SRR3553876_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380796
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATGTATATATATGTATATATACATATATATGTATATATACATATATATGTA	12072	3.1702013676614254	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2929	0.7691782476706688	No Hit
ATGTATATATACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGC	2314	0.6076744503618735	No Hit
ATGTATATATACATATATATATACATATATATGTATATATACATATATATG	1419	0.3726404689124886	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1381	0.3626613724934085	No Hit
ATGTATATATACATATATATGTATATATACATATATATGTATATATACATA	1062	0.2788894841332367	No Hit
ATGTATATATATGTATATATACATATATATGCTGTCTCTTATACACATCTG	927	0.24343743106545238	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	870	0.2284687864368323	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACATATATATACA	857	0.22505488503030496	No Hit
ATGTATATATATGTATATATACATATATATCTGTCTCTTATACACATCTGA	722	0.18960283196252062	No Hit
ATGTATATATATGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTAT	645	0.16938203132385843	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCC	631	0.16570552211682896	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACATATATATGCT	627	0.16465509091482053	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACCTGTCTCTTAT	538	0.1412829966701331	No Hit
ATGTATATATATGTATATATACCTGTCTCTTATACACATCTGACGCGATTC	505	0.13261693925356358	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.12788999884452568	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	444	0.11659786342293511	No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	416	0.10924484500887614	No Hit
ATGTATATATATGTATATATACATATATACTGTCTCTTATACACATCTGAC	400	0.10504312020084244	No Hit
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	391	0.10267964999632348	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACATATATATCTG	381	0.10005357199130242	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	30	2.1616634E-6	45.000004	1
CACGGGT	35	1.209064E-7	45.000004	4
GCAACTC	25	3.8858747E-5	45.000004	11
ATAGCGG	25	3.8858747E-5	45.000004	2
CAATTGG	35	1.209064E-7	45.000004	2
ACCAGTC	25	3.8858747E-5	45.000004	13
TCGTATA	25	3.8858747E-5	45.000004	29
TTAGTAG	30	2.1616634E-6	45.000004	1
CAATGCG	25	3.8858747E-5	45.000004	1
GCGTAAG	25	3.8858747E-5	45.000004	1
GTAGTAG	25	3.8858747E-5	45.000004	1
CCGTACT	20	7.0273376E-4	45.0	44
CCGTAAT	20	7.0273376E-4	45.0	27
CGTAGTG	20	7.0273376E-4	45.0	28
TACGAAC	20	7.0273376E-4	45.0	12
CGTACTC	20	7.0273376E-4	45.0	45
GAACGCC	20	7.0273376E-4	45.0	36
CTCGACG	20	7.0273376E-4	45.0	13
ACCGTAA	20	7.0273376E-4	45.0	26
GCGTGAA	20	7.0273376E-4	45.0	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content