
1	1948.5
2	2274.0
3	3633.0
4	4992.0
5	3596.5
6	2201.0
7	2240.5
8	2280.0
9	2374.0
10	2468.0
11	2497.0
12	2526.0
13	2505.0
14	2484.0
15	2448.5
16	2413.0
17	2407.5
18	2402.0
19	2765.5
20	3129.0
21	3229.0
22	3329.0
23	3936.0
24	4543.0
25	5255.0
26	7104.5
27	8242.0
28	9927.5
29	11613.0
30	14276.5
31	16940.0
32	17959.5
33	18979.0
34	21996.5
35	25014.0
36	26819.0
37	28624.0
38	30552.5
39	32481.0
40	35913.0
41	39345.0
42	42065.0
43	44785.0
44	47932.0
45	51079.0
46	54906.0
47	58733.0
48	64082.0
49	69431.0
50	68835.5
51	68240.0
52	67235.5
53	66231.0
54	62702.5
55	59174.0
56	57180.0
57	55186.0
58	53593.0
59	52000.0
60	49855.5
61	47711.0
62	43536.5
63	39362.0
64	35080.5
65	30799.0
66	28100.5
67	25402.0
68	21872.0
69	18342.0
70	16513.0
71	14684.0
72	13148.5
73	11613.0
74	10155.0
75	7303.5
76	5910.0
77	4578.0
78	3246.0
79	2506.0
80	1766.0
81	1339.5
82	913.0
83	703.0
84	493.0
85	341.0
86	189.0
87	143.0
88	97.0
89	65.5
90	34.0
91	39.5
92	45.0
93	31.5
94	18.0
95	14.5
96	11.0
97	6.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	958062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.18386778715352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19329053818564	18.18464596856503
2	8.270637444025207	4.0003200492357385
3	2.994833214197053	2.1727995149015262
4	1.6161382858788913	1.563378985258081
5	1.058407476575458	1.2798193239217837
6	0.8071575876352393	1.1712115429661047
7	0.6210704864825881	1.0513920572118622
8	0.5310911354106107	1.0275070241359567
9	0.47826260727563563	1.0409615695703969
>10	6.88862462518212	41.2604614623917
>50	1.4480834323047687	22.564670784609813
>100	0.08676353694531999	3.426487820875923
>500	0.0043381768472659996	0.7482459013621274
>1k	0.0013014530541798	0.5080979949939404
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2447	0.2554114451883072	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1313	0.13704749796986	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1083	0.1130407009149721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.087547569990251E-4	0.0	0.0	0.041542196642806	0.0
2	2.087547569990251E-4	0.0	0.0	0.19153248954660554	0.0
3	2.087547569990251E-4	0.0	0.0	0.265744805659759	0.0
4	2.087547569990251E-4	0.0	0.0	0.37951614822422763	0.0
5	2.087547569990251E-4	0.0	0.0	0.7371130469635577	0.0
6	2.087547569990251E-4	0.0	0.0	1.0063023061138006	0.0
7	2.087547569990251E-4	0.0	0.0	1.2652625821710912	0.0
8	2.087547569990251E-4	0.0	0.0	1.6896610031501094	0.0
9	2.087547569990251E-4	0.0	0.0	1.8943450423876533	0.0
10	2.087547569990251E-4	0.0	0.0	2.3150902551191885	0.0
11	2.087547569990251E-4	0.0	0.0	2.8877045535675143	0.0
12	2.087547569990251E-4	0.0	0.0	3.3011433498040836	0.0
13	2.087547569990251E-4	0.0	0.0	3.4737835338422776	0.0
14	2.087547569990251E-4	0.0	0.0	3.5510227939319168	0.0
15	2.087547569990251E-4	0.0	0.0	3.679615724243316	0.0
16	2.087547569990251E-4	0.0	0.0	3.9493268702860567	2.087547569990251E-4
17	2.087547569990251E-4	0.0	0.0	4.267991006845069	2.087547569990251E-4
18	2.087547569990251E-4	0.0	0.0	4.635608133920352	2.087547569990251E-4
19	2.087547569990251E-4	0.0	0.0	4.8361170780179155	2.087547569990251E-4
20	2.087547569990251E-4	0.0	0.0	5.056457724030387	2.087547569990251E-4
21	2.087547569990251E-4	0.0	0.0	5.3141654715456825	2.087547569990251E-4
22	2.087547569990251E-4	0.0	0.0	5.59535812922337	2.087547569990251E-4
23	2.087547569990251E-4	0.0	0.0	5.878638334471047	2.087547569990251E-4
24	2.087547569990251E-4	0.0	0.0	6.116305625314437	2.087547569990251E-4
25	3.1313213549853767E-4	0.0	0.0	6.324225363285466	2.087547569990251E-4
26	3.1313213549853767E-4	0.0	0.0	6.52254238243454	2.087547569990251E-4
27	4.175095139980502E-4	0.0	0.0	6.7658460517169035	2.087547569990251E-4
28	4.175095139980502E-4	0.0	0.0	6.99140556665435	2.087547569990251E-4
29	4.175095139980502E-4	0.0	0.0	7.240554369132687	2.087547569990251E-4
30	4.175095139980502E-4	0.0	0.0	7.5303059718473335	2.087547569990251E-4
31	4.175095139980502E-4	0.0	0.0	7.75576110940628	2.087547569990251E-4
32	4.175095139980502E-4	0.0	0.0	7.994889683548664	2.087547569990251E-4
33	4.175095139980502E-4	0.0	0.0	8.226294331682084	2.087547569990251E-4
34	4.175095139980502E-4	0.0	0.0	8.494544194425831	2.087547569990251E-4
35	4.175095139980502E-4	0.0	0.0	8.789827798200951	2.087547569990251E-4
36	4.175095139980502E-4	0.0	0.0	9.028225730693839	2.087547569990251E-4
37	5.218868924975628E-4	0.0	0.0	9.283115288989649	2.087547569990251E-4
38	5.218868924975628E-4	0.0	0.0	9.570048702484808	2.087547569990251E-4
39	6.262642709970753E-4	0.0	0.0	10.079619064319429	2.087547569990251E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	30	2.1651213E-6	45.000004	1
ATTACGT	20	7.0326985E-4	45.0	11
GTATACG	20	7.0326985E-4	45.0	1
CACGTTA	20	7.0326985E-4	45.0	37
TAGTGCG	25	3.8903177E-5	45.0	1
AATACCG	25	3.8903177E-5	45.0	1
CACGACC	405	0.0	41.666664	27
CGTAAGG	105	0.0	40.714287	2
CGAATAT	150	0.0	40.500004	14
GCGATCA	50	1.0804797E-9	40.5	9
TAATGCG	45	1.927765E-8	40.0	1
TTGCGAG	80	0.0	39.375	1
GCGTAAG	80	0.0	39.375	1
AAATGCG	40	3.457426E-7	39.375	1
AGACACG	430	0.0	38.720932	24
AACACGT	430	0.0	38.720932	41
TATACGG	35	6.2472027E-6	38.571426	2
CGAGACA	440	0.0	38.352272	22
ACGACCA	440	0.0	38.352272	28
TTAGCGG	270	0.0	38.333336	2
>>END_MODULE