##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329747
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07197942665134	33.0	31.0	34.0	30.0	34.0
2	32.204960166430624	34.0	31.0	34.0	30.0	34.0
3	32.254325285749374	34.0	31.0	34.0	30.0	34.0
4	35.79706562910353	37.0	35.0	37.0	35.0	37.0
5	35.71059933828056	37.0	35.0	37.0	33.0	37.0
6	35.68376967796523	37.0	35.0	37.0	33.0	37.0
7	35.73208550798036	37.0	35.0	37.0	35.0	37.0
8	35.53061589642969	37.0	35.0	37.0	33.0	37.0
9	37.44368864614386	39.0	37.0	39.0	35.0	39.0
10	36.97909002962878	39.0	37.0	39.0	33.0	39.0
11	36.927004642953534	39.0	37.0	39.0	33.0	39.0
12	36.79160083336619	39.0	35.0	39.0	33.0	39.0
13	36.66449732673838	39.0	35.0	39.0	33.0	39.0
14	37.804662362356595	40.0	37.0	41.0	33.0	41.0
15	37.9200144353095	40.0	37.0	41.0	33.0	41.0
16	38.00497654262207	40.0	37.0	41.0	33.0	41.0
17	37.89711809356871	40.0	36.0	41.0	33.0	41.0
18	37.85525569603362	39.0	36.0	41.0	33.0	41.0
19	37.7045219516781	39.0	36.0	41.0	33.0	41.0
20	37.577200095831046	39.0	35.0	41.0	33.0	41.0
21	37.4669913600427	39.0	35.0	41.0	33.0	41.0
22	37.4130409071197	39.0	35.0	41.0	33.0	41.0
23	37.28693816774679	39.0	35.0	41.0	32.0	41.0
24	37.261630886710115	39.0	35.0	41.0	32.0	41.0
25	37.21721501636103	39.0	35.0	41.0	32.0	41.0
26	37.12188132113408	39.0	35.0	41.0	32.0	41.0
27	37.063172674808264	39.0	35.0	41.0	32.0	41.0
28	36.97699751627763	39.0	35.0	41.0	32.0	41.0
29	36.85963481093081	39.0	35.0	41.0	31.0	41.0
30	36.73928496695952	39.0	35.0	41.0	31.0	41.0
31	36.61517466421226	39.0	35.0	41.0	31.0	41.0
32	36.46894437250377	39.0	35.0	41.0	31.0	41.0
33	36.3300257470121	39.0	35.0	41.0	30.0	41.0
34	36.22929094123677	38.0	35.0	41.0	30.0	41.0
35	36.04216262771155	38.0	35.0	41.0	30.0	41.0
36	35.93436786384713	38.0	35.0	40.0	29.0	41.0
37	35.803270386083874	38.0	35.0	40.0	29.0	41.0
38	35.69973343199483	38.0	35.0	40.0	28.0	41.0
39	35.61959320327403	38.0	35.0	40.0	28.0	41.0
40	35.440176862867595	38.0	34.0	40.0	27.0	41.0
41	35.3183016069896	38.0	34.0	40.0	26.0	41.0
42	35.17648378908679	38.0	34.0	40.0	26.0	41.0
43	35.0829211486382	38.0	34.0	40.0	26.0	41.0
44	34.93855895580551	37.0	34.0	40.0	25.0	41.0
45	34.85913442730336	37.0	34.0	40.0	25.0	41.0
46	34.70000940114694	37.0	34.0	40.0	24.0	41.0
47	34.59476811009653	37.0	34.0	40.0	24.0	41.0
48	34.401880835913595	37.0	33.0	40.0	23.0	41.0
49	34.350380746451066	36.0	33.0	40.0	24.0	41.0
50	34.20671909069681	36.0	33.0	40.0	24.0	41.0
51	32.871161830130376	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	3.0
13	4.0
14	7.0
15	19.0
16	52.0
17	91.0
18	210.0
19	384.0
20	663.0
21	996.0
22	1525.0
23	1972.0
24	2511.0
25	2999.0
26	3481.0
27	4142.0
28	4898.0
29	5690.0
30	6871.0
31	8341.0
32	10698.0
33	14767.0
34	24080.0
35	32403.0
36	27209.0
37	37619.0
38	59794.0
39	78292.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.897915674744574	19.554385635047474	21.337267662783894	13.210431027424056
2	29.935677959162632	29.189044934449743	23.7545754775631	17.120701628824524
3	30.755397319763333	29.32278383124031	24.84783788783522	15.073980961161132
4	26.211913982538128	29.818315253815804	25.819188650692805	18.150582112953266
5	23.609615857005522	33.738593527765225	24.049043660745966	18.602746954483283
6	22.23249946170852	39.80324309243147	24.160947635611542	13.803309810248463
7	77.17280217863998	10.088946980563888	8.66694769019885	4.071303150597276
8	78.7794885169539	9.775373240696656	6.9641270428540665	4.481011199495371
9	74.1347154030211	10.673334404861908	8.703035963935987	6.488914228180999
10	38.39216126302893	28.130961009501227	17.225630559186285	16.251247168283562
11	31.06684821999897	28.80177833308567	21.15167082642147	18.979702620493892
12	27.216320391087713	25.33609100310238	27.462569788352887	19.98501881745702
13	23.571708006441302	28.77964014835616	28.217997434396676	19.43065441080586
14	20.208523504383663	32.281719014881105	26.852405025671196	20.657352455064036
15	19.285998053052793	29.817405465402263	30.62287147419082	20.273725007354123
16	21.84553612314896	25.492271347426964	30.500050038362748	22.162142491061328
17	20.657049192259517	27.114424088771088	27.111694723530466	25.116831995438925
18	21.816119631111125	28.046653949846394	27.15415151616239	22.983074902880087
19	24.024176110775837	29.818921779424834	24.25101668855213	21.9058854212472
20	24.48756167607287	28.912469256733193	25.048901127227847	21.551067939966096
21	24.372928335966666	30.56434175291966	24.833281273218557	20.229448637895114
22	22.23795819218977	28.003287368800926	24.79143100619566	24.967323432813643
23	21.312703375618277	30.894291684230634	24.574901363772835	23.218103576378255
24	21.63203910877127	29.712173272235987	27.36522242810397	21.29056519088877
25	22.708925327599644	30.56707111816029	24.94306240845254	21.780941145787526
26	19.21806718484171	30.06759727912612	25.587799130848804	25.12653640518337
27	22.526057856477845	29.20329828626189	25.41342301825339	22.857220839006875
28	20.800492498794533	30.735988500274452	26.711084558767784	21.752434442163235
29	22.385647177987973	28.599805305279503	25.480747360855442	23.53380015587708
30	22.770487676915938	29.10504113759943	25.959599329182677	22.16487185630195
31	24.782939647669274	30.248645173420837	22.512411030274727	22.456004148635166
32	24.47573442669683	30.38056449338432	23.015220760158545	22.128480319760303
33	23.379439388379573	28.51489172001565	23.1817120398366	24.92395685176817
34	21.525290601582427	28.866979836056128	25.828589797632727	23.779139764728715
35	22.56275265582401	28.694120037483284	24.22341977334138	24.519707533351326
36	24.83115843358696	28.781459725183247	23.793089853736348	22.594291987493442
37	22.285267189693915	30.51915559504711	25.305158197041976	21.890419018216996
38	22.604602922846908	31.01711312005871	24.207650107506666	22.170633849587716
39	22.824468456119387	27.691836468565295	25.062547953430965	24.421147121884353
40	22.430833335860523	27.101383788176996	28.46727945970699	22.000503416255494
41	20.88752892368998	27.931414084131166	25.882267314031665	25.298789678147187
42	21.7406071927872	28.348703703142107	25.783403639760177	24.127285464310518
43	23.30635305249176	26.680151752707378	25.809484240948365	24.2040109538525
44	23.027957797948122	27.006159267559678	26.042086812010417	23.92379612248178
45	22.56608854667366	27.118063242425254	26.386593357938054	23.92925485296303
46	23.826752025037376	28.668645961904126	25.65451694784183	21.850085065216664
47	23.155328175843902	26.597967532684148	27.469241570052194	22.777462721419756
48	21.878895031645474	27.641798105820527	26.74323041604624	23.73607644648776
49	22.488150005913624	26.96309594931872	26.878485626859376	23.670268417908275
50	22.449635629740378	25.881357525618125	28.150369828990108	23.518637015651393
51	21.8045956445396	27.23451615935854	26.35869317992279	24.60219501617907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5670.0
1	5057.0
2	4444.0
3	3713.5
4	2983.0
5	2180.5
6	1378.0
7	1420.0
8	1462.0
9	1516.0
10	1570.0
11	1620.5
12	1671.0
13	1573.5
14	1476.0
15	1400.5
16	1325.0
17	1233.0
18	1141.0
19	1094.0
20	1047.0
21	1238.0
22	1429.0
23	1335.0
24	1241.0
25	1509.0
26	1929.0
27	2081.0
28	2720.0
29	3359.0
30	4198.0
31	5037.0
32	5550.5
33	6064.0
34	5976.0
35	5888.0
36	6251.5
37	6615.0
38	7875.5
39	9136.0
40	10422.5
41	11709.0
42	13518.5
43	15328.0
44	18158.0
45	20988.0
46	23980.5
47	26973.0
48	27543.0
49	28113.0
50	27024.0
51	25935.0
52	24268.5
53	22602.0
54	20436.5
55	18271.0
56	17344.5
57	16418.0
58	15641.0
59	14864.0
60	14069.5
61	13275.0
62	12034.5
63	10794.0
64	9836.5
65	8879.0
66	7532.5
67	6186.0
68	6132.5
69	6079.0
70	5376.5
71	4674.0
72	4425.5
73	4177.0
74	3561.5
75	2334.5
76	1723.0
77	1402.0
78	1081.0
79	962.0
80	843.0
81	708.0
82	573.0
83	428.5
84	284.0
85	196.0
86	108.0
87	73.0
88	38.0
89	31.0
90	24.0
91	27.0
92	30.0
93	31.0
94	32.0
95	18.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.345528541578847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39873509290453	22.419612612093516
2	8.266333216212306	4.85159834661119
3	2.979352251823988	2.622919996239541
4	1.70824463137879	2.005173663445005
5	1.1460637000599385	1.681592251028819
6	0.8484385011264287	1.4938725750347994
7	0.7264948432300602	1.4923562610122307
8	0.580782506252196	1.363469569093881
9	0.4722733191410206	1.2473199149651095
>10	6.271831015025938	43.507598249567096
>50	0.5218775189632722	9.42692427831034
>100	0.06923919558522622	4.0418866585594415
>500	0.006200524977781452	1.2066826991602653
>1k	0.004133683318520968	2.638992924878771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4638	1.4065328873348355	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1927	0.5843874242980224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1072	0.32509772643875456	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	1065	0.32297488680715825	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	899	0.27263326125787346	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	740	0.22441447534018505	TruSeq Adapter, Index 20 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	734	0.22259489851310246	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	540	0.16376191443743235	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	539	0.16345865163291856	No Hit
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	527	0.15981949797875342	TruSeq Adapter, Index 20 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	463	0.14041067848987254	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	454	0.13768131324924868	No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	410	0.12433774985064307	TruSeq Adapter, Index 27 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	408	0.12373122424161555	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	404	0.1225181730235605	No Hit
GAAAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT	361	0.10947787242946866	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	361	0.10947787242946866	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	355	0.10765829560238607	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	349	0.1058387187753035	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	332	0.1006832510985695	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12828016630932199	0.0
2	0.0	0.0	0.0	0.6480726132459128	0.0
3	0.0	0.0	0.0	0.8097116880517488	0.0
4	0.0	0.0	0.0	1.0962950383172554	0.0
5	0.0	0.0	0.0	2.2244326711084557	0.0
6	0.0	0.0	0.0	3.0159485908893786	0.0
7	0.0	0.0	0.0	3.6797908699700073	0.0
8	0.0	0.0	0.0	4.53256587626271	0.0
9	3.032628045137636E-4	0.0	0.0	4.897391030092768	0.0
10	3.032628045137636E-4	0.0	0.0	6.036749386650978	0.0
11	3.032628045137636E-4	0.0	0.0	6.991420695260306	0.0
12	3.032628045137636E-4	0.0	0.0	7.8890785966210455	0.0
13	3.032628045137636E-4	0.0	0.0	8.190825087112241	0.0
14	3.032628045137636E-4	0.0	0.0	8.316679150985452	0.0
15	3.032628045137636E-4	0.0	0.0	8.52835658853606	0.0
16	3.032628045137636E-4	0.0	0.0	8.941400528283806	0.0
17	3.032628045137636E-4	0.0	0.0	9.437538476468323	0.0
18	3.032628045137636E-4	0.0	0.0	10.038605355014601	0.0
19	3.032628045137636E-4	0.0	0.0	10.355514985731485	0.0
20	3.032628045137636E-4	0.0	0.0	10.654228848177542	0.0
21	3.032628045137636E-4	0.0	0.0	10.95870470390936	0.0
22	3.032628045137636E-4	0.0	0.0	11.30351451264151	0.0
23	3.032628045137636E-4	0.0	0.0	11.645291693328522	0.0
24	3.032628045137636E-4	0.0	0.0	11.932481569203055	0.0
25	3.032628045137636E-4	0.0	0.0	12.155379730520671	0.0
26	3.032628045137636E-4	0.0	0.0	12.395260608891059	0.0
27	6.065256090275272E-4	0.0	0.0	12.649091576269079	0.0
28	6.065256090275272E-4	0.0	0.0	12.902316018038071	0.0
29	6.065256090275272E-4	0.0	0.0	13.146745838476164	0.0
30	6.065256090275272E-4	0.0	0.0	13.429690035087507	0.0
31	9.097884135412908E-4	0.0	0.0	13.681701425638444	0.0
32	9.097884135412908E-4	0.0	0.0	13.967374987490409	0.0
33	9.097884135412908E-4	0.0	0.0	14.23333646704898	0.0
34	9.097884135412908E-4	0.0	0.0	14.488077222840541	0.0
35	9.097884135412908E-4	0.0	0.0	14.800741174294231	0.0
36	9.097884135412908E-4	0.0	0.0	15.060940660567042	0.0
37	9.097884135412908E-4	0.0	0.0	15.320836884035336	0.0
38	9.097884135412908E-4	0.0	0.0	15.599232138578971	0.0
39	9.097884135412908E-4	0.0	0.0	15.853063105956991	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	7.0259615E-4	45.0	30
CGGAATT	20	7.0259615E-4	45.0	17
AGCGTGA	20	7.0259615E-4	45.0	15
CATATCG	20	7.0259615E-4	45.0	32
GGTCGTG	20	7.0259615E-4	45.0	32
ATCTACC	35	1.2084092E-7	45.0	15
ACGCATT	45	3.8380676E-10	45.0	17
TAGACTA	20	7.0259615E-4	45.0	43
ACTTGGC	25	3.884735E-5	45.0	16
CTATCAA	20	7.0259615E-4	45.0	37
TATGGGC	70	0.0	45.0	4
GGCCGTA	20	7.0259615E-4	45.0	42
GAATCAA	20	7.0259615E-4	45.0	38
GTTAGCG	20	7.0259615E-4	45.0	1
CGCGATT	20	7.0259615E-4	45.0	25
CGACACG	25	3.884735E-5	45.0	38
CCGTCAA	25	3.884735E-5	45.0	13
CGTTATG	20	7.0259615E-4	45.0	2
ATATCGA	20	7.0259615E-4	45.0	33
CCCGTCA	25	3.884735E-5	45.0	12
>>END_MODULE