FastQCFastQC Report
Sat 18 Jun 2016
SRR3553884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538904
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.29170316048869555No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.22657096625744103No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC10760.19966450425307664No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8860.1644077609370129No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC7510.139356917001915No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6330.11746062378457016No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6220.11541944390837701No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5710.10595579175511778No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG207.029947E-445.00000421
ATTCGCG207.029947E-445.0000041
CCGTATT207.029947E-445.00000416
GTATGAT207.029947E-445.00000432
TGTTACG207.029947E-445.0000041
CATCCGA207.029947E-445.00000423
TAAGACG207.029947E-445.0000041
CTAACGG253.888039E-545.02
ATATACG253.888039E-545.01
TTAGTGC253.888039E-545.014
ACGCATT1550.043.54838617
CTACGCA1600.042.18750415
AACGGGA1350.041.6666644
TACGAAT650.041.5384612
TACGCAT1650.040.9090916
CGCTACA1650.040.9090928
TATGGGA2400.039.3750044
TGGGCGA3850.039.155846
CGATTTA356.242115E-638.57142610
CTACGAA700.038.57142611