##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332262
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27824126743353	33.0	31.0	34.0	30.0	34.0
2	32.427581246125044	34.0	31.0	34.0	31.0	34.0
3	32.46938259566246	34.0	31.0	34.0	31.0	34.0
4	35.97405360829707	37.0	35.0	37.0	35.0	37.0
5	35.88555116143284	37.0	35.0	37.0	35.0	37.0
6	35.86885951447954	37.0	35.0	37.0	35.0	37.0
7	35.927803961933655	37.0	35.0	37.0	35.0	37.0
8	35.81714430178594	37.0	35.0	37.0	35.0	37.0
9	37.66944760460119	39.0	38.0	39.0	35.0	39.0
10	37.248039197982315	39.0	37.0	39.0	34.0	39.0
11	37.14763349404988	39.0	37.0	39.0	34.0	39.0
12	36.9967345046981	39.0	37.0	39.0	33.0	39.0
13	36.89577502091723	39.0	37.0	39.0	33.0	39.0
14	38.09860591942503	40.0	37.0	41.0	33.0	41.0
15	38.210105278364665	40.0	37.0	41.0	33.0	41.0
16	38.26693091596391	40.0	37.0	41.0	34.0	41.0
17	38.199703848168014	40.0	37.0	41.0	34.0	41.0
18	38.15980762169613	40.0	37.0	41.0	34.0	41.0
19	38.06032588740211	40.0	36.0	41.0	34.0	41.0
20	37.99305668418297	40.0	36.0	41.0	34.0	41.0
21	37.88070558775906	40.0	36.0	41.0	33.0	41.0
22	37.836842612155465	40.0	36.0	41.0	33.0	41.0
23	37.74189946488013	40.0	35.0	41.0	33.0	41.0
24	37.68330112983128	40.0	35.0	41.0	33.0	41.0
25	37.57139847469768	40.0	35.0	41.0	33.0	41.0
26	37.51100035514143	40.0	35.0	41.0	33.0	41.0
27	37.461081917282144	40.0	35.0	41.0	33.0	41.0
28	37.38520203935448	39.0	35.0	41.0	33.0	41.0
29	37.287649505510714	39.0	35.0	41.0	33.0	41.0
30	37.18406558679596	39.0	35.0	41.0	32.0	41.0
31	37.06516243205663	39.0	35.0	41.0	32.0	41.0
32	36.92557680384757	39.0	35.0	41.0	31.0	41.0
33	36.80380844032721	39.0	35.0	41.0	31.0	41.0
34	36.73550691923843	39.0	35.0	41.0	31.0	41.0
35	36.550246492226016	39.0	35.0	41.0	31.0	41.0
36	36.40807555483323	39.0	35.0	41.0	30.0	41.0
37	36.32221861061451	39.0	35.0	41.0	30.0	41.0
38	36.16443047956131	38.0	35.0	41.0	30.0	41.0
39	36.120994877536404	38.0	35.0	41.0	30.0	41.0
40	35.98424737105056	38.0	35.0	40.0	29.0	41.0
41	35.81387278713786	38.0	35.0	40.0	29.0	41.0
42	35.67963233833541	38.0	35.0	40.0	28.0	41.0
43	35.57940119544215	38.0	35.0	40.0	28.0	41.0
44	35.41977114445829	38.0	34.0	40.0	28.0	41.0
45	35.31518801427789	37.0	34.0	40.0	27.0	41.0
46	35.169477701332085	37.0	34.0	40.0	27.0	41.0
47	35.043050363869476	37.0	34.0	40.0	26.0	41.0
48	34.879573950677475	37.0	34.0	40.0	26.0	41.0
49	34.8173790562869	37.0	34.0	40.0	26.0	41.0
50	34.747006880112686	36.0	34.0	40.0	26.0	41.0
51	33.3633247256683	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	2.0
12	5.0
13	5.0
14	9.0
15	23.0
16	41.0
17	74.0
18	170.0
19	265.0
20	372.0
21	605.0
22	865.0
23	1280.0
24	1750.0
25	2227.0
26	2889.0
27	3578.0
28	4264.0
29	5113.0
30	6180.0
31	7672.0
32	9901.0
33	13397.0
34	23416.0
35	30826.0
36	27353.0
37	38960.0
38	62241.0
39	88734.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.291739651239084	20.209051892783407	22.936718613624187	13.562489842353322
2	30.23908843021471	26.940486724332004	25.891314685398868	16.929110160054414
3	32.970366758762665	26.283776056244772	25.07117876856216	15.674678416430407
4	27.763030379640163	27.36003515298168	25.888305012309566	18.98862945506859
5	22.563218183240934	33.45131251843424	24.543583076006286	19.441886222318534
6	23.20789015897093	38.591232220356225	24.078889551016967	14.121988069655874
7	81.23348441892242	8.548976410182325	6.895762982224871	3.3217761886703867
8	83.11844267475666	6.551155413498985	6.925257778500099	3.405144133244247
9	78.4275661977596	9.407636142562195	7.674064443120187	4.490733216558017
10	40.194786042340084	27.785602927809983	15.897394225039276	16.12221680481066
11	31.04477791622274	28.203646519915004	20.775773335500297	19.975802228361957
12	27.49637334392738	25.041683972286933	27.765438118111614	19.69650456567408
13	24.004550625711037	27.654080213807177	28.14736563314493	20.19400352733686
14	22.161125858509248	29.467107282806943	27.588770307769174	20.78299655091464
15	20.99246979793055	28.545846350169445	29.32986618993445	21.131817661965556
16	23.01707688510874	24.12915109160843	30.89971167331804	21.954060349964788
17	22.976145331094138	24.580602055004785	27.660099559985795	24.78315305391528
18	24.59565042045133	26.036079960994634	26.492647368642817	22.875622249911213
19	24.766298884615153	28.66111682948998	24.499641848902375	22.07294243699249
20	26.471278689708722	26.756595698575218	24.617320066694358	22.1548055450217
21	25.9665565126316	28.072725740530064	24.223654826612734	21.737062920225604
22	24.056918937465014	25.25597269624573	24.91256899675557	25.774539369533684
23	22.97343662531376	29.291643341700222	24.25977090368444	23.475149129301577
24	23.07365873918775	28.0390174019298	27.455441789912783	21.431882068969667
25	24.319061463543832	28.296946385683587	24.967345046980995	22.416647103791586
26	22.32695884573018	27.644148292612456	25.335729033112425	24.693163828544943
27	22.663139329805997	28.13412307155197	26.527860543787735	22.6748770548543
28	22.028700242579653	27.656186984969693	28.598816596541283	21.71629617590937
29	22.516267283047718	28.44743004014904	25.87325664686302	23.16304602994023
30	23.514575846771525	27.61555639826402	27.505101395886378	21.36476635907808
31	25.739326194388767	28.002901324858094	23.414353732897535	22.84341874785561
32	24.77773564235453	29.06772366385563	24.50897183547923	21.64556885831061
33	24.05541410092036	28.941618361413585	22.55870367360697	24.444263864059085
34	22.46419993860267	28.214180375727587	25.02513077029573	24.296488915374013
35	24.550806291420628	26.80565336993096	24.62544618403549	24.018094154612925
36	25.152439941973505	29.330769091861242	23.70268041485334	21.814110551311916
37	23.316238390186058	29.952868519421422	23.894697557951254	22.836195532441266
38	24.257965099830855	30.003431027321813	24.410254558149894	21.328349314697437
39	23.83299925962042	27.250483052530832	23.87423178094395	25.042285906904795
40	24.419283577417822	26.950418645526725	26.608519782581215	22.02177799447424
41	21.81802312632802	27.035893361263096	26.52304506684484	24.623038445564045
42	22.37722038632164	28.894065526602503	24.824084607929887	23.904629479145974
43	23.30660743630027	26.284077023553703	25.63037602855578	24.778939511590252
44	23.701777512926544	25.816975760092937	26.684965479049666	23.79628124793085
45	24.91106416021092	27.100601332683244	24.883375167789275	23.104959339316565
46	24.759376636509742	26.67142195014777	25.069974899326436	23.499226514016048
47	22.90722381734896	29.03792790027147	26.101088899723713	21.953759382655853
48	22.946349567509976	28.520866063528178	25.865431496830816	22.66735287213103
49	22.823253938157237	29.026792109841026	25.409165056491563	22.74078889551017
50	22.563218183240934	26.780974050598623	27.42474312440183	23.23106464175861
51	22.398890032564665	28.223209394995514	24.759677603818673	24.618222968621147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4146.0
1	3640.0
2	3134.0
3	2682.5
4	2231.0
5	1662.0
6	1093.0
7	1382.5
8	1672.0
9	1867.0
10	2062.0
11	1767.5
12	1473.0
13	1368.0
14	1263.0
15	1249.5
16	1236.0
17	1175.0
18	1114.0
19	986.5
20	859.0
21	1014.0
22	1169.0
23	1210.5
24	1252.0
25	1709.5
26	2260.0
27	2353.0
28	3079.5
29	3806.0
30	4162.0
31	4518.0
32	5357.5
33	6197.0
34	7170.5
35	8144.0
36	8357.5
37	8571.0
38	9081.5
39	9592.0
40	11243.0
41	12894.0
42	14174.0
43	15454.0
44	17061.0
45	18668.0
46	19654.0
47	20640.0
48	21802.5
49	22965.0
50	23233.5
51	23502.0
52	21885.5
53	20269.0
54	19143.0
55	18017.0
56	17541.5
57	17066.0
58	17147.5
59	17229.0
60	16708.5
61	16188.0
62	14894.5
63	13601.0
64	12617.5
65	11634.0
66	10709.0
67	9784.0
68	8719.5
69	7655.0
70	6671.0
71	5687.0
72	5158.0
73	4629.0
74	3927.5
75	2783.5
76	2341.0
77	1794.5
78	1248.0
79	1028.5
80	809.0
81	621.0
82	433.0
83	286.0
84	139.0
85	115.0
86	91.0
87	55.0
88	19.0
89	15.0
90	11.0
91	7.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	332262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.878487458692234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47464364495504	19.748872877428052
2	7.35216724332919	3.9523027008806304
3	2.9807293941124438	2.4035249291221987
4	1.906905393754129	2.0501893084373175
5	1.3929479212155822	1.8720166615502225
6	1.1074159920275006	1.7859400111959838
7	0.9248995039582564	1.7401929802384863
8	0.7994894017266284	1.7191252686133232
9	0.6886358292183143	1.6658540549325533
>10	8.99369590289675	53.05662398950226
>50	0.3112857894677909	5.104104592159199
>100	0.060465585004534916	2.9428583467263785
>500	0.004478932222558143	0.8375920207547056
>1k	0.0022394661112790713	1.120802258458686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2445	0.7358650703360601	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1279	0.3849371881226261	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	966	0.2907344204272532	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.2299390240232106	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	552	0.16613395452985896	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	501	0.15078462177438287	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	424	0.12761013898670326	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	419	0.12610530244204873	No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA	415	0.12490143320632513	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCC	406	0.122192727425947	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	361	0.10864919852405631	No Hit
AGTCTTTTTTTTTTTCTTTTATAGTAAACACACCCCCACCTCCATCCCAGC	341	0.10262985234543824	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0096730893090394E-4	0.0	0.0	0.06079539640404259	0.0
2	3.0096730893090394E-4	0.0	0.0	0.26304542800561004	0.0
3	3.0096730893090394E-4	0.0	0.0	0.3346756475311652	0.0
4	3.0096730893090394E-4	0.0	0.0	0.43188808831584713	0.0
5	3.0096730893090394E-4	0.0	0.0	0.7888353167078992	0.0
6	3.0096730893090394E-4	0.0	0.0	1.109064533410381	0.0
7	3.0096730893090394E-4	0.0	0.0	1.3528480536444132	0.0
8	3.0096730893090394E-4	0.0	0.0	1.7116010858900506	0.0
9	3.0096730893090394E-4	0.0	0.0	1.8723176288591534	0.0
10	3.0096730893090394E-4	0.0	0.0	2.255449013128194	0.0
11	3.0096730893090394E-4	0.0	0.0	2.8236752923897406	0.0
12	3.0096730893090394E-4	0.0	0.0	3.217942467089225	0.0
13	3.0096730893090394E-4	0.0	0.0	3.374746435042226	0.0
14	3.0096730893090394E-4	0.0	0.0	3.4370466679909226	0.0
15	3.0096730893090394E-4	0.0	0.0	3.537268781864914	0.0
16	3.0096730893090394E-4	0.0	0.0	3.792790027147251	0.0
17	3.0096730893090394E-4	0.0	0.0	4.091349597606708	0.0
18	3.0096730893090394E-4	0.0	0.0	4.431442656698629	0.0
19	3.0096730893090394E-4	0.0	0.0	4.61804238823579	0.0
20	3.0096730893090394E-4	0.0	0.0	4.770632813863758	0.0
21	3.0096730893090394E-4	0.0	0.0	4.961747055034882	0.0
22	3.0096730893090394E-4	0.0	0.0	5.18085125593658	0.0
23	3.0096730893090394E-4	0.0	0.0	5.42704251464206	0.0
24	3.0096730893090394E-4	0.0	0.0	5.638923500129416	0.0
25	6.019346178618079E-4	0.0	0.0	5.833950316316642	0.0
26	6.019346178618079E-4	0.0	0.0	5.993763957358952	0.0
27	6.019346178618079E-4	0.0	0.0	6.176752081188941	0.0
28	6.019346178618079E-4	0.0	0.0	6.359740205018931	0.0
29	6.019346178618079E-4	0.0	0.0	6.537611884597095	0.0
30	6.019346178618079E-4	0.0	0.0	6.740463850816524	0.0
31	6.019346178618079E-4	0.0	0.0	6.918636497703619	0.0
32	6.019346178618079E-4	0.0	0.0	7.109750738874744	0.0
33	6.019346178618079E-4	0.0	0.0	7.308991097387002	0.0
34	6.019346178618079E-4	0.0	0.0	7.492882123143784	0.0
35	6.019346178618079E-4	0.0	0.0	7.7198114740776855	0.0
36	6.019346178618079E-4	0.0	0.0	7.916343126809566	0.0
37	6.019346178618079E-4	0.0	0.0	8.11738928917541	0.0
38	6.019346178618079E-4	0.0	0.0	8.322648993866286	0.0
39	6.019346178618079E-4	0.0	0.0	8.511656463874894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	25	3.8847986E-5	45.000004	33
TACGGGT	25	3.8847986E-5	45.000004	4
GTAATCG	25	3.8847986E-5	45.000004	1
CTAACGG	25	3.8847986E-5	45.000004	2
AATTCGT	25	3.8847986E-5	45.000004	31
TCGTACA	25	3.8847986E-5	45.000004	34
ACCACGG	25	3.8847986E-5	45.000004	2
TAGTAGC	25	3.8847986E-5	45.000004	32
TGATAAG	20	7.0260395E-4	45.0	1
AGCGCTA	20	7.0260395E-4	45.0	25
ATGACGC	20	7.0260395E-4	45.0	2
CATCGGA	20	7.0260395E-4	45.0	31
ATGCGAG	20	7.0260395E-4	45.0	1
TCCCCCG	20	7.0260395E-4	45.0	38
ATCATGC	20	7.0260395E-4	45.0	34
CTGGCGT	20	7.0260395E-4	45.0	17
ACGGTGT	20	7.0260395E-4	45.0	22
CGTATGG	30	2.1608266E-6	44.999996	2
ATGTTAC	60	0.0	44.999996	20
AACACGT	115	0.0	43.04348	41
>>END_MODULE