SRR3553873_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431925
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	4745	1.0985703536493605	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4292	0.9936910343230885	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	3862	0.8941367135498061	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	3027	0.7008161139086647	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2785	0.6447878682641662	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2505	0.5799617989234243	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	2201	0.5095792093534757	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2128	0.4926781269896394	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	1540	0.35654338137408115	TruSeq Adapter, Index 16 (95% over 24bp)
AAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1522	0.35237599120217633	No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA	1113	0.2576836256294496	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	1107	0.25629449557214795	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1036	0.2398564565607455	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	940	0.21763037564391965	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	918	0.21253689876714704	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	855	0.19795103316548013	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	812	0.18799560108815186	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.18568038432598252	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	773	0.17896625571569139	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT	722	0.16715865022862764	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC	688	0.15928691323725183	No Hit
GAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	635	0.14701626439775425	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	608	0.14076517913989697	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	600	0.13891300573016147	No Hit
ACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	572	0.13243039879608728	TruSeq Adapter, Index 16 (95% over 23bp)
CTATATATATATATATATATATATATATATATATATATATATATATATATA	546	0.12641083521444696	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	517	0.11969670660415582	No Hit
ATATATATACATATATATATACATATATATGTATATATATATGTATATATA	494	0.1143717080511663	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	494	0.1143717080511663	No Hit
ATATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	474	0.10974127452682758	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	469	0.1085836661457429	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA	453	0.10487931932627192	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	30	2.1623418E-6	45.000004	41
ACCTGTA	30	2.1623418E-6	45.000004	14
CAATTCA	60	0.0	45.000004	17
CCGTACT	60	0.0	45.000004	44
GCACAAC	30	2.1623418E-6	45.000004	9
GTAGACG	30	2.1623418E-6	45.000004	1
ACGTGCG	30	2.1623418E-6	45.000004	1
GCATAAG	45	3.8380676E-10	45.0	1
GTGCACG	35	1.2095734E-7	45.0	1
TACCCGT	20	7.0283905E-4	45.0	24
GGTAATC	25	3.886748E-5	45.0	8
TACCAGG	35	1.2095734E-7	45.0	2
GGTCGAT	35	1.2095734E-7	45.0	8
ACGCATG	25	3.886748E-5	45.0	1
CATGATC	20	7.0283905E-4	45.0	26
AGCTTAC	20	7.0283905E-4	45.0	24
TTGTCGG	20	7.0283905E-4	45.0	2
TTCGTAG	25	3.886748E-5	45.0	1
TGCGGTT	25	3.886748E-5	45.0	34
TATGCGG	70	0.0	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content