FastQCFastQC Report
Sat 18 Jun 2016
SRR3553886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664183
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17670.266041136253111No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.2372840015477662No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT10080.15176540200517027No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8790.13234304401046096No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7790.11728695254169408No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7380.11111395503949967No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC7260.10930722406324762No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6890.10373647021980387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA351.210883E-745.00000442
ATTTCGT302.1641117E-645.00000438
TCGTTTA502.1827873E-1145.038
TCGTTAG453.8380676E-1045.01
GATCGGA207.031134E-445.03
ACTCTCG253.8890215E-545.029
TCGATTA406.8084773E-945.041
TACATTA207.031134E-445.028
CGTAGGT253.8890215E-545.023
CTTGCGT207.031134E-445.023
GTATACG207.031134E-445.01
TATCGTC207.031134E-445.035
TAATGCG207.031134E-445.01
TACACCG253.8890215E-545.020
AGTCGTA253.8890215E-545.020
ATAGCGG1350.043.3333322
CGTAAGG650.041.538462
ACACGTG2300.041.0869642
AACACGT2300.041.0869641
CGCATCG556.002665E-1140.90909221