##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538904
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2047952882146	33.0	31.0	34.0	30.0	34.0
2	32.35990640262459	34.0	31.0	34.0	30.0	34.0
3	32.373161082493354	34.0	31.0	34.0	30.0	34.0
4	35.89470480827754	37.0	35.0	37.0	35.0	37.0
5	35.825889212178794	37.0	35.0	37.0	35.0	37.0
6	35.80404673188546	37.0	35.0	37.0	35.0	37.0
7	35.933832370886094	37.0	35.0	37.0	35.0	37.0
8	35.82035947033238	37.0	35.0	37.0	35.0	37.0
9	37.647709053931685	39.0	38.0	39.0	35.0	39.0
10	37.18027886228345	39.0	37.0	39.0	34.0	39.0
11	37.0857666671615	39.0	37.0	39.0	33.0	39.0
12	36.93850481718451	39.0	37.0	39.0	33.0	39.0
13	36.878627733325416	39.0	37.0	39.0	33.0	39.0
14	38.034818817451715	40.0	37.0	41.0	33.0	41.0
15	38.12722674168312	40.0	37.0	41.0	33.0	41.0
16	38.19712045188011	40.0	37.0	41.0	34.0	41.0
17	38.15337982275136	40.0	37.0	41.0	33.0	41.0
18	38.096879963778335	40.0	37.0	41.0	33.0	41.0
19	37.9957858913647	40.0	36.0	41.0	34.0	41.0
20	37.923754509151905	40.0	36.0	41.0	33.0	41.0
21	37.816327212267865	40.0	36.0	41.0	33.0	41.0
22	37.755158618232564	40.0	36.0	41.0	33.0	41.0
23	37.673060507994	40.0	35.0	41.0	33.0	41.0
24	37.614092676988854	40.0	35.0	41.0	33.0	41.0
25	37.525511037216276	40.0	35.0	41.0	33.0	41.0
26	37.46077408963377	39.0	35.0	41.0	33.0	41.0
27	37.40396990929739	39.0	35.0	41.0	33.0	41.0
28	37.283835339875004	39.0	35.0	41.0	32.0	41.0
29	37.1821474696792	39.0	35.0	41.0	32.0	41.0
30	37.06844076124876	39.0	35.0	41.0	32.0	41.0
31	36.92525199293381	39.0	35.0	41.0	31.0	41.0
32	36.767320339058536	39.0	35.0	41.0	31.0	41.0
33	36.61309249884952	39.0	35.0	41.0	31.0	41.0
34	36.43907634754984	39.0	35.0	41.0	30.0	41.0
35	36.29081246381545	39.0	35.0	41.0	30.0	41.0
36	36.17691462672387	39.0	35.0	41.0	30.0	41.0
37	36.07083450855811	39.0	35.0	41.0	29.0	41.0
38	35.93474533497617	38.0	35.0	41.0	29.0	41.0
39	35.86725836141502	38.0	35.0	40.0	29.0	41.0
40	35.70698862877247	38.0	35.0	40.0	28.0	41.0
41	35.57053575404896	38.0	35.0	40.0	28.0	41.0
42	35.454355506732185	38.0	34.0	40.0	27.0	41.0
43	35.34382561643632	38.0	34.0	40.0	27.0	41.0
44	35.21323649481169	38.0	34.0	40.0	27.0	41.0
45	35.1149351275923	37.0	34.0	40.0	26.0	41.0
46	34.96136603179787	37.0	34.0	40.0	26.0	41.0
47	34.82157304454968	37.0	34.0	40.0	26.0	41.0
48	34.703117809479984	37.0	34.0	40.0	25.0	41.0
49	34.57986580170123	36.0	34.0	40.0	24.0	41.0
50	34.443283033712866	36.0	33.0	40.0	24.0	41.0
51	33.03811996199694	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	8.0
12	7.0
13	13.0
14	30.0
15	47.0
16	81.0
17	169.0
18	260.0
19	434.0
20	737.0
21	1164.0
22	1744.0
23	2490.0
24	3447.0
25	4304.0
26	5582.0
27	6518.0
28	7413.0
29	8470.0
30	10366.0
31	12643.0
32	16192.0
33	21951.0
34	37667.0
35	46708.0
36	46602.0
37	65422.0
38	101650.0
39	136724.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.474169796475806	21.026750590086547	25.768968127904042	10.730111485533602
2	33.729384083250444	25.339206983061917	24.481540311446935	16.449868622240697
3	31.957083265294006	25.338835859448068	26.43068153140448	16.273399343853452
4	27.967689978177933	26.904791948102076	26.13823612368808	18.989281950031916
5	24.4603862654573	30.58040021970518	25.033401125246797	19.925812389590725
6	24.668401051022073	37.19159627688791	24.007801018363196	14.132201653726822
7	82.00551489690187	6.8854564078203175	7.074543889078575	4.034484806199249
8	83.87839021421254	5.931297596603477	6.653875272775857	3.536436916408117
9	78.87582946127696	8.059877083859092	8.144493267817644	4.9198001870463015
10	41.89985600403783	26.675994240161515	15.29103513798376	16.1331146178169
11	32.53640722651901	27.10835325030061	20.666575122841916	19.688664400338464
12	27.462961863337444	24.16664192509241	27.989957395009128	20.380438816561018
13	25.772864925849504	25.247910574054007	28.346421626115227	20.632802873981266
14	22.261849976990337	28.801604738506303	27.81608598191886	21.120459302584504
15	22.0360212579606	26.273510680937605	30.355128186096223	21.335339875005566
16	24.29672075174799	23.892567136261746	29.94689221085759	21.86381990113267
17	23.77974555765034	23.6012350993869	26.771373008921813	25.84764633404094
18	25.497491204370355	24.57654795659338	27.297626293365795	22.628334545670473
19	26.271283939254488	26.166812641954785	25.727031159538623	21.834872259252112
20	26.995160548075354	25.18686073957514	25.669878123005212	22.1481005893443
21	26.220625565963513	25.4525852470941	26.870091890206787	21.456697296735598
22	25.211911583510236	23.86733073051972	25.772864925849504	25.147892760120538
23	24.502508795629648	27.55722726125618	25.229725516975194	22.710538426138978
24	23.296357050606417	26.68453008328014	27.441251131927025	22.57786173418642
25	24.63555661119606	26.4301248459837	25.740206047830412	23.19411249498983
26	23.24625536273622	26.770445199887178	25.895150156614164	24.088149280762437
27	23.789580333417454	25.709773911494437	26.491360242269497	24.00928551281861
28	22.415680714932527	27.476322313436157	27.6631830530113	22.444813918620014
29	23.607173077208557	26.549255526030613	27.013902290574944	22.829669106185886
30	24.037676469278388	26.499153838160417	26.95823374849695	22.50493594406425
31	26.822216944019715	26.727580422487122	24.005017591259296	22.445185042233867
32	25.658002167361904	27.154372578418418	24.642793521666196	22.54483173255348
33	25.428462212193637	27.09109600225643	23.772879770794056	23.707562014755876
34	23.36148924483767	27.580236925315084	25.753195374315276	23.30507845553197
35	24.43310867983908	26.891431498003353	25.310259341181364	23.365200480976203
36	25.737422620726512	28.35514303104078	23.918545789231477	21.988888559001232
37	25.209499280020186	27.897733176966582	24.9944331457922	21.898334397221024
38	25.30970265576058	27.646853614001753	24.46428306340276	22.57916066683491
39	25.310444902988287	25.672847111916035	25.46483232635126	23.551875658744414
40	24.773614595549486	25.65057969508484	27.569288778706415	22.006516930659263
41	22.223067559342667	26.00667280257708	26.89829728485964	24.87196235322061
42	24.342554518058876	26.38076540534121	25.68639312382168	23.590286952778232
43	25.43885367338153	24.084252482816975	26.838360821222334	23.63853302257916
44	24.454077164021793	25.5674480055817	26.40878523818714	23.56968959220937
45	25.33326900524027	25.205788043881654	25.805338242061666	23.655604708816412
46	25.32009411694847	25.9580556091623	25.500089069667325	23.221761204221902
47	23.494165936790225	26.84801003518252	26.873988688152252	22.783835339875004
48	23.872155337499816	27.24010213321853	25.8313168950314	23.056425634250253
49	25.002783427103896	26.04211510770007	26.319901132669273	22.635200332526757
50	22.9543666404406	26.768404020010983	26.846525540727107	23.430703798821312
51	23.848217864406276	25.55891216246307	24.861199768418864	25.731670204711786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1895.0
1	2051.5
2	2208.0
3	2540.0
4	2872.0
5	2191.5
6	1511.0
7	1529.0
8	1547.0
9	1596.5
10	1646.0
11	1741.5
12	1837.0
13	1932.0
14	2027.0
15	2020.5
16	2014.0
17	2224.5
18	2435.0
19	2091.0
20	1747.0
21	1789.5
22	1832.0
23	2103.0
24	2374.0
25	2993.0
26	4079.0
27	4546.0
28	5356.0
29	6166.0
30	7275.5
31	8385.0
32	9693.5
33	11002.0
34	11257.0
35	11512.0
36	13286.0
37	15060.0
38	15806.5
39	16553.0
40	17524.0
41	18495.0
42	21434.5
43	24374.0
44	26889.5
45	29405.0
46	32074.0
47	34743.0
48	36743.0
49	38743.0
50	39442.5
51	40142.0
52	38120.0
53	36098.0
54	34550.5
55	33003.0
56	31363.5
57	29724.0
58	28834.5
59	27945.0
60	26778.0
61	25611.0
62	24389.5
63	23168.0
64	20659.5
65	18151.0
66	16205.5
67	14260.0
68	12379.0
69	10498.0
70	9857.0
71	9216.0
72	8637.0
73	8058.0
74	6913.0
75	4676.5
76	3585.0
77	3022.0
78	2459.0
79	1871.5
80	1284.0
81	979.5
82	675.0
83	502.0
84	329.0
85	223.5
86	118.0
87	78.5
88	39.0
89	42.0
90	45.0
91	67.0
92	89.0
93	53.5
94	18.0
95	30.0
96	42.0
97	32.5
98	23.0
99	19.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538904.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.77965204455782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9931403961105	19.5907671718496
2	7.748633286368347	3.9951413988690905
3	2.845253157373011	2.2004890912726722
4	1.5729255245588005	1.621978908605179
5	1.0882681923914221	1.4027587665505388
6	0.7934498030635064	1.2272915902680073
7	0.655453631495381	1.1828156585904925
8	0.5650702553414338	1.1653851650745284
9	0.49003563440345227	1.1369653329919633
>10	7.128710246021468	45.09101347800933
>50	1.0491636670332292	17.08739785086206
>100	0.06269041142300201	2.7433461049250525
>500	0.0050440560915059095	0.835932927421637
>1k	0.002161738324931104	0.7187165547098475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1572	0.29170316048869555	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1221	0.22657096625744103	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1076	0.19966450425307664	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	886	0.1644077609370129	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	751	0.139356917001915	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	633	0.11746062378457016	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	622	0.11541944390837701	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	571	0.10595579175511778	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04917387883556255	0.0
2	0.0	0.0	0.0	0.25199293380639226	0.0
3	0.0	0.0	0.0	0.3479283879874709	0.0
4	0.0	0.0	0.0	0.4958211481080118	0.0
5	0.0	0.0	0.0	0.9541588112168401	0.0
6	0.0	0.0	0.0	1.2735106809376067	0.0
7	0.0	0.0	0.0	1.5615026052877692	0.0
8	0.0	0.0	0.0	2.0124177961195313	0.0
9	0.0	0.0	0.0	2.2363908970799993	0.0
10	0.0	0.0	0.0	2.78936508172142	0.0
11	0.0	0.0	0.0	3.5835696153674865	0.0
12	0.0	0.0	0.0	4.150089811914553	0.0
13	0.0	0.0	0.0	4.366083755177175	0.0
14	0.0	0.0	0.0	4.462019209358253	0.0
15	0.0	0.0	0.0	4.61844781259742	0.0
16	0.0	0.0	0.0	4.9572836720454845	0.0
17	0.0	0.0	0.0	5.359952793076318	0.0
18	0.0	0.0	0.0	5.844269109154878	0.0
19	0.0	0.0	0.0	6.1010866499413625	0.0
20	0.0	0.0	0.0	6.335822335703576	0.0
21	0.0	0.0	0.0	6.6145361697074065	0.0
22	0.0	0.0	0.0	6.911620622596975	0.0
23	0.0	0.0	0.0	7.189592209373098	0.0
24	0.0	0.0	0.0	7.44158514317949	0.0
25	0.0	0.0	0.0	7.649785490551193	0.0
26	0.0	0.0	0.0	7.856130219853629	0.0
27	0.0	0.0	0.0	8.078062140937904	0.0
28	0.0	0.0	0.0	8.301292994670664	0.0
29	0.0	0.0	0.0	8.520070365037187	0.0
30	0.0	0.0	0.0	8.753878241764767	0.0
31	0.0	0.0	0.0	8.978222466339089	0.0
32	1.8556180692665112E-4	0.0	0.0	9.216112702819055	0.0
33	1.8556180692665112E-4	0.0	0.0	9.426725353680805	0.0
34	1.8556180692665112E-4	0.0	0.0	9.641977049715718	0.0
35	1.8556180692665112E-4	0.0	0.0	9.8702180722355	0.0
36	1.8556180692665112E-4	0.0	0.0	10.091407746092068	0.0
37	1.8556180692665112E-4	0.0	0.0	10.321504386681116	0.0
38	1.8556180692665112E-4	0.0	0.0	10.535271588260619	0.0
39	1.8556180692665112E-4	0.0	0.0	10.776873060879117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGATCG	20	7.029947E-4	45.000004	21
ATTCGCG	20	7.029947E-4	45.000004	1
CCGTATT	20	7.029947E-4	45.000004	16
GTATGAT	20	7.029947E-4	45.000004	32
TGTTACG	20	7.029947E-4	45.000004	1
CATCCGA	20	7.029947E-4	45.000004	23
TAAGACG	20	7.029947E-4	45.000004	1
CTAACGG	25	3.888039E-5	45.0	2
ATATACG	25	3.888039E-5	45.0	1
TTAGTGC	25	3.888039E-5	45.0	14
ACGCATT	155	0.0	43.548386	17
CTACGCA	160	0.0	42.187504	15
AACGGGA	135	0.0	41.666664	4
TACGAAT	65	0.0	41.53846	12
TACGCAT	165	0.0	40.90909	16
CGCTACA	165	0.0	40.90909	28
TATGGGA	240	0.0	39.375004	4
TGGGCGA	385	0.0	39.15584	6
CGATTTA	35	6.242115E-6	38.571426	10
CTACGAA	70	0.0	38.571426	11
>>END_MODULE