SRR3553879_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329747
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4638	1.4065328873348355	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1927	0.5843874242980224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1072	0.32509772643875456	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	1065	0.32297488680715825	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	899	0.27263326125787346	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	740	0.22441447534018505	TruSeq Adapter, Index 20 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	734	0.22259489851310246	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	540	0.16376191443743235	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	539	0.16345865163291856	No Hit
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	527	0.15981949797875342	TruSeq Adapter, Index 20 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	463	0.14041067848987254	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	454	0.13768131324924868	No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	410	0.12433774985064307	TruSeq Adapter, Index 27 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	408	0.12373122424161555	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	404	0.1225181730235605	No Hit
GAAAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT	361	0.10947787242946866	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	361	0.10947787242946866	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	355	0.10765829560238607	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	349	0.1058387187753035	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	332	0.1006832510985695	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	7.0259615E-4	45.0	30
CGGAATT	20	7.0259615E-4	45.0	17
AGCGTGA	20	7.0259615E-4	45.0	15
CATATCG	20	7.0259615E-4	45.0	32
GGTCGTG	20	7.0259615E-4	45.0	32
ATCTACC	35	1.2084092E-7	45.0	15
ACGCATT	45	3.8380676E-10	45.0	17
TAGACTA	20	7.0259615E-4	45.0	43
ACTTGGC	25	3.884735E-5	45.0	16
CTATCAA	20	7.0259615E-4	45.0	37
TATGGGC	70	0.0	45.0	4
GGCCGTA	20	7.0259615E-4	45.0	42
GAATCAA	20	7.0259615E-4	45.0	38
GTTAGCG	20	7.0259615E-4	45.0	1
CGCGATT	20	7.0259615E-4	45.0	25
CGACACG	25	3.884735E-5	45.0	38
CCGTCAA	25	3.884735E-5	45.0	13
CGTTATG	20	7.0259615E-4	45.0	2
ATATCGA	20	7.0259615E-4	45.0	33
CCCGTCA	25	3.884735E-5	45.0	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content