##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380796
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.056589354930196	33.0	31.0	34.0	30.0	34.0
2	32.26095074528094	34.0	31.0	34.0	30.0	34.0
3	32.329509763758026	34.0	31.0	34.0	30.0	34.0
4	35.74664912446559	37.0	35.0	37.0	35.0	37.0
5	35.68470519648316	37.0	35.0	37.0	33.0	37.0
6	35.728484542904866	37.0	35.0	37.0	33.0	37.0
7	35.57897929600101	37.0	35.0	37.0	33.0	37.0
8	35.67948192733117	37.0	35.0	37.0	35.0	37.0
9	37.41028792319247	39.0	37.0	39.0	34.0	39.0
10	37.16026954064643	39.0	37.0	39.0	34.0	39.0
11	37.03104024201935	39.0	37.0	39.0	33.0	39.0
12	37.018230233510856	39.0	37.0	39.0	33.0	39.0
13	36.93380970388345	39.0	37.0	39.0	33.0	39.0
14	38.012423975041756	40.0	37.0	41.0	33.0	41.0
15	38.09239855460667	40.0	37.0	41.0	33.0	41.0
16	38.25208247985798	40.0	37.0	41.0	34.0	41.0
17	38.18004916018025	40.0	37.0	41.0	34.0	41.0
18	38.14187649029927	40.0	37.0	41.0	34.0	41.0
19	38.05739556087774	40.0	37.0	41.0	34.0	41.0
20	38.0159009023204	40.0	36.0	41.0	33.0	41.0
21	37.873152554123465	40.0	36.0	41.0	33.0	41.0
22	37.8790822382588	40.0	36.0	41.0	33.0	41.0
23	37.76197491570289	40.0	36.0	41.0	33.0	41.0
24	37.761355161293714	40.0	36.0	41.0	33.0	41.0
25	37.62764314751205	40.0	35.0	41.0	33.0	41.0
26	37.60937352283112	40.0	35.0	41.0	33.0	41.0
27	37.51231367976554	40.0	35.0	41.0	33.0	41.0
28	37.46871815880419	40.0	35.0	41.0	33.0	41.0
29	37.33806552589838	39.0	35.0	41.0	32.0	41.0
30	37.22942730490867	39.0	35.0	41.0	32.0	41.0
31	37.118722360529	39.0	35.0	41.0	32.0	41.0
32	36.94122312209162	39.0	35.0	41.0	31.0	41.0
33	36.868380970388344	39.0	35.0	41.0	31.0	41.0
34	36.76912047395456	39.0	35.0	41.0	31.0	41.0
35	36.62213363585752	39.0	35.0	41.0	31.0	41.0
36	36.53979296001008	39.0	35.0	41.0	30.0	41.0
37	36.44807455960672	39.0	35.0	41.0	30.0	41.0
38	36.38508545257828	39.0	35.0	41.0	30.0	41.0
39	36.29112700763663	39.0	35.0	41.0	30.0	41.0
40	36.1557526864778	39.0	35.0	41.0	30.0	41.0
41	35.98302503177555	38.0	35.0	40.0	29.0	41.0
42	35.90709461233836	38.0	35.0	40.0	29.0	41.0
43	35.7879179403145	38.0	35.0	40.0	28.0	41.0
44	35.71374174098467	38.0	35.0	40.0	28.0	41.0
45	35.605326736625386	38.0	35.0	40.0	28.0	41.0
46	35.495656466979696	38.0	34.0	40.0	27.0	41.0
47	35.333861175012345	38.0	34.0	40.0	27.0	41.0
48	35.19581088036639	38.0	34.0	40.0	26.0	41.0
49	35.10451790459984	37.0	34.0	40.0	26.0	41.0
50	34.974997111314195	37.0	34.0	40.0	26.0	41.0
51	33.6407446506791	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	1.0
12	1.0
13	4.0
14	18.0
15	23.0
16	50.0
17	93.0
18	162.0
19	291.0
20	486.0
21	739.0
22	1082.0
23	1535.0
24	2112.0
25	2719.0
26	3675.0
27	4269.0
28	4894.0
29	5658.0
30	6754.0
31	8474.0
32	10844.0
33	14710.0
34	24782.0
35	32399.0
36	31733.0
37	45119.0
38	74031.0
39	104105.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.75803317261736	27.928864798999992	20.205044170632043	12.108057857750607
2	28.152606645027785	23.70823222933014	32.647401758421836	15.491759367220245
3	38.942110736457316	23.732392146976334	23.252345087658483	14.073152028907868
4	24.87263521675648	25.7607747980546	32.54656036302902	16.820029622159897
5	20.92406432840681	39.39694744692696	22.04014748054076	17.638840744125464
6	21.227638945787245	34.24405718547464	31.589354930198848	12.938948938539271
7	73.03359278984023	17.19083183646887	6.4338911123016	3.3416842613893003
8	74.40309246945871	5.962247502599817	16.088141682160526	3.5465183457809433
9	69.92195296169076	18.184014537967837	7.598556707528441	4.29547579281295
10	34.74721373123667	25.95799325623168	24.25681992457904	15.037973087952604
11	27.234267166671916	35.65793758337797	19.75414657716993	17.353648672780174
12	24.5509406611414	22.263626718768055	32.269509133499305	20.915923486591247
13	28.620311137722037	26.207733274509188	26.97612369877835	18.19583188899043
14	20.092648032017145	26.903118730238763	34.214645111818406	18.789588125925693
15	18.309803674408347	34.6881269761237	28.489269845271487	18.512799504196472
16	20.543808233279762	22.57376653116104	36.71309572579544	20.16932950976376
17	20.088971522810116	31.380844336600173	26.48898622884694	22.04119791174277
18	20.653840901690142	24.57851448019412	33.79263437641152	20.97501024170422
19	22.71688778243469	34.226987678442	23.31799703778401	19.7381275013393
20	22.758642422714527	24.829830145274634	32.034737759850415	20.376789672160424
21	22.72161472284373	34.113015893024084	23.80434668431391	19.361022699818275
22	20.81324383659492	23.82299183814956	25.263920839504618	30.0998434857509
23	20.99260496433786	35.247481591193186	23.050137081271863	20.70977636319709
24	20.41407997983172	25.88446307209109	32.3325350056198	21.368921942457376
25	22.26283889536655	33.79263437641152	22.841889095473693	21.10263763274824
26	19.869956617191356	26.284939967856808	30.864820008613535	22.9802834063383
27	21.23814325780733	33.5533986701541	24.09347787266673	21.114980199371843
28	19.95635458355655	25.548587695248898	33.6665826321705	20.828475089024046
29	21.35264025882625	33.698095568230755	23.929347997352913	21.01991617559008
30	21.73210853055179	25.15677685689976	32.03841426905745	21.072700343491
31	29.208815218647256	27.487946301956956	22.031744030924692	21.2714944484711
32	22.19114696582947	26.970083719366798	30.365077364258024	20.4736919505457
33	23.473460855681257	30.90867551129739	22.290937930020274	23.32692570300108
34	21.56482736163195	25.691708946522546	30.501896028319624	22.241567663525878
35	21.525173583756132	31.127427809115645	23.899935923696678	23.44746268343155
36	22.344509921322704	27.01210096744714	29.496633367997564	21.1467557432326
37	20.896227901553587	33.68286431580164	23.466895660668705	21.95401212197607
38	21.563776930429942	26.161251693820315	30.481675227680967	21.793296148068784
39	21.883370623641003	29.95882309688127	24.64836815512768	23.509438124350048
40	21.851069864179244	23.495519910923434	28.05754262124602	26.595867603651296
41	20.837666362041617	31.655532095925377	24.394951627643145	23.111849914389857
42	22.324551728484543	26.781531318606287	28.673095305622958	22.22082164728621
43	21.14124097942205	32.598031491927436	23.764692906438093	22.49603462221242
44	20.96398071408313	24.374468219203983	31.893454763180284	22.7680963035326
45	22.155432304961188	30.32910009558924	23.83008224876312	23.68538535068646
46	22.50391285622748	25.02205905524218	30.014495950587715	22.459532137942624
47	21.549070893601822	31.414195527263942	25.66859946007836	21.368134119055874
48	21.118131492977867	26.328795470540655	30.19464490173216	22.358428134749314
49	24.824577989264593	27.12318406705953	25.96272019664072	22.08951774703516
50	21.471338984653197	23.700091387514576	31.625069591067135	23.203500036765092
51	21.49733715690291	29.47746299856091	24.43014107291043	24.59505877162575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3810.0
1	3579.0
2	3348.0
3	2926.0
4	2504.0
5	1865.0
6	1226.0
7	1482.5
8	1739.0
9	2395.0
10	3051.0
11	11112.5
12	19174.0
13	11840.0
14	4506.0
15	3345.5
16	2185.0
17	2470.0
18	2755.0
19	2266.5
20	1778.0
21	2157.5
22	2537.0
23	2887.5
24	3238.0
25	2576.5
26	2675.5
27	3436.0
28	4035.5
29	4635.0
30	5307.5
31	5980.0
32	6552.5
33	7125.0
34	7340.0
35	7555.0
36	8685.0
37	9815.0
38	10988.5
39	12162.0
40	12579.5
41	12997.0
42	14488.0
43	15979.0
44	18462.5
45	20946.0
46	22145.5
47	23345.0
48	25216.0
49	27087.0
50	26475.5
51	25864.0
52	24177.0
53	22490.0
54	21263.5
55	20037.0
56	19593.5
57	19150.0
58	17982.5
59	16815.0
60	16581.0
61	16347.0
62	14471.0
63	12595.0
64	11762.5
65	10930.0
66	9018.0
67	7106.0
68	6707.0
69	6308.0
70	5649.5
71	4991.0
72	4913.0
73	4835.0
74	4059.0
75	2778.0
76	2273.0
77	1769.5
78	1266.0
79	1058.5
80	851.0
81	635.0
82	419.0
83	325.0
84	231.0
85	160.5
86	90.0
87	71.0
88	52.0
89	34.5
90	17.0
91	15.0
92	13.0
93	7.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	380796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.605635817396205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12602090605182	19.455642810017157
2	8.614572201829143	4.583923414490623
3	3.3606599338618404	2.6823748291932885
4	1.9214691038080842	2.044876288411862
5	1.427591301456494	1.8990987131317079
6	1.0457000355842379	1.669290859259549
7	0.8527553550442877	1.5881668892349903
8	0.7437866154483338	1.5831132653177669
9	0.6598107071828323	1.5799215045352575
>10	7.815757740488731	45.58711892678187
>50	0.32990535358930784	5.464826133328947
>100	0.08697504776445387	4.54746093650954
>500	0.00899741873425385	1.6815258841091019
>1k	0.004998565963474361	2.421748366784779
>5k	0.0	0.0
>10k+	9.997131926948722E-4	3.2109111788935585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGTATATATATGTATATATACATATATATGTATATATACATATATATGTA	12072	3.1702013676614254	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2929	0.7691782476706688	No Hit
ATGTATATATACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGC	2314	0.6076744503618735	No Hit
ATGTATATATACATATATATATACATATATATGTATATATACATATATATG	1419	0.3726404689124886	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1381	0.3626613724934085	No Hit
ATGTATATATACATATATATGTATATATACATATATATGTATATATACATA	1062	0.2788894841332367	No Hit
ATGTATATATATGTATATATACATATATATGCTGTCTCTTATACACATCTG	927	0.24343743106545238	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	870	0.2284687864368323	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACATATATATACA	857	0.22505488503030496	No Hit
ATGTATATATATGTATATATACATATATATCTGTCTCTTATACACATCTGA	722	0.18960283196252062	No Hit
ATGTATATATATGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTAT	645	0.16938203132385843	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCC	631	0.16570552211682896	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACATATATATGCT	627	0.16465509091482053	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACCTGTCTCTTAT	538	0.1412829966701331	No Hit
ATGTATATATATGTATATATACCTGTCTCTTATACACATCTGACGCGATTC	505	0.13261693925356358	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.12788999884452568	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	444	0.11659786342293511	No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	416	0.10924484500887614	No Hit
ATGTATATATATGTATATATACATATATACTGTCTCTTATACACATCTGAC	400	0.10504312020084244	No Hit
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	391	0.10267964999632348	No Hit
ATGTATATATATGTATATATACATATATATGTATATATACATATATATCTG	381	0.10005357199130242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.626078005021061E-4	0.0	0.0	0.06591455792602864	0.0
2	2.626078005021061E-4	0.0	0.0	0.38734650574060653	0.0
3	2.626078005021061E-4	0.0	0.0	0.5105095641760943	0.0
4	2.626078005021061E-4	0.0	0.0	0.7085158457546823	0.0
5	2.626078005021061E-4	0.0	0.0	1.3626718768054287	0.0
6	2.626078005021061E-4	0.0	0.0	1.9078456706478009	0.0
7	2.626078005021061E-4	0.0	0.0	2.3592684797109214	0.0
8	2.626078005021061E-4	0.0	0.0	3.0210401369762288	0.0
9	2.626078005021061E-4	0.0	0.0	3.3548146514144057	0.0
10	2.626078005021061E-4	0.0	0.0	4.055189655353523	0.0
11	2.626078005021061E-4	0.0	0.0	4.8784651099276255	0.0
12	2.626078005021061E-4	0.0	0.0	6.10852004747949	0.0
13	2.626078005021061E-4	0.0	0.0	6.401065137238836	0.0
14	2.626078005021061E-4	0.0	0.0	6.70201367661425	0.0
15	2.626078005021061E-4	0.0	0.0	6.877435687349657	0.0
16	2.626078005021061E-4	0.0	0.0	7.240884883244572	0.0
17	2.626078005021061E-4	0.0	0.0	7.692570300108194	0.0
18	2.626078005021061E-4	0.0	0.0	8.206493765690816	0.0
19	5.252156010042122E-4	0.0	0.0	8.473040683200454	0.0
20	5.252156010042122E-4	0.0	0.0	8.753768421937206	0.0
21	5.252156010042122E-4	0.0	0.0	9.114328932026597	0.0
22	5.252156010042122E-4	0.0	0.0	9.481454637128541	0.0
23	7.878234015063183E-4	0.0	0.0	9.96885471486045	0.0
24	7.878234015063183E-4	0.0	0.0	10.254046786205738	0.0
25	7.878234015063183E-4	0.0	0.0	10.507200705889767	0.0
26	7.878234015063183E-4	0.0	0.0	10.737507746930115	0.0
27	7.878234015063183E-4	0.0	0.0	10.974379982983015	0.0
28	7.878234015063183E-4	0.0	0.0	11.260359877729808	0.0
29	7.878234015063183E-4	0.0	0.0	11.531633735648484	0.0
30	7.878234015063183E-4	0.0	0.0	11.904011596760471	0.0
31	7.878234015063183E-4	0.0	0.0	12.358585699429616	0.0
32	7.878234015063183E-4	0.0	0.0	12.88774041744136	0.0
33	7.878234015063183E-4	0.0	0.0	13.24068530131619	0.0
34	7.878234015063183E-4	0.0	0.0	13.532179959873528	0.0
35	7.878234015063183E-4	0.0	0.0	13.84914757507957	0.0
36	7.878234015063183E-4	0.0	0.0	14.08864588913749	0.0
37	7.878234015063183E-4	0.0	0.0	14.349678042836585	0.0
38	7.878234015063183E-4	0.0	0.0	14.597054590909568	0.0
39	0.0010504312020084244	0.0	0.0	14.910608304709083	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	30	2.1616634E-6	45.000004	1
CACGGGT	35	1.209064E-7	45.000004	4
GCAACTC	25	3.8858747E-5	45.000004	11
ATAGCGG	25	3.8858747E-5	45.000004	2
CAATTGG	35	1.209064E-7	45.000004	2
ACCAGTC	25	3.8858747E-5	45.000004	13
TCGTATA	25	3.8858747E-5	45.000004	29
TTAGTAG	30	2.1616634E-6	45.000004	1
CAATGCG	25	3.8858747E-5	45.000004	1
GCGTAAG	25	3.8858747E-5	45.000004	1
GTAGTAG	25	3.8858747E-5	45.000004	1
CCGTACT	20	7.0273376E-4	45.0	44
CCGTAAT	20	7.0273376E-4	45.0	27
CGTAGTG	20	7.0273376E-4	45.0	28
TACGAAC	20	7.0273376E-4	45.0	12
CGTACTC	20	7.0273376E-4	45.0	45
GAACGCC	20	7.0273376E-4	45.0	36
CTCGACG	20	7.0273376E-4	45.0	13
ACCGTAA	20	7.0273376E-4	45.0	26
GCGTGAA	20	7.0273376E-4	45.0	14
>>END_MODULE