FastQCFastQC Report
Sat 18 Jun 2016
SRR3553882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332262
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24450.7358650703360601No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12790.3849371881226261No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT9660.2907344204272532No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.2299390240232106No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5520.16613395452985896No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5010.15078462177438287No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4240.12761013898670326No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4190.12610530244204873No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA4150.12490143320632513No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCC4060.122192727425947No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.10864919852405631No Hit
AGTCTTTTTTTTTTTCTTTTATAGTAAACACACCCCCACCTCCATCCCAGC3410.10262985234543824No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC253.8847986E-545.00000433
TACGGGT253.8847986E-545.0000044
GTAATCG253.8847986E-545.0000041
CTAACGG253.8847986E-545.0000042
AATTCGT253.8847986E-545.00000431
TCGTACA253.8847986E-545.00000434
ACCACGG253.8847986E-545.0000042
TAGTAGC253.8847986E-545.00000432
TGATAAG207.0260395E-445.01
AGCGCTA207.0260395E-445.025
ATGACGC207.0260395E-445.02
CATCGGA207.0260395E-445.031
ATGCGAG207.0260395E-445.01
TCCCCCG207.0260395E-445.038
ATCATGC207.0260395E-445.034
CTGGCGT207.0260395E-445.017
ACGGTGT207.0260395E-445.022
CGTATGG302.1608266E-644.9999962
ATGTTAC600.044.99999620
AACACGT1150.043.0434841