##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431925
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92328992301904	33.0	31.0	34.0	30.0	34.0
2	32.06381431961567	33.0	31.0	34.0	30.0	34.0
3	32.12529490073508	34.0	31.0	34.0	30.0	34.0
4	35.69951264687156	37.0	35.0	37.0	33.0	37.0
5	35.5982496961278	37.0	35.0	37.0	33.0	37.0
6	35.55276263240146	37.0	35.0	37.0	33.0	37.0
7	35.5906974590496	37.0	35.0	37.0	33.0	37.0
8	35.30411529779476	37.0	35.0	37.0	33.0	37.0
9	37.24895525843607	39.0	37.0	39.0	34.0	39.0
10	36.88808936736702	39.0	37.0	39.0	33.0	39.0
11	36.883280662151996	39.0	37.0	39.0	33.0	39.0
12	36.81585923482086	39.0	35.0	39.0	33.0	39.0
13	36.639696706604155	39.0	35.0	39.0	32.0	39.0
14	37.745087688834865	40.0	36.0	41.0	33.0	41.0
15	37.84278057533137	40.0	36.0	41.0	33.0	41.0
16	37.97657232158361	40.0	37.0	41.0	33.0	41.0
17	37.864200960814955	40.0	36.0	41.0	33.0	41.0
18	37.814296463506395	39.0	36.0	41.0	33.0	41.0
19	37.72155119523065	39.0	36.0	41.0	33.0	41.0
20	37.59240377380332	39.0	35.0	41.0	33.0	41.0
21	37.54712739480234	39.0	35.0	41.0	33.0	41.0
22	37.50444174335822	39.0	35.0	41.0	33.0	41.0
23	37.385573884354926	39.0	35.0	41.0	32.0	41.0
24	37.369246975748105	39.0	35.0	41.0	32.0	41.0
25	37.30519650402269	39.0	35.0	41.0	32.0	41.0
26	37.24820281298837	39.0	35.0	41.0	32.0	41.0
27	37.19521676216936	39.0	35.0	41.0	32.0	41.0
28	37.08507032470915	39.0	35.0	41.0	32.0	41.0
29	37.027551079469816	39.0	35.0	41.0	32.0	41.0
30	36.94161486369161	39.0	35.0	41.0	31.0	41.0
31	36.91955084794814	39.0	35.0	41.0	31.0	41.0
32	36.76286623835156	39.0	35.0	41.0	31.0	41.0
33	36.623918504369975	39.0	35.0	41.0	31.0	41.0
34	36.590940556809635	39.0	35.0	41.0	31.0	41.0
35	36.455361463216995	39.0	35.0	41.0	30.0	41.0
36	36.37288418128147	39.0	35.0	41.0	30.0	41.0
37	36.29171731203334	39.0	35.0	40.0	30.0	41.0
38	36.21968860334549	39.0	35.0	40.0	30.0	41.0
39	36.14741911211437	39.0	35.0	40.0	30.0	41.0
40	35.99632575099844	38.0	35.0	40.0	29.0	41.0
41	35.821783874515255	38.0	35.0	40.0	29.0	41.0
42	35.7182983156798	38.0	35.0	40.0	29.0	41.0
43	35.61466921340511	38.0	35.0	40.0	28.0	41.0
44	35.54218672223187	38.0	35.0	40.0	28.0	41.0
45	35.485232389882505	38.0	35.0	40.0	28.0	41.0
46	35.372312322741216	38.0	34.0	40.0	27.0	41.0
47	35.178322625455806	37.0	34.0	40.0	27.0	41.0
48	35.066983851363084	37.0	34.0	40.0	26.0	41.0
49	35.03601551195231	37.0	34.0	40.0	26.0	41.0
50	34.91953927186433	37.0	34.0	40.0	26.0	41.0
51	33.51866643514499	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	2.0
13	8.0
14	17.0
15	19.0
16	42.0
17	91.0
18	146.0
19	322.0
20	605.0
21	980.0
22	1455.0
23	2139.0
24	2617.0
25	3242.0
26	3759.0
27	4587.0
28	5484.0
29	6654.0
30	8414.0
31	10758.0
32	14368.0
33	19171.0
34	30840.0
35	42561.0
36	36831.0
37	51576.0
38	82234.0
39	102960.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36036348903166	20.172020605429182	21.667419112114374	15.800196793424783
2	24.446142270070034	32.41442380042832	25.603287607802283	17.53614632169937
3	26.864849221508365	33.110841002488854	26.034844012270646	13.98946576373213
4	24.048619552005558	27.758291370029518	30.64814493256931	17.544944145395615
5	22.734965561150663	31.665914221218962	25.45603982172831	20.143080395902064
6	19.01533831104937	39.763847890258724	26.77131446431672	14.449499334375183
7	69.27498987092666	10.528679747641373	14.909301383342017	5.287028998089946
8	68.92654974822018	11.795797881576663	12.099091277420849	7.178561092782313
9	64.50610638421023	11.725878335359148	13.931353823001679	9.836661457428953
10	34.64837645424553	25.995948370666206	20.65080743184581	18.704867743242463
11	27.811078312206984	26.464779765005495	24.870058459223245	20.854083463564276
12	24.37251837703305	22.02581466689819	32.0108815187822	21.590785437286566
13	21.325461596341956	26.596052555420503	32.561903108178505	19.51658274005904
14	16.86728019910864	32.8763095444811	28.985124732303063	21.271285524107196
15	15.60479249869769	28.47739769635932	35.207964345661864	20.709845459281127
16	16.563986803264456	26.719916652196563	32.29310644209064	24.422990102448342
17	16.18220755918273	27.908780459570526	28.980262777102507	26.928749204144236
18	17.45696590843318	27.909011981246746	30.490710192741794	24.143311917578284
19	19.941425015917115	30.073045088846445	25.872315795566358	24.11321409967008
20	20.974937778549517	28.14284887422585	27.4408751519361	23.441338195288534
21	20.044915205186086	32.0574173757018	27.746252242866237	20.151415176245877
22	17.59680500086821	26.981767667997918	26.03924292411877	29.382184407015107
23	18.001504890895408	30.903281819760377	26.206864617699832	24.888348671644383
24	20.76402153151589	27.252879550847947	28.445910748393814	23.537188169242345
25	19.54969033975806	31.106557851478843	25.862360363489035	23.481391445274063
26	17.146032297273834	28.452161833651672	29.877640794119348	24.524165074955143
27	21.006887769867454	27.58696532962899	26.841234010534237	24.564912889969325
28	17.081437749609307	27.66545117786653	30.23140591537883	25.021705157145334
29	20.654511778665277	24.718874804653584	29.04601493314812	25.580598483533016
30	19.10401111304046	26.995195925218496	30.171210279562423	23.729582682178616
31	21.08282687966661	25.848932106268453	24.700816113908665	28.36742490015628
32	21.201829021242112	26.430977600277828	26.977831799502226	25.38936157897783
33	19.61405336574637	25.22498118886381	23.99629565318053	31.164669792209292
34	17.487758291370028	24.56282919488337	27.201713260404002	30.747699253342592
35	17.21873010360595	23.949528274584708	29.52943219308908	29.302309428720264
36	19.92058806505759	26.88198182554842	27.59576315332523	25.601666956068765
37	17.21201597499566	26.259188516524862	30.28998089946171	26.23881460901777
38	17.97140707298721	27.62655553626208	25.982983156798056	28.419054233952657
39	19.94466631938415	25.56855935636974	26.07188748046536	28.41488684378075
40	20.771893268507263	23.105168721421542	30.84771661746831	25.27522139260288
41	16.961509521328935	27.599699021820918	28.00254673843839	27.43624471841176
42	18.37263413787116	26.298084158129303	30.955605718585407	24.373675985414135
43	20.353070556230826	25.23933553278926	28.50263355906697	25.904960351912948
44	20.97100191005383	23.6978642125369	28.394049892921224	26.937083984488048
45	19.44249580366962	23.654338137408114	27.82033917925566	29.082826879666605
46	23.169300225733636	25.22613879724489	26.894252474387915	24.71030850263356
47	18.837761185390985	24.848063899982638	31.85969786421254	24.454477050413846
48	19.516814261735256	25.035596457718356	27.85738264745037	27.590206633096027
49	20.08172715170458	22.660878624761242	31.627018579614518	25.630375643919663
50	20.183133645887597	22.186953753545176	30.29553741969092	27.334375180876307
51	18.916941598657175	23.452451235746945	27.494125137465996	30.136482028129883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8870.0
1	7460.5
2	6051.0
3	4621.5
4	3192.0
5	2500.5
6	1809.0
7	3300.5
8	4792.0
9	4218.5
10	3645.0
11	2873.0
12	2101.0
13	2118.0
14	2135.0
15	2106.0
16	2077.0
17	1855.5
18	1634.0
19	1667.5
20	1701.0
21	1719.5
22	1738.0
23	1789.5
24	1841.0
25	2091.0
26	2321.5
27	2302.0
28	2943.5
29	3585.0
30	4213.5
31	4842.0
32	5508.5
33	6175.0
34	6835.5
35	7496.0
36	7557.0
37	7618.0
38	8419.0
39	9220.0
40	11228.0
41	13236.0
42	17185.0
43	21134.0
44	24572.0
45	28010.0
46	37745.0
47	47480.0
48	45313.5
49	43147.0
50	42559.0
51	41971.0
52	35494.0
53	29017.0
54	25938.5
55	22860.0
56	21214.5
57	19569.0
58	18239.5
59	16910.0
60	15584.0
61	14258.0
62	12931.0
63	11604.0
64	10389.5
65	9175.0
66	8030.5
67	6886.0
68	6247.5
69	5609.0
70	5317.5
71	5026.0
72	4657.0
73	4288.0
74	3534.0
75	2188.5
76	1597.0
77	1169.0
78	741.0
79	732.5
80	724.0
81	549.5
82	375.0
83	296.5
84	218.0
85	153.0
86	88.0
87	53.0
88	18.0
89	10.5
90	3.0
91	8.0
92	13.0
93	16.5
94	20.0
95	10.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.989023805486802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17183919831429	21.039863968238446
2	9.710903882760585	5.435974398947604
3	3.7157966665670075	3.120045640706774
4	1.9515310680616742	2.184857980845011
5	1.2215339293281309	1.7094771113587444
6	0.892378827765588	1.4986087352306066
7	0.687845445942296	1.3476485792682225
8	0.5330484407668179	1.1935604398480069
9	0.4097170168894209	1.0320821405307934
>10	4.717119720757133	30.901726713337336
>50	0.8641292949517065	15.631795848810235
>100	0.10180833647419532	5.208822495215255
>500	0.011587940736900278	2.3138996211621583
>1k	0.010760230684264543	7.381636326500786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	4745	1.0985703536493605	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4292	0.9936910343230885	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	3862	0.8941367135498061	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	3027	0.7008161139086647	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2785	0.6447878682641662	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2505	0.5799617989234243	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	2201	0.5095792093534757	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2128	0.4926781269896394	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	1540	0.35654338137408115	TruSeq Adapter, Index 16 (95% over 24bp)
AAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1522	0.35237599120217633	No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA	1113	0.2576836256294496	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	1107	0.25629449557214795	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1036	0.2398564565607455	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	940	0.21763037564391965	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	918	0.21253689876714704	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	855	0.19795103316548013	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	812	0.18799560108815186	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.18568038432598252	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	773	0.17896625571569139	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT	722	0.16715865022862764	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC	688	0.15928691323725183	No Hit
GAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	635	0.14701626439775425	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	608	0.14076517913989697	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	600	0.13891300573016147	No Hit
ACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	572	0.13243039879608728	TruSeq Adapter, Index 16 (95% over 23bp)
CTATATATATATATATATATATATATATATATATATATATATATATATATA	546	0.12641083521444696	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	517	0.11969670660415582	No Hit
ATATATATACATATATATATACATATATATGTATATATATATGTATATATA	494	0.1143717080511663	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	494	0.1143717080511663	No Hit
ATATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	474	0.10974127452682758	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	469	0.1085836661457429	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA	453	0.10487931932627192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6304335243387164E-4	0.0	0.0	0.3667303351276263	0.0
2	4.6304335243387164E-4	0.0	0.0	1.8294842854662268	0.0
3	4.6304335243387164E-4	0.0	0.0	2.2330265671123457	0.0
4	4.6304335243387164E-4	0.0	0.0	2.9278231174393703	0.0
5	4.6304335243387164E-4	0.0	0.0	5.834577762343	0.0
6	4.6304335243387164E-4	0.0	0.0	7.649476182207559	0.0
7	4.6304335243387164E-4	0.0	0.0	9.292585518319152	0.0
8	4.6304335243387164E-4	0.0	0.0	11.387856688082422	0.0
9	4.6304335243387164E-4	0.0	0.0	12.161602129999421	0.0
10	4.6304335243387164E-4	0.0	0.0	14.816924234531458	0.0
11	4.6304335243387164E-4	0.0	0.0	16.541529200671413	0.0
12	4.6304335243387164E-4	0.0	0.0	18.546738438386296	0.0
13	4.6304335243387164E-4	0.0	0.0	19.10609480812641	0.0
14	4.6304335243387164E-4	0.0	0.0	19.360537130288822	0.0
15	4.6304335243387164E-4	0.0	0.0	19.830294611332985	0.0
16	4.6304335243387164E-4	0.0	0.0	20.56908028014123	0.0
17	6.945650286508074E-4	0.0	0.0	21.39260288244487	0.0
18	6.945650286508074E-4	0.0	0.0	22.43236673033513	0.0
19	6.945650286508074E-4	0.0	0.0	23.107483938183712	0.0
20	6.945650286508074E-4	0.0	0.0	23.5557099033397	0.0
21	6.945650286508074E-4	0.0	0.0	24.05649128899693	0.0
22	6.945650286508074E-4	0.0	0.0	24.57046941019853	0.0
23	9.260867048677433E-4	0.0	0.0	25.13028882329108	0.0
24	9.260867048677433E-4	0.0	0.0	25.581293048561673	0.0
25	9.260867048677433E-4	0.0	0.0	25.960294032528797	0.0
26	9.260867048677433E-4	0.0	0.0	26.308039590206633	0.0
27	9.260867048677433E-4	0.0	0.0	26.693986224460264	0.0
28	9.260867048677433E-4	0.0	0.0	27.04427852057649	0.0
29	9.260867048677433E-4	0.0	0.0	27.42420559124848	0.0
30	9.260867048677433E-4	0.0	0.0	27.84372286855357	0.0
31	9.260867048677433E-4	0.0	0.0	28.22596515598773	0.0
32	9.260867048677433E-4	0.0	0.0	28.59547375122996	0.0
33	9.260867048677433E-4	0.0	0.0	28.958731261214332	0.0
34	9.260867048677433E-4	0.0	0.0	29.2905018232332	0.0
35	0.001157608381084679	0.0	0.0	29.688256062973895	0.0
36	0.001157608381084679	0.0	0.0	30.018405973259245	0.0
37	0.001157608381084679	0.0	0.0	30.3499450136019	0.0
38	0.001157608381084679	0.0	0.0	30.70093187474677	0.0
39	0.001157608381084679	0.0	0.0	31.010707877525032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	30	2.1623418E-6	45.000004	41
ACCTGTA	30	2.1623418E-6	45.000004	14
CAATTCA	60	0.0	45.000004	17
CCGTACT	60	0.0	45.000004	44
GCACAAC	30	2.1623418E-6	45.000004	9
GTAGACG	30	2.1623418E-6	45.000004	1
ACGTGCG	30	2.1623418E-6	45.000004	1
GCATAAG	45	3.8380676E-10	45.0	1
GTGCACG	35	1.2095734E-7	45.0	1
TACCCGT	20	7.0283905E-4	45.0	24
GGTAATC	25	3.886748E-5	45.0	8
TACCAGG	35	1.2095734E-7	45.0	2
GGTCGAT	35	1.2095734E-7	45.0	8
ACGCATG	25	3.886748E-5	45.0	1
CATGATC	20	7.0283905E-4	45.0	26
AGCTTAC	20	7.0283905E-4	45.0	24
TTGTCGG	20	7.0283905E-4	45.0	2
TTCGTAG	25	3.886748E-5	45.0	1
TGCGGTT	25	3.886748E-5	45.0	34
TATGCGG	70	0.0	45.0	2
>>END_MODULE