##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664183
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.189387563367326	33.0	31.0	34.0	30.0	34.0
2	32.34595134172359	34.0	31.0	34.0	30.0	34.0
3	32.37101521719165	34.0	31.0	34.0	30.0	34.0
4	35.891513332921804	37.0	35.0	37.0	35.0	37.0
5	35.798406463278944	37.0	35.0	37.0	35.0	37.0
6	35.773396789740175	37.0	35.0	37.0	35.0	37.0
7	35.902564504059875	37.0	35.0	37.0	35.0	37.0
8	35.790107545661364	37.0	35.0	37.0	35.0	37.0
9	37.5950272741097	39.0	37.0	39.0	35.0	39.0
10	37.09655772580749	39.0	37.0	39.0	33.0	39.0
11	37.02587841001652	39.0	37.0	39.0	33.0	39.0
12	36.92123706869944	39.0	37.0	39.0	33.0	39.0
13	36.85926017377741	39.0	37.0	39.0	33.0	39.0
14	38.00837720929322	40.0	37.0	41.0	33.0	41.0
15	38.11598008380221	40.0	37.0	41.0	33.0	41.0
16	38.19492971063698	40.0	37.0	41.0	33.0	41.0
17	38.132460481523914	40.0	37.0	41.0	33.0	41.0
18	38.083785643414544	40.0	37.0	41.0	33.0	41.0
19	38.02778601680561	40.0	37.0	41.0	34.0	41.0
20	37.92530673022345	40.0	36.0	41.0	33.0	41.0
21	37.807750273644466	40.0	36.0	41.0	33.0	41.0
22	37.77787898817043	40.0	36.0	41.0	33.0	41.0
23	37.69362208909291	40.0	36.0	41.0	33.0	41.0
24	37.63360098045268	40.0	36.0	41.0	33.0	41.0
25	37.540564874439724	40.0	35.0	41.0	33.0	41.0
26	37.462958250963965	40.0	35.0	41.0	33.0	41.0
27	37.40328192681836	39.0	35.0	41.0	33.0	41.0
28	37.274269290240795	39.0	35.0	41.0	32.0	41.0
29	37.18984376293883	39.0	35.0	41.0	32.0	41.0
30	37.06194527712995	39.0	35.0	41.0	31.0	41.0
31	36.95521866714444	39.0	35.0	41.0	31.0	41.0
32	36.803638455064345	39.0	35.0	41.0	31.0	41.0
33	36.676205202481846	39.0	35.0	41.0	31.0	41.0
34	36.559880334185	39.0	35.0	41.0	30.0	41.0
35	36.39571624085531	39.0	35.0	41.0	30.0	41.0
36	36.261640240716794	39.0	35.0	41.0	30.0	41.0
37	36.14351466388029	39.0	35.0	41.0	30.0	41.0
38	36.008372692465784	39.0	35.0	41.0	29.0	41.0
39	35.94609015888693	38.0	35.0	41.0	29.0	41.0
40	35.79958234402265	38.0	35.0	40.0	28.0	41.0
41	35.67073833566954	38.0	35.0	40.0	28.0	41.0
42	35.54342101499135	38.0	35.0	40.0	27.0	41.0
43	35.418670757908586	38.0	34.0	40.0	27.0	41.0
44	35.294808810222484	38.0	34.0	40.0	27.0	41.0
45	35.18772235965088	38.0	34.0	40.0	26.0	41.0
46	35.06768917602528	37.0	34.0	40.0	26.0	41.0
47	34.97106068658788	37.0	34.0	40.0	26.0	41.0
48	34.79332804362653	37.0	34.0	40.0	25.0	41.0
49	34.70419598213143	37.0	34.0	40.0	25.0	41.0
50	34.61981110627643	37.0	34.0	40.0	25.0	41.0
51	33.232944835986466	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	9.0
12	4.0
13	15.0
14	26.0
15	38.0
16	83.0
17	157.0
18	286.0
19	584.0
20	977.0
21	1475.0
22	2155.0
23	3067.0
24	4039.0
25	5310.0
26	6749.0
27	7927.0
28	9283.0
29	10862.0
30	12874.0
31	16065.0
32	20307.0
33	26605.0
34	44675.0
35	54874.0
36	55866.0
37	79841.0
38	125638.0
39	174326.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64005552686534	21.24309113602727	23.932109072349036	12.184744264758358
2	31.0948940277002	25.666269687721606	24.700120298170837	18.53871598640736
3	32.85464999856968	24.830506050290357	26.24788650116007	16.0669574499799
4	29.04094202953102	26.44512129940092	25.453978798011995	19.05995787305607
5	24.222842198610923	31.735982402440293	24.407429880018007	19.633745518930777
6	24.717735925189295	37.29393856813559	23.451066949921934	14.537258556753185
7	82.6949801485434	6.597579281613651	6.927307684779646	3.780132885063303
8	84.47476072106633	5.734564118623934	6.2434600102682545	3.5472151500414792
9	79.23238023255638	7.715343512254906	8.357335252483127	4.69494100270558
10	40.907852203383705	27.124301585556992	16.13019303414872	15.83765317691058
11	31.36334413858831	27.52027679118556	21.188437523995646	19.927941546230482
12	27.123699341898245	24.331848300844797	28.074792639980245	20.469659717276713
13	25.069747343729066	25.312903220949647	28.48326440152789	21.134085033793397
14	21.78766996445257	28.832866845432658	27.93221145377102	21.44725173634375
15	21.676104326669005	26.382036276146785	30.177074691764165	21.76478470542004
16	23.574828021795195	24.089595789112337	30.043677721350893	22.29189846774157
17	24.217572566596857	23.96568415632439	26.93911166049116	24.877631616587596
18	25.10287074496035	25.151803042233844	26.45460663702624	23.290719575779566
19	25.785062249410178	26.95190933823961	24.740169501477755	22.522858910872458
20	26.84591445429949	25.990126215214783	25.338348015531864	21.82561131495386
21	26.43021576884684	25.596710545135902	26.396791245786176	21.576282440231083
22	25.00289829760774	24.66293175224298	24.849777847370376	25.4843921027789
23	23.613070494125864	27.044052618028463	25.369213003042834	23.973663884802832
24	22.890980347283808	27.038331303270336	27.156822743129528	22.91386560631633
25	24.56551884044006	26.96816991702588	25.426727272453526	23.039583970080535
26	23.340103555797125	27.210121306928965	25.044151988232162	24.405623149041755
27	23.998054752982235	26.259630252505712	26.18344642967375	23.558868564838306
28	22.06154026826944	27.15622049947078	28.19162790977788	22.59061132248191
29	23.859990394213643	26.46574814471313	26.62654720159956	23.04771425947367
30	23.850505056588318	25.882625722127788	27.441081750059848	22.825787471224046
31	25.35731869078251	27.028544843815634	23.957252745101876	23.65688372029998
32	24.821020712665035	27.30527580501157	24.700120298170837	23.17358318415256
33	24.493550723219354	26.999486587280913	24.066559969165123	24.440402720334607
34	23.076923076923077	27.603386416093155	25.046862084696535	24.272828422287233
35	24.381985085435794	26.06224489335018	24.60677253106448	24.94899749014955
36	25.025030752066822	27.785565122865236	23.823705213773916	23.365698911294025
37	23.223719968743552	28.82850057890672	24.569583985136624	23.378195467213104
38	23.480878011030093	29.127213433647054	24.64621949071265	22.745689064610204
39	23.287557796571125	26.555633010781666	25.15270640772197	25.00410278492524
40	24.246179140387515	25.414832960193202	27.495885923006156	22.843101976413127
41	21.80242493409196	27.135443093243882	26.370141963886457	24.6919900087777
42	22.839488514460623	27.65472768800165	25.737485000368878	23.768298797168853
43	23.706869943976283	25.09308428550565	25.88879871962998	25.311247050888085
44	23.316616053105847	25.816529480579902	26.058480870482985	24.80837359583127
45	23.98284810059878	25.734925464819185	25.776329716358294	24.505896718223745
46	24.00016260578786	26.10078848751022	25.61568122038655	24.28336768631537
47	23.15340802158441	26.845011088811365	26.782076626471923	23.219504263132297
48	23.140459782921276	27.61934587305005	25.58918249940152	23.65101184462716
49	22.730632973141436	27.522836326735252	26.15649602594466	23.590034674178654
50	21.942898267495554	26.455058319770302	27.52870820240807	24.073335210326068
51	22.624336967371946	26.143096104537456	25.751788287264205	25.4807786408264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2027.0
1	2190.5
2	2354.0
3	2988.0
4	3622.0
5	2672.5
6	1723.0
7	1693.5
8	1664.0
9	1777.0
10	1890.0
11	2011.5
12	2133.0
13	2162.5
14	2192.0
15	2245.0
16	2298.0
17	2161.5
18	2025.0
19	2125.5
20	2226.0
21	2255.0
22	2284.0
23	2596.0
24	2908.0
25	3679.5
26	5182.0
27	5913.0
28	7329.0
29	8745.0
30	9393.5
31	10042.0
32	12021.0
33	14000.0
34	16077.5
35	18155.0
36	18908.5
37	19662.0
38	20789.5
39	21917.0
40	24075.5
41	26234.0
42	29346.0
43	32458.0
44	34601.0
45	36744.0
46	39548.5
47	42353.0
48	44418.5
49	46484.0
50	46485.5
51	46487.0
52	43509.5
53	40532.0
54	39068.5
55	37605.0
56	35955.5
57	34306.0
58	34179.5
59	34053.0
60	32823.0
61	31593.0
62	29937.5
63	28282.0
64	25786.5
65	23291.0
66	19795.0
67	16299.0
68	15114.5
69	13930.0
70	13136.5
71	12343.0
72	11218.0
73	10093.0
74	8956.5
75	6135.0
76	4450.0
77	3826.0
78	3202.0
79	2458.5
80	1715.0
81	1310.0
82	905.0
83	645.0
84	385.0
85	305.0
86	225.0
87	169.5
88	114.0
89	71.0
90	28.0
91	18.0
92	8.0
93	5.5
94	3.0
95	6.5
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	664183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.170755002679783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69868819280613	18.29694446332566
2	7.887872401045694	3.8131166259615004
3	2.705998059839635	1.9621804842633195
4	1.5392599570986794	1.488203012338703
5	1.0075848528687361	1.217704331155069
6	0.7535608537547535	1.0928480865429815
7	0.6576476224424689	1.1127087692106241
8	0.5625887930794757	1.0878556707821858
9	0.49370064466607627	1.0739805594199918
>10	7.311709525433765	44.539553589207046
>50	1.2913800336329317	19.70734348542243
>100	0.0837584339335977	3.2046094607469677
>500	0.004375440578620776	0.745591800895115
>1k	0.0018751888194089039	0.6573596607283982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1767	0.266041136253111	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1576	0.2372840015477662	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1008	0.15176540200517027	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	879	0.13234304401046096	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	779	0.11728695254169408	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	738	0.11111395503949967	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC	726	0.10930722406324762	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	689	0.10373647021980387	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0112182937533784E-4	0.0	0.0	0.05359968562881013	0.0
2	3.0112182937533784E-4	0.0	0.0	0.24180082898839628	0.0
3	3.0112182937533784E-4	0.0	0.0	0.33484747426537564	0.0
4	3.0112182937533784E-4	0.0	0.0	0.4455097465608123	0.0
5	3.0112182937533784E-4	0.0	0.0	0.8330535409668721	0.0
6	3.0112182937533784E-4	0.0	0.0	1.1352292967450235	0.0
7	3.0112182937533784E-4	0.0	0.0	1.420241108248781	0.0
8	3.0112182937533784E-4	0.0	0.0	1.8851732128043024	0.0
9	3.0112182937533784E-4	0.0	0.0	2.0929472750732856	0.0
10	3.0112182937533784E-4	0.0	0.0	2.541167118098476	0.0
11	3.0112182937533784E-4	0.0	0.0	3.147626482460406	0.0
12	3.0112182937533784E-4	0.0	0.0	3.5850059396280844	0.0
13	3.0112182937533784E-4	0.0	0.0	3.759355478836405	0.0
14	3.0112182937533784E-4	0.0	0.0	3.83072135239836	0.0
15	4.5168274406300675E-4	0.0	0.0	3.955084667930375	0.0
16	4.5168274406300675E-4	0.0	0.0	4.211489905643475	0.0
17	4.5168274406300675E-4	0.0	0.0	4.527517265572892	0.0
18	4.5168274406300675E-4	0.0	0.0	4.918825082846143	0.0
19	4.5168274406300675E-4	0.0	0.0	5.102659959679787	0.0
20	4.5168274406300675E-4	0.0	0.0	5.298389148773756	0.0
21	4.5168274406300675E-4	0.0	0.0	5.534318102089334	0.0
22	4.5168274406300675E-4	0.0	0.0	5.786808756020554	0.0
23	4.5168274406300675E-4	0.0	0.0	6.041557823672091	0.0
24	4.5168274406300675E-4	0.0	0.0	6.258064418992959	0.0
25	4.5168274406300675E-4	0.0	0.0	6.442200417655977	0.0
26	4.5168274406300675E-4	0.0	0.0	6.619410614243364	0.0
27	4.5168274406300675E-4	0.0	0.0	6.793157909792933	0.0
28	4.5168274406300675E-4	0.0	0.0	6.979552322176268	0.0
29	4.5168274406300675E-4	0.0	0.0	7.179798338710867	0.0
30	4.5168274406300675E-4	0.0	0.0	7.414974487453006	0.0
31	4.5168274406300675E-4	0.0	0.0	7.608896945570724	0.0
32	4.5168274406300675E-4	0.0	0.0	7.803120525517817	0.0
33	4.5168274406300675E-4	0.0	0.0	8.009539539554611	0.0
34	4.5168274406300675E-4	0.0	0.0	8.215506870847342	0.0
35	4.5168274406300675E-4	0.0	0.0	8.436379732694151	0.0
36	4.5168274406300675E-4	0.0	0.0	8.644756038621885	0.0
37	7.528045734383446E-4	0.0	0.0	8.84274364143617	0.0
38	7.528045734383446E-4	0.0	0.0	9.049162655472964	0.0
39	7.528045734383446E-4	0.0	0.0	9.260399618779765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTA	35	1.210883E-7	45.000004	42
ATTTCGT	30	2.1641117E-6	45.000004	38
TCGTTTA	50	2.1827873E-11	45.0	38
TCGTTAG	45	3.8380676E-10	45.0	1
GATCGGA	20	7.031134E-4	45.0	3
ACTCTCG	25	3.8890215E-5	45.0	29
TCGATTA	40	6.8084773E-9	45.0	41
TACATTA	20	7.031134E-4	45.0	28
CGTAGGT	25	3.8890215E-5	45.0	23
CTTGCGT	20	7.031134E-4	45.0	23
GTATACG	20	7.031134E-4	45.0	1
TATCGTC	20	7.031134E-4	45.0	35
TAATGCG	20	7.031134E-4	45.0	1
TACACCG	25	3.8890215E-5	45.0	20
AGTCGTA	25	3.8890215E-5	45.0	20
ATAGCGG	135	0.0	43.333332	2
CGTAAGG	65	0.0	41.53846	2
ACACGTG	230	0.0	41.08696	42
AACACGT	230	0.0	41.08696	41
CGCATCG	55	6.002665E-11	40.909092	21
>>END_MODULE